Experiment: Nitrate/ lactate media with Nitrate 250mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0160 and Psest_0161 are separated by 71 nucleotides Psest_0161 and Psest_0162 are separated by 114 nucleotides
Psest_0160: Psest_0160 - C-terminal peptidase (prc), at 157,851 to 159,170
_0160
Psest_0161: Psest_0161 - Membrane-bound metallopeptidase, at 159,242 to 160,504
_0161
Psest_0162: Psest_0162 - 2,3-bisphosphoglycerate-independent phosphoglycerate mutase, at 160,619 to 162,166
_0162
Position (kb)
159
160
161 Strain fitness (log2 ratio)
-1
0
1
2 at 159.184 kb on + strand at 159.307 kb on - strand at 159.342 kb on - strand at 159.412 kb on - strand, within Psest_0161 at 159.482 kb on + strand, within Psest_0161 at 159.482 kb on + strand, within Psest_0161 at 159.483 kb on - strand, within Psest_0161 at 159.540 kb on - strand, within Psest_0161 at 160.086 kb on + strand, within Psest_0161 at 160.527 kb on - strand at 160.537 kb on + strand at 160.538 kb on - strand at 160.538 kb on - strand at 160.538 kb on - strand at 160.538 kb on - strand at 160.538 kb on - strand at 160.538 kb on - strand at 160.538 kb on - strand at 160.538 kb on - strand at 160.538 kb on - strand at 160.538 kb on - strand at 160.538 kb on - strand at 160.538 kb on - strand at 160.538 kb on - strand at 160.538 kb on - strand at 160.538 kb on - strand at 160.538 kb on - strand at 160.541 kb on - strand at 160.541 kb on - strand at 160.541 kb on - strand at 160.541 kb on - strand at 160.541 kb on - strand at 160.541 kb on - strand at 160.541 kb on - strand at 160.541 kb on - strand at 160.541 kb on - strand at 160.547 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate/ lactate media with Nitrate 250mM remove 159,184 + +1.4 159,307 - +0.3 159,342 - +0.6 159,412 - Psest_0161 0.13 -0.5 159,482 + Psest_0161 0.19 +0.3 159,482 + Psest_0161 0.19 -0.7 159,483 - Psest_0161 0.19 -1.0 159,540 - Psest_0161 0.24 +1.1 160,086 + Psest_0161 0.67 -0.3 160,527 - +0.4 160,537 + -0.2 160,538 - +1.3 160,538 - +0.1 160,538 - +1.8 160,538 - +1.1 160,538 - -1.4 160,538 - +1.3 160,538 - -0.7 160,538 - -1.1 160,538 - -0.8 160,538 - +1.0 160,538 - -0.0 160,538 - +0.1 160,538 - +0.8 160,538 - +0.4 160,538 - -0.5 160,538 - +0.3 160,541 - +1.6 160,541 - -1.0 160,541 - -0.2 160,541 - +0.2 160,541 - -0.3 160,541 - -0.2 160,541 - +1.3 160,541 - +0.5 160,541 - -0.6 160,547 - +0.3
Or see this region's nucleotide sequence