Experiment: BHIS with Spiramycin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3116 and BT3117 are separated by 11 nucleotides BT3117 and BT3118 are separated by 26 nucleotides BT3118 and BT3119 overlap by 4 nucleotides
BT3116: BT3116 - GTP-binding protein (NCBI ptt file), at 3,971,214 to 3,972,317
BT3116
BT3117: BT3117 - putative oxidoreductase (NCBI ptt file), at 3,972,329 to 3,973,240
BT3117
BT3118: BT3118 - prolipoprotein diacylglyceryl transferase (NCBI ptt file), at 3,973,267 to 3,974,112
BT3118
BT3119: BT3119 - chloramphenicol acetyltransferase (NCBI ptt file), at 3,974,109 to 3,974,753
BT3119
Position (kb)
3972
3973
3974 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3972.315 kb on + strand at 3972.328 kb on + strand at 3972.335 kb on + strand at 3972.335 kb on + strand at 3972.340 kb on + strand at 3972.340 kb on + strand at 3972.406 kb on + strand at 3972.406 kb on + strand at 3972.412 kb on + strand at 3972.441 kb on + strand, within BT3117 at 3972.487 kb on + strand, within BT3117 at 3972.487 kb on + strand, within BT3117 at 3972.502 kb on + strand, within BT3117 at 3972.711 kb on + strand, within BT3117 at 3972.929 kb on + strand, within BT3117 at 3973.143 kb on + strand, within BT3117 at 3974.073 kb on + strand at 3974.083 kb on + strand at 3974.122 kb on + strand at 3974.123 kb on - strand at 3974.127 kb on - strand at 3974.195 kb on + strand, within BT3119 at 3974.195 kb on + strand, within BT3119 at 3974.196 kb on - strand, within BT3119 at 3974.206 kb on + strand, within BT3119
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Spiramycin 0.2 mM remove 3,972,315 + -0.2 3,972,328 + -1.0 3,972,335 + +1.0 3,972,335 + -0.8 3,972,340 + +0.4 3,972,340 + -1.8 3,972,406 + -0.2 3,972,406 + -0.9 3,972,412 + -0.2 3,972,441 + BT3117 0.12 -0.2 3,972,487 + BT3117 0.17 -1.7 3,972,487 + BT3117 0.17 -1.2 3,972,502 + BT3117 0.19 -2.8 3,972,711 + BT3117 0.42 -2.3 3,972,929 + BT3117 0.66 -2.5 3,973,143 + BT3117 0.89 -0.2 3,974,073 + -0.2 3,974,083 + -1.0 3,974,122 + -0.7 3,974,123 - -1.1 3,974,127 - -1.9 3,974,195 + BT3119 0.13 -1.8 3,974,195 + BT3119 0.13 -1.2 3,974,196 - BT3119 0.13 -0.8 3,974,206 + BT3119 0.15 -2.2
Or see this region's nucleotide sequence