Experiment: BHIS with Geneticin G418 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0506 and BT0507 are separated by 130 nucleotides BT0507 and BT0508 are separated by 61 nucleotides BT0508 and BT0509 overlap by 4 nucleotides
BT0506: BT0506 - beta-hexosaminidase precursor (NCBI ptt file), at 626,800 to 629,103
BT0506
BT0507: BT0507 - transcriptional regulator (TetR/AcrR family) (NCBI ptt file), at 629,234 to 629,842
BT0507
BT0508: BT0508 - ABC transporter ATP-binding protein (NCBI ptt file), at 629,904 to 631,733
BT0508
BT0509: BT0509 - ABC transporter ATP-binding/transmembrane protein (NCBI ptt file), at 631,730 to 633,460
BT0509
Position (kb) 629
630
631
632 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 628.959 kb on + strand at 628.961 kb on + strand at 629.046 kb on - strand at 629.135 kb on + strand at 629.136 kb on - strand at 629.139 kb on - strand at 629.140 kb on + strand at 629.140 kb on + strand at 629.141 kb on - strand at 629.164 kb on + strand at 629.172 kb on + strand at 629.308 kb on + strand, within BT0507 at 629.309 kb on - strand, within BT0507 at 629.309 kb on - strand, within BT0507 at 629.350 kb on + strand, within BT0507 at 629.350 kb on + strand, within BT0507 at 629.442 kb on + strand, within BT0507 at 629.442 kb on + strand, within BT0507 at 629.442 kb on + strand, within BT0507 at 629.443 kb on - strand, within BT0507 at 629.443 kb on - strand, within BT0507 at 629.444 kb on + strand, within BT0507 at 629.446 kb on + strand, within BT0507 at 629.447 kb on - strand, within BT0507 at 629.473 kb on - strand, within BT0507 at 629.558 kb on - strand, within BT0507 at 629.629 kb on + strand, within BT0507 at 629.633 kb on - strand, within BT0507 at 629.796 kb on - strand at 629.796 kb on - strand at 629.839 kb on - strand at 629.900 kb on - strand at 630.091 kb on + strand, within BT0508 at 630.116 kb on + strand, within BT0508 at 630.172 kb on - strand, within BT0508 at 630.173 kb on - strand, within BT0508 at 630.348 kb on - strand, within BT0508 at 630.537 kb on - strand, within BT0508 at 630.621 kb on - strand, within BT0508 at 630.656 kb on - strand, within BT0508 at 630.737 kb on - strand, within BT0508 at 631.257 kb on - strand, within BT0508 at 631.548 kb on - strand, within BT0508 at 631.588 kb on + strand at 631.615 kb on + strand at 631.657 kb on + strand at 631.658 kb on - strand at 631.662 kb on - strand at 631.662 kb on - strand at 631.681 kb on - strand at 631.683 kb on - strand at 631.848 kb on + strand at 631.922 kb on - strand, within BT0509 at 631.936 kb on + strand, within BT0509 at 631.994 kb on + strand, within BT0509 at 632.042 kb on - strand, within BT0509 at 632.110 kb on + strand, within BT0509 at 632.131 kb on - strand, within BT0509 at 632.232 kb on + strand, within BT0509 at 632.416 kb on + strand, within BT0509 at 632.417 kb on - strand, within BT0509 at 632.642 kb on + strand, within BT0509 at 632.665 kb on + strand, within BT0509 at 632.672 kb on - strand, within BT0509 at 632.672 kb on - strand, within BT0509
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Geneticin G418 0.2 mM remove 628,959 + -2.4 628,961 + -0.8 629,046 - -0.8 629,135 + -4.0 629,136 - +0.2 629,139 - -1.4 629,140 + -1.4 629,140 + +0.6 629,141 - +1.5 629,164 + -2.8 629,172 + -3.4 629,308 + BT0507 0.12 +0.6 629,309 - BT0507 0.12 -1.2 629,309 - BT0507 0.12 +0.2 629,350 + BT0507 0.19 -0.8 629,350 + BT0507 0.19 -0.6 629,442 + BT0507 0.34 +0.2 629,442 + BT0507 0.34 +0.8 629,442 + BT0507 0.34 -0.3 629,443 - BT0507 0.34 -0.5 629,443 - BT0507 0.34 -0.8 629,444 + BT0507 0.34 -0.8 629,446 + BT0507 0.35 +0.2 629,447 - BT0507 0.35 -0.1 629,473 - BT0507 0.39 -1.8 629,558 - BT0507 0.53 +0.5 629,629 + BT0507 0.65 -2.6 629,633 - BT0507 0.66 -1.8 629,796 - -3.0 629,796 - -1.8 629,839 - +0.2 629,900 - -0.9 630,091 + BT0508 0.10 -1.4 630,116 + BT0508 0.12 +0.2 630,172 - BT0508 0.15 +0.2 630,173 - BT0508 0.15 -2.6 630,348 - BT0508 0.24 -1.4 630,537 - BT0508 0.35 -2.4 630,621 - BT0508 0.39 -2.1 630,656 - BT0508 0.41 -1.8 630,737 - BT0508 0.46 +0.2 631,257 - BT0508 0.74 -0.8 631,548 - BT0508 0.90 -2.4 631,588 + -1.4 631,615 + +1.5 631,657 + -1.4 631,658 - +1.0 631,662 - +0.2 631,662 - +0.2 631,681 - -0.8 631,683 - +0.5 631,848 + -1.8 631,922 - BT0509 0.11 -0.8 631,936 + BT0509 0.12 +1.8 631,994 + BT0509 0.15 -0.8 632,042 - BT0509 0.18 -1.4 632,110 + BT0509 0.22 -0.8 632,131 - BT0509 0.23 -1.8 632,232 + BT0509 0.29 -1.6 632,416 + BT0509 0.40 -1.8 632,417 - BT0509 0.40 -0.4 632,642 + BT0509 0.53 -0.3 632,665 + BT0509 0.54 -1.2 632,672 - BT0509 0.54 -1.1 632,672 - BT0509 0.54 -0.8
Or see this region's nucleotide sequence