Experiment: R2A with Tricalcium phosphate 0.5 g/L
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_004547 and ABIE53_004548 are separated by 21 nucleotides ABIE53_004548 and ABIE53_004549 are separated by 77 nucleotides ABIE53_004549 and ABIE53_004550 are separated by 4 nucleotides ABIE53_004550 and ABIE53_004551 overlap by 4 nucleotides
ABIE53_004547: ABIE53_004547 - 3-phenylpropionate/cinnamic acid dioxygenase small subunit, at 736,482 to 736,982
_004547
ABIE53_004548: ABIE53_004548 - 4-oxalocrotonate tautomerase, at 737,004 to 737,411
_004548
ABIE53_004549: ABIE53_004549 - hypothetical protein, at 737,489 to 737,845
_004549
ABIE53_004550: ABIE53_004550 - hypothetical protein, at 737,850 to 738,365
_004550
ABIE53_004551: ABIE53_004551 - amidase, at 738,362 to 739,498
_004551
Position (kb) 737
738
739 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 737.056 kb on - strand, within ABIE53_004548 at 737.161 kb on + strand, within ABIE53_004548 at 737.162 kb on - strand, within ABIE53_004548 at 737.485 kb on - strand at 737.559 kb on + strand, within ABIE53_004549 at 737.559 kb on + strand, within ABIE53_004549 at 737.559 kb on + strand, within ABIE53_004549 at 737.559 kb on + strand, within ABIE53_004549 at 737.559 kb on + strand, within ABIE53_004549 at 737.559 kb on + strand, within ABIE53_004549 at 737.560 kb on - strand, within ABIE53_004549 at 737.560 kb on - strand, within ABIE53_004549 at 737.681 kb on + strand, within ABIE53_004549 at 737.681 kb on + strand, within ABIE53_004549 at 737.681 kb on + strand, within ABIE53_004549 at 737.681 kb on + strand, within ABIE53_004549 at 737.681 kb on + strand, within ABIE53_004549 at 737.681 kb on + strand, within ABIE53_004549 at 737.682 kb on - strand, within ABIE53_004549 at 737.682 kb on - strand, within ABIE53_004549 at 737.796 kb on + strand, within ABIE53_004549 at 737.839 kb on + strand at 737.839 kb on + strand at 737.841 kb on + strand at 737.841 kb on + strand at 737.841 kb on + strand at 737.842 kb on - strand at 737.842 kb on - strand at 737.842 kb on - strand at 737.934 kb on + strand, within ABIE53_004550 at 737.934 kb on + strand, within ABIE53_004550 at 737.934 kb on + strand, within ABIE53_004550 at 737.934 kb on + strand, within ABIE53_004550 at 737.992 kb on + strand, within ABIE53_004550 at 738.000 kb on + strand, within ABIE53_004550 at 738.000 kb on + strand, within ABIE53_004550 at 738.000 kb on + strand, within ABIE53_004550 at 738.094 kb on - strand, within ABIE53_004550 at 738.094 kb on - strand, within ABIE53_004550 at 738.149 kb on + strand, within ABIE53_004550 at 738.157 kb on + strand, within ABIE53_004550 at 738.168 kb on - strand, within ABIE53_004550 at 738.208 kb on - strand, within ABIE53_004550 at 738.208 kb on - strand, within ABIE53_004550 at 738.281 kb on + strand, within ABIE53_004550 at 738.941 kb on - strand, within ABIE53_004551 at 739.202 kb on - strand, within ABIE53_004551 at 739.259 kb on - strand, within ABIE53_004551
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Tricalcium phosphate 0.5 g/L remove 737,056 - ABIE53_004548 0.13 -0.2 737,161 + ABIE53_004548 0.38 +0.1 737,162 - ABIE53_004548 0.39 -2.5 737,485 - +0.3 737,559 + ABIE53_004549 0.20 -1.1 737,559 + ABIE53_004549 0.20 -1.0 737,559 + ABIE53_004549 0.20 -0.2 737,559 + ABIE53_004549 0.20 -1.8 737,559 + ABIE53_004549 0.20 -0.5 737,559 + ABIE53_004549 0.20 -1.3 737,560 - ABIE53_004549 0.20 +2.0 737,560 - ABIE53_004549 0.20 +0.0 737,681 + ABIE53_004549 0.54 +1.9 737,681 + ABIE53_004549 0.54 +0.7 737,681 + ABIE53_004549 0.54 +1.4 737,681 + ABIE53_004549 0.54 +0.4 737,681 + ABIE53_004549 0.54 +2.7 737,681 + ABIE53_004549 0.54 -1.0 737,682 - ABIE53_004549 0.54 -1.3 737,682 - ABIE53_004549 0.54 -1.4 737,796 + ABIE53_004549 0.86 -0.3 737,839 + +0.5 737,839 + -0.5 737,841 + -3.0 737,841 + -0.3 737,841 + +1.7 737,842 - -0.4 737,842 - +0.7 737,842 - -2.7 737,934 + ABIE53_004550 0.16 +2.0 737,934 + ABIE53_004550 0.16 +2.7 737,934 + ABIE53_004550 0.16 +2.0 737,934 + ABIE53_004550 0.16 -2.8 737,992 + ABIE53_004550 0.28 -2.1 738,000 + ABIE53_004550 0.29 +1.3 738,000 + ABIE53_004550 0.29 +1.1 738,000 + ABIE53_004550 0.29 -0.1 738,094 - ABIE53_004550 0.47 +0.1 738,094 - ABIE53_004550 0.47 -0.1 738,149 + ABIE53_004550 0.58 +1.1 738,157 + ABIE53_004550 0.59 +0.1 738,168 - ABIE53_004550 0.62 -0.3 738,208 - ABIE53_004550 0.69 +0.0 738,208 - ABIE53_004550 0.69 -0.5 738,281 + ABIE53_004550 0.84 -0.8 738,941 - ABIE53_004551 0.51 +1.7 739,202 - ABIE53_004551 0.74 +1.7 739,259 - ABIE53_004551 0.79 -0.4
Or see this region's nucleotide sequence