Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0106

Experiment: Nitrate/ lactate media with Nitrate 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0104 and Psest_0105 overlap by 8 nucleotidesPsest_0105 and Psest_0106 overlap by 4 nucleotidesPsest_0106 and Psest_0107 are separated by 72 nucleotidesPsest_0107 and Psest_0108 overlap by 4 nucleotides Psest_0104: Psest_0104 - malonate decarboxylase acyl carrier protein, at 103,898 to 104,197 _0104 Psest_0105: Psest_0105 - malonate decarboxylase, beta subunit, at 104,190 to 105,056 _0105 Psest_0106: Psest_0106 - malonate decarboxylase, gamma subunit, at 105,053 to 105,838 _0106 Psest_0107: Psest_0107 - malonate decarboxylase holo-[acyl-carrier-protein] synthase, at 105,911 to 106,528 _0107 Psest_0108: Psest_0108 - malonate decarboxylase, epsilon subunit, at 106,525 to 107,451 _0108 Position (kb) 105 106Strain fitness (log2 ratio) -2 -1 0 1 2at 104.640 kb on + strand, within Psest_0105at 104.640 kb on + strand, within Psest_0105at 104.718 kb on + strand, within Psest_0105at 104.719 kb on - strand, within Psest_0105at 104.719 kb on - strand, within Psest_0105at 104.797 kb on - strand, within Psest_0105at 104.874 kb on + strand, within Psest_0105at 104.874 kb on + strand, within Psest_0105at 104.874 kb on + strand, within Psest_0105at 104.874 kb on + strand, within Psest_0105at 104.874 kb on + strand, within Psest_0105at 104.874 kb on + strand, within Psest_0105at 104.874 kb on + strand, within Psest_0105at 104.874 kb on + strand, within Psest_0105at 104.875 kb on - strand, within Psest_0105at 104.875 kb on - strand, within Psest_0105at 104.875 kb on - strand, within Psest_0105at 105.155 kb on - strand, within Psest_0106at 105.164 kb on + strand, within Psest_0106at 105.164 kb on + strand, within Psest_0106at 105.164 kb on + strand, within Psest_0106at 105.165 kb on - strand, within Psest_0106at 105.165 kb on - strand, within Psest_0106at 105.325 kb on - strand, within Psest_0106at 105.339 kb on - strand, within Psest_0106at 105.621 kb on - strand, within Psest_0106at 105.635 kb on + strand, within Psest_0106at 105.635 kb on + strand, within Psest_0106at 105.636 kb on - strand, within Psest_0106at 105.636 kb on - strand, within Psest_0106at 105.636 kb on - strand, within Psest_0106at 105.636 kb on - strand, within Psest_0106at 105.636 kb on - strand, within Psest_0106at 105.908 kb on + strandat 105.908 kb on + strandat 105.908 kb on + strandat 105.909 kb on - strandat 105.909 kb on - strandat 105.909 kb on - strandat 105.909 kb on - strandat 106.230 kb on - strand, within Psest_0107at 106.765 kb on + strand, within Psest_0108at 106.765 kb on + strand, within Psest_0108at 106.765 kb on + strand, within Psest_0108at 106.765 kb on + strand, within Psest_0108at 106.765 kb on + strand, within Psest_0108at 106.765 kb on + strand, within Psest_0108at 106.765 kb on + strand, within Psest_0108at 106.765 kb on + strand, within Psest_0108at 106.766 kb on - strand, within Psest_0108at 106.766 kb on - strand, within Psest_0108at 106.766 kb on - strand, within Psest_0108at 106.766 kb on - strand, within Psest_0108at 106.766 kb on - strand, within Psest_0108at 106.792 kb on + strand, within Psest_0108at 106.792 kb on + strand, within Psest_0108at 106.792 kb on + strand, within Psest_0108at 106.826 kb on - strand, within Psest_0108at 106.826 kb on - strand, within Psest_0108at 106.826 kb on - strand, within Psest_0108

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate/ lactate media with Nitrate 20mM
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104,640 + Psest_0105 0.52 -1.1
104,640 + Psest_0105 0.52 -0.1
104,718 + Psest_0105 0.61 -0.9
104,719 - Psest_0105 0.61 -1.3
104,719 - Psest_0105 0.61 -0.8
104,797 - Psest_0105 0.70 +0.1
104,874 + Psest_0105 0.79 -0.8
104,874 + Psest_0105 0.79 -0.5
104,874 + Psest_0105 0.79 +0.4
104,874 + Psest_0105 0.79 -0.6
104,874 + Psest_0105 0.79 -0.6
104,874 + Psest_0105 0.79 -0.2
104,874 + Psest_0105 0.79 +0.3
104,874 + Psest_0105 0.79 -0.7
104,875 - Psest_0105 0.79 +1.1
104,875 - Psest_0105 0.79 +0.3
104,875 - Psest_0105 0.79 -2.3
105,155 - Psest_0106 0.13 -1.1
105,164 + Psest_0106 0.14 -0.8
105,164 + Psest_0106 0.14 -1.5
105,164 + Psest_0106 0.14 -0.6
105,165 - Psest_0106 0.14 -1.2
105,165 - Psest_0106 0.14 -0.9
105,325 - Psest_0106 0.35 -1.2
105,339 - Psest_0106 0.36 +0.7
105,621 - Psest_0106 0.72 -1.7
105,635 + Psest_0106 0.74 -0.7
105,635 + Psest_0106 0.74 -1.3
105,636 - Psest_0106 0.74 -1.6
105,636 - Psest_0106 0.74 -0.5
105,636 - Psest_0106 0.74 +0.4
105,636 - Psest_0106 0.74 -0.7
105,636 - Psest_0106 0.74 +1.1
105,908 + +0.3
105,908 + -0.6
105,908 + -0.3
105,909 - +0.5
105,909 - +1.1
105,909 - +0.5
105,909 - -1.2
106,230 - Psest_0107 0.52 +2.1
106,765 + Psest_0108 0.26 +0.5
106,765 + Psest_0108 0.26 +0.3
106,765 + Psest_0108 0.26 -1.0
106,765 + Psest_0108 0.26 +0.8
106,765 + Psest_0108 0.26 -0.6
106,765 + Psest_0108 0.26 +0.4
106,765 + Psest_0108 0.26 +2.4
106,765 + Psest_0108 0.26 +0.2
106,766 - Psest_0108 0.26 -0.1
106,766 - Psest_0108 0.26 -1.8
106,766 - Psest_0108 0.26 -1.3
106,766 - Psest_0108 0.26 -0.3
106,766 - Psest_0108 0.26 -0.9
106,792 + Psest_0108 0.29 +0.5
106,792 + Psest_0108 0.29 +0.3
106,792 + Psest_0108 0.29 -0.4
106,826 - Psest_0108 0.32 +0.8
106,826 - Psest_0108 0.32 -0.3
106,826 - Psest_0108 0.32 -0.6

Or see this region's nucleotide sequence