Experiment: KB with Harmol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0252 and Psyr_0253 are separated by 37 nucleotides Psyr_0253 and Psyr_0254 are separated by 144 nucleotides
Psyr_0252: Psyr_0252 - adenylate cyclase, at 264,861 to 266,315
_0252
Psyr_0253: Psyr_0253 - acetylornithine deacetylase, at 266,353 to 267,540
_0253
Psyr_0254: Psyr_0254 - N-acetylglutamate synthase, at 267,685 to 268,983
_0254
Position (kb)
266
267
268 Strain fitness (log2 ratio)
-1
0
1
2
3 at 265.373 kb on + strand, within Psyr_0252 at 265.547 kb on - strand, within Psyr_0252 at 265.547 kb on - strand, within Psyr_0252 at 265.547 kb on - strand, within Psyr_0252 at 265.972 kb on + strand, within Psyr_0252 at 265.973 kb on - strand, within Psyr_0252 at 265.973 kb on - strand, within Psyr_0252 at 266.105 kb on - strand, within Psyr_0252 at 266.105 kb on - strand, within Psyr_0252 at 266.253 kb on - strand at 266.344 kb on + strand at 266.352 kb on + strand at 266.353 kb on - strand at 266.378 kb on + strand at 266.379 kb on - strand at 266.581 kb on + strand, within Psyr_0253 at 266.581 kb on + strand, within Psyr_0253 at 266.581 kb on + strand, within Psyr_0253 at 266.582 kb on - strand, within Psyr_0253 at 266.582 kb on - strand, within Psyr_0253 at 266.582 kb on - strand, within Psyr_0253 at 266.916 kb on + strand, within Psyr_0253 at 267.108 kb on + strand, within Psyr_0253 at 267.108 kb on + strand, within Psyr_0253 at 267.109 kb on - strand, within Psyr_0253 at 267.109 kb on - strand, within Psyr_0253 at 267.109 kb on - strand, within Psyr_0253 at 267.263 kb on - strand, within Psyr_0253 at 267.348 kb on + strand, within Psyr_0253 at 267.362 kb on + strand, within Psyr_0253 at 267.460 kb on + strand at 267.461 kb on - strand at 267.486 kb on + strand at 267.487 kb on - strand at 267.487 kb on - strand at 267.487 kb on - strand at 267.500 kb on + strand at 267.500 kb on + strand at 267.501 kb on - strand at 267.501 kb on - strand at 267.520 kb on + strand at 267.520 kb on + strand at 267.520 kb on + strand at 267.521 kb on - strand at 267.521 kb on - strand at 267.581 kb on + strand at 267.581 kb on + strand at 267.581 kb on + strand at 267.668 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Harmol 10 mM remove 265,373 + Psyr_0252 0.35 -0.6 265,547 - Psyr_0252 0.47 -0.7 265,547 - Psyr_0252 0.47 +0.5 265,547 - Psyr_0252 0.47 +0.5 265,972 + Psyr_0252 0.76 +0.3 265,973 - Psyr_0252 0.76 -0.1 265,973 - Psyr_0252 0.76 +0.7 266,105 - Psyr_0252 0.85 +0.6 266,105 - Psyr_0252 0.85 +1.8 266,253 - +1.4 266,344 + +3.1 266,352 + -0.6 266,353 - -0.5 266,378 + +1.3 266,379 - +0.9 266,581 + Psyr_0253 0.19 +0.7 266,581 + Psyr_0253 0.19 +0.3 266,581 + Psyr_0253 0.19 +1.3 266,582 - Psyr_0253 0.19 +0.9 266,582 - Psyr_0253 0.19 +0.6 266,582 - Psyr_0253 0.19 +0.4 266,916 + Psyr_0253 0.47 +1.0 267,108 + Psyr_0253 0.64 -0.4 267,108 + Psyr_0253 0.64 -0.1 267,109 - Psyr_0253 0.64 +0.6 267,109 - Psyr_0253 0.64 -1.4 267,109 - Psyr_0253 0.64 +0.5 267,263 - Psyr_0253 0.77 -0.0 267,348 + Psyr_0253 0.84 +0.5 267,362 + Psyr_0253 0.85 +0.5 267,460 + -0.5 267,461 - -1.5 267,486 + +2.1 267,487 - +2.1 267,487 - -0.8 267,487 - +0.5 267,500 + +0.9 267,500 + +0.2 267,501 - +0.5 267,501 - +0.5 267,520 + +0.2 267,520 + -0.5 267,520 + -0.1 267,521 - -1.7 267,521 - -0.2 267,581 + +0.9 267,581 + -0.3 267,581 + +0.8 267,668 + -0.3
Or see this region's nucleotide sequence