Experiment: MoLS4 with L-Valine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ribB and DVU1776 are separated by 251 nucleotides DVU1776 and cynT are separated by 108 nucleotides cynT and DVU1778 overlap by 71 nucleotides
DVU1775: ribB - 3,4-dihydroxy-2-butanone 4-phosphate synthase (TIGR), at 1,839,892 to 1,840,536
ribB
DVU1776: DVU1776 - hypothetical protein (TIGR), at 1,840,788 to 1,840,901
DVU1776
DVU1777: cynT - carbonic anhydrase (TIGR), at 1,841,010 to 1,841,747
cynT
DVU1778: DVU1778 - cation efflux family protein (TIGR), at 1,841,677 to 1,843,122
DVU1778
Position (kb)
1841
1842 Strain fitness (log2 ratio)
-2
-1
0
1 at 1840.034 kb on + strand, within ribB at 1840.034 kb on + strand, within ribB at 1840.035 kb on + strand, within ribB at 1840.042 kb on - strand, within ribB at 1840.453 kb on + strand, within ribB at 1840.453 kb on + strand, within ribB at 1840.461 kb on - strand, within ribB at 1840.461 kb on - strand, within ribB at 1840.513 kb on - strand at 1840.513 kb on - strand at 1840.640 kb on + strand at 1840.672 kb on - strand at 1840.676 kb on + strand at 1840.684 kb on - strand at 1841.165 kb on - strand, within cynT at 1841.634 kb on + strand, within cynT at 1841.739 kb on + strand at 1841.739 kb on + strand at 1841.856 kb on + strand, within DVU1778 at 1841.907 kb on + strand, within DVU1778 at 1841.925 kb on - strand, within DVU1778 at 1842.059 kb on + strand, within DVU1778 at 1842.059 kb on + strand, within DVU1778 at 1842.067 kb on - strand, within DVU1778 at 1842.113 kb on - strand, within DVU1778 at 1842.116 kb on - strand, within DVU1778 at 1842.152 kb on - strand, within DVU1778 at 1842.170 kb on - strand, within DVU1778 at 1842.203 kb on + strand, within DVU1778 at 1842.212 kb on + strand, within DVU1778 at 1842.304 kb on + strand, within DVU1778 at 1842.429 kb on + strand, within DVU1778 at 1842.541 kb on - strand, within DVU1778 at 1842.582 kb on - strand, within DVU1778 at 1842.601 kb on - strand, within DVU1778 at 1842.625 kb on - strand, within DVU1778
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Valine as nutrient 10mM remove 1,840,034 + ribB DVU1775 0.22 +0.8 1,840,034 + ribB DVU1775 0.22 +0.3 1,840,035 + ribB DVU1775 0.22 -0.2 1,840,042 - ribB DVU1775 0.23 -0.2 1,840,453 + ribB DVU1775 0.87 -0.6 1,840,453 + ribB DVU1775 0.87 -0.4 1,840,461 - ribB DVU1775 0.88 -0.8 1,840,461 - ribB DVU1775 0.88 +0.5 1,840,513 - +0.6 1,840,513 - -1.6 1,840,640 + -0.2 1,840,672 - +0.0 1,840,676 + -1.0 1,840,684 - -0.4 1,841,165 - cynT DVU1777 0.21 +0.4 1,841,634 + cynT DVU1777 0.85 -0.2 1,841,739 + +1.0 1,841,739 + +0.1 1,841,856 + DVU1778 0.12 +0.1 1,841,907 + DVU1778 0.16 -0.9 1,841,925 - DVU1778 0.17 +0.3 1,842,059 + DVU1778 0.26 -0.1 1,842,059 + DVU1778 0.26 -0.8 1,842,067 - DVU1778 0.27 -0.7 1,842,113 - DVU1778 0.30 -0.7 1,842,116 - DVU1778 0.30 -1.3 1,842,152 - DVU1778 0.33 -0.4 1,842,170 - DVU1778 0.34 -0.0 1,842,203 + DVU1778 0.36 +0.3 1,842,212 + DVU1778 0.37 -2.2 1,842,304 + DVU1778 0.43 -0.4 1,842,429 + DVU1778 0.52 +0.5 1,842,541 - DVU1778 0.60 -0.3 1,842,582 - DVU1778 0.63 +0.1 1,842,601 - DVU1778 0.64 -1.3 1,842,625 - DVU1778 0.66 +0.1
Or see this region's nucleotide sequence