Experiment: BHIS with Spiramycin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3346 and BT3347 are separated by 23 nucleotides BT3347 and BT3348 are separated by 604 nucleotides
BT3346: BT3346 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 4,311,134 to 4,314,217
BT3346
BT3347: BT3347 - hypothetical protein (NCBI ptt file), at 4,314,241 to 4,315,650
BT3347
BT3348: BT3348 - unsaturated glucuronide lyase acting on chondroitin/heparin disaccharides (EC 3.2.1.180) (from data) , at 4,316,255 to 4,317,457
BT3348
Position (kb)
4314
4315
4316 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4313.281 kb on - strand, within BT3346 at 4313.303 kb on + strand, within BT3346 at 4313.307 kb on - strand, within BT3346 at 4313.473 kb on + strand, within BT3346 at 4313.473 kb on + strand, within BT3346 at 4313.474 kb on - strand at 4313.478 kb on + strand, within BT3346 at 4313.479 kb on - strand, within BT3346 at 4313.489 kb on + strand, within BT3346 at 4313.571 kb on + strand, within BT3346 at 4313.618 kb on - strand, within BT3346 at 4313.641 kb on + strand, within BT3346 at 4313.641 kb on + strand, within BT3346 at 4313.725 kb on + strand, within BT3346 at 4313.812 kb on + strand, within BT3346 at 4313.817 kb on - strand, within BT3346 at 4313.859 kb on - strand at 4313.986 kb on - strand at 4314.000 kb on + strand at 4314.000 kb on + strand at 4314.001 kb on - strand at 4314.027 kb on + strand at 4314.079 kb on + strand at 4314.080 kb on - strand at 4314.163 kb on - strand at 4314.163 kb on - strand at 4314.186 kb on - strand at 4314.186 kb on - strand at 4314.217 kb on + strand at 4314.221 kb on + strand at 4314.255 kb on + strand at 4314.256 kb on - strand at 4314.258 kb on - strand at 4314.279 kb on + strand at 4314.290 kb on + strand at 4314.290 kb on + strand at 4314.298 kb on - strand at 4314.298 kb on - strand at 4314.699 kb on + strand, within BT3347 at 4314.700 kb on - strand, within BT3347 at 4314.730 kb on + strand, within BT3347 at 4314.734 kb on - strand, within BT3347 at 4314.884 kb on + strand, within BT3347 at 4314.884 kb on + strand, within BT3347 at 4314.889 kb on + strand, within BT3347 at 4314.895 kb on - strand, within BT3347 at 4314.897 kb on - strand, within BT3347 at 4314.918 kb on - strand, within BT3347 at 4314.959 kb on + strand, within BT3347 at 4315.003 kb on - strand, within BT3347 at 4315.053 kb on + strand, within BT3347 at 4315.053 kb on + strand, within BT3347 at 4315.054 kb on - strand, within BT3347 at 4315.054 kb on - strand, within BT3347 at 4315.164 kb on - strand, within BT3347 at 4315.183 kb on - strand, within BT3347 at 4315.201 kb on - strand, within BT3347 at 4315.243 kb on - strand, within BT3347 at 4315.256 kb on - strand, within BT3347 at 4315.265 kb on - strand, within BT3347 at 4315.265 kb on - strand, within BT3347 at 4315.277 kb on - strand, within BT3347 at 4315.277 kb on - strand, within BT3347 at 4315.318 kb on - strand, within BT3347 at 4315.366 kb on + strand, within BT3347 at 4315.401 kb on - strand, within BT3347 at 4315.516 kb on - strand at 4315.516 kb on - strand at 4315.568 kb on + strand at 4315.568 kb on + strand at 4315.569 kb on - strand at 4315.587 kb on + strand at 4315.596 kb on + strand at 4315.613 kb on - strand at 4315.655 kb on - strand at 4315.694 kb on + strand at 4315.730 kb on - strand at 4315.738 kb on - strand at 4315.738 kb on - strand at 4315.740 kb on + strand at 4315.765 kb on + strand at 4315.810 kb on - strand at 4315.836 kb on + strand at 4315.837 kb on - strand at 