Experiment: BHIS with Antimycin A 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4552 and BT4553 are separated by 81 nucleotides BT4553 and BT4554 are separated by 70 nucleotides BT4554 and BT4555 are separated by 162 nucleotides
BT4552: BT4552 - two-component system sensor histidine kinase (NCBI ptt file), at 5,973,512 to 5,975,419
BT4552
BT4553: BT4553 - conserved hypothetical protein (NCBI ptt file), at 5,975,501 to 5,976,160
BT4553
BT4554: BT4554 - purine nucleoside phosphorylase II (NCBI ptt file), at 5,976,231 to 5,977,109
BT4554
BT4555: BT4555 - conserved hypothetical protein (NCBI ptt file), at 5,977,272 to 5,978,273
BT4555
Position (kb)
5976
5977
5978 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5975.287 kb on + strand at 5975.291 kb on - strand at 5975.336 kb on - strand at 5975.360 kb on - strand at 5975.408 kb on + strand at 5975.472 kb on - strand at 5975.477 kb on + strand at 5975.501 kb on - strand at 5975.562 kb on + strand at 5975.563 kb on - strand at 5975.601 kb on + strand, within BT4553 at 5975.602 kb on - strand, within BT4553 at 5975.750 kb on - strand, within BT4553 at 5975.753 kb on + strand, within BT4553 at 5975.754 kb on - strand, within BT4553 at 5975.774 kb on + strand, within BT4553 at 5975.782 kb on + strand, within BT4553 at 5975.782 kb on + strand, within BT4553 at 5975.783 kb on - strand, within BT4553 at 5975.900 kb on - strand, within BT4553 at 5975.973 kb on + strand, within BT4553 at 5975.995 kb on + strand, within BT4553 at 5975.996 kb on - strand, within BT4553 at 5976.009 kb on + strand, within BT4553 at 5976.056 kb on - strand, within BT4553 at 5976.073 kb on + strand, within BT4553 at 5976.073 kb on + strand, within BT4553 at 5976.145 kb on + strand at 5976.186 kb on + strand at 5976.187 kb on - strand at 5976.222 kb on + strand at 5976.223 kb on - strand at 5976.224 kb on + strand at 5976.225 kb on - strand at 5976.233 kb on - strand at 5976.235 kb on - strand at 5976.279 kb on - strand at 5976.279 kb on - strand at 5976.342 kb on - strand, within BT4554 at 5976.414 kb on - strand, within BT4554 at 5976.522 kb on + strand, within BT4554 at 5976.759 kb on - strand, within BT4554 at 5976.769 kb on - strand, within BT4554 at 5976.769 kb on - strand, within BT4554 at 5976.769 kb on - strand, within BT4554 at 5976.771 kb on + strand, within BT4554 at 5976.771 kb on + strand, within BT4554 at 5976.908 kb on + strand, within BT4554 at 5976.908 kb on + strand, within BT4554 at 5976.908 kb on + strand, within BT4554 at 5976.908 kb on + strand, within BT4554 at 5976.908 kb on + strand, within BT4554 at 5976.909 kb on - strand, within BT4554 at 5976.915 kb on - strand, within BT4554 at 5977.020 kb on - strand, within BT4554 at 5977.145 kb on + strand at 5977.147 kb on + strand at 5977.149 kb on + strand at 5977.149 kb on + strand at 5977.274 kb on - strand at 5977.378 kb on - strand, within BT4555 at 5977.470 kb on - strand, within BT4555 at 5977.607 kb on + strand, within BT4555 at 5977.673 kb on + strand, within BT4555 at 5977.674 kb on - strand, within BT4555 at 5977.674 kb on - strand, within BT4555 at 5977.743 kb on + strand, within BT4555 at 5977.887 kb on + strand, within BT4555 at 5977.983 kb on + strand, within BT4555 at 5977.995 kb on + strand, within BT4555 at 5977.995 kb on + strand at 5978.103 kb on - strand, within BT4555 at 5978.103 kb on - strand, within BT4555 at 5978.104 kb on + strand, within BT4555 at 5978.105 kb on - strand, within BT4555
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Antimycin A 0.2 mM remove 5,975,287 + +1.1 5,975,291 - -1.7 5,975,336 - +1.4 5,975,360 - -0.9 5,975,408 + -0.6 5,975,472 - -2.0 5,975,477 + -0.6 5,975,501 - -2.0 5,975,562 + +1.0 5,975,563 - -0.0 5,975,601 + BT4553 0.15 -2.0 5,975,602 - BT4553 0.15 -2.1 5,975,750 - BT4553 0.38 -0.4 5,975,753 + BT4553 0.38 -2.2 5,975,754 - BT4553 0.38 -1.6 5,975,774 + BT4553 0.41 -0.0 5,975,782 + BT4553 0.43 -1.2 5,975,782 + BT4553 0.43 -1.6 5,975,783 - BT4553 0.43 -1.8 5,975,900 - BT4553 0.60 -0.0 5,975,973 + BT4553 0.72 -1.1 5,975,995 + BT4553 0.75 -1.1 5,975,996 - BT4553 0.75 -1.0 5,976,009 + BT4553 0.77 +0.6 5,976,056 - BT4553 0.84 -2.8 5,976,073 + BT4553 0.87 -0.3 5,976,073 + BT4553 0.87 +1.3 5,976,145 + -0.0 5,976,186 + -2.0 5,976,187 - -3.0 5,976,222 + -0.0 5,976,223 - -2.8 5,976,224 + -0.0 5,976,225 - -2.2 5,976,233 - -0.0 5,976,235 - -1.6 5,976,279 - -0.7 5,976,279 - -0.4 5,976,342 - BT4554 0.13 -2.2 5,976,414 - BT4554 0.21 -2.8 5,976,522 + BT4554 0.33 -2.0 5,976,759 - BT4554 0.60 -2.3 5,976,769 - BT4554 0.61 -0.0 5,976,769 - BT4554 0.61 -2.2 5,976,769 - BT4554 0.61 -2.3 5,976,771 + BT4554 0.61 -0.9 5,976,771 + BT4554 0.61 -2.8 5,976,908 + BT4554 0.77 -2.3 5,976,908 + BT4554 0.77 -2.0 5,976,908 + BT4554 0.77 -3.1 5,976,908 + BT4554 0.77 -0.6 5,976,908 + BT4554 0.77 -1.3 5,976,909 - BT4554 0.77 -1.1 5,976,915 - BT4554 0.78 -3.2 5,977,020 - BT4554 0.90 -1.9 5,977,145 + -1.9 5,977,147 + -2.3 5,977,149 + -1.2 5,977,149 + +1.0 5,977,274 - -0.2 5,977,378 - BT4555 0.11 -0.6 5,977,470 - BT4555 0.20 -0.0 5,977,607 + BT4555 0.33 -1.0 5,977,673 + BT4555 0.40 -0.8 5,977,674 - BT4555 0.40 -0.0 5,977,674 - BT4555 0.40 -0.4 5,977,743 + BT4555 0.47 +1.0 5,977,887 + BT4555 0.61 -1.5 5,977,983 + BT4555 0.71 -1.2 5,977,995 + BT4555 0.72 -1.6 5,977,995 + -1.6 5,978,103 - BT4555 0.83 -1.6 5,978,103 - BT4555 0.83 -0.7 5,978,104 + BT4555 0.83 -0.6 5,978,105 - BT4555 0.83 +1.0
Or see this region's nucleotide sequence