Experiment: R2A with Tricalcium phosphate 0.5 g/L
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_004128 and ABIE53_004129 are separated by 56 nucleotides ABIE53_004129 and ABIE53_004130 are separated by 16 nucleotides ABIE53_004130 and ABIE53_004131 overlap by 4 nucleotides
ABIE53_004128: ABIE53_004128 - HAD superfamily hydrolase (TIGR01509 family), at 299,865 to 300,539
_004128
ABIE53_004129: ABIE53_004129 - hypothetical protein, at 300,596 to 301,243
_004129
ABIE53_004130: ABIE53_004130 - flagellar motor protein MotB, at 301,260 to 301,916
_004130
ABIE53_004131: ABIE53_004131 - polyhydroxyalkanoate synthesis regulator phasin, at 301,913 to 304,477
_004131
Position (kb) 301
302 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 300.276 kb on + strand, within ABIE53_004128 at 300.276 kb on + strand, within ABIE53_004128 at 300.276 kb on + strand, within ABIE53_004128 at 300.276 kb on + strand, within ABIE53_004128 at 300.276 kb on + strand, within ABIE53_004128 at 300.276 kb on + strand, within ABIE53_004128 at 300.276 kb on + strand, within ABIE53_004128 at 300.276 kb on + strand, within ABIE53_004128 at 300.276 kb on + strand, within ABIE53_004128 at 300.276 kb on + strand, within ABIE53_004128 at 300.276 kb on + strand, within ABIE53_004128 at 300.277 kb on - strand, within ABIE53_004128 at 300.277 kb on - strand, within ABIE53_004128 at 300.277 kb on - strand, within ABIE53_004128 at 300.277 kb on - strand, within ABIE53_004128 at 300.277 kb on - strand, within ABIE53_004128 at 300.277 kb on - strand, within ABIE53_004128 at 300.296 kb on + strand, within ABIE53_004128 at 300.296 kb on + strand, within ABIE53_004128 at 300.296 kb on + strand, within ABIE53_004128 at 300.297 kb on - strand, within ABIE53_004128 at 300.297 kb on - strand, within ABIE53_004128 at 300.297 kb on - strand, within ABIE53_004128 at 300.297 kb on - strand, within ABIE53_004128 at 300.481 kb on + strand at 300.481 kb on + strand at 300.481 kb on + strand at 300.482 kb on - strand at 300.482 kb on - strand at 300.482 kb on - strand at 300.482 kb on - strand at 300.488 kb on + strand at 300.561 kb on - strand at 300.570 kb on + strand at 300.570 kb on + strand at 300.570 kb on + strand at 300.705 kb on + strand, within ABIE53_004129 at 300.705 kb on + strand, within ABIE53_004129 at 300.706 kb on - strand, within ABIE53_004129 at 300.706 kb on - strand, within ABIE53_004129 at 300.706 kb on - strand, within ABIE53_004129 at 300.706 kb on - strand, within ABIE53_004129 at 300.706 kb on - strand, within ABIE53_004129 at 300.706 kb on - strand, within ABIE53_004129 at 300.706 kb on - strand, within ABIE53_004129 at 300.727 kb on - strand, within ABIE53_004129 at 301.044 kb on - strand, within ABIE53_004129 at 301.044 kb on - strand, within ABIE53_004129 at 301.056 kb on + strand, within ABIE53_004129 at 301.057 kb on - strand, within ABIE53_004129 at 301.555 kb on + strand, within ABIE53_004130 at 302.028 kb on + strand at 302.028 kb on + strand at 302.028 kb on + strand at 302.028 kb on + strand at 302.028 kb on + strand