Experiment: KB with Dimethyl Sulfoxide 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2940 and Psyr_2941 are separated by 496 nucleotides Psyr_2941 and Psyr_2942 overlap by 4 nucleotides Psyr_2942 and Psyr_2943 overlap by 4 nucleotides
Psyr_2940: Psyr_2940 - PAS, at 3,527,685 to 3,531,746
_2940
Psyr_2941: Psyr_2941 - amino acid ABC transporter substrate-binding protein, PAAT family, at 3,532,243 to 3,533,115
_2941
Psyr_2942: Psyr_2942 - amino acid ABC transporter ATP-binding protein, PAAT family, at 3,533,112 to 3,533,873
_2942
Psyr_2943: Psyr_2943 - amino acid ABC transporter membrane protein 2, PAAT family, at 3,533,870 to 3,534,529
_2943
Position (kb)
3532
3533
3534 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3531.252 kb on - strand, within Psyr_2940 at 3531.391 kb on - strand at 3531.509 kb on + strand at 3531.510 kb on - strand at 3531.510 kb on - strand at 3531.565 kb on + strand at 3531.565 kb on + strand at 3531.585 kb on + strand at 3531.585 kb on + strand at 3531.585 kb on + strand at 3531.585 kb on + strand at 3531.586 kb on - strand at 3531.586 kb on - strand at 3531.586 kb on - strand at 3531.586 kb on - strand at 3531.631 kb on - strand at 3531.837 kb on + strand at 3531.838 kb on - strand at 3531.956 kb on + strand at 3531.957 kb on - strand at 3531.963 kb on + strand at 3531.963 kb on + strand at 3532.042 kb on - strand at 3532.042 kb on - strand at 3532.042 kb on - strand at 3532.042 kb on - strand at 3532.046 kb on - strand at 3532.074 kb on + strand at 3532.142 kb on + strand at 3532.143 kb on - strand at 3532.312 kb on + strand at 3532.312 kb on + strand at 3532.312 kb on + strand at 3532.312 kb on + strand at 3532.312 kb on + strand at 3532.312 kb on + strand at 3532.312 kb on + strand at 3532.313 kb on - strand at 3532.313 kb on - strand at 3532.313 kb on - strand at 3532.313 kb on - strand at 3532.316 kb on + strand at 3532.316 kb on + strand at 3532.316 kb on + strand at 3532.316 kb on + strand at 3532.316 kb on + strand at 3532.316 kb on + strand at 3532.316 kb on + strand at 3532.317 kb on - strand at 3532.317 kb on - strand at 3532.317 kb on - strand at 3532.325 kb on + strand at 3532.326 kb on - strand at 3532.340 kb on + strand, within Psyr_2941 at 3532.341 kb on - strand, within Psyr_2941 at 3532.341 kb on - strand, within Psyr_2941 at 3532.512 kb on - strand, within Psyr_2941 at 3532.609 kb on - strand, within Psyr_2941 at 3532.784 kb on - strand, within Psyr_2941 at 3532.784 kb on - strand, within Psyr_2941 at 3532.784 kb on - strand, within Psyr_2941 at 3532.784 kb on - strand, within Psyr_2941 at 3532.784 kb on - strand, within Psyr_2941 at 3532.808 kb on - strand, within Psyr_2941 at 3532.908 kb on - strand, within Psyr_2941 at 3532.997 kb on + strand, within Psyr_2941 at 3533.089 kb on + strand at 3533.093 kb on - strand at 