Experiment: BHIS with Geneticin G418 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1773 and BT1774 are separated by 11 nucleotides BT1774 and BT1775 are separated by 36 nucleotides
BT1773: BT1773 - hypothetical protein (NCBI ptt file), at 2,202,186 to 2,204,192
BT1773
BT1774: BT1774 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 2,204,204 to 2,207,509
BT1774
BT1775: BT1775 - hypothetical protein (NCBI ptt file), at 2,207,546 to 2,209,258
BT1775
Position (kb)
2204
2205
2206
2207
2208 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2203.265 kb on + strand, within BT1773 at 2203.312 kb on + strand, within BT1773 at 2203.336 kb on + strand, within BT1773 at 2203.349 kb on - strand, within BT1773 at 2203.465 kb on + strand, within BT1773 at 2203.559 kb on + strand, within BT1773 at 2203.590 kb on + strand, within BT1773 at 2203.595 kb on + strand, within BT1773 at 2203.595 kb on + strand, within BT1773 at 2203.662 kb on - strand, within BT1773 at 2203.714 kb on + strand, within BT1773 at 2203.717 kb on + strand, within BT1773 at 2203.717 kb on + strand, within BT1773 at 2203.717 kb on + strand, within BT1773 at 2203.882 kb on + strand, within BT1773 at 2203.920 kb on + strand, within BT1773 at 2203.925 kb on - strand, within BT1773 at 2203.985 kb on + strand, within BT1773 at 2204.082 kb on + strand at 2204.090 kb on - strand at 2204.132 kb on + strand at 2204.164 kb on - strand at 2204.173 kb on - strand at 2204.336 kb on - strand at 2204.336 kb on - strand at 2204.482 kb on + strand at 2204.484 kb on + strand at 2204.514 kb on + strand at 2204.522 kb on + strand at 2204.783 kb on - strand, within BT1774 at 2204.806 kb on + strand, within BT1774 at 2204.935 kb on - strand, within BT1774 at 2204.935 kb on - strand, within BT1774 at 2204.935 kb on - strand, within BT1774 at 2205.015 kb on + strand, within BT1774 at 2205.411 kb on + strand, within BT1774 at 2205.460 kb on + strand, within BT1774 at 2205.697 kb on + strand, within BT1774 at 2205.784 kb on + strand, within BT1774 at 2205.784 kb on + strand, within BT1774 at 2205.799 kb on + strand, within BT1774 at 2205.853 kb on - strand, within BT1774 at 2205.859 kb on - strand, within BT1774 at 2205.888 kb on + strand, within BT1774 at 2205.918 kb on + strand, within BT1774 at 2206.000 kb on + strand, within BT1774 at 2206.078 kb on - strand, within BT1774 at 2206.159 kb on + strand, within BT1774 at 2206.276 kb on + strand, within BT1774 at 2206.352 kb on - strand, within BT1774 at 2206.354 kb on - strand, within BT1774 at 2206.387 kb on + strand, within BT1774 at 2206.403 kb on - strand, within BT1774 at 2206.415 kb on + strand, within BT1774 at 2206.416 kb on - strand, within BT1774 at 2206.429 kb on - strand, within BT1774 at 2206.429 kb on - strand, within BT1774 at 2206.432 kb on + strand, within BT1774 at 2206.451 kb on - strand, within BT1774 at 2206.453 kb on + strand, within BT1774 at 2206.461 kb on + strand, within BT1774 at 2206.461 kb on + strand, within BT1774 at 2206.462 kb on - strand, within BT1774 at 2206.470 kb on - strand, within BT1774 at 2206.470 kb on - strand, within BT1774 at 2206.480 kb on + strand, within BT1774 at 2206.503 kb on - strand, within BT1774 at 2206.559 kb on - strand, within BT1774 at 2206.564 kb on - strand, within BT1774 at 2206.713 kb on + strand, within BT1774 at 2206.714 kb on - strand, within BT1774 at 2206.719 kb on + strand, within BT1774 at 2206.797 kb on + strand, within BT1774 at 2206.873 kb on + strand, within BT1774 at 2206.932 kb on - strand, within BT1774 at 2206.935 kb on + strand, within BT1774 at 2207.051 kb on - strand, within BT1774 at 2207.074 kb on + strand, within BT1774 at 2207.074 