Experiment: MoLS4 with L-Valine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2331 and proC are separated by 330 nucleotides proC and ndk overlap by 1 nucleotides ndk and DVU2335 are separated by 551 nucleotides
DVU2331: DVU2331 - Smr family protein (TIGR), at 2,427,033 to 2,428,262
DVU2331
DVU2332: proC - pyrroline-5-carboxylate reductase (EC 1.5.1.2) (from data) , at 2,428,593 to 2,429,390
proC
DVU2333: ndk - nucleoside diphosphate kinase (TIGR), at 2,429,390 to 2,429,809
ndk
DVU2335: DVU2335 - conserved domain protein (TIGR), at 2,430,361 to 2,431,911
DVU2335
Position (kb)
2428
2429
2430 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2427.703 kb on - strand, within DVU2331 at 2427.799 kb on + strand, within DVU2331 at 2427.804 kb on + strand, within DVU2331 at 2427.812 kb on - strand, within DVU2331 at 2427.814 kb on + strand, within DVU2331 at 2427.915 kb on + strand, within DVU2331 at 2427.915 kb on + strand, within DVU2331 at 2427.950 kb on - strand, within DVU2331 at 2428.366 kb on - strand at 2428.467 kb on + strand at 2428.826 kb on - strand, within proC at 2428.842 kb on - strand, within proC at 2428.933 kb on + strand, within proC at 2429.115 kb on + strand, within proC at 2429.612 kb on + strand, within ndk at 2429.658 kb on + strand, within ndk at 2429.666 kb on - strand, within ndk at 2429.666 kb on - strand, within ndk at 2429.706 kb on + strand, within ndk at 2429.712 kb on + strand, within ndk at 2429.859 kb on - strand at 2429.923 kb on + strand at 2430.000 kb on - strand at 2430.069 kb on - strand at 2430.072 kb on + strand at 2430.180 kb on + strand at 2430.227 kb on - strand at 2430.268 kb on - strand at 2430.297 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Valine as nutrient 10mM remove 2,427,703 - DVU2331 0.54 -0.3 2,427,799 + DVU2331 0.62 -0.3 2,427,804 + DVU2331 0.63 -0.3 2,427,812 - DVU2331 0.63 -0.2 2,427,814 + DVU2331 0.63 -0.0 2,427,915 + DVU2331 0.72 +3.8 2,427,915 + DVU2331 0.72 -0.0 2,427,950 - DVU2331 0.75 -1.7 2,428,366 - +0.2 2,428,467 + -0.5 2,428,826 - proC DVU2332 0.29 -2.1 2,428,842 - proC DVU2332 0.31 -1.7 2,428,933 + proC DVU2332 0.43 -3.8 2,429,115 + proC DVU2332 0.65 -3.5 2,429,612 + ndk DVU2333 0.53 -1.0 2,429,658 + ndk DVU2333 0.64 -0.0 2,429,666 - ndk DVU2333 0.66 +0.2 2,429,666 - ndk DVU2333 0.66 -0.2 2,429,706 + ndk DVU2333 0.75 +0.2 2,429,712 + ndk DVU2333 0.77 -0.2 2,429,859 - -0.9 2,429,923 + -0.1 2,430,000 - +0.2 2,430,069 - +0.2 2,430,072 + -1.0 2,430,180 + -1.4 2,430,227 - -0.0 2,430,268 - +2.2 2,430,297 + -0.3
Or see this region's nucleotide sequence