at 302.028 kb on + strand at 302.028 kb on + strand at 302.029 kb on - strand at 302.029 kb on - strand at 302.029 kb on - strand at 302.029 kb on - strand at 302.029 kb on - strand at 302.029 kb on - strand at 302.029 kb on - strand at 302.029 kb on - strand at 302.029 kb on - strand at 302.029 kb on - strand at 302.029 kb on - strand at 302.029 kb on - strand at 302.031 kb on + strand at 302.031 kb on + strand at 302.031 kb on + strand at 302.032 kb on - strand at 302.032 kb on - strand at 302.533 kb on + strand, within ABIE53_004131 at 302.533 kb on + strand, within ABIE53_004131 at 302.746 kb on + strand, within ABIE53_004131 at 302.746 kb on + strand, within ABIE53_004131 at 302.746 kb on + strand, within ABIE53_004131 at 302.747 kb on - strand, within ABIE53_004131 at 302.747 kb on - strand, within ABIE53_004131
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Tricalcium phosphate 0.5 g/L remove 300,276 + ABIE53_004128 0.61 +0.2 300,276 + ABIE53_004128 0.61 -1.3 300,276 + ABIE53_004128 0.61 +0.7 300,276 + ABIE53_004128 0.61 -0.3 300,276 + ABIE53_004128 0.61 -0.5 300,276 + ABIE53_004128 0.61 -1.5 300,276 + ABIE53_004128 0.61 +0.2 300,276 + ABIE53_004128 0.61 -1.9 300,276 + ABIE53_004128 0.61 +3.0 300,276 + ABIE53_004128 0.61 +0.7 300,276 + ABIE53_004128 0.61 +1.1 300,277 - ABIE53_004128 0.61 -1.1 300,277 - ABIE53_004128 0.61 +0.7 300,277 - ABIE53_004128 0.61 +1.0 300,277 - ABIE53_004128 0.61 -0.4 300,277 - ABIE53_004128 0.61 +1.7 300,277 - ABIE53_004128 0.61 -1.4 300,296 + ABIE53_004128 0.64 +0.7 300,296 + ABIE53_004128 0.64 -0.3 300,296 + ABIE53_004128 0.64 -1.2 300,297 - ABIE53_004128 0.64 -1.0 300,297 - ABIE53_004128 0.64 +2.3 300,297 - ABIE53_004128 0.64 +0.5 300,297 - ABIE53_004128 0.64 +0.1 300,481 + -0.2 300,481 + -1.3 300,481 + +2.8 300,482 - -1.6 300,482 - +0.7 300,482 - +2.3 300,482 - -0.8 300,488 + +0.7 300,561 - -0.1 300,570 + +0.7 300,570 + -1.8 300,570 + +1.0 300,705 + ABIE53_004129 0.17 -1.0 300,705 + ABIE53_004129 0.17 +0.7 300,706 - ABIE53_004129 0.17 -1.7 300,706 - ABIE53_004129 0.17 -0.5 300,706 - ABIE53_004129 0.17 +1.7 300,706 - ABIE53_004129 0.17 +0.7 300,706 - ABIE53_004129 0.17 +0.7 300,706 - ABIE53_004129 0.17 +2.5 300,706 - ABIE53_004129 0.17 -0.8 300,727 - ABIE53_004129 0.20 -1.6 301,044 - ABIE53_004129 0.69 -0.3 301,044 - ABIE53_004129 0.69 -3.1 301,056 + ABIE53_004129 0.71 +0.3 301,057 - ABIE53_004129 0.71 +1.5 301,555 + ABIE53_004130 0.45 -2.4 302,028 + -0.2 302,028 + -3.1 302,028 + -1.7 302,028 + +1.7 302,028 + +0.7 302,028 + +3.0 302,028 + -0.3 302,029 - -0.6 302,029 - -0.8 302,029 - +0.0 302,029 - -0.2 302,029 - -1.3 302,029 - +1.1 302,029 - -2.3 302,029 - -1.7 302,029 - +0.7 302,029 - -1.1 302,029 - +1.7 302,029 - -0.0 302,031 + -0.3 302,031 + -0.9 302,031 + -2.1 302,032 - -1.9 302,032 - +0.4 302,533 + ABIE53_004131 0.24 -1.6 302,533 + ABIE53_004131 0.24 -1.3 302,746 + ABIE53_004131 0.32 +0.1 302,746 + ABIE53_004131 0.32 +0.7 302,746 + ABIE53_004131 0.32 -2.9 302,747 - ABIE53_004131 0.33 -1.8 302,747 - ABIE53_004131 0.33 +0.7
Or see this region's nucleotide sequence