kb on + strand, within BT1774 at 2207.189 kb on + strand at 2207.237 kb on - strand at 2207.251 kb on - strand at 2207.317 kb on + strand at 2207.318 kb on - strand at 2207.329 kb on + strand at 2207.334 kb on - strand at 2207.362 kb on + strand at 2207.362 kb on + strand at 2207.512 kb on + strand at 2207.744 kb on - strand, within BT1775 at 2207.763 kb on + strand, within BT1775 at 2207.940 kb on - strand, within BT1775 at 2207.952 kb on + strand, within BT1775 at 2208.048 kb on + strand, within BT1775 at 2208.051 kb on + strand, within BT1775 at 2208.075 kb on + strand, within BT1775 at 2208.142 kb on + strand, within BT1775 at 2208.220 kb on - strand, within BT1775 at 2208.242 kb on + strand, within BT1775 at 2208.262 kb on + strand, within BT1775 at 2208.489 kb on + strand, within BT1775 at 2208.489 kb on + strand, within BT1775
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Geneticin G418 0.2 mM remove 2,203,265 + BT1773 0.54 -1.5 2,203,312 + BT1773 0.56 -1.9 2,203,336 + BT1773 0.57 -1.2 2,203,349 - BT1773 0.58 -0.9 2,203,465 + BT1773 0.64 -0.7 2,203,559 + BT1773 0.68 -0.8 2,203,590 + BT1773 0.70 -2.0 2,203,595 + BT1773 0.70 -0.6 2,203,595 + BT1773 0.70 -1.2 2,203,662 - BT1773 0.74 -0.5 2,203,714 + BT1773 0.76 +0.1 2,203,717 + BT1773 0.76 -1.2 2,203,717 + BT1773 0.76 +0.1 2,203,717 + BT1773 0.76 +0.1 2,203,882 + BT1773 0.85 +0.1 2,203,920 + BT1773 0.86 -1.9 2,203,925 - BT1773 0.87 +0.1 2,203,985 + BT1773 0.90 -3.5 2,204,082 + -0.9 2,204,090 - -3.8 2,204,132 + -1.1 2,204,164 - -0.5 2,204,173 - -2.7 2,204,336 - +0.1 2,204,336 - -1.7 2,204,482 + -2.1 2,204,484 + -0.9 2,204,514 + +0.1 2,204,522 + -1.5 2,204,783 - BT1774 0.18 +1.7 2,204,806 + BT1774 0.18 -1.9 2,204,935 - BT1774 0.22 -1.9 2,204,935 - BT1774 0.22 -2.5 2,204,935 - BT1774 0.22 -0.5 2,205,015 + BT1774 0.25 -1.5 2,205,411 + BT1774 0.37 +0.7 2,205,460 + BT1774 0.38 -2.2 2,205,697 + BT1774 0.45 +0.1 2,205,784 + BT1774 0.48 -1.8 2,205,784 + BT1774 0.48 -1.5 2,205,799 + BT1774 0.48 -0.9 2,205,853 - BT1774 0.50 -1.2 2,205,859 - BT1774 0.50 -2.7 2,205,888 + BT1774 0.51 -0.6 2,205,918 + BT1774 0.52 -1.2 2,206,000 + BT1774 0.54 -1.5 2,206,078 - BT1774 0.57 +0.1 2,206,159 + BT1774 0.59 -2.0 2,206,276 + BT1774 0.63 -0.6 2,206,352 - BT1774 0.65 -0.8 2,206,354 - BT1774 0.65 -2.8 2,206,387 + BT1774 0.66 -2.2 2,206,403 - BT1774 0.67 +0.1 2,206,415 + BT1774 0.67 -1.1 2,206,416 - BT1774 0.67 -1.9 2,206,429 - BT1774 0.67 -1.3 2,206,429 - BT1774 0.67 +0.1 2,206,432 + BT1774 0.67 +1.1 2,206,451 - BT1774 0.68 -1.5 2,206,453 + BT1774 0.68 -2.0 2,206,461 + BT1774 0.68 -2.2 2,206,461 + BT1774 0.68 -1.0 2,206,462 - BT1774 0.68 -2.5 2,206,470 - BT1774 0.69 -1.9 2,206,470 - BT1774 0.69 -2.4 2,206,480 + BT1774 0.69 -1.5 2,206,503 - BT1774 0.70 +0.1 2,206,559 - BT1774 0.71 -0.6 2,206,564 - BT1774 0.71 -1.9 2,206,713 + BT1774 0.76 -0.3 2,206,714 - BT1774 0.76 +0.4 2,206,719 + BT1774 0.76 +0.1 2,206,797 + BT1774 0.78 -3.9 2,206,873 + BT1774 0.81 -2.4 2,206,932 - BT1774 0.83 -1.5 2,206,935 + BT1774 0.83 -0.5 2,207,051 - BT1774 0.86 -0.5 2,207,074 + BT1774 0.87 -2.5 2,207,074 + BT1774 0.87 +0.1 2,207,189 + -0.9 2,207,237 - -1.5 2,207,251 - -0.9 2,207,317 + -2.2 2,207,318 - +2.1 2,207,329 + -1.3 2,207,334 - +0.1 2,207,362 + -1.9 2,207,362 + -2.5 2,207,512 + -1.0 2,207,744 - BT1775 0.12 +1.7 2,207,763 + BT1775 0.13 -2.1 2,207,940 - BT1775 0.23 +1.1 2,207,952 + BT1775 0.24 -0.5 2,208,048 + BT1775 0.29 -0.4 2,208,051 + BT1775 0.29 -0.9 2,208,075 + BT1775 0.31 -1.2 2,208,142 + BT1775 0.35 +0.2 2,208,220 - BT1775 0.39 -0.9 2,208,242 + BT1775 0.41 +0.1 2,208,262 + BT1775 0.42 -1.5 2,208,489 + BT1775 0.55 -2.9 2,208,489 + BT1775 0.55 -1.3
Or see this region's nucleotide sequence