Experiment: KB with Dimethyl Sulfoxide 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0409 and Psyr_0410 are separated by 10 nucleotides Psyr_0410 and Psyr_0411 are separated by 358 nucleotides
Psyr_0409: Psyr_0409 - 3-dehydroquinate synthase, at 439,198 to 440,301
_0409
Psyr_0410: Psyr_0410 - conserved hypothetical protein, at 440,312 to 441,934
_0410
Psyr_0411: Psyr_0411 - glutamate synthase (NADPH) large subunit, at 442,293 to 446,738
_0411
Position (kb)
440
441
442 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 440.317 kb on + strand at 440.317 kb on + strand at 440.317 kb on + strand at 440.317 kb on + strand at 440.317 kb on + strand at 440.317 kb on + strand at 440.317 kb on + strand at 440.317 kb on + strand at 440.318 kb on - strand at 440.318 kb on - strand at 440.318 kb on - strand at 440.318 kb on - strand at 440.318 kb on - strand at 440.318 kb on - strand at 440.693 kb on + strand, within Psyr_0410 at 440.787 kb on + strand, within Psyr_0410 at 440.787 kb on + strand, within Psyr_0410 at 440.787 kb on + strand, within Psyr_0410 at 440.787 kb on + strand, within Psyr_0410 at 440.787 kb on + strand, within Psyr_0410 at 440.787 kb on + strand, within Psyr_0410 at 440.787 kb on + strand, within Psyr_0410 at 440.787 kb on + strand, within Psyr_0410 at 440.787 kb on + strand, within Psyr_0410 at 440.788 kb on - strand, within Psyr_0410 at 440.788 kb on - strand, within Psyr_0410 at 440.788 kb on - strand, within Psyr_0410 at 440.788 kb on - strand, within Psyr_0410 at 440.788 kb on - strand, within Psyr_0410 at 440.788 kb on - strand, within Psyr_0410 at 440.788 kb on - strand, within Psyr_0410 at 440.788 kb on - strand, within Psyr_0410 at 440.788 kb on - strand, within Psyr_0410 at 440.894 kb on + strand, within Psyr_0410 at 440.894 kb on + strand, within Psyr_0410 at 440.895 kb on - strand, within Psyr_0410 at 440.918 kb on + strand, within Psyr_0410 at 440.918 kb on + strand, within Psyr_0410 at 440.918 kb on + strand, within Psyr_0410 at 440.918 kb on + strand, within Psyr_0410 at 440.919 kb on - strand, within Psyr_0410 at 441.031 kb on - strand, within Psyr_0410 at 441.031 kb on - strand, within Psyr_0410 at 441.031 kb on - strand, within Psyr_0410 at 441.120 kb on + strand, within Psyr_0410 at 441.120 kb on + strand, within Psyr_0410 at 441.120 kb on + strand, within Psyr_0410 at 441.121 kb on - strand, within Psyr_0410 at 441.121 kb on - strand, within Psyr_0410 at 441.121 kb on - strand, within Psyr_0410 at 441.144 kb on + strand, within Psyr_0410 at 441.144 kb on + strand, within Psyr_0410 at 441.145 kb on - strand, within Psyr_0410 at 441.445 kb on + strand, within Psyr_0410 at 441.446 kb on - strand, within Psyr_0410 at 441.446 kb on - strand, within Psyr_0410 at 441.683 kb on + strand, within Psyr_0410 at 441.684 kb on - strand, within Psyr_0410 at 441.684 kb on - strand, within Psyr_0410 at 441.684 kb on - strand, within Psyr_0410 at 441.731 kb on + strand, within Psyr_0410 at 441.731 kb on + strand, within Psyr_0410 at 441.731 kb on + strand, within Psyr_0410 at 441.731 kb on + strand, within Psyr_0410 at 441.731 kb on + strand, within Psyr_0410 at 441.731 kb on + strand, within Psyr_0410 at 441.732 kb on - strand, within Psyr_0410 at 441.732 