Experiment: KB with Harmol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0817 and Psyr_0818 are separated by 15 nucleotides Psyr_0818 and Psyr_0819 are separated by 35 nucleotides Psyr_0819 and Psyr_0820 are separated by 2 nucleotides
Psyr_0817: Psyr_0817 - N-acetylmuramoyl-L-alanine amidase, family 2, at 923,198 to 923,746
_0817
Psyr_0818: Psyr_0818 - ampE protein, at 923,762 to 924,598
_0818
Psyr_0819: Psyr_0819 - TatD-related deoxyribonuclease, at 924,634 to 925,428
_0819
Psyr_0820: Psyr_0820 - transcriptional regulator, LacI family, at 925,431 to 926,426
_0820
Position (kb)
924
925
926 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 923.653 kb on + strand, within Psyr_0817 at 923.654 kb on - strand, within Psyr_0817 at 923.654 kb on - strand, within Psyr_0817 at 923.654 kb on - strand, within Psyr_0817 at 923.654 kb on - strand, within Psyr_0817 at 923.779 kb on - strand at 923.976 kb on - strand, within Psyr_0818 at 924.018 kb on - strand, within Psyr_0818 at 924.171 kb on - strand, within Psyr_0818 at 924.218 kb on + strand, within Psyr_0818 at 924.219 kb on - strand, within Psyr_0818 at 924.226 kb on - strand, within Psyr_0818 at 924.226 kb on - strand, within Psyr_0818 at 924.543 kb on + strand at 924.543 kb on + strand at 924.544 kb on - strand at 924.544 kb on - strand at 924.544 kb on - strand at 924.777 kb on - strand, within Psyr_0819 at 924.923 kb on + strand, within Psyr_0819 at 924.923 kb on + strand, within Psyr_0819 at 924.923 kb on + strand, within Psyr_0819 at 924.924 kb on - strand, within Psyr_0819 at 924.924 kb on - strand, within Psyr_0819 at 925.121 kb on + strand, within Psyr_0819 at 925.121 kb on + strand, within Psyr_0819 at 925.121 kb on + strand, within Psyr_0819 at 925.121 kb on + strand, within Psyr_0819 at 925.121 kb on + strand, within Psyr_0819 at 925.124 kb on + strand, within Psyr_0819 at 925.125 kb on - strand, within Psyr_0819 at 925.125 kb on - strand, within Psyr_0819 at 925.125 kb on - strand, within Psyr_0819 at 925.229 kb on + strand, within Psyr_0819 at 925.310 kb on + strand, within Psyr_0819 at 925.310 kb on + strand, within Psyr_0819 at 925.445 kb on + strand at 925.445 kb on + strand at 925.445 kb on + strand at 925.445 kb on + strand at 925.445 kb on + strand at 925.445 kb on + strand at 925.446 kb on - strand at 925.446 kb on - strand at 925.446 kb on - strand at 925.656 kb on + strand, within Psyr_0820 at 925.657 kb on - strand, within Psyr_0820 at 925.657 kb on - strand, within Psyr_0820 at 925.684 kb on + strand, within Psyr_0820 at 925.684 kb on + strand, within Psyr_0820 at 925.684 kb on + strand, within Psyr_0820 at 925.684 kb on + strand, within Psyr_0820 at 925.684 kb on + strand, within Psyr_0820 at 925.684 kb on + strand, within Psyr_0820 at 