Experiment: Nitrate/ lactate media with Nitrate 250mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1947 and Psest_1948 are separated by 92 nucleotides Psest_1948 and Psest_1949 are separated by 356 nucleotides Psest_1949 and Psest_1950 are separated by 39 nucleotides
Psest_1947: Psest_1947 - dihydroorotate dehydrogenase, subfamily 2, at 2,063,694 to 2,064,725
_1947
Psest_1948: Psest_1948 - Ribosome modulation factor, at 2,064,818 to 2,065,033
_1948
Psest_1949: Psest_1949 - Predicted N6-adenine-specific DNA methylase, at 2,065,390 to 2,067,570
_1949
Psest_1950: Psest_1950 - D-alanyl-D-alanine carboxypeptidase, serine-type, PBP4 family, at 2,067,610 to 2,069,067
_1950
Position (kb)
2065
2066
2067
2068 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2064.465 kb on + strand, within Psest_1947 at 2064.653 kb on + strand at 2064.653 kb on + strand at 2064.657 kb on + strand at 2064.657 kb on + strand at 2064.657 kb on + strand at 2064.657 kb on + strand at 2064.658 kb on - strand at 2064.767 kb on - strand at 2064.847 kb on + strand, within Psest_1948 at 2064.848 kb on - strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.971 kb on + strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2064.972 kb on - strand, within Psest_1948 at 2065.094 kb on + strand at 2065.103 kb on + strand at 2065.103 kb on + strand at 2065.104 kb on - strand at 2065.213 kb on + strand at 2065.213 kb on + strand at 2065.213 kb on + strand at 2065.213 kb on + strand at 2065.213 kb on + strand at 2065.213 kb on + strand at 2065.213 kb on + strand at 2065.214 kb on - strand at 2065.214 kb on - strand at 2065.214 kb on - strand at 2065.214 kb on - strand at 2065.214 kb on - strand at 2065.227 kb on - strand at 2065.251 kb on + strand at 2065.251 kb on + strand at 2065.500 kb on + strand at 2065.501 kb on - strand at 2065.782 kb on + strand, within Psest_1949 at 2065.783 kb on - strand, within Psest_1949 at 2065.821 kb on - strand, within Psest_1949 at 2066.013 kb on + strand, within Psest_1949 at 2066.024 kb on + strand, within Psest_1949 at 2066.024 kb on + strand, within Psest_1949 at 2066.025 kb on - strand, within Psest_1949 at 2066.105 kb on + strand, within Psest_1949 at 2066.105 kb on + strand, within Psest_1949 at 2066.105 kb on + strand, within Psest_1949 at 2066.106 kb on - strand, within Psest_1949 at 2066.106 kb on - strand, within Psest_1949 at 2066.540 kb on + strand, within Psest_1949 at 2066.599 kb on - strand, within Psest_1949 at 2066.599 kb on - strand, within Psest_1949 at 2066.732 kb on - strand, within Psest_1949 at 2066.732 kb on - strand, within Psest_1949 at 2066.800 kb on + strand, within Psest_1949 at 2067.000 kb on - strand, within Psest_1949 at 2067.000 kb on - strand, within Psest_1949 at 2067.020 kb on - strand, within Psest_1949 at 2067.112 kb on + strand, within Psest_1949 at 2067.187 kb on + strand, within Psest_1949 at 2067.187 kb on + strand, within Psest_1949 at 2067.187 kb on + strand, within Psest_1949 at 2067.207 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.260 kb on + strand, within Psest_1949 at 2067.261 kb on - strand, within Psest_1949 at 2067.424 kb on + strand at 2067.424 kb on + strand at 2067.424 kb on + strand at 2067.424 kb on + strand at 2067.424 kb on + strand at 2067.424 kb on + strand at 2067.644 kb on + strand at 2067.645 kb on - strand at 2067.819 kb on + strand, within Psest_1950 at 2067.819 kb