Experiment: Nitrate/ lactate media with Nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1958 and Psest_1959 are separated by 75 nucleotides Psest_1959 and Psest_1960 are separated by 150 nucleotides Psest_1960 and Psest_1961 are separated by 46 nucleotides Psest_1961 and Psest_1962 are separated by 211 nucleotides
Psest_1958: Psest_1958 - Glutaredoxin-like domain (DUF836)., at 2,075,288 to 2,075,536
_1958
Psest_1959: Psest_1959 - tRNA_Pro_CGG, at 2,075,612 to 2,075,688
_1959
Psest_1960: Psest_1960 - Bacterial protein of unknown function (DUF903)., at 2,075,839 to 2,076,060
_1960
Psest_1961: Psest_1961 - molybdopterin-guanine dinucleotide biosynthesis protein A, proteobacterial, at 2,076,107 to 2,076,715
_1961
Psest_1962: Psest_1962 - Fucose 4-O-acetylase and related acetyltransferases, at 2,076,927 to 2,077,955
_1962
Position (kb)
2076
2077 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2075.162 kb on + strand at 2075.162 kb on + strand at 2075.163 kb on - strand at 2075.163 kb on - strand at 2075.163 kb on - strand at 2075.163 kb on - strand at 2075.254 kb on + strand at 2075.254 kb on + strand at 2075.254 kb on + strand at 2075.254 kb on + strand at 2075.255 kb on - strand at 2075.274 kb on - strand at 2075.274 kb on - strand at 2075.391 kb on + strand, within Psest_1958 at 2075.391 kb on + strand, within Psest_1958 at 2075.392 kb on - strand, within Psest_1958 at 2075.392 kb on - strand, within Psest_1958 at 2075.398 kb on + strand, within Psest_1958 at 2075.449 kb on - strand, within Psest_1958 at 2075.861 kb on - strand at 2075.861 kb on - strand at 2075.906 kb on + strand, within Psest_1960 at 2075.906 kb on + strand, within Psest_1960 at 2075.907 kb on - strand, within Psest_1960 at 2075.907 kb on - strand, within Psest_1960 at 2075.934 kb on - strand, within Psest_1960 at 2076.105 kb on + strand at 2076.105 kb on + strand at 2076.105 kb on + strand at 2076.106 kb on - strand at 2076.108 kb on + strand at 2076.108 kb on + strand at 2076.108 kb on + strand at 2076.109 kb on - strand at 2076.109 kb on - strand at 2076.109 kb on - strand at 2076.109 kb on - strand at 2076.109 kb on - strand at 2076.109 kb on - strand at 2076.194 kb on + strand, within Psest_1961 at 2076.194 kb on + strand, within Psest_1961 at 2076.195 kb on - strand, within Psest_1961 at 2076.195 kb on - strand, within Psest_1961 at 2076.195 kb on - strand, within Psest_1961 at 2076.293 kb on - strand, within Psest_1961 at 2076.370 kb on - strand, within Psest_1961 at 2076.370 kb on - strand, within Psest_1961 at 2076.398 kb on + strand, within Psest_1961 at 2076.398 kb on + strand, within Psest_1961 at 2076.398 kb on + strand, within Psest_1961 at 2076.399 kb on - strand, within Psest_1961 at 2076.519 kb on + strand, within Psest_1961 at 2076.519 kb on + strand, within Psest_1961 at 2076.519 kb on + strand, within Psest_1961 at 2076.519 kb on + strand, within Psest_1961 at 2076.519 kb on + strand, within Psest_1961 at 2076.519 kb on + strand, within Psest_1961 at 2076.519 kb on + strand, within Psest_1961 at 2076.520 kb on - strand, within Psest_1961 at 2076.520 kb on - strand, within Psest_1961 at 2076.520 kb on - strand, within Psest_1961 at 2076.520 kb on - strand, within Psest_1961 at 2076.520 kb on - strand, within Psest_1961 at 2076.520 kb on - strand, within Psest_1961 at 2076.560 kb on + strand, within Psest_1961 at 2076.560 kb on + strand, within Psest_1961 at 2076.561 kb on - strand, within Psest_1961 at 2076.561 kb on - strand, within Psest_1961 at 2076.740 kb on + strand at 2076.743 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate/ lactate media with Nitrate 20mM remove 2,075,162 + -0.1 2,075,162 + -0.6 2,075,163 - -0.2 2,075,163 - -0.7 2,075,163 - +0.1 2,075,163 - -0.8 2,075,254 + +0.3 2,075,254 + -2.0 2,075,254 + -0.5 2,075,254 + -0.6 2,075,255 - -0.1 2,075,274 - -1.1 2,075,274 - -0.3 2,075,391 + Psest_1958 0.41 -0.4 2,075,391 + Psest_1958 0.41 +0.7 2,075,392 - Psest_1958 0.42 -0.1 2,075,392 - Psest_1958 0.42 -1.6 2,075,398 + Psest_1958 0.44 +0.0 2,075,449 - Psest_1958 0.65 +0.2 2,075,861 - +0.2 2,075,861 - -0.8 2,075,906 + Psest_1960 0.30 +0.2 2,075,906 + Psest_1960 0.30 +0.6 2,075,907 - Psest_1960 0.31 -0.1 2,075,907 - Psest_1960 0.31 -0.9 2,075,934 - Psest_1960 0.43 -1.3 2,076,105 + -1.9 2,076,105 + +1.0 2,076,105 + +0.1 2,076,106 - -1.7 2,076,108 + +0.6 2,076,108 + +0.4 2,076,108 + +0.7 2,076,109 - -0.7 2,076,109 - +0.2 2,076,109 - -0.6 2,076,109 - +0.3 2,076,109 - +0.9 2,076,109 - +1.2 2,076,194 + Psest_1961 0.14 -1.2 2,076,194 + Psest_1961 0.14 -0.6 2,076,195 - Psest_1961 0.14 -1.4 2,076,195 - Psest_1961 0.14 -1.7 2,076,195 - Psest_1961 0.14 -0.8 2,076,293 - Psest_1961 0.31 -1.8 2,076,370 - Psest_1961 0.43 -1.2 2,076,370 - Psest_1961 0.43 -2.0 2,076,398 + Psest_1961 0.48 -1.9 2,076,398 + Psest_1961 0.48 -0.6 2,076,398 + Psest_1961 0.48 -1.9 2,076,399 - Psest_1961 0.48 -1.6 2,076,519 + Psest_1961 0.68 -0.6 2,076,519 + Psest_1961 0.68 -1.3 2,076,519 + Psest_1961 0.68 +1.2 2,076,519 + Psest_1961 0.68 -2.3 2,076,519 + Psest_1961 0.68 -1.8 2,076,519 + Psest_1961 0.68 -0.7 2,076,519 + Psest_1961 0.68 -1.4 2,076,520 - Psest_1961 0.68 -0.9 2,076,520 - Psest_1961 0.68 -1.6 2,076,520 - Psest_1961 0.68 -1.9 2,076,520 - Psest_1961 0.68 -2.1 2,076,520 - Psest_1961 0.68 -0.8 2,076,520 - Psest_1961 0.68 -2.5 2,076,560 + Psest_1961 0.74 +0.8 2,076,560 + Psest_1961 0.74 -2.4 2,076,561 - Psest_1961 0.75 -1.1 2,076,561 - Psest_1961 0.75 +1.8 2,076,740 + +0.1 2,076,743 - -1.1
Or see this region's nucleotide sequence