kb on - strand, within Psyr_0410 at 441.762 kb on - strand, within Psyr_0410 at 441.762 kb on - strand, within Psyr_0410 at 441.762 kb on - strand, within Psyr_0410 at 441.794 kb on + strand at 441.794 kb on + strand at 441.794 kb on + strand at 441.794 kb on + strand at 441.794 kb on + strand at 441.794 kb on + strand at 441.794 kb on + strand at 441.794 kb on + strand at 441.794 kb on + strand at 441.794 kb on + strand at 441.794 kb on + strand at 441.794 kb on + strand at 441.794 kb on + strand at 441.794 kb on + strand at 441.795 kb on - strand at 441.795 kb on - strand at 441.795 kb on - strand at 441.795 kb on - strand at 441.795 kb on - strand at 441.795 kb on - strand at 441.795 kb on - strand at 441.795 kb on - strand at 441.806 kb on + strand at 441.806 kb on + strand at 441.852 kb on + strand at 441.892 kb on + strand at 442.031 kb on - strand at 442.031 kb on - strand at 442.042 kb on - strand at 442.079 kb on + strand at 442.178 kb on + strand at 442.178 kb on + strand at 442.178 kb on + strand at 442.178 kb on + strand at 442.179 kb on - strand at 442.179 kb on - strand at 442.292 kb on + strand at 442.292 kb on + strand at 442.293 kb on - strand at 442.308 kb on + strand at 442.331 kb on + strand at 442.331 kb on + strand at 442.331 kb on + strand at 442.332 kb on - strand at 442.358 kb on + strand at 442.358 kb on + strand at 442.358 kb on + strand at 442.359 kb on - strand at 442.359 kb on - strand at 442.359 kb on - strand at 442.545 kb on + strand at 442.546 kb on - strand at 442.723 kb on + strand at 442.723 kb on + strand at 442.723 kb on + strand at 442.723 kb on + strand at 442.724 kb on - strand at 442.824 kb on + strand, within Psyr_0411 at 442.824 kb on + strand, within Psyr_0411 at 442.824 kb on + strand, within Psyr_0411 at 442.824 kb on + strand, within Psyr_0411 at 442.824 kb on + strand, within Psyr_0411 at 442.824 kb on + strand, within Psyr_0411 at 442.824 kb on + strand, within Psyr_0411 at 442.824 kb on + strand, within Psyr_0411 at 442.825 kb on - strand, within Psyr_0411 at 442.825 kb on - strand, within Psyr_0411 at 442.825 kb on - strand, within Psyr_0411 at 442.825 kb on - strand, within Psyr_0411 at 442.825 kb on - strand, within Psyr_0411 at 442.825 kb on - strand, within Psyr_0411 at 442.825 kb on - strand, within Psyr_0411 at 442.857 kb on + strand, within Psyr_0411 at 442.857 kb on + strand, within Psyr_0411 at 442.858 kb on - strand, within Psyr_0411 at 442.858 kb on - strand, within Psyr_0411 at 442.858 kb on - strand, within Psyr_0411 at 442.858 kb on - strand, within Psyr_0411 at 442.858 kb on - strand, within Psyr_0411 at 442.858 kb on - strand, within Psyr_0411 at 442.858 kb on - strand, within Psyr_0411 at 442.932 kb on - strand, within Psyr_0411 at 442.932 kb on - strand, within Psyr_0411