on + strand, within Psest_1950 at 2067.819 kb on + strand, within Psest_1950 at 2067.819 kb on + strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.820 kb on - strand, within Psest_1950 at 2067.873 kb on + strand, within Psest_1950 at 2067.874 kb on - strand, within Psest_1950 at 2067.874 kb on - strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.121 kb on + strand, within Psest_1950 at 2068.122 kb on - strand, within Psest_1950 at 2068.122 kb on - strand, within Psest_1950 at 2068.122 kb on - strand, within Psest_1950 at 2068.122 kb on - strand, within Psest_1950 at 2068.122 kb on - strand, within Psest_1950 at 2068.122 kb on - strand, within Psest_1950 at 2068.122 kb on - strand, within Psest_1950 at 2068.272 kb on - strand, within Psest_1950 at 2068.298 kb on + strand, within Psest_1950 at 2068.298 kb on + strand, within Psest_1950 at 2068.299 kb on - strand, within Psest_1950 at 2068.299 kb on - strand, within Psest_1950 at 2068.343 kb on - strand, within Psest_1950 at 2068.376 kb on + strand, within Psest_1950 at 2068.376 kb on + strand, within Psest_1950 at 2068.376 kb on + strand, within Psest_1950 at 2068.376 kb on + strand, within Psest_1950 at 2068.376 kb on + strand, within Psest_1950 at 2068.376 kb on + strand, within Psest_1950 at 2068.377 kb on - strand, within Psest_1950 at 2068.377 kb on - strand, within Psest_1950 at 2068.377 kb on - strand, within Psest_1950 at 2068.377 kb on - strand, within Psest_1950 at 2068.377 kb on - strand, within Psest_1950 at 2068.381 kb on + strand, within Psest_1950 at 2068.381 kb on + strand, within Psest_1950 at 2068.381 kb on + strand, within Psest_1950 at 2068.381 kb on + strand, within Psest_1950 at 2068.382 kb on - strand, within Psest_1950 at 2068.382 kb on - strand, within Psest_1950 at 2068.382 kb on - strand, within Psest_1950 at 2068.382 kb on - strand, within Psest_1950 at 2068.423 kb on + strand, within Psest_1950
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate/ lactate media with Nitrate 250mM remove 2,064,465 + Psest_1947 0.75 +1.6 2,064,653 + +1.6 2,064,653 + +1.6 2,064,657 + +1.6 2,064,657 + +0.6 2,064,657 + +1.6 2,064,657 + +1.6 2,064,658 - +1.6 2,064,767 - -0.7 2,064,847 + Psest_1948 0.13 +0.6 2,064,848 - Psest_1948 0.14 -1.4 2,064,971 + Psest_1948 0.71 -0.3 2,064,971 + Psest_1948 0.71 -0.6 2,064,971 + Psest_1948 0.71 +0.2 2,064,971 + Psest_1948 0.71 -1.1 2,064,971 + Psest_1948 0.71 +1.6 2,064,971 + Psest_1948 0.71 +0.4 2,064,971 + Psest_1948 0.71 +0.6 2,064,971 + Psest_1948 0.71 -1.6 2,064,971 + Psest_1948 0.71 +1.6 2,064,971 + Psest_1948 0.71 -0.1 2,064,971 + Psest_1948 0.71 +0.1 2,064,971 + Psest_1948 0.71 -0.0 2,064,971 + Psest_1948 0.71 +1.1 2,064,971 + Psest_1948 0.71 -1.2 2,064,971 + Psest_1948 0.71 -0.2 2,064,971 + Psest_1948 0.71 -0.2 2,064,971 + Psest_1948 0.71 +0.2 2,064,972 - Psest_1948 0.71 +0.1 2,064,972 - Psest_1948 0.71 -0.1 2,064,972 - Psest_1948 0.71 +0.1 2,064,972 - Psest_1948 0.71 -1.1 2,064,972 - Psest_1948 0.71 -0.1 2,064,972 - Psest_1948 0.71 -2.3 2,064,972 - Psest_1948 0.71 -0.3 2,064,972 - Psest_1948 0.71 +1.1 2,064,972 - Psest_1948 0.71 +0.5 2,064,972 - Psest_1948 0.71 +0.3 2,064,972 - Psest_1948 0.71 -0.9 2,064,972 - Psest_1948 0.71 +0.3 2,065,094 + +0.2 2,065,103 + -0.5 2,065,103 + -1.0 2,065,104 - -0.7 2,065,213 + +0.6 2,065,213 + -0.6 2,065,213 + +1.1 2,065,213 + +0.2 2,065,213 + +0.3 2,065,213 + +1.2 2,065,213 + +0.6 2,065,214 - -1.8 2,065,214 - -0.2 2,065,214 - +0.6 2,065,214 - +0.2 2,065,214 - -0.1 2,065,227 - -0.7 2,065,251 + -0.0 2,065,251 + +0.3 2,065,500 + +0.6 2,065,501 - -1.1 2,065,782 + Psest_1949 0.18 -0.7 2,065,783 - Psest_1949 0.18 -0.5 2,065,821 - Psest_1949 0.20 -1.5 2,066,013 + Psest_1949 0.29 -1.1 2,066,024 + Psest_1949 0.29 +0.6 2,066,024 + Psest_1949 0.29 +2.1 2,066,025 - Psest_1949 0.29 -0.4 2,066,105 + Psest_1949 0.33 -0.8 2,066,105 + Psest_1949 0.33 +3.2 2,066,105 + Psest_1949 0.33 -0.5 2,066,106 - Psest_1949 0.33 +0.1 2,066,106 - Psest_1949 0.33 -0.2 2,066,540 + Psest_1949 0.53 -0.6 2,066,599 - Psest_1949 0.55 -0.4 2,066,599 - Psest_1949 0.55 -1.5 2,066,732 - Psest_1949 0.62 -0.7 2,066,732 - Psest_1949 0.62 -0.1 2,066,800 + Psest_1949 0.65 -0.7 2,067,000 - Psest_1949 0.74 -0.4 2,067,000 - Psest_1949 0.74 -1.4 2,067,020 - Psest_1949 0.75 -0.8 2,067,112 + Psest_1949 0.79 -0.4 2,067,187 + Psest_1949 0.82 -0.9 2,067,187 + Psest_1949 0.82 +0.1 2,067,187 + Psest_1949 0.82 -0.0 2,067,207 + Psest_1949 0.83 -0.1 2,067,260 + Psest_1949 0.86 -0.4 2,067,260 + Psest_1949 0.86 +0.2 2,067,260 + Psest_1949 0.86 -0.3 2,067,260 + Psest_1949 0.86 +0.6 2,067,260 + Psest_1949 0.86 -0.6 2,067,260 + Psest_1949 0.86 -0.2 2,067,260 + Psest_1949 0.86 +0.3 2,067,260 + Psest_1949 0.86 -0.6 2,067,261 - Psest_1949 0.86 +1.6 2,067,424 + -0.4 2,067,424 + -1.2 2,067,424 + -0.7 2,067,424 + +0.4 2,067,424 + -2.7 2,067,424 + -1.8 2,067,644 + +0.6 2,067,645 - +0.4 2,067,819 + Psest_1950 0.14 -2.3 2,067,819 + Psest_1950 0.14 -0.9 2,067,819 + Psest_1950 0.14 +0.8 2,067,819 + Psest_1950 0.14 +1.6 2,067,820 - Psest_1950 0.14 +0.0 2,067,820 - Psest_1950 0.14 -1.3 2,067,820 - Psest_1950 0.14 +0.1 2,067,820 - Psest_1950 0.14 -0.1 2,067,820 - Psest_1950 0.14 -0.8 2,067,820 - Psest_1950 0.14 -0.9 2,067,820 - Psest_1950 0.14 +0.1 2,067,820 - Psest_1950 0.14 +0.6 2,067,820 - Psest_1950 0.14 -1.4 2,067,820 - Psest_1950 0.14 +1.6 2,067,873 + Psest_1950 0.18 -0.1 2,067,874 - Psest_1950 0.18 +1.1 2,067,874 - Psest_1950 0.18 -0.4 2,068,121 + Psest_1950 0.35 -0.1 2,068,121 + Psest_1950 0.35 -0.1 2,068,121 + Psest_1950 0.35 -0.5 2,068,121 + Psest_1950 0.35 -1.2 2,068,121 + Psest_1950 0.35 -1.5 2,068,121 + Psest_1950 0.35 -0.5 2,068,121 + Psest_1950 0.35 +0.3 2,068,121 + Psest_1950 0.35 -1.0 2,068,122 - Psest_1950 0.35 -2.4 2,068,122 - Psest_1950 0.35 -0.7 2,068,122 - Psest_1950 0.35 +0.1 2,068,122 - Psest_1950 0.35 -0.2 2,068,122 - Psest_1950 0.35 -0.8 2,068,122 - Psest_1950 0.35 +0.4 2,068,122 - Psest_1950 0.35 -0.5 2,068,272 - Psest_1950 0.45 +0.6 2,068,298 + Psest_1950 0.47 -0.9 2,068,298 + Psest_1950 0.47 +1.1 2,068,299 - Psest_1950 0.47 +0.6 2,068,299 - Psest_1950 0.47 -0.5 2,068,343 - Psest_1950 0.50 -1.7 2,068,376 + Psest_1950 0.53 +0.3 2,068,376 + Psest_1950 0.53 -0.0 2,068,376 + Psest_1950 0.53 -2.4 2,068,376 + Psest_1950 0.53 -1.4 2,068,376 + Psest_1950 0.53 -0.9 2,068,376 + Psest_1950 0.53 +0.1 2,068,377 - Psest_1950 0.53 -2.0 2,068,377 - Psest_1950 0.53 -0.2 2,068,377 - Psest_1950 0.53 +0.3 2,068,377 - Psest_1950 0.53 -0.9 2,068,377 - Psest_1950 0.53 -0.4 2,068,381 + Psest_1950 0.53 +0.1 2,068,381 + Psest_1950 0.53 +1.2 2,068,381 + Psest_1950 0.53 -0.6 2,068,381 + Psest_1950 0.53 +1.6 2,068,382 - Psest_1950 0.53 -0.7 2,068,382 - Psest_1950 0.53 +0.1 2,068,382 - Psest_1950 0.53 +0.6 2,068,382 - Psest_1950 0.53 +0.3 2,068,423 + Psest_1950 0.56 +0.6
Or see this region's nucleotide sequence