925.685 kb on - strand, within Psyr_0820 at 925.685 kb on - strand, within Psyr_0820 at 925.685 kb on - strand, within Psyr_0820 at 925.685 kb on - strand, within Psyr_0820 at 925.728 kb on + strand, within Psyr_0820 at 926.162 kb on + strand, within Psyr_0820 at 926.200 kb on + strand, within Psyr_0820 at 926.201 kb on - strand, within Psyr_0820 at 926.201 kb on - strand, within Psyr_0820 at 926.201 kb on - strand, within Psyr_0820 at 926.246 kb on + strand, within Psyr_0820 at 926.246 kb on + strand, within Psyr_0820 at 926.246 kb on + strand, within Psyr_0820 at 926.247 kb on - strand, within Psyr_0820 at 926.371 kb on + strand at 926.371 kb on + strand at 926.371 kb on + strand at 926.371 kb on + strand at 926.371 kb on + strand at 926.381 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmol 10 mM remove 923,653 + Psyr_0817 0.83 -2.9 923,654 - Psyr_0817 0.83 -1.0 923,654 - Psyr_0817 0.83 +0.3 923,654 - Psyr_0817 0.83 +0.2 923,654 - Psyr_0817 0.83 -0.0 923,779 - -0.1 923,976 - Psyr_0818 0.26 +0.2 924,018 - Psyr_0818 0.31 +1.1 924,171 - Psyr_0818 0.49 -0.3 924,218 + Psyr_0818 0.54 -0.5 924,219 - Psyr_0818 0.55 -0.7 924,226 - Psyr_0818 0.55 +0.4 924,226 - Psyr_0818 0.55 +0.2 924,543 + -1.0 924,543 + +0.9 924,544 - -0.1 924,544 - -2.4 924,544 - -0.1 924,777 - Psyr_0819 0.18 -1.0 924,923 + Psyr_0819 0.36 -0.4 924,923 + Psyr_0819 0.36 +0.2 924,923 + Psyr_0819 0.36 -1.4 924,924 - Psyr_0819 0.36 -1.2 924,924 - Psyr_0819 0.36 -0.6 925,121 + Psyr_0819 0.61 -2.5 925,121 + Psyr_0819 0.61 +0.0 925,121 + Psyr_0819 0.61 -1.4 925,121 + Psyr_0819 0.61 +0.0 925,121 + Psyr_0819 0.61 -0.8 925,124 + Psyr_0819 0.62 -2.1 925,125 - Psyr_0819 0.62 -1.6 925,125 - Psyr_0819 0.62 -0.8 925,125 - Psyr_0819 0.62 -0.1 925,229 + Psyr_0819 0.75 -0.4 925,310 + Psyr_0819 0.85 -2.0 925,310 + Psyr_0819 0.85 -0.0 925,445 + +0.5 925,445 + -0.8 925,445 + -1.8 925,445 + +0.7 925,445 + +0.0 925,445 + +1.2 925,446 - -0.5 925,446 - +0.9 925,446 - +1.4 925,656 + Psyr_0820 0.23 +2.5 925,657 - Psyr_0820 0.23 +0.3 925,657 - Psyr_0820 0.23 +0.8 925,684 + Psyr_0820 0.25 -0.4 925,684 + Psyr_0820 0.25 +2.2 925,684 + Psyr_0820 0.25 -0.8 925,684 + Psyr_0820 0.25 -1.1 925,684 + Psyr_0820 0.25 +0.7 925,684 + Psyr_0820 0.25 +2.4 925,685 - Psyr_0820 0.26 -0.0 925,685 - Psyr_0820 0.26 -0.3 925,685 - Psyr_0820 0.26 +1.6 925,685 - Psyr_0820 0.26 +0.3 925,728 + Psyr_0820 0.30 -0.1 926,162 + Psyr_0820 0.73 -1.2 926,200 + Psyr_0820 0.77 -0.1 926,201 - Psyr_0820 0.77 -0.6 926,201 - Psyr_0820 0.77 -1.1 926,201 - Psyr_0820 0.77 -0.5 926,246 + Psyr_0820 0.82 -0.1 926,246 + Psyr_0820 0.82 +0.7 926,246 + Psyr_0820 0.82 -0.4 926,247 - Psyr_0820 0.82 +1.2 926,371 + -0.3 926,371 + +0.0 926,371 + +2.2 926,371 + -2.0 926,371 + -0.0 926,381 + -1.0
Or see this region's nucleotide sequence