Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1753

Experiment: Nitrate/ lactate media with Nitrate 250mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1750 and Psest_1751 are separated by 134 nucleotidesPsest_1751 and Psest_1752 overlap by 20 nucleotidesPsest_1752 and Psest_1753 overlap by 4 nucleotidesPsest_1753 and Psest_1754 are separated by 135 nucleotidesPsest_1754 and Psest_1755 overlap by 4 nucleotides Psest_1750: Psest_1750 - Protein of unknown function (DUF2802)., at 1,854,588 to 1,855,001 _1750 Psest_1751: Psest_1751 - Rhodanese-related sulfurtransferase, at 1,855,136 to 1,855,579 _1751 Psest_1752: Psest_1752 - Uncharacterized homolog of the cytoplasmic domain of flagellar protein FhlB, at 1,855,560 to 1,855,892 _1752 Psest_1753: Psest_1753 - Flagellar hook-length control protein FliK., at 1,855,889 to 1,857,418 _1753 Psest_1754: Psest_1754 - heme ABC exporter, ATP-binding protein CcmA, at 1,857,554 to 1,858,189 _1754 Psest_1755: Psest_1755 - heme exporter protein CcmB, at 1,858,186 to 1,858,857 _1755 Position (kb) 1855 1856 1857 1858Strain fitness (log2 ratio) -2 -1 0 1at 1854.911 kb on + strand, within Psest_1750at 1855.186 kb on - strand, within Psest_1751at 1855.403 kb on + strand, within Psest_1751at 1855.403 kb on + strand, within Psest_1751at 1855.403 kb on + strand, within Psest_1751at 1855.404 kb on - strand, within Psest_1751at 1855.404 kb on - strand, within Psest_1751at 1855.419 kb on + strand, within Psest_1751at 1855.419 kb on + strand, within Psest_1751at 1855.419 kb on + strand, within Psest_1751at 1855.419 kb on + strand, within Psest_1751at 1855.419 kb on + strand, within Psest_1751at 1855.419 kb on + strand, within Psest_1751at 1855.420 kb on - strand, within Psest_1751at 1855.420 kb on - strand, within Psest_1751at 1855.420 kb on - strand, within Psest_1751at 1855.467 kb on - strand, within Psest_1751at 1855.481 kb on + strand, within Psest_1751at 1855.482 kb on - strand, within Psest_1751at 1855.496 kb on + strand, within Psest_1751at 1855.497 kb on - strand, within Psest_1751at 1855.512 kb on + strand, within Psest_1751at 1855.513 kb on - strand, within Psest_1751at 1855.652 kb on - strand, within Psest_1752at 1855.714 kb on - strand, within Psest_1752at 1856.263 kb on + strand, within Psest_1753at 1856.264 kb on - strand, within Psest_1753at 1856.585 kb on + strand, within Psest_1753at 1856.585 kb on + strand, within Psest_1753at 1856.639 kb on + strand, within Psest_1753at 1856.640 kb on - strand, within Psest_1753at 1857.159 kb on + strand, within Psest_1753at 1857.159 kb on + strand, within Psest_1753at 1857.159 kb on + strand, within Psest_1753at 1857.160 kb on - strand, within Psest_1753at 1857.436 kb on + strandat 1857.437 kb on - strandat 1857.437 kb on - strandat 1857.481 kb on + strandat 1857.481 kb on + strandat 1857.481 kb on + strandat 1857.481 kb on + strandat 1857.481 kb on + strandat 1857.482 kb on - strandat 1857.482 kb on - strandat 1857.482 kb on - strandat 1857.482 kb on - strandat 1857.482 kb on - strandat 1857.483 kb on + strandat 1857.514 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate/ lactate media with Nitrate 250mM
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1,854,911 + Psest_1750 0.78 -1.4
1,855,186 - Psest_1751 0.11 +1.1
1,855,403 + Psest_1751 0.60 +0.2
1,855,403 + Psest_1751 0.60 +0.9
1,855,403 + Psest_1751 0.60 -0.4
1,855,404 - Psest_1751 0.60 +0.2
1,855,404 - Psest_1751 0.60 +0.2
1,855,419 + Psest_1751 0.64 +0.5
1,855,419 + Psest_1751 0.64 +0.6
1,855,419 + Psest_1751 0.64 +0.9
1,855,419 + Psest_1751 0.64 +1.6
1,855,419 + Psest_1751 0.64 +0.6
1,855,419 + Psest_1751 0.64 -0.2
1,855,420 - Psest_1751 0.64 +1.6
1,855,420 - Psest_1751 0.64 -0.9
1,855,420 - Psest_1751 0.64 +0.9
1,855,467 - Psest_1751 0.75 -0.7
1,855,481 + Psest_1751 0.78 -0.8
1,855,482 - Psest_1751 0.78 -0.7
1,855,496 + Psest_1751 0.81 +0.2
1,855,497 - Psest_1751 0.81 -1.2
1,855,512 + Psest_1751 0.85 -0.4
1,855,513 - Psest_1751 0.85 +0.6
1,855,652 - Psest_1752 0.28 -0.2
1,855,714 - Psest_1752 0.46 +1.6
1,856,263 + Psest_1753 0.24 +0.2
1,856,264 - Psest_1753 0.25 +0.7
1,856,585 + Psest_1753 0.45 +1.6
1,856,585 + Psest_1753 0.45 +0.6
1,856,639 + Psest_1753 0.49 +0.1
1,856,640 - Psest_1753 0.49 -1.8
1,857,159 + Psest_1753 0.83 -0.1
1,857,159 + Psest_1753 0.83 -0.5
1,857,159 + Psest_1753 0.83 -0.9
1,857,160 - Psest_1753 0.83 +0.4
1,857,436 + +1.2
1,857,437 - +0.6
1,857,437 - +0.2
1,857,481 + +1.1
1,857,481 + -0.4
1,857,481 + +0.7
1,857,481 + -0.9
1,857,481 + -0.6
1,857,482 - +1.1
1,857,482 - +1.6
1,857,482 - -1.7
1,857,482 - +0.6
1,857,482 - -0.4
1,857,483 + -0.9
1,857,514 + -0.7

Or see this region's nucleotide sequence