4315.897 kb on + strand at 4315.897 kb on + strand at 4315.897 kb on + strand at 4315.898 kb on - strand at 4315.901 kb on + strand at 4316.044 kb on + strand at 4316.047 kb on + strand at 4316.048 kb on - strand at 4316.050 kb on - strand at 4316.189 kb on + strand at 4316.204 kb on + strand at 4316.231 kb on - strand at 4316.251 kb on + strand at 4316.364 kb on + strand at 4316.443 kb on + strand, within BT3348 at 4316.444 kb on - strand, within BT3348 at 4316.444 kb on - strand, within BT3348 at 4316.453 kb on + strand, within BT3348 at 4316.454 kb on - strand, within BT3348 at 4316.455 kb on + strand, within BT3348 at 4316.456 kb on - strand, within BT3348 at 4316.458 kb on - strand, within BT3348 at 4316.462 kb on - strand, within BT3348 at 4316.582 kb on + strand, within BT3348 at 4316.611 kb on + strand, within BT3348 at 4316.627 kb on + strand, within BT3348
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Spiramycin 0.2 mM remove 4,313,281 - BT3346 0.70 -2.6 4,313,303 + BT3346 0.70 -0.8 4,313,307 - BT3346 0.70 -0.1 4,313,473 + BT3346 0.76 -1.4 4,313,473 + BT3346 0.76 -0.6 4,313,474 - -0.1 4,313,478 + BT3346 0.76 -1.3 4,313,479 - BT3346 0.76 -2.6 4,313,489 + BT3346 0.76 -3.0 4,313,571 + BT3346 0.79 -1.7 4,313,618 - BT3346 0.81 -0.8 4,313,641 + BT3346 0.81 -0.3 4,313,641 + BT3346 0.81 -0.1 4,313,725 + BT3346 0.84 -0.6 4,313,812 + BT3346 0.87 -2.5 4,313,817 - BT3346 0.87 -2.5 4,313,859 - -1.1 4,313,986 - -1.9 4,314,000 + -3.0 4,314,000 + -2.3 4,314,001 - -0.1 4,314,027 + -2.1 4,314,079 + -1.1 4,314,080 - -0.1 4,314,163 - -1.9 4,314,163 - -1.8 4,314,186 - -1.7 4,314,186 - -2.1 4,314,217 + -0.7 4,314,221 + -1.7 4,314,255 + -0.2 4,314,256 - -1.4 4,314,258 - -1.8 4,314,279 + -0.1 4,314,290 + -1.3 4,314,290 + -1.3 4,314,298 - -3.7 4,314,298 - +0.4 4,314,699 + BT3347 0.32 -1.5 4,314,700 - BT3347 0.33 +0.4 4,314,730 + BT3347 0.35 -1.7 4,314,734 - BT3347 0.35 -2.1 4,314,884 + BT3347 0.46 -0.8 4,314,884 + BT3347 0.46 -0.7 4,314,889 + BT3347 0.46 -1.7 4,314,895 - BT3347 0.46 -1.6 4,314,897 - BT3347 0.47 -2.2 4,314,918 - BT3347 0.48 -2.3 4,314,959 + BT3347 0.51 -1.1 4,315,003 - BT3347 0.54 -1.1 4,315,053 + BT3347 0.58 -3.5 4,315,053 + BT3347 0.58 -1.1 4,315,054 - BT3347 0.58 -2.1 4,315,054 - BT3347 0.58 -0.1 4,315,164 - BT3347 0.65 -1.9 4,315,183 - BT3347 0.67 -2.8 4,315,201 - BT3347 0.68 -0.1 4,315,243 - BT3347 0.71 -0.1 4,315,256 - BT3347 0.72 -0.1 4,315,265 - BT3347 0.73 -1.1 4,315,265 - BT3347 0.73 -2.7 4,315,277 - BT3347 0.73 -1.1 4,315,277 - BT3347 0.73 -0.7 4,315,318 - BT3347 0.76 -0.2 4,315,366 + BT3347 0.80 -1.1 4,315,401 - BT3347 0.82 -0.1 4,315,516 - -3.3 4,315,516 - -1.1 4,315,568 + -2.7 4,315,568 + -1.1 4,315,569 - -2.0 4,315,587 + -0.1 4,315,596 + -0.1 4,315,613 - +0.4 4,315,655 - -1.1 4,315,694 + -0.5 4,315,730 - -1.2 4,315,738 - -1.4 4,315,738 - -1.7 4,315,740 + -0.1 4,315,765 + -0.1 4,315,810 - +0.3 4,315,836 + -2.1 4,315,837 - -0.7 4,315,897 + -1.9 4,315,897 + -1.7 4,315,897 + -2.1 4,315,898 - -0.1 4,315,901 + -0.8 4,316,044 + +1.1 4,316,047 + -3.1 4,316,048 - -0.6 4,316,050 - -2.6 4,316,189 + -1.1 4,316,204 + -3.1 4,316,231 - +1.4 4,316,251 + -0.4 4,316,364 + -1.1 4,316,443 + BT3348 0.16 -1.0 4,316,444 - BT3348 0.16 -1.1 4,316,444 - BT3348 0.16 -2.4 4,316,453 + BT3348 0.16 -1.1 4,316,454 - BT3348 0.17 -2.7 4,316,455 + BT3348 0.17 -0.5 4,316,456 - BT3348 0.17 -1.7 4,316,458 - BT3348 0.17 -0.1 4,316,462 - BT3348 0.17 -1.8 4,316,582 + BT3348 0.27 -1.5 4,316,611 + BT3348 0.30 -1.0 4,316,627 + BT3348 0.31 -1.7
Or see this region's nucleotide sequence