Experiment: KB with Harmol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0432 and Psyr_0433 overlap by 4 nucleotides Psyr_0433 and Psyr_0434 overlap by 8 nucleotides Psyr_0434 and Psyr_0435 are separated by 1 nucleotides Psyr_0435 and Psyr_0436 overlap by 4 nucleotides Psyr_0436 and Psyr_0437 are separated by 9 nucleotides
Psyr_0432: Psyr_0432 - conserved hypothetical protein, at 471,117 to 472,064
_0432
Psyr_0433: Psyr_0433 - Glycosyl transferase, family 2, at 472,061 to 472,795
_0433
Psyr_0434: Psyr_0434 - AMP-dependent synthetase and ligase, at 472,788 to 474,479
_0434
Psyr_0435: Psyr_0435 - membrane protein, putative, at 474,481 to 475,026
_0435
Psyr_0436: Psyr_0436 - Phosphopantetheine-binding protein, at 475,023 to 475,277
_0436
Psyr_0437: Psyr_0437 - acyl carrier protein, putative, at 475,287 to 475,547
_0437
Position (kb)
472
473
474
475 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 471.878 kb on - strand, within Psyr_0432 at 471.923 kb on - strand, within Psyr_0432 at 471.923 kb on - strand, within Psyr_0432 at 471.923 kb on - strand, within Psyr_0432 at 471.937 kb on + strand, within Psyr_0432 at 472.192 kb on - strand, within Psyr_0433 at 472.399 kb on - strand, within Psyr_0433 at 472.407 kb on + strand, within Psyr_0433 at 472.408 kb on - strand, within Psyr_0433 at 472.629 kb on + strand, within Psyr_0433 at 472.629 kb on + strand, within Psyr_0433 at 472.630 kb on - strand, within Psyr_0433 at 472.630 kb on - strand, within Psyr_0433 at 472.630 kb on - strand, within Psyr_0433 at 472.630 kb on - strand, within Psyr_0433 at 472.630 kb on - strand, within Psyr_0433 at 472.634 kb on - strand, within Psyr_0433 at 472.658 kb on + strand, within Psyr_0433 at 472.789 kb on + strand at 472.789 kb on + strand at 472.789 kb on + strand at 472.789 kb on + strand at 472.789 kb on + strand at 472.789 kb on + strand at 472.789 kb on + strand at 472.790 kb on - strand at 472.790 kb on - strand at 472.790 kb on - strand at 472.790 kb on - strand at 473.951 kb on + strand, within Psyr_0434 at 473.951 kb on + strand, within Psyr_0434 at 473.951 kb on + strand, within Psyr_0434 at 473.951 kb on + strand, within Psyr_0434 at 473.951 kb on + strand, within Psyr_0434 at 473.951 kb on + strand, within Psyr_0434 at 473.951 kb on + strand, within Psyr_0434 at 473.951 kb on + strand, within Psyr_0434 at 473.952 kb on - strand, within Psyr_0434 at 473.952 kb on - strand, within Psyr_0434 at 473.952 kb on - strand, within Psyr_0434 at 474.661 kb on - strand, within Psyr_0435 at 474.669 kb on - strand, within Psyr_0435 at 475.049 kb on - strand, within Psyr_0436 at 475.049 kb on - strand, within Psyr_0436 at 475.059 kb on + strand, within Psyr_0436 at 475.060 kb on - strand, within Psyr_0436 at 475.460 kb on + strand, within Psyr_0437 at 475.461 kb on - strand, within Psyr_0437
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmol 10 mM remove 471,878 - Psyr_0432 0.80 +0.6 471,923 - Psyr_0432 0.85 -1.2 471,923 - Psyr_0432 0.85 +0.9 471,923 - Psyr_0432 0.85 +2.1 471,937 + Psyr_0432 0.86 +1.6 472,192 - Psyr_0433 0.18 -1.1 472,399 - Psyr_0433 0.46 +0.9 472,407 + Psyr_0433 0.47 -0.1 472,408 - Psyr_0433 0.47 -1.4 472,629 + Psyr_0433 0.77 -0.6 472,629 + Psyr_0433 0.77 +0.5 472,630 - Psyr_0433 0.77 -0.7 472,630 - Psyr_0433 0.77 +0.6 472,630 - Psyr_0433 0.77 +0.3 472,630 - Psyr_0433 0.77 +0.7 472,630 - Psyr_0433 0.77 +1.5 472,634 - Psyr_0433 0.78 -2.8 472,658 + Psyr_0433 0.81 +0.8 472,789 + +0.6 472,789 + -1.0 472,789 + +0.4 472,789 + +0.0 472,789 + +0.8 472,789 + -1.0 472,789 + +1.2 472,790 - +0.9 472,790 - -0.2 472,790 - -0.3 472,790 - -0.4 473,951 + Psyr_0434 0.69 +0.6 473,951 + Psyr_0434 0.69 -0.5 473,951 + Psyr_0434 0.69 +0.9 473,951 + Psyr_0434 0.69 +1.0 473,951 + Psyr_0434 0.69 +1.3 473,951 + Psyr_0434 0.69 +0.9 473,951 + Psyr_0434 0.69 +1.0 473,951 + Psyr_0434 0.69 -0.2 473,952 - Psyr_0434 0.69 +2.5 473,952 - Psyr_0434 0.69 +2.1 473,952 - Psyr_0434 0.69 -2.1 474,661 - Psyr_0435 0.33 -0.6 474,669 - Psyr_0435 0.34 +1.9 475,049 - Psyr_0436 0.10 +0.4 475,049 - Psyr_0436 0.10 +0.7 475,059 + Psyr_0436 0.14 +1.8 475,060 - Psyr_0436 0.15 -1.2 475,460 + Psyr_0437 0.66 +1.5 475,461 - Psyr_0437 0.67 -0.7
Or see this region's nucleotide sequence