Experiment: H2 McCas with 0.1 mM sulfite
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00805 and MMP_RS00810 are separated by 45 nucleotides MMP_RS00810 and MMP_RS00815 are separated by 29 nucleotides
MMP_RS00805: MMP_RS00805 - carbamoyltransferase HypF, at 148,268 to 150,583
_RS00805
MMP_RS00810: MMP_RS00810 - topoisomerase DNA-binding C4 zinc finger domain-containing protein, at 150,629 to 151,501
_RS00810
MMP_RS00815: MMP_RS00815 - thiamine pyrophosphate-binding protein, at 151,531 to 153,036
_RS00815
Position (kb) 150
151
152 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 149.635 kb on + strand at 149.639 kb on - strand at 149.761 kb on - strand at 149.802 kb on - strand at 149.843 kb on + strand at 149.846 kb on + strand at 150.058 kb on - strand at 150.353 kb on - strand at 150.353 kb on - strand at 150.353 kb on - strand at 150.566 kb on - strand at 150.567 kb on + strand at 150.641 kb on - strand at 150.700 kb on - strand at 150.742 kb on - strand, within MMP_RS00810 at 150.812 kb on + strand, within MMP_RS00810 at 150.813 kb on - strand, within MMP_RS00810 at 150.813 kb on - strand, within MMP_RS00810 at 150.813 kb on - strand, within MMP_RS00810 at 150.843 kb on - strand, within MMP_RS00810 at 150.843 kb on - strand, within MMP_RS00810 at 150.847 kb on - strand, within MMP_RS00810 at 150.885 kb on + strand, within MMP_RS00810 at 151.021 kb on + strand, within MMP_RS00810 at 151.105 kb on - strand, within MMP_RS00810 at 151.159 kb on - strand, within MMP_RS00810 at 151.264 kb on + strand, within MMP_RS00810 at 151.277 kb on - strand, within MMP_RS00810 at 151.277 kb on - strand, within MMP_RS00810 at 151.570 kb on - strand at 151.608 kb on + strand at 151.743 kb on - strand at 151.839 kb on - strand at 151.998 kb on - strand at 152.020 kb on - strand at 152.128 kb on - strand at 152.140 kb on + strand at 152.154 kb on - strand at 152.165 kb on + strand at 152.179 kb on - strand at 152.179 kb on - strand at 152.185 kb on + strand at 152.185 kb on + strand at 152.185 kb on + strand at 152.420 kb on - strand at 152.426 kb on + strand at 152.438 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 0.1 mM sulfite remove 149,635 + -3.0 149,639 - -2.2 149,761 - -1.5 149,802 - -2.2 149,843 + -3.3 149,846 + -2.1 150,058 - -2.2 150,353 - -4.2 150,353 - -4.4 150,353 - -2.1 150,566 - -4.3 150,567 + -3.2 150,641 - -1.0 150,700 - -0.9 150,742 - MMP_RS00810 0.13 -0.7 150,812 + MMP_RS00810 0.21 -3.2 150,813 - MMP_RS00810 0.21 -2.0 150,813 - MMP_RS00810 0.21 -1.3 150,813 - MMP_RS00810 0.21 -2.1 150,843 - MMP_RS00810 0.25 -1.7 150,843 - MMP_RS00810 0.25 -1.6 150,847 - MMP_RS00810 0.25 -1.5 150,885 + MMP_RS00810 0.29 -2.9 151,021 + MMP_RS00810 0.45 -2.0 151,105 - MMP_RS00810 0.55 -0.8 151,159 - MMP_RS00810 0.61 -1.9 151,264 + MMP_RS00810 0.73 -2.7 151,277 - MMP_RS00810 0.74 -0.5 151,277 - MMP_RS00810 0.74 -1.0 151,570 - -4.1 151,608 + -4.9 151,743 - -2.8 151,839 - -1.4 151,998 - -0.0 152,020 - -2.3 152,128 - -1.6 152,140 + -1.0 152,154 - -2.0 152,165 + -1.8 152,179 - -2.0 152,179 - -3.0 152,185 + -1.9 152,185 + -2.1 152,185 + -2.0 152,420 - -1.2 152,426 + -2.6 152,438 + -1.7
Or see this region's nucleotide sequence