Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0819

Experiment: KB with Harmol 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0817 and Psyr_0818 are separated by 15 nucleotidesPsyr_0818 and Psyr_0819 are separated by 35 nucleotidesPsyr_0819 and Psyr_0820 are separated by 2 nucleotides Psyr_0817: Psyr_0817 - N-acetylmuramoyl-L-alanine amidase, family 2, at 923,198 to 923,746 _0817 Psyr_0818: Psyr_0818 - ampE protein, at 923,762 to 924,598 _0818 Psyr_0819: Psyr_0819 - TatD-related deoxyribonuclease, at 924,634 to 925,428 _0819 Psyr_0820: Psyr_0820 - transcriptional regulator, LacI family, at 925,431 to 926,426 _0820 Position (kb) 924 925 926Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 923.653 kb on + strand, within Psyr_0817at 923.654 kb on - strand, within Psyr_0817at 923.654 kb on - strand, within Psyr_0817at 923.654 kb on - strand, within Psyr_0817at 923.654 kb on - strand, within Psyr_0817at 923.779 kb on - strandat 923.976 kb on - strand, within Psyr_0818at 924.018 kb on - strand, within Psyr_0818at 924.171 kb on - strand, within Psyr_0818at 924.218 kb on + strand, within Psyr_0818at 924.219 kb on - strand, within Psyr_0818at 924.226 kb on - strand, within Psyr_0818at 924.226 kb on - strand, within Psyr_0818at 924.543 kb on + strandat 924.543 kb on + strandat 924.544 kb on - strandat 924.544 kb on - strandat 924.544 kb on - strandat 924.777 kb on - strand, within Psyr_0819at 924.923 kb on + strand, within Psyr_0819at 924.923 kb on + strand, within Psyr_0819at 924.923 kb on + strand, within Psyr_0819at 924.924 kb on - strand, within Psyr_0819at 924.924 kb on - strand, within Psyr_0819at 925.121 kb on + strand, within Psyr_0819at 925.121 kb on + strand, within Psyr_0819at 925.121 kb on + strand, within Psyr_0819at 925.121 kb on + strand, within Psyr_0819at 925.121 kb on + strand, within Psyr_0819at 925.124 kb on + strand, within Psyr_0819at 925.125 kb on - strand, within Psyr_0819at 925.125 kb on - strand, within Psyr_0819at 925.125 kb on - strand, within Psyr_0819at 925.229 kb on + strand, within Psyr_0819at 925.310 kb on + strand, within Psyr_0819at 925.310 kb on + strand, within Psyr_0819at 925.445 kb on + strandat 925.445 kb on + strandat 925.445 kb on + strandat 925.445 kb on + strandat 925.445 kb on + strandat 925.445 kb on + strandat 925.446 kb on - strandat 925.446 kb on - strandat 925.446 kb on - strandat 925.656 kb on + strand, within Psyr_0820at 925.657 kb on - strand, within Psyr_0820at 925.657 kb on - strand, within Psyr_0820at 925.684 kb on + strand, within Psyr_0820at 925.684 kb on + strand, within Psyr_0820at 925.684 kb on + strand, within Psyr_0820at 925.684 kb on + strand, within Psyr_0820at 925.684 kb on + strand, within Psyr_0820at 925.684 kb on + strand, within Psyr_0820at 925.685 kb on - strand, within Psyr_0820at 925.685 kb on - strand, within Psyr_0820at 925.685 kb on - strand, within Psyr_0820at 925.685 kb on - strand, within Psyr_0820at 925.728 kb on + strand, within Psyr_0820at 926.162 kb on + strand, within Psyr_0820at 926.200 kb on + strand, within Psyr_0820at 926.201 kb on - strand, within Psyr_0820at 926.201 kb on - strand, within Psyr_0820at 926.201 kb on - strand, within Psyr_0820at 926.246 kb on + strand, within Psyr_0820at 926.246 kb on + strand, within Psyr_0820at 926.246 kb on + strand, within Psyr_0820at 926.247 kb on - strand, within Psyr_0820at 926.371 kb on + strandat 926.371 kb on + strandat 926.371 kb on + strandat 926.371 kb on + strandat 926.371 kb on + strandat 926.381 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Harmol 10 mM
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923,653 + Psyr_0817 0.83 -2.9
923,654 - Psyr_0817 0.83 -1.0
923,654 - Psyr_0817 0.83 +0.3
923,654 - Psyr_0817 0.83 +0.2
923,654 - Psyr_0817 0.83 -0.0
923,779 - -0.1
923,976 - Psyr_0818 0.26 +0.2
924,018 - Psyr_0818 0.31 +1.1
924,171 - Psyr_0818 0.49 -0.3
924,218 + Psyr_0818 0.54 -0.5
924,219 - Psyr_0818 0.55 -0.7
924,226 - Psyr_0818 0.55 +0.4
924,226 - Psyr_0818 0.55 +0.2
924,543 + -1.0
924,543 + +0.9
924,544 - -0.1
924,544 - -2.4
924,544 - -0.1
924,777 - Psyr_0819 0.18 -1.0
924,923 + Psyr_0819 0.36 -0.4
924,923 + Psyr_0819 0.36 +0.2
924,923 + Psyr_0819 0.36 -1.4
924,924 - Psyr_0819 0.36 -1.2
924,924 - Psyr_0819 0.36 -0.6
925,121 + Psyr_0819 0.61 -2.5
925,121 + Psyr_0819 0.61 +0.0
925,121 + Psyr_0819 0.61 -1.4
925,121 + Psyr_0819 0.61 +0.0
925,121 + Psyr_0819 0.61 -0.8
925,124 + Psyr_0819 0.62 -2.1
925,125 - Psyr_0819 0.62 -1.6
925,125 - Psyr_0819 0.62 -0.8
925,125 - Psyr_0819 0.62 -0.1
925,229 + Psyr_0819 0.75 -0.4
925,310 + Psyr_0819 0.85 -2.0
925,310 + Psyr_0819 0.85 -0.0
925,445 + +0.5
925,445 + -0.8
925,445 + -1.8
925,445 + +0.7
925,445 + +0.0
925,445 + +1.2
925,446 - -0.5
925,446 - +0.9
925,446 - +1.4
925,656 + Psyr_0820 0.23 +2.5
925,657 - Psyr_0820 0.23 +0.3
925,657 - Psyr_0820 0.23 +0.8
925,684 + Psyr_0820 0.25 -0.4
925,684 + Psyr_0820 0.25 +2.2
925,684 + Psyr_0820 0.25 -0.8
925,684 + Psyr_0820 0.25 -1.1
925,684 + Psyr_0820 0.25 +0.7
925,684 + Psyr_0820 0.25 +2.4
925,685 - Psyr_0820 0.26 -0.0
925,685 - Psyr_0820 0.26 -0.3
925,685 - Psyr_0820 0.26 +1.6
925,685 - Psyr_0820 0.26 +0.3
925,728 + Psyr_0820 0.30 -0.1
926,162 + Psyr_0820 0.73 -1.2
926,200 + Psyr_0820 0.77 -0.1
926,201 - Psyr_0820 0.77 -0.6
926,201 - Psyr_0820 0.77 -1.1
926,201 - Psyr_0820 0.77 -0.5
926,246 + Psyr_0820 0.82 -0.1
926,246 + Psyr_0820 0.82 +0.7
926,246 + Psyr_0820 0.82 -0.4
926,247 - Psyr_0820 0.82 +1.2
926,371 + -0.3
926,371 + +0.0
926,371 + +2.2
926,371 + -2.0
926,371 + -0.0
926,381 + -1.0

Or see this region's nucleotide sequence