Experiment: Nitrate/ lactate media with Nitrate 20mM and .05mM Tungsten
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2565 and Psest_2566 are separated by 136 nucleotides Psest_2566 and Psest_2567 are separated by 358 nucleotides
Psest_2565: Psest_2565 - Transcriptional regulator, at 2,747,551 to 2,748,459
_2565
Psest_2566: Psest_2566 - Uncharacterized protein conserved in bacteria, at 2,748,596 to 2,749,075
_2566
Psest_2567: Psest_2567 - PEP phosphonomutase and related enzymes, at 2,749,434 to 2,750,270
_2567
Position (kb)
2748
2749
2750 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2747.637 kb on + strand at 2747.637 kb on + strand at 2747.637 kb on + strand at 2747.637 kb on + strand at 2747.637 kb on + strand at 2747.637 kb on + strand at 2747.638 kb on - strand at 2747.638 kb on - strand at 2747.638 kb on - strand at 2747.638 kb on - strand at 2747.638 kb on - strand at 2747.638 kb on - strand at 2747.638 kb on - strand at 2747.638 kb on - strand at 2748.071 kb on + strand, within Psest_2565 at 2748.166 kb on - strand, within Psest_2565 at 2748.166 kb on - strand, within Psest_2565 at 2748.330 kb on - strand, within Psest_2565 at 2748.376 kb on + strand at 2749.012 kb on - strand, within Psest_2566 at 2749.176 kb on - strand at 2749.201 kb on + strand at 2749.355 kb on + strand at 2749.355 kb on + strand at 2749.355 kb on + strand at 2749.356 kb on - strand at 2749.356 kb on - strand at 2749.356 kb on - strand at 2749.364 kb on + strand at 2749.365 kb on - strand at 2749.365 kb on - strand at 2749.365 kb on - strand at 2749.365 kb on - strand at 2749.839 kb on + strand, within Psest_2567 at 2749.840 kb on - strand, within Psest_2567 at 2749.882 kb on + strand, within Psest_2567 at 2749.883 kb on - strand, within Psest_2567 at 2749.983 kb on + strand, within Psest_2567 at 2749.984 kb on - strand, within Psest_2567 at 2749.984 kb on - strand, within Psest_2567 at 2749.984 kb on - strand, within Psest_2567
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate/ lactate media with Nitrate 20mM and .05mM Tungsten remove 2,747,637 + -0.9 2,747,637 + -1.5 2,747,637 + +0.6 2,747,637 + -1.9 2,747,637 + -0.8 2,747,637 + +1.1 2,747,638 - -0.3 2,747,638 - +0.0 2,747,638 - -0.1 2,747,638 - +0.8 2,747,638 - +0.9 2,747,638 - -0.4 2,747,638 - +1.2 2,747,638 - +0.8 2,748,071 + Psest_2565 0.57 -2.2 2,748,166 - Psest_2565 0.68 +0.9 2,748,166 - Psest_2565 0.68 +0.3 2,748,330 - Psest_2565 0.86 +0.9 2,748,376 + -0.6 2,749,012 - Psest_2566 0.87 -1.4 2,749,176 - -1.5 2,749,201 + -0.2 2,749,355 + -0.0 2,749,355 + -1.5 2,749,355 + +1.5 2,749,356 - -0.2 2,749,356 - +0.3 2,749,356 - -0.3 2,749,364 + +0.5 2,749,365 - -0.5 2,749,365 - -1.0 2,749,365 - -0.2 2,749,365 - -0.0 2,749,839 + Psest_2567 0.48 -0.2 2,749,840 - Psest_2567 0.49 +0.8 2,749,882 + Psest_2567 0.54 +0.1 2,749,883 - Psest_2567 0.54 +0.5 2,749,983 + Psest_2567 0.66 -0.2 2,749,984 - Psest_2567 0.66 +0.1 2,749,984 - Psest_2567 0.66 +2.5 2,749,984 - Psest_2567 0.66 +0.8
Or see this region's nucleotide sequence