Experiment: Nitrate/ lactate media with Nitrate 20mM and .05mM Tungsten
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2363 and Psest_2364 overlap by 4 nucleotides Psest_2364 and Psest_2365 are separated by 2 nucleotides Psest_2365 and Psest_2366 are separated by 3 nucleotides
Psest_2363: Psest_2363 - hypothetical protein, at 2,517,117 to 2,517,926
_2363
Psest_2364: Psest_2364 - ABC-type nitrate/sulfonate/bicarbonate transport system, permease component, at 2,517,923 to 2,518,801
_2364
Psest_2365: Psest_2365 - ABC-type nitrate/sulfonate/bicarbonate transport system, ATPase component, at 2,518,804 to 2,519,643
_2365
Psest_2366: Psest_2366 - Homoserine acetyltransferase, at 2,519,647 to 2,520,669
_2366
Position (kb)
2518
2519
2520 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2517.905 kb on + strand at 2518.289 kb on + strand, within Psest_2364 at 2518.289 kb on + strand, within Psest_2364 at 2518.290 kb on - strand, within Psest_2364 at 2518.290 kb on - strand, within Psest_2364 at 2518.349 kb on + strand, within Psest_2364 at 2518.350 kb on - strand, within Psest_2364 at 2518.350 kb on - strand, within Psest_2364 at 2518.350 kb on - strand, within Psest_2364 at 2518.616 kb on + strand, within Psest_2364 at 2518.617 kb on - strand, within Psest_2364 at 2518.685 kb on + strand, within Psest_2364 at 2518.685 kb on + strand, within Psest_2364 at 2518.685 kb on + strand, within Psest_2364 at 2518.685 kb on + strand, within Psest_2364 at 2518.685 kb on + strand, within Psest_2364 at 2518.685 kb on + strand, within Psest_2364 at 2518.686 kb on - strand, within Psest_2364 at 2518.686 kb on - strand, within Psest_2364 at 2518.686 kb on - strand, within Psest_2364 at 2519.431 kb on + strand, within Psest_2365 at 2519.432 kb on - strand, within Psest_2365 at 2519.432 kb on - strand, within Psest_2365 at 2519.432 kb on - strand, within Psest_2365 at 2519.432 kb on - strand, within Psest_2365 at 2519.432 kb on - strand, within Psest_2365 at 2519.660 kb on - strand at 2519.807 kb on + strand, within Psest_2366 at 2519.807 kb on + strand, within Psest_2366 at 2519.808 kb on - strand, within Psest_2366 at 2519.808 kb on - strand, within Psest_2366 at 2519.808 kb on - strand, within Psest_2366 at 2519.916 kb on + strand, within Psest_2366 at 2519.917 kb on - strand, within Psest_2366 at 2519.917 kb on - strand, within Psest_2366 at 2520.029 kb on + strand, within Psest_2366 at 2520.030 kb on - strand, within Psest_2366 at 2520.044 kb on + strand, within Psest_2366 at 2520.045 kb on - strand, within Psest_2366 at 2520.045 kb on - strand, within Psest_2366 at 2520.105 kb on - strand, within Psest_2366 at 2520.308 kb on + strand, within Psest_2366 at 2520.308 kb on + strand, within Psest_2366 at 2520.309 kb on - strand, within Psest_2366 at 2520.309 kb on - strand, within Psest_2366 at 2520.340 kb on + strand, within Psest_2366 at 2520.340 kb on + strand, within Psest_2366 at 2520.341 kb on - strand, within Psest_2366 at 2520.341 kb on - strand, within Psest_2366 at 2520.341 kb on - strand, within Psest_2366 at 2520.341 kb on - strand, within Psest_2366 at 2520.341 kb on - strand, within Psest_2366 at 2520.341 kb on - strand, within Psest_2366 at 2520.341 kb on - strand, within Psest_2366 at 2520.341 kb on - strand, within Psest_2366 at 2520.521 kb on + strand, within Psest_2366 at 2520.522 kb on - strand, within Psest_2366 at 2520.522 kb on - strand, within Psest_2366
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate/ lactate media with Nitrate 20mM and .05mM Tungsten remove 2,517,905 + -0.8 2,518,289 + Psest_2364 0.42 +1.5 2,518,289 + Psest_2364 0.42 +0.3 2,518,290 - Psest_2364 0.42 +0.3 2,518,290 - Psest_2364 0.42 +2.9 2,518,349 + Psest_2364 0.48 -0.6 2,518,350 - Psest_2364 0.49 -0.2 2,518,350 - Psest_2364 0.49 -0.4 2,518,350 - Psest_2364 0.49 +1.0 2,518,616 + Psest_2364 0.79 +1.3 2,518,617 - Psest_2364 0.79 -0.1 2,518,685 + Psest_2364 0.87 -0.9 2,518,685 + Psest_2364 0.87 -0.4 2,518,685 + Psest_2364 0.87 -0.0 2,518,685 + Psest_2364 0.87 +0.3 2,518,685 + Psest_2364 0.87 -1.1 2,518,685 + Psest_2364 0.87 +0.3 2,518,686 - Psest_2364 0.87 -0.7 2,518,686 - Psest_2364 0.87 +0.1 2,518,686 - Psest_2364 0.87 -0.0 2,519,431 + Psest_2365 0.75 -0.4 2,519,432 - Psest_2365 0.75 +0.6 2,519,432 - Psest_2365 0.75 +0.8 2,519,432 - Psest_2365 0.75 +0.5 2,519,432 - Psest_2365 0.75 +1.4 2,519,432 - Psest_2365 0.75 +0.1 2,519,660 - -1.0 2,519,807 + Psest_2366 0.16 -0.3 2,519,807 + Psest_2366 0.16 +1.1 2,519,808 - Psest_2366 0.16 +0.6 2,519,808 - Psest_2366 0.16 +1.6 2,519,808 - Psest_2366 0.16 -0.7 2,519,916 + Psest_2366 0.26 +0.4 2,519,917 - Psest_2366 0.26 +0.2 2,519,917 - Psest_2366 0.26 -0.5 2,520,029 + Psest_2366 0.37 +0.7 2,520,030 - Psest_2366 0.37 -0.3 2,520,044 + Psest_2366 0.39 +0.1 2,520,045 - Psest_2366 0.39 -1.7 2,520,045 - Psest_2366 0.39 -0.3 2,520,105 - Psest_2366 0.45 -0.3 2,520,308 + Psest_2366 0.65 -0.2 2,520,308 + Psest_2366 0.65 -0.8 2,520,309 - Psest_2366 0.65 -2.6 2,520,309 - Psest_2366 0.65 +1.6 2,520,340 + Psest_2366 0.68 -1.4 2,520,340 + Psest_2366 0.68 -0.3 2,520,341 - Psest_2366 0.68 +0.5 2,520,341 - Psest_2366 0.68 +0.5 2,520,341 - Psest_2366 0.68 -0.1 2,520,341 - Psest_2366 0.68 -0.0 2,520,341 - Psest_2366 0.68 +0.7 2,520,341 - Psest_2366 0.68 -0.3 2,520,341 - Psest_2366 0.68 +0.3 2,520,341 - Psest_2366 0.68 -1.7 2,520,521 + Psest_2366 0.85 -0.0 2,520,522 - Psest_2366 0.86 +0.2 2,520,522 - Psest_2366 0.86 -1.1
Or see this region's nucleotide sequence