Experiment: Nitrate/ lactate media with Nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3333 and Psest_3334 are separated by 40 nucleotides Psest_3334 and Psest_3336 are separated by 353 nucleotides
Psest_3333: Psest_3333 - K+-dependent Na+/Ca+ exchanger related-protein, at 3,498,647 to 3,499,735
_3333
Psest_3334: Psest_3334 - Integral membrane protein CcmA involved in cell shape determination, at 3,499,776 to 3,500,198
_3334
Psest_3336: Psest_3336 - Membrane proteins related to metalloendopeptidases, at 3,500,552 to 3,501,535
_3336
Position (kb)
3499
3500
3501 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3498.862 kb on + strand, within Psest_3333 at 3498.892 kb on - strand, within Psest_3333 at 3498.892 kb on - strand, within Psest_3333 at 3498.892 kb on - strand, within Psest_3333 at 3498.892 kb on - strand, within Psest_3333 at 3498.892 kb on - strand, within Psest_3333 at 3498.892 kb on - strand, within Psest_3333 at 3498.892 kb on - strand, within Psest_3333 at 3499.064 kb on + strand, within Psest_3333 at 3499.083 kb on + strand, within Psest_3333 at 3499.083 kb on + strand, within Psest_3333 at 3499.083 kb on + strand, within Psest_3333 at 3499.084 kb on - strand, within Psest_3333 at 3499.084 kb on - strand, within Psest_3333 at 3499.084 kb on - strand, within Psest_3333 at 3499.084 kb on - strand, within Psest_3333 at 3499.580 kb on + strand, within Psest_3333 at 3499.580 kb on + strand, within Psest_3333 at 3499.580 kb on + strand, within Psest_3333 at 3499.583 kb on + strand, within Psest_3333 at 3499.583 kb on + strand, within Psest_3333 at 3499.583 kb on + strand, within Psest_3333 at 3499.584 kb on - strand, within Psest_3333 at 3499.584 kb on - strand, within Psest_3333 at 3499.584 kb on - strand, within Psest_3333 at 3499.592 kb on + strand, within Psest_3333 at 3499.592 kb on + strand, within Psest_3333 at 3499.593 kb on - strand, within Psest_3333 at 3499.593 kb on - strand, within Psest_3333 at 3499.601 kb on + strand, within Psest_3333 at 3499.601 kb on + strand, within Psest_3333 at 3499.664 kb on + strand at 3499.774 kb on - strand at 3499.990 kb on + strand, within Psest_3334 at 3499.990 kb on + strand, within Psest_3334 at 3499.991 kb on - strand, within Psest_3334 at 3499.991 kb on - strand, within Psest_3334 at 3499.991 kb on - strand, within Psest_3334 at 3499.991 kb on - strand, within Psest_3334 at 3499.995 kb on - strand, within Psest_3334 at 3499.995 kb on - strand, within Psest_3334 at 3500.028 kb on + strand, within Psest_3334 at 3500.029 kb on - strand, within Psest_3334 at 3500.203 kb on + strand at 3500.203 kb on + strand at 3500.204 kb on - strand at 3500.204 kb on - strand at 3500.204 kb on - strand at 3500.204 kb on - strand at 3500.435 kb on + strand at 3500.435 kb on + strand at 3500.436 kb on - strand at 3500.436 kb on - strand at 3500.554 kb on - strand at 3500.554 kb on - strand at 3500.554 kb on - strand at 3500.684 kb on + strand, within Psest_3336 at 3500.729 kb on + strand, within Psest_3336 at 3500.729 kb on + strand, within Psest_3336 at 3500.729 kb on + strand, within Psest_3336 at 3500.729 kb on + strand, within Psest_3336 at 3500.729 kb on + strand, within Psest_3336 at 3501.035 kb on + strand, within Psest_3336 at 3501.036 kb on - strand, within Psest_3336
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate/ lactate media with Nitrate 20mM remove 3,498,862 + Psest_3333 0.20 +0.4 3,498,892 - Psest_3333 0.22 +0.0 3,498,892 - Psest_3333 0.22 +0.5 3,498,892 - Psest_3333 0.22 -0.6 3,498,892 - Psest_3333 0.22 +0.4 3,498,892 - Psest_3333 0.22 -0.4 3,498,892 - Psest_3333 0.22 +0.4 3,498,892 - Psest_3333 0.22 -0.2 3,499,064 + Psest_3333 0.38 -0.1 3,499,083 + Psest_3333 0.40 -1.1 3,499,083 + Psest_3333 0.40 -2.0 3,499,083 + Psest_3333 0.40 -0.4 3,499,084 - Psest_3333 0.40 +0.5 3,499,084 - Psest_3333 0.40 -1.3 3,499,084 - Psest_3333 0.40 -0.5 3,499,084 - Psest_3333 0.40 +0.9 3,499,580 + Psest_3333 0.86 -0.9 3,499,580 + Psest_3333 0.86 +1.9 3,499,580 + Psest_3333 0.86 +1.2 3,499,583 + Psest_3333 0.86 -1.4 3,499,583 + Psest_3333 0.86 +0.8 3,499,583 + Psest_3333 0.86 +0.2 3,499,584 - Psest_3333 0.86 +0.4 3,499,584 - Psest_3333 0.86 +0.1 3,499,584 - Psest_3333 0.86 -0.3 3,499,592 + Psest_3333 0.87 -0.6 3,499,592 + Psest_3333 0.87 +0.5 3,499,593 - Psest_3333 0.87 -0.3 3,499,593 - Psest_3333 0.87 +0.7 3,499,601 + Psest_3333 0.88 +0.5 3,499,601 + Psest_3333 0.88 -0.2 3,499,664 + -0.1 3,499,774 - +1.2 3,499,990 + Psest_3334 0.51 -1.7 3,499,990 + Psest_3334 0.51 -1.2 3,499,991 - Psest_3334 0.51 -0.2 3,499,991 - Psest_3334 0.51 +1.1 3,499,991 - Psest_3334 0.51 -1.5 3,499,991 - Psest_3334 0.51 -0.9 3,499,995 - Psest_3334 0.52 -0.6 3,499,995 - Psest_3334 0.52 -0.2 3,500,028 + Psest_3334 0.60 -1.1 3,500,029 - Psest_3334 0.60 -0.4 3,500,203 + -0.2 3,500,203 + +1.9 3,500,204 - -0.1 3,500,204 - -1.1 3,500,204 - -0.4 3,500,204 - +0.6 3,500,435 + -0.8 3,500,435 + -0.4 3,500,436 - -0.3 3,500,436 - -1.2 3,500,554 - -0.8 3,500,554 - -0.6 3,500,554 - +0.4 3,500,684 + Psest_3336 0.13 +0.9 3,500,729 + Psest_3336 0.18 +0.8 3,500,729 + Psest_3336 0.18 -0.1 3,500,729 + Psest_3336 0.18 -0.1 3,500,729 + Psest_3336 0.18 -0.6 3,500,729 + Psest_3336 0.18 +1.8 3,501,035 + Psest_3336 0.49 -0.4 3,501,036 - Psest_3336 0.49 +0.4
Or see this region's nucleotide sequence