Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_2022

Experiment: Nitrate/ lactate media with Nitrate 20mM and .05mM Tungsten

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_2021 and Psest_2022 are separated by 69 nucleotidesPsest_2022 and Psest_2023 are separated by 102 nucleotides Psest_2021: Psest_2021 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase, at 2,140,157 to 2,141,284 _2021 Psest_2022: Psest_2022 - ADP-ribose pyrophosphatase, at 2,141,354 to 2,141,794 _2022 Psest_2023: Psest_2023 - isocitrate dehydrogenase, NADP-dependent, monomeric type, at 2,141,897 to 2,144,125 _2023 Position (kb) 2141 2142Strain fitness (log2 ratio) -1 0 1at 2141.285 kb on - strandat 2141.553 kb on + strand, within Psest_2022at 2141.553 kb on + strand, within Psest_2022at 2141.580 kb on + strand, within Psest_2022at 2141.581 kb on - strand, within Psest_2022at 2141.586 kb on + strand, within Psest_2022at 2141.587 kb on - strand, within Psest_2022at 2141.587 kb on - strand, within Psest_2022at 2141.587 kb on - strand, within Psest_2022at 2141.777 kb on - strandat 2141.794 kb on + strandat 2141.795 kb on - strandat 2141.810 kb on + strandat 2141.810 kb on + strandat 2141.810 kb on + strandat 2141.811 kb on - strandat 2141.811 kb on - strandat 2142.394 kb on - strand, within Psest_2023at 2142.394 kb on - strand, within Psest_2023at 2142.481 kb on - strand, within Psest_2023at 2142.613 kb on - strand, within Psest_2023at 2142.613 kb on - strand, within Psest_2023

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate/ lactate media with Nitrate 20mM and .05mM Tungsten
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2,141,285 - -0.7
2,141,553 + Psest_2022 0.45 -0.3
2,141,553 + Psest_2022 0.45 -0.3
2,141,580 + Psest_2022 0.51 -0.7
2,141,581 - Psest_2022 0.51 -0.5
2,141,586 + Psest_2022 0.53 -0.5
2,141,587 - Psest_2022 0.53 -0.3
2,141,587 - Psest_2022 0.53 -0.1
2,141,587 - Psest_2022 0.53 +0.5
2,141,777 - +0.6
2,141,794 + -0.1
2,141,795 - +0.1
2,141,810 + -1.3
2,141,810 + +0.8
2,141,810 + +0.0
2,141,811 - -0.8
2,141,811 - -1.1
2,142,394 - Psest_2023 0.22 -0.8
2,142,394 - Psest_2023 0.22 +1.5
2,142,481 - Psest_2023 0.26 +1.5
2,142,613 - Psest_2023 0.32 -0.4
2,142,613 - Psest_2023 0.32 +1.5

Or see this region's nucleotide sequence