Experiment: Nitrate/ lactate media with Nitrate 20mM and .05mM Tungsten
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2542 and Psest_2543 overlap by 4 nucleotides Psest_2543 and Psest_2544 are separated by 99 nucleotides Psest_2544 and Psest_2545 are separated by 144 nucleotides Psest_2545 and Psest_2546 are separated by 101 nucleotides
Psest_2542: Psest_2542 - ABC-type nitrate/sulfonate/bicarbonate transport system, permease component, at 2,721,805 to 2,722,578
_2542
Psest_2543: Psest_2543 - ABC-type nitrate/sulfonate/bicarbonate transport system, ATPase component, at 2,722,575 to 2,723,321
_2543
Psest_2544: Psest_2544 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 2,723,421 to 2,724,410
_2544
Psest_2545: Psest_2545 - recombination protein RecR, at 2,724,555 to 2,725,154
_2545
Psest_2546: Psest_2546 - DNA-binding protein, YbaB/EbfC family, at 2,725,256 to 2,725,582
_2546
Position (kb)
2723
2724
2725 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2722.541 kb on + strand at 2722.541 kb on + strand at 2722.542 kb on - strand at 2722.542 kb on - strand at 2722.738 kb on + strand, within Psest_2543 at 2722.738 kb on + strand, within Psest_2543 at 2722.945 kb on + strand, within Psest_2543 at 2722.948 kb on + strand, within Psest_2543 at 2722.948 kb on + strand, within Psest_2543 at 2722.948 kb on + strand, within Psest_2543 at 2722.980 kb on + strand, within Psest_2543 at 2723.074 kb on + strand, within Psest_2543 at 2723.298 kb on + strand at 2723.299 kb on - strand at 2723.299 kb on - strand at 2723.299 kb on - strand at 2723.299 kb on - strand at 2723.299 kb on - strand at 2723.299 kb on - strand at 2723.602 kb on - strand, within Psest_2544 at 2723.662 kb on + strand, within Psest_2544 at 2723.662 kb on + strand, within Psest_2544 at 2723.662 kb on + strand, within Psest_2544 at 2723.663 kb on - strand, within Psest_2544 at 2723.812 kb on - strand, within Psest_2544 at 2723.812 kb on - strand, within Psest_2544 at 2723.827 kb on + strand, within Psest_2544 at 2723.828 kb on - strand, within Psest_2544 at 2723.828 kb on - strand, within Psest_2544 at 2723.828 kb on - strand, within Psest_2544 at 2724.124 kb on - strand, within Psest_2544 at 2724.329 kb on - strand at 2724.329 kb on - strand at 2724.441 kb on - strand at 2724.490 kb on + strand at 2724.537 kb on + strand at 2724.558 kb on + strand at 2724.559 kb on - strand at 2724.559 kb on - strand at 2724.559 kb on - strand at 2724.559 kb on - strand at 2724.559 kb on - strand at 2724.694 kb on + strand, within Psest_2545 at 2724.694 kb on + strand, within Psest_2545 at 2724.695 kb on - strand, within Psest_2545 at 2724.789 kb on - strand, within Psest_2545 at 2724.848 kb on - strand, within Psest_2545 at 2724.848 kb on - strand, within Psest_2545 at 2725.096 kb on - strand at 2725.159 kb on - strand at 2725.250 kb on + strand at 2725.250 kb on + strand at 2725.257 kb on + strand at 2725.257 kb on + strand at 2725.258 kb on - strand at 2725.258 kb on - strand at 2725.258 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate/ lactate media with Nitrate 20mM and .05mM Tungsten remove 2,722,541 + -0.1 2,722,541 + -1.3 2,722,542 - -0.4 2,722,542 - -0.8 2,722,738 + Psest_2543 0.22 -0.9 2,722,738 + Psest_2543 0.22 -0.3 2,722,945 + Psest_2543 0.50 -0.8 2,722,948 + Psest_2543 0.50 +0.2 2,722,948 + Psest_2543 0.50 -0.2 2,722,948 + Psest_2543 0.50 -1.3 2,722,980 + Psest_2543 0.54 +0.5 2,723,074 + Psest_2543 0.67 +0.5 2,723,298 + -0.5 2,723,299 - -0.4 2,723,299 - +0.1 2,723,299 - +2.0 2,723,299 - -0.9 2,723,299 - -1.5 2,723,299 - -0.5 2,723,602 - Psest_2544 0.18 -2.1 2,723,662 + Psest_2544 0.24 -0.5 2,723,662 + Psest_2544 0.24 -0.8 2,723,662 + Psest_2544 0.24 -0.5 2,723,663 - Psest_2544 0.24 -0.5 2,723,812 - Psest_2544 0.39 +0.5 2,723,812 - Psest_2544 0.39 -0.5 2,723,827 + Psest_2544 0.41 -0.7 2,723,828 - Psest_2544 0.41 +0.5 2,723,828 - Psest_2544 0.41 -0.3 2,723,828 - Psest_2544 0.41 -0.6 2,724,124 - Psest_2544 0.71 +0.5 2,724,329 - -1.2 2,724,329 - -1.4 2,724,441 - -1.0 2,724,490 + -0.1 2,724,537 + -2.3 2,724,558 + +1.6 2,724,559 - -0.9 2,724,559 - +1.5 2,724,559 - -0.9 2,724,559 - +0.7 2,724,559 - +1.5 2,724,694 + Psest_2545 0.23 -0.1 2,724,694 + Psest_2545 0.23 -0.1 2,724,695 - Psest_2545 0.23 -1.5 2,724,789 - Psest_2545 0.39 +0.0 2,724,848 - Psest_2545 0.49 +0.3 2,724,848 - Psest_2545 0.49 -0.8 2,725,096 - +0.5 2,725,159 - -0.6 2,725,250 + -0.9 2,725,250 + +0.3 2,725,257 + -0.5 2,725,257 + +2.3 2,725,258 - -0.1 2,725,258 - -1.9 2,725,258 - +0.7
Or see this region's nucleotide sequence