Experiment: Nitrate/ lactate media with Nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2343 and Psest_2344 are separated by 106 nucleotides Psest_2344 and Psest_2346 are separated by 346 nucleotides
Psest_2343: Psest_2343 - Mg-dependent DNase, at 2,497,342 to 2,498,148
_2343
Psest_2344: Psest_2344 - Methyl-accepting chemotaxis protein, at 2,498,255 to 2,499,724
_2344
Psest_2346: Psest_2346 - ABC-type nitrate/sulfonate/bicarbonate transport system, permease component, at 2,500,071 to 2,500,961
_2346
Position (kb)
2498
2499
2500 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2497.843 kb on + strand, within Psest_2343 at 2497.844 kb on - strand, within Psest_2343 at 2497.844 kb on - strand, within Psest_2343 at 2497.850 kb on - strand, within Psest_2343 at 2497.976 kb on - strand, within Psest_2343 at 2498.032 kb on + strand, within Psest_2343 at 2498.033 kb on - strand, within Psest_2343 at 2498.237 kb on + strand at 2498.239 kb on + strand at 2498.239 kb on + strand at 2498.239 kb on + strand at 2498.239 kb on + strand at 2498.240 kb on - strand at 2498.240 kb on - strand at 2498.240 kb on - strand at 2498.240 kb on - strand at 2498.240 kb on - strand at 2498.288 kb on + strand at 2498.345 kb on + strand at 2498.739 kb on + strand, within Psest_2344 at 2498.752 kb on + strand, within Psest_2344 at 2498.753 kb on - strand, within Psest_2344 at 2498.753 kb on - strand, within Psest_2344 at 2498.968 kb on + strand, within Psest_2344 at 2499.236 kb on - strand, within Psest_2344 at 2499.629 kb on - strand at 2499.923 kb on + strand at 2499.931 kb on + strand at 2500.198 kb on + strand, within Psest_2346 at 2500.227 kb on + strand, within Psest_2346 at 2500.350 kb on + strand, within Psest_2346 at 2500.426 kb on + strand, within Psest_2346 at 2500.426 kb on + strand, within Psest_2346 at 2500.427 kb on - strand, within Psest_2346 at 2500.427 kb on - strand, within Psest_2346
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate/ lactate media with Nitrate 20mM remove 2,497,843 + Psest_2343 0.62 -0.2 2,497,844 - Psest_2343 0.62 -0.8 2,497,844 - Psest_2343 0.62 -0.2 2,497,850 - Psest_2343 0.63 +0.3 2,497,976 - Psest_2343 0.79 -0.4 2,498,032 + Psest_2343 0.86 +0.8 2,498,033 - Psest_2343 0.86 -0.9 2,498,237 + -0.6 2,498,239 + -1.2 2,498,239 + +0.1 2,498,239 + +0.7 2,498,239 + +2.1 2,498,240 - -0.5 2,498,240 - +0.3 2,498,240 - -0.5 2,498,240 - -2.0 2,498,240 - +0.2 2,498,288 + -0.2 2,498,345 + -1.1 2,498,739 + Psest_2344 0.33 -0.2 2,498,752 + Psest_2344 0.34 -1.4 2,498,753 - Psest_2344 0.34 -1.5 2,498,753 - Psest_2344 0.34 +1.1 2,498,968 + Psest_2344 0.49 -0.1 2,499,236 - Psest_2344 0.67 -0.0 2,499,629 - -0.2 2,499,923 + +1.8 2,499,931 + -0.4 2,500,198 + Psest_2346 0.14 -0.2 2,500,227 + Psest_2346 0.18 +0.7 2,500,350 + Psest_2346 0.31 -0.0 2,500,426 + Psest_2346 0.40 -0.3 2,500,426 + Psest_2346 0.40 -0.1 2,500,427 - Psest_2346 0.40 +0.1 2,500,427 - Psest_2346 0.40 -0.4
Or see this region's nucleotide sequence