Experiment: BHIS with Spiramycin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0678 and BT0679 are separated by 13 nucleotides BT0679 and BT0680 are separated by 15 nucleotides
BT0678: BT0678 - cation efflux system protein czcC (NCBI ptt file), at 839,386 to 840,648
BT0678
BT0679: BT0679 - cation efflux system protein czcB (NCBI ptt file), at 840,662 to 841,798
BT0679
BT0680: BT0680 - cation efflux system protein czcA (NCBI ptt file), at 841,814 to 844,939
BT0680
Position (kb)
840
841
842 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 840.273 kb on - strand, within BT0678 at 840.313 kb on - strand, within BT0678 at 840.401 kb on - strand, within BT0678 at 840.403 kb on - strand, within BT0678 at 840.403 kb on - strand, within BT0678 at 840.539 kb on - strand at 840.548 kb on - strand at 840.549 kb on + strand at 840.572 kb on - strand at 840.616 kb on - strand at 840.636 kb on - strand at 840.649 kb on + strand at 840.802 kb on - strand, within BT0679 at 840.865 kb on - strand, within BT0679 at 841.099 kb on + strand, within BT0679 at 841.376 kb on - strand, within BT0679 at 841.692 kb on - strand at 841.692 kb on - strand at 841.776 kb on - strand at 841.778 kb on - strand at 841.811 kb on - strand at 841.814 kb on - strand at 841.834 kb on + strand at 841.835 kb on - strand at 841.836 kb on + strand at 842.269 kb on - strand, within BT0680 at 842.452 kb on + strand, within BT0680 at 842.461 kb on - strand, within BT0680 at 842.499 kb on + strand, within BT0680 at 842.725 kb on + strand, within BT0680 at 842.731 kb on - strand, within BT0680 at 842.752 kb on - strand, within BT0680 at 842.775 kb on + strand, within BT0680
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Spiramycin 0.2 mM remove 840,273 - BT0678 0.70 -2.0 840,313 - BT0678 0.73 -0.3 840,401 - BT0678 0.80 -2.7 840,403 - BT0678 0.81 -0.9 840,403 - BT0678 0.81 +1.7 840,539 - -0.9 840,548 - +0.1 840,549 + +0.1 840,572 - -0.9 840,616 - -1.1 840,636 - -1.5 840,649 + -2.2 840,802 - BT0679 0.12 -2.7 840,865 - BT0679 0.18 -1.5 841,099 + BT0679 0.38 -3.0 841,376 - BT0679 0.63 +0.1 841,692 - +0.1 841,692 - -0.9 841,776 - -0.2 841,778 - +0.1 841,811 - -0.0 841,814 - -0.9 841,834 + -1.7 841,835 - -1.2 841,836 + -1.9 842,269 - BT0680 0.15 +1.1 842,452 + BT0680 0.20 -0.7 842,461 - BT0680 0.21 -1.9 842,499 + BT0680 0.22 -1.5 842,725 + BT0680 0.29 +0.1 842,731 - BT0680 0.29 -0.7 842,752 - BT0680 0.30 -0.5 842,775 + BT0680 0.31 -1.9
Or see this region's nucleotide sequence