Experiment: BHIS with Spiramycin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1197 and BT1198 are separated by 242 nucleotides BT1198 and BT1199 are separated by 12 nucleotides BT1199 and BT1200 are separated by 95 nucleotides BT1200 and BT1201 are separated by 5 nucleotides
BT1197: BT1197 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 1,494,056 to 1,494,559
BT1197
BT1198: BT1198 - LemA protein (NCBI ptt file), at 1,494,802 to 1,495,362
BT1198
BT1199: BT1199 - putative protease (NCBI ptt file), at 1,495,375 to 1,496,337
BT1199
BT1200: BT1200 - hypothetical protein (NCBI ptt file), at 1,496,433 to 1,496,927
BT1200
BT1201: BT1201 - conserved hypothetical protein (NCBI ptt file), at 1,496,933 to 1,497,562
BT1201
Position (kb)
1495
1496
1497 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1494.382 kb on - strand, within BT1197 at 1494.496 kb on - strand, within BT1197 at 1494.814 kb on - strand at 1494.828 kb on - strand at 1494.976 kb on + strand, within BT1198 at 1495.121 kb on - strand, within BT1198 at 1495.236 kb on + strand, within BT1198 at 1495.269 kb on - strand, within BT1198 at 1495.276 kb on - strand, within BT1198 at 1495.375 kb on - strand at 1495.437 kb on - strand at 1495.437 kb on - strand at 1495.488 kb on - strand, within BT1199 at 1495.571 kb on - strand, within BT1199 at 1495.795 kb on + strand, within BT1199 at 1495.795 kb on + strand, within BT1199 at 1495.798 kb on - strand, within BT1199 at 1495.960 kb on + strand, within BT1199 at 1495.962 kb on + strand, within BT1199 at 1495.982 kb on + strand, within BT1199 at 1496.104 kb on + strand, within BT1199 at 1496.104 kb on + strand, within BT1199 at 1496.107 kb on - strand, within BT1199 at 1496.107 kb on - strand, within BT1199 at 1496.192 kb on + strand, within BT1199 at 1496.193 kb on - strand, within BT1199 at 1496.193 kb on - strand, within BT1199 at 1496.335 kb on + strand at 1496.391 kb on - strand at 1496.392 kb on + strand at 1496.406 kb on - strand at 1496.406 kb on - strand at 1496.406 kb on - strand at 1496.457 kb on - strand at 1496.472 kb on - strand at 1496.477 kb on - strand at 1496.576 kb on - strand, within BT1200 at 1496.614 kb on + strand, within BT1200 at 1496.615 kb on - strand, within BT1200 at 1496.632 kb on - strand, within BT1200 at 1496.668 kb on - strand, within BT1200 at 1496.748 kb on + strand, within BT1200 at 1496.966 kb on + strand at 1496.970 kb on - strand at 1496.970 kb on - strand at 1497.190 kb on + strand, within BT1201 at 1497.193 kb on + strand, within BT1201 at 1497.302 kb on - strand, within BT1201
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Spiramycin 0.2 mM remove 1,494,382 - BT1197 0.65 -0.7 1,494,496 - BT1197 0.87 +0.1 1,494,814 - +0.9 1,494,828 - +0.1 1,494,976 + BT1198 0.31 +0.9 1,495,121 - BT1198 0.57 -0.3 1,495,236 + BT1198 0.77 +0.7 1,495,269 - BT1198 0.83 -1.5 1,495,276 - BT1198 0.84 -3.3 1,495,375 - -1.2 1,495,437 - +0.1 1,495,437 - +0.1 1,495,488 - BT1199 0.12 -2.2 1,495,571 - BT1199 0.20 -1.9 1,495,795 + BT1199 0.44 -1.9 1,495,795 + BT1199 0.44 -2.2 1,495,798 - BT1199 0.44 -1.5 1,495,960 + BT1199 0.61 -2.2 1,495,962 + BT1199 0.61 -0.7 1,495,982 + BT1199 0.63 +1.1 1,496,104 + BT1199 0.76 -1.1 1,496,104 + BT1199 0.76 -1.9 1,496,107 - BT1199 0.76 -2.3 1,496,107 - BT1199 0.76 -1.0 1,496,192 + BT1199 0.85 -1.5 1,496,193 - BT1199 0.85 -2.0 1,496,193 - BT1199 0.85 -1.5 1,496,335 + -0.9 1,496,391 - -0.9 1,496,392 + -0.9 1,496,406 - +0.5 1,496,406 - +0.7 1,496,406 - -2.9 1,496,457 - +0.1 1,496,472 - -1.5 1,496,477 - -0.3 1,496,576 - BT1200 0.29 +0.1 1,496,614 + BT1200 0.37 -0.8 1,496,615 - BT1200 0.37 -1.3 1,496,632 - BT1200 0.40 -1.5 1,496,668 - BT1200 0.47 -0.1 1,496,748 + BT1200 0.64 +0.1 1,496,966 + -2.2 1,496,970 - -1.5 1,496,970 - +0.1 1,497,190 + BT1201 0.41 +0.5 1,497,193 + BT1201 0.41 -3.7 1,497,302 - BT1201 0.59 +0.1
Or see this region's nucleotide sequence