Experiment: Nitrate/ lactate media with Nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0384 and Psest_0385 are separated by 313 nucleotides Psest_0385 and Psest_0386 are separated by 0 nucleotides Psest_0386 and Psest_0387 overlap by 11 nucleotides
Psest_0384: Psest_0384 - outer membrane porin, OprD family., at 413,248 to 414,519
_0384
Psest_0385: Psest_0385 - Conserved secreted protein, at 414,833 to 415,336
_0385
Psest_0386: Psest_0386 - Membrane protease subunits, stomatin/prohibitin homologs, at 415,337 to 416,095
_0386
Psest_0387: Psest_0387 - Membrane-bound serine protease (ClpP class), at 416,085 to 417,464
_0387
Position (kb) 414
415
416 Strain fitness (log2 ratio) -2
-1
0
1
2 at 413.870 kb on + strand, within Psest_0384 at 414.190 kb on + strand, within Psest_0384 at 414.212 kb on + strand, within Psest_0384 at 414.212 kb on + strand, within Psest_0384 at 414.212 kb on + strand, within Psest_0384 at 414.213 kb on - strand, within Psest_0384 at 414.290 kb on + strand, within Psest_0384 at 414.290 kb on + strand, within Psest_0384 at 414.380 kb on + strand, within Psest_0384 at 414.380 kb on + strand, within Psest_0384 at 414.381 kb on - strand, within Psest_0384 at 414.381 kb on - strand, within Psest_0384 at 414.381 kb on - strand, within Psest_0384 at 414.381 kb on - strand, within Psest_0384 at 414.381 kb on - strand, within Psest_0384 at 414.520 kb on + strand at 414.690 kb on + strand at 414.691 kb on - strand at 414.719 kb on - strand at 414.719 kb on - strand at 414.771 kb on - strand at 414.847 kb on - strand at 415.128 kb on + strand, within Psest_0385 at 415.129 kb on - strand, within Psest_0385 at 415.129 kb on - strand, within Psest_0385 at 415.328 kb on + strand at 415.328 kb on + strand at 415.328 kb on + strand at 415.328 kb on + strand at 415.328 kb on + strand at 415.328 kb on + strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.443 kb on + strand, within Psest_0386 at 415.443 kb on + strand, within Psest_0386 at 415.444 kb on - strand, within Psest_0386 at 415.782 kb on + strand, within Psest_0386 at 415.830 kb on + strand, within Psest_0386 at 415.830 kb on + strand, within Psest_0386 at 415.830 kb on + strand, within Psest_0386 at 415.830 kb on + strand, within Psest_0386 at 415.830 kb on + strand, within Psest_0386 at 415.830 kb on + strand, within Psest_0386 at 415.830 kb on + strand, within Psest_0386 at 415.830 kb on + strand, within Psest_0386 at 415.830 kb on + strand, within Psest_0386 at 415.830 kb on + strand, within Psest_0386 at 415.831 kb on - strand, within Psest_0386 at 415.831 kb on - strand, within Psest_0386 at 415.831 kb on - strand, within Psest_0386 at 415.831 kb on - strand, within Psest_0386 at 415.831 kb on - strand, within Psest_0386 at 415.831 kb on - strand, within Psest_0386 at 415.831 kb on - strand, within Psest_0386 at 416.060 kb on + strand at 416.060 kb on + strand at 416.089 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate/ lactate media with Nitrate 20mM remove 413,870 + Psest_0384 0.49 -0.5 414,190 + Psest_0384 0.74 +1.0 414,212 + Psest_0384 0.76 -2.1 414,212 + Psest_0384 0.76 -0.6 414,212 + Psest_0384 0.76 +0.4 414,213 - Psest_0384 0.76 -0.6 414,290 + Psest_0384 0.82 +1.0 414,290 + Psest_0384 0.82 +0.7 414,380 + Psest_0384 0.89 +0.0 414,380 + Psest_0384 0.89 -2.1 414,381 - Psest_0384 0.89 +0.0 414,381 - Psest_0384 0.89 -0.3 414,381 - Psest_0384 0.89 -0.6 414,381 - Psest_0384 0.89 +2.0 414,381 - Psest_0384 0.89 +0.1 414,520 + -0.6 414,690 + -0.2 414,691 - -0.1 414,719 - +1.6 414,719 - +0.8 414,771 - -1.2 414,847 - +0.4 415,128 + Psest_0385 0.59 -1.0 415,129 - Psest_0385 0.59 -1.0 415,129 - Psest_0385 0.59 -1.7 415,328 + -0.3 415,328 + -0.6 415,328 + +1.0 415,328 + -0.1 415,328 + -1.0 415,328 + +0.7 415,329 - +0.2 415,329 - +1.4 415,329 - -0.8 415,329 - -0.8 415,329 - -0.1 415,329 - +0.2 415,329 - -0.5 415,329 - -1.2 415,329 - +0.8 415,329 - +0.0 415,329 - -0.6 415,329 - -1.1 415,443 + Psest_0386 0.14 +0.7 415,443 + Psest_0386 0.14 +0.9 415,444 - Psest_0386 0.14 +0.4 415,782 + Psest_0386 0.59 -1.9 415,830 + Psest_0386 0.65 -0.5 415,830 + Psest_0386 0.65 -0.4 415,830 + Psest_0386 0.65 -0.0 415,830 + Psest_0386 0.65 +0.0 415,830 + Psest_0386 0.65 -0.8 415,830 + Psest_0386 0.65 +0.2 415,830 + Psest_0386 0.65 -0.6 415,830 + Psest_0386 0.65 +0.4 415,830 + Psest_0386 0.65 -0.1 415,830 + Psest_0386 0.65 -0.6 415,831 - Psest_0386 0.65 +0.2 415,831 - Psest_0386 0.65 +0.3 415,831 - Psest_0386 0.65 -0.5 415,831 - Psest_0386 0.65 -1.1 415,831 - Psest_0386 0.65 -0.5 415,831 - Psest_0386 0.65 -1.1 415,831 - Psest_0386 0.65 -0.4 416,060 + +0.7 416,060 + +0.7 416,089 - +0.8
Or see this region's nucleotide sequence