Experiment: BHIS with Geneticin G418 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0627 and BT0628 are separated by 7 nucleotides BT0628 and BT0629 are separated by 161 nucleotides BT0629 and BT0630 are separated by 30 nucleotides BT0630 and BT0631 are separated by 14 nucleotides
BT0627: BT0627 - conserved hypothetical protein, with a conserved domain of unknown function (NCBI ptt file), at 774,261 to 775,004
BT0627
BT0628: BT0628 - conserved hypothetical protein (NCBI ptt file), at 775,012 to 775,257
BT0628
BT0629: BT0629 - Mn2+ and Fe2+ transport protein (NCBI ptt file), at 775,419 to 776,675
BT0629
BT0630: BT0630 - exodeoxyribonuclease (NCBI ptt file), at 776,706 to 777,467
BT0630
BT0631: BT0631 - conserved hypothetical protein (NCBI ptt file), at 777,482 to 777,940
BT0631
Position (kb) 775
776
777 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 775.018 kb on + strand at 775.027 kb on - strand at 775.110 kb on + strand, within BT0628 at 775.110 kb on + strand, within BT0628 at 775.281 kb on + strand at 775.320 kb on + strand at 775.385 kb on - strand at 775.386 kb on + strand at 775.387 kb on - strand at 775.387 kb on - strand at 775.387 kb on - strand at 775.391 kb on - strand at 775.391 kb on - strand at 775.393 kb on - strand at 775.490 kb on + strand at 775.495 kb on - strand at 775.637 kb on + strand, within BT0629 at 775.695 kb on + strand, within BT0629 at 775.814 kb on + strand, within BT0629 at 775.817 kb on + strand, within BT0629 at 775.931 kb on + strand, within BT0629 at 775.958 kb on - strand, within BT0629 at 776.010 kb on - strand, within BT0629 at 776.080 kb on + strand, within BT0629 at 776.080 kb on + strand, within BT0629 at 776.083 kb on + strand, within BT0629 at 776.120 kb on + strand, within BT0629 at 776.217 kb on - strand, within BT0629 at 776.292 kb on - strand, within BT0629 at 776.345 kb on + strand, within BT0629 at 776.347 kb on + strand, within BT0629 at 776.385 kb on - strand, within BT0629 at 776.405 kb on + strand, within BT0629 at 776.523 kb on - strand, within BT0629 at 776.610 kb on - strand at 776.617 kb on + strand at 776.622 kb on + strand at 776.627 kb on + strand at 776.871 kb on + strand, within BT0630 at 776.871 kb on + strand, within BT0630 at 776.872 kb on - strand, within BT0630 at 776.872 kb on - strand, within BT0630 at 776.918 kb on + strand, within BT0630 at 776.921 kb on + strand, within BT0630 at 777.016 kb on + strand, within BT0630 at 777.027 kb on + strand, within BT0630 at 777.099 kb on + strand, within BT0630 at 777.152 kb on + strand, within BT0630 at 777.153 kb on - strand, within BT0630 at 777.176 kb on + strand, within BT0630 at 777.264 kb on - strand, within BT0630 at 777.306 kb on - strand, within BT0630 at 777.306 kb on - strand, within BT0630 at 777.460 kb on + strand at 777.491 kb on - strand at 777.491 kb on - strand at 777.546 kb on + strand, within BT0631 at 777.569 kb on + strand, within BT0631 at 777.598 kb on + strand, within BT0631
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Geneticin G418 0.2 mM remove 775,018 + +0.8 775,027 - -2.4 775,110 + BT0628 0.40 -1.8 775,110 + BT0628 0.40 +0.7 775,281 + -1.3 775,320 + +0.2 775,385 - -2.1 775,386 + -1.9 775,387 - -0.8 775,387 - -0.8 775,387 - +0.8 775,391 - -0.6 775,391 - +0.2 775,393 - -0.8 775,490 + -2.4 775,495 - -2.1 775,637 + BT0629 0.17 -1.8 775,695 + BT0629 0.22 -2.6 775,814 + BT0629 0.31 -0.8 775,817 + BT0629 0.32 +0.2 775,931 + BT0629 0.41 -2.1 775,958 - BT0629 0.43 -0.8 776,010 - BT0629 0.47 -1.4 776,080 + BT0629 0.53 -0.8 776,080 + BT0629 0.53 -3.1 776,083 + BT0629 0.53 -1.7 776,120 + BT0629 0.56 -1.8 776,217 - BT0629 0.63 -0.8 776,292 - BT0629 0.69 -0.2 776,345 + BT0629 0.74 +0.2 776,347 + BT0629 0.74 -0.8 776,385 - BT0629 0.77 -1.4 776,405 + BT0629 0.78 -2.1 776,523 - BT0629 0.88 +0.2 776,610 - -1.4 776,617 + -0.1 776,622 + +0.2 776,627 + -0.4 776,871 + BT0630 0.22 -2.1 776,871 + BT0630 0.22 -1.4 776,872 - BT0630 0.22 -3.0 776,872 - BT0630 0.22 +1.5 776,918 + BT0630 0.28 -1.8 776,921 + BT0630 0.28 -2.1 777,016 + BT0630 0.41 -1.8 777,027 + BT0630 0.42 -0.5 777,099 + BT0630 0.52 -1.1 777,152 + BT0630 0.59 -2.6 777,153 - BT0630 0.59 -3.0 777,176 + BT0630 0.62 +0.2 777,264 - BT0630 0.73 -0.8 777,306 - BT0630 0.79 +0.2 777,306 - BT0630 0.79 -2.5 777,460 + +0.2 777,491 - -0.8 777,491 - +0.2 777,546 + BT0631 0.14 +0.8 777,569 + BT0631 0.19 -1.4 777,598 + BT0631 0.25 -0.8
Or see this region's nucleotide sequence