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Dimethyl Sulfoxide 1.25 vol% remove 440,317 + -0.2 440,317 + +1.1 440,317 + -2.8 440,317 + -1.1 440,317 + -0.3 440,317 + +0.5 440,317 + -0.2 440,317 + -0.7 440,318 - +0.7 440,318 - -0.7 440,318 - +0.5 440,318 - +0.5 440,318 - -0.4 440,318 - +0.3 440,693 + Psyr_0410 0.23 +2.8 440,787 + Psyr_0410 0.29 -0.6 440,787 + Psyr_0410 0.29 +0.0 440,787 + Psyr_0410 0.29 -2.1 440,787 + Psyr_0410 0.29 -0.7 440,787 + Psyr_0410 0.29 +0.4 440,787 + Psyr_0410 0.29 -0.1 440,787 + Psyr_0410 0.29 -0.1 440,787 + Psyr_0410 0.29 -1.3 440,787 + Psyr_0410 0.29 +0.4 440,788 - Psyr_0410 0.29 +0.4 440,788 - Psyr_0410 0.29 +0.6 440,788 - Psyr_0410 0.29 -1.0 440,788 - Psyr_0410 0.29 -1.6 440,788 - Psyr_0410 0.29 +0.4 440,788 - Psyr_0410 0.29 -1.2 440,788 - Psyr_0410 0.29 -1.9 440,788 - Psyr_0410 0.29 +0.4 440,788 - Psyr_0410 0.29 -0.8 440,894 + Psyr_0410 0.36 -1.2 440,894 + Psyr_0410 0.36 -0.8 440,895 - Psyr_0410 0.36 -0.8 440,918 + Psyr_0410 0.37 -1.5 440,918 + Psyr_0410 0.37 +0.6 440,918 + Psyr_0410 0.37 -0.9 440,918 + Psyr_0410 0.37 -0.7 440,919 - Psyr_0410 0.37 -0.9 441,031 - Psyr_0410 0.44 -1.1 441,031 - Psyr_0410 0.44 +0.4 441,031 - Psyr_0410 0.44 -0.1 441,120 + Psyr_0410 0.50 -1.0 441,120 + Psyr_0410 0.50 -0.4 441,120 + Psyr_0410 0.50 -0.6 441,121 - Psyr_0410 0.50 -1.3 441,121 - Psyr_0410 0.50 +0.2 441,121 - Psyr_0410 0.50 -1.5 441,144 + Psyr_0410 0.51 -1.6 441,144 + Psyr_0410 0.51 -1.0 441,145 - Psyr_0410 0.51 +0.2 441,445 + Psyr_0410 0.70 +0.5 441,446 - Psyr_0410 0.70 -2.1 441,446 - Psyr_0410 0.70 -0.9 441,683 + Psyr_0410 0.84 -1.0 441,684 - Psyr_0410 0.85 -0.4 441,684 - Psyr_0410 0.85 +0.0 441,684 - Psyr_0410 0.85 -0.4 441,731 + Psyr_0410 0.87 -1.6 441,731 + Psyr_0410 0.87 -0.1 441,731 + Psyr_0410 0.87 +0.3 441,731 + Psyr_0410 0.87 +0.6 441,731 + Psyr_0410 0.87 -0.2 441,731 + Psyr_0410 0.87 +1.2 441,732 - Psyr_0410 0.87 -0.4 441,732 - Psyr_0410 0.87 -0.4 441,762 - Psyr_0410 0.89 -2.6 441,762 - Psyr_0410 0.89 +0.8 441,762 - Psyr_0410 0.89 -0.6 441,794 + -2.2 441,794 + -0.9 441,794 + -1.0 441,794 + -1.4 441,794 + -1.4 441,794 + -0.8 441,794 + -0.8 441,794 + -0.1 441,794 + -0.1 441,794 + +0.1 441,794 + -1.6 441,794 + -0.0 441,794 + -1.1 441,794 + +1.1 441,795 - -1.8 441,795 - +0.2 441,795 - -3.2 441,795 - +1.8 441,795 - -2.1 441,795 - -0.2 441,795 - -0.1 441,795 - -0.8 441,806 + +0.1 441,806 + +0.6 441,852 + -1.9 441,892 + +0.4 442,031 - +1.2 442,031 - +0.4 442,042 - -0.1 442,079 + +1.1 442,178 + +2.4 442,178 + +1.2 442,178 + -0.3 442,178 + +1.2 442,179 - +0.7 442,179 - -1.6 442,292 + -1.4 442,292 + -1.1 442,293 - +3.8 442,308 + -1.6 442,331 + -0.5 442,331 + -0.6 442,331 + -2.1 442,332 - -0.4 442,358 + +0.6 442,358 + +0.2 442,358 + -0.9 442,359 - +1.6 442,359 - +0.7 442,359 - -0.6 442,545 + -0.6 442,546 - -0.3 442,723 + -0.1 442,723 + -1.9 442,723 + -0.2 442,723 + +0.1 442,724 - -0.7 442,824 + Psyr_0411 0.12 +0.8 442,824 + Psyr_0411 0.12 -0.7 442,824 + Psyr_0411 0.12 +1.5 442,824 + Psyr_0411 0.12 -0.5 442,824 + Psyr_0411 0.12 -0.6 442,824 + Psyr_0411 0.12 -0.8 442,824 + Psyr_0411 0.12 -0.6 442,824 + Psyr_0411 0.12 +0.6 442,825 - Psyr_0411 0.12 +0.3 442,825 - Psyr_0411 0.12 +0.4 442,825 - Psyr_0411 0.12 -3.4 442,825 - Psyr_0411 0.12 -0.2 442,825 - Psyr_0411 0.12 +0.8 442,825 - Psyr_0411 0.12 -0.1 442,825 - Psyr_0411 0.12 +3.2 442,857 + Psyr_0411 0.13 +0.8 442,857 + Psyr_0411 0.13 -0.6 442,858 - Psyr_0411 0.13 -0.2 442,858 - Psyr_0411 0.13 +0.8 442,858 - Psyr_0411 0.13 +1.6 442,858 - Psyr_0411 0.13 -0.4 442,858 - Psyr_0411 0.13 +3.2 442,858 - Psyr_0411 0.13 -0.2 442,858 - Psyr_0411 0.13 -0.5 442,932 - Psyr_0411 0.14 -1.2 442,932 - Psyr_0411 0.14 -0.8
Or see this region's nucleotide sequence