3533.093 kb on - strand at 3533.093 kb on - strand at 3533.147 kb on - strand at 3533.586 kb on + strand, within Psyr_2942 at 3533.611 kb on + strand, within Psyr_2942 at 3533.611 kb on + strand, within Psyr_2942 at 3533.611 kb on + strand, within Psyr_2942 at 3533.612 kb on - strand, within Psyr_2942 at 3533.612 kb on - strand, within Psyr_2942 at 3533.612 kb on - strand, within Psyr_2942 at 3533.932 kb on + strand at 3533.958 kb on + strand, within Psyr_2943 at 3533.959 kb on - strand, within Psyr_2943 at 3533.977 kb on - strand, within Psyr_2943 at 3534.067 kb on - strand, within Psyr_2943 at 3534.067 kb on - strand, within Psyr_2943 at 3534.067 kb on - strand, within Psyr_2943 at 3534.067 kb on - strand, within Psyr_2943 at 3534.114 kb on - strand, within Psyr_2943
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Dimethyl Sulfoxide 1.25 vol% remove 3,531,252 - Psyr_2940 0.88 -1.5 3,531,391 - +1.2 3,531,509 + -0.2 3,531,510 - +0.7 3,531,510 - -0.7 3,531,565 + +1.0 3,531,565 + -2.0 3,531,585 + -0.4 3,531,585 + -2.0 3,531,585 + +1.2 3,531,585 + -0.9 3,531,586 - +0.2 3,531,586 - -0.1 3,531,586 - -1.5 3,531,586 - -2.5 3,531,631 - +1.3 3,531,837 + +1.4 3,531,838 - +0.1 3,531,956 + +0.3 3,531,957 - -2.8 3,531,963 + +1.2 3,531,963 + +1.0 3,532,042 - +1.2 3,532,042 - -0.0 3,532,042 - -1.3 3,532,042 - -1.1 3,532,046 - -0.1 3,532,074 + -0.3 3,532,142 + -1.0 3,532,143 - -0.2 3,532,312 + -1.2 3,532,312 + +1.4 3,532,312 + -1.2 3,532,312 + -1.1 3,532,312 + -0.2 3,532,312 + -1.1 3,532,312 + +0.6 3,532,313 - +0.5 3,532,313 - -0.9 3,532,313 - +0.2 3,532,313 - +3.3 3,532,316 + -0.2 3,532,316 + +0.2 3,532,316 + -0.7 3,532,316 + -0.5 3,532,316 + -0.3 3,532,316 + +3.0 3,532,316 + -0.4 3,532,317 - -1.0 3,532,317 - +1.2 3,532,317 - -0.1 3,532,325 + +0.6 3,532,326 - +0.6 3,532,340 + Psyr_2941 0.11 +1.5 3,532,341 - Psyr_2941 0.11 +0.1 3,532,341 - Psyr_2941 0.11 -0.6 3,532,512 - Psyr_2941 0.31 +2.8 3,532,609 - Psyr_2941 0.42 +0.1 3,532,784 - Psyr_2941 0.62 +0.0 3,532,784 - Psyr_2941 0.62 +1.0 3,532,784 - Psyr_2941 0.62 +0.3 3,532,784 - Psyr_2941 0.62 +0.3 3,532,784 - Psyr_2941 0.62 -0.2 3,532,808 - Psyr_2941 0.65 -0.4 3,532,908 - Psyr_2941 0.76 +3.5 3,532,997 + Psyr_2941 0.86 +1.2 3,533,089 + -0.7 3,533,093 - +0.5 3,533,093 - -0.2 3,533,093 - +2.8 3,533,147 - -1.5 3,533,586 + Psyr_2942 0.62 -0.9 3,533,611 + Psyr_2942 0.65 +0.2 3,533,611 + Psyr_2942 0.65 -0.7 3,533,611 + Psyr_2942 0.65 -0.2 3,533,612 - Psyr_2942 0.66 +0.8 3,533,612 - Psyr_2942 0.66 -0.7 3,533,612 - Psyr_2942 0.66 -0.2 3,533,932 + -2.1 3,533,958 + Psyr_2943 0.13 -0.3 3,533,959 - Psyr_2943 0.13 +0.4 3,533,977 - Psyr_2943 0.16 +1.3 3,534,067 - Psyr_2943 0.30 -0.5 3,534,067 - Psyr_2943 0.30 +1.1 3,534,067 - Psyr_2943 0.30 -3.9 3,534,067 - Psyr_2943 0.30 +1.4 3,534,114 - Psyr_2943 0.37 +0.6
Or see this region's nucleotide sequence