Experiment: H2 McCas with 0.1 mM sulfite
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01010 and MMP_RS01015 are separated by 250 nucleotides MMP_RS01015 and MMP_RS01020 are separated by 170 nucleotides MMP_RS01020 and MMP_RS01025 are separated by 120 nucleotides MMP_RS01025 and MMP_RS01030 are separated by 108 nucleotides
MMP_RS01010: MMP_RS01010 - DUF4352 domain-containing protein, at 191,240 to 191,773
_RS01010
MMP_RS01015: MMP_RS01015 - hypothetical protein, at 192,024 to 192,236
_RS01015
MMP_RS01020: MMP_RS01020 - 3 4-dihydroxy-2-butanone-4-phosphate synthase, at 192,407 to 193,150
_RS01020
MMP_RS01025: MMP_RS01025 - DUF120 domain-containing protein, at 193,271 to 193,654
_RS01025
MMP_RS01030: MMP_RS01030 - MTAP family purine nucleoside phosphorylase, at 193,763 to 194,524
_RS01030
Position (kb) 192
193
194 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 191.442 kb on + strand, within MMP_RS01010 at 191.478 kb on + strand, within MMP_RS01010 at 191.519 kb on + strand, within MMP_RS01010 at 191.522 kb on - strand, within MMP_RS01010 at 191.523 kb on + strand, within MMP_RS01010 at 191.572 kb on - strand, within MMP_RS01010 at 191.572 kb on - strand, within MMP_RS01010 at 191.715 kb on + strand, within MMP_RS01010 at 191.740 kb on - strand at 191.750 kb on - strand at 191.750 kb on - strand at 191.753 kb on - strand at 191.754 kb on + strand at 191.754 kb on + strand at 191.754 kb on + strand at 191.755 kb on - strand at 191.756 kb on + strand at 191.779 kb on - strand at 191.780 kb on + strand at 191.781 kb on - strand at 191.795 kb on - strand at 191.795 kb on - strand at 191.795 kb on - strand at 191.801 kb on + strand at 191.801 kb on + strand at 191.841 kb on + strand at 191.852 kb on + strand at 191.854 kb on - strand at 191.855 kb on + strand at 191.871 kb on + strand at 191.874 kb on - strand at 191.875 kb on + strand at 191.912 kb on - strand at 191.993 kb on - strand at 191.994 kb on + strand at 192.305 kb on - strand at 192.321 kb on - strand at 192.749 kb on + strand, within MMP_RS01020 at 193.106 kb on - strand at 193.107 kb on + strand at 193.165 kb on + strand at 193.170 kb on - strand at 193.193 kb on + strand at 193.198 kb on - strand at 193.198 kb on - strand at 193.198 kb on - strand at 193.199 kb on + strand at 193.199 kb on + strand at 193.199 kb on + strand at 193.199 kb on + strand at 193.199 kb on + strand at 193.200 kb on - strand at 193.200 kb on - strand at 193.201 kb on + strand at 193.201 kb on + strand at 193.201 kb on + strand at 193.201 kb on + strand at 193.201 kb on + strand at 193.201 kb on + strand at 193.203 kb on + strand at 193.229 kb on - strand at 193.305 kb on + strand at 193.431 kb on + strand at 193.672 kb on - strand at 193.672 kb on - strand at 193.674 kb on - strand at 193.677 kb on + strand at 193.677 kb on + strand at 193.677 kb on + strand at 193.698 kb on + strand at 193.711 kb on + strand at 193.714 kb on - strand at 193.740 kb on + strand at 193.760 kb on - strand at 193.857 kb on - strand, within MMP_RS01030 at 193.989 kb on - strand, within MMP_RS01030 at 193.989 kb on - strand, within MMP_RS01030 at 194.124 kb on - strand, within MMP_RS01030
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 0.1 mM sulfite remove 191,442 + MMP_RS01010 0.38 -0.4 191,478 + MMP_RS01010 0.45 +0.4 191,519 + MMP_RS01010 0.52 +0.2 191,522 - MMP_RS01010 0.53 -0.0 191,523 + MMP_RS01010 0.53 +0.0 191,572 - MMP_RS01010 0.62 -0.4 191,572 - MMP_RS01010 0.62 +0.3 191,715 + MMP_RS01010 0.89 -0.0 191,740 - -0.7 191,750 - +0.2 191,750 - +0.6 191,753 - -0.4 191,754 + +0.6 191,754 + +1.3 191,754 + +0.5 191,755 - +0.3 191,756 + +0.1 191,779 - +0.2 191,780 + +0.3 191,781 - -0.1 191,795 - -0.4 191,795 - +0.9 191,795 - +0.7 191,801 + +0.2 191,801 + +0.8 191,841 + +0.7 191,852 + +0.2 191,854 - -0.2 191,855 + +0.3 191,871 + -0.2 191,874 - +0.6 191,875 + -0.4 191,912 - -0.0 191,993 - -0.2 191,994 + +0.2 192,305 - +0.0 192,321 - +0.5 192,749 + MMP_RS01020 0.46 -2.6 193,106 - -2.3 193,107 + -2.8 193,165 + -1.8 193,170 - -0.2 193,193 + +0.2 193,198 - +0.1 193,198 - -0.8 193,198 - -0.1 193,199 + +0.3 193,199 + +0.7 193,199 + +0.6 193,199 + +0.1 193,199 + +0.9 193,200 - +0.4 193,200 - -0.2 193,201 + +0.7 193,201 + +0.0 193,201 + +0.5 193,201 + +0.5 193,201 + +0.2 193,201 + +0.7 193,203 + +0.7 193,229 - +0.7 193,305 + -1.9 193,431 + -2.7 193,672 - -2.2 193,672 - -2.6 193,674 - -0.3 193,677 + -4.4 193,677 + -1.6 193,677 + -4.5 193,698 + -0.2 193,711 + -0.9 193,714 - -1.3 193,740 + +0.9 193,760 - -0.7 193,857 - MMP_RS01030 0.12 -1.2 193,989 - MMP_RS01030 0.30 -0.4 193,989 - MMP_RS01030 0.30 -0.7 194,124 - MMP_RS01030 0.47 -0.3
Or see this region's nucleotide sequence