Experiment: MoLS4 with L-Valine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppiB-1 and hisH are separated by 187 nucleotides hisH and hisF overlap by 11 nucleotides hisF and DORF26924 are separated by 82 nucleotides
DVU0284: ppiB-1 - peptidyl-prolyl cis-trans isomerase B (TIGR), at 330,771 to 331,277
ppiB-1
DVU0285: hisH - IGP synthase, amidotransferase subunit (EC 4.3.2.10) <small>(from data)</small>, at 331,465 to 332,106
hisH
DVU0286: hisF - imidazoleglycerol phosphate synthase, cyclase subunit (TIGR), at 332,096 to 332,875
hisF
DORF26924: DORF26924 - no description, at 332,958 to 333,728
DORF26924
Position (kb) 332
333 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 331.109 kb on - strand, within ppiB-1 at 331.110 kb on - strand, within ppiB-1 at 331.125 kb on + strand, within ppiB-1 at 331.131 kb on + strand, within ppiB-1 at 331.133 kb on - strand, within ppiB-1 at 331.133 kb on - strand, within ppiB-1 at 331.196 kb on + strand, within ppiB-1 at 331.229 kb on - strand at 331.239 kb on - strand at 331.257 kb on + strand at 331.277 kb on + strand at 331.277 kb on + strand at 331.284 kb on + strand at 331.284 kb on + strand at 331.353 kb on + strand at 331.417 kb on + strand at 331.495 kb on + strand at 331.592 kb on + strand, within hisH at 331.640 kb on - strand, within hisH at 331.655 kb on - strand, within hisH at 331.724 kb on + strand, within hisH at 331.852 kb on + strand, within hisH at 331.852 kb on + strand, within hisH at 331.852 kb on + strand, within hisH at 331.910 kb on - strand, within hisH at 331.910 kb on - strand, within hisH at 331.933 kb on - strand, within hisH at 331.980 kb on - strand, within hisH at 331.985 kb on - strand, within hisH at 331.995 kb on - strand, within hisH at 331.997 kb on + strand, within hisH at 332.004 kb on + strand, within hisH at 332.019 kb on + strand, within hisH at 332.056 kb on - strand at 332.121 kb on + strand at 332.129 kb on - strand at 332.129 kb on - strand at 332.179 kb on - strand, within hisF at 332.219 kb on - strand, within hisF at 332.271 kb on + strand, within hisF at 332.387 kb on + strand, within hisF at 332.458 kb on - strand, within hisF at 332.495 kb on + strand, within hisF at 332.554 kb on + strand, within hisF at 332.609 kb on + strand, within hisF at 332.615 kb on - strand, within hisF at 332.740 kb on + strand, within hisF at 332.755 kb on + strand, within hisF at 333.775 kb on + strand at 333.862 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Valine as nutrient 10mM remove 331,109 - ppiB-1 DVU0284 0.67 -0.2 331,110 - ppiB-1 DVU0284 0.67 -0.3 331,125 + ppiB-1 DVU0284 0.70 -0.2 331,131 + ppiB-1 DVU0284 0.71 +0.0 331,133 - ppiB-1 DVU0284 0.71 -0.1 331,133 - ppiB-1 DVU0284 0.71 -0.5 331,196 + ppiB-1 DVU0284 0.84 -0.3 331,229 - -2.1 331,239 - -1.3 331,257 + -1.1 331,277 + +0.6 331,277 + -0.5 331,284 + -0.5 331,284 + -0.2 331,353 + +0.1 331,417 + -0.8 331,495 + -1.5 331,592 + hisH DVU0285 0.20 -1.1 331,640 - hisH DVU0285 0.27 -1.5 331,655 - hisH DVU0285 0.30 -3.3 331,724 + hisH DVU0285 0.40 -3.7 331,852 + hisH DVU0285 0.60 -2.0 331,852 + hisH DVU0285 0.60 -2.5 331,852 + hisH DVU0285 0.60 -3.5 331,910 - hisH DVU0285 0.69 -0.2 331,910 - hisH DVU0285 0.69 -1.5 331,933 - hisH DVU0285 0.73 -2.4 331,980 - hisH DVU0285 0.80 -4.3 331,985 - hisH DVU0285 0.81 -2.2 331,995 - hisH DVU0285 0.83 -1.1 331,997 + hisH DVU0285 0.83 -2.4 332,004 + hisH DVU0285 0.84 -4.9 332,019 + hisH DVU0285 0.86 -2.9 332,056 - -1.7 332,121 + -1.8 332,129 - -0.9 332,129 - -3.8 332,179 - hisF DVU0286 0.11 -1.9 332,219 - hisF DVU0286 0.16 -2.5 332,271 + hisF DVU0286 0.22 -1.2 332,387 + hisF DVU0286 0.37 -1.9 332,458 - hisF DVU0286 0.46 -2.3 332,495 + hisF DVU0286 0.51 -0.6 332,554 + hisF DVU0286 0.59 -1.2 332,609 + hisF DVU0286 0.66 -0.7 332,615 - hisF DVU0286 0.67 -0.5 332,740 + hisF DVU0286 0.83 -2.2 332,755 + hisF DVU0286 0.84 -3.6 333,775 + +0.1 333,862 + -1.4
Or see this region's nucleotide sequence