Experiment: Nitrate/ lactate media with Nitrate 50mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1104 and Psest_1105 are separated by 33 nucleotides Psest_1105 and Psest_1106 are separated by 119 nucleotides Psest_1106 and Psest_1107 are separated by 30 nucleotides Psest_1107 and Psest_1108 are separated by 58 nucleotides Psest_1108 and Psest_1109 are separated by 19 nucleotides
Psest_1104: Psest_1104 - peptidyl-tRNA hydrolase, at 1,200,537 to 1,201,121
_1104
Psest_1105: Psest_1105 - ribosomal protein L25, Ctc-form, at 1,201,155 to 1,201,772
_1105
Psest_1106: Psest_1106 - ribose-phosphate pyrophosphokinase, at 1,201,892 to 1,202,881
_1106
Psest_1107: Psest_1107 - tRNA_Lys_TTT, at 1,202,912 to 1,202,987
_1107
Psest_1108: Psest_1108 - tRNA_Gln_TTG, at 1,203,046 to 1,203,120
_1108
Psest_1109: Psest_1109 - 4-diphosphocytidyl-2C-methyl-D-erythritol kinase, at 1,203,140 to 1,204,000
_1109
Position (kb)
1201
1202
1203 Strain fitness (log2 ratio)
-1
0
1 at 1201.863 kb on - strand at 1203.003 kb on - strand at 1203.003 kb on - strand at 1203.003 kb on - strand at 1203.003 kb on - strand at 1203.003 kb on - strand at 1203.028 kb on - strand at 1203.028 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate/ lactate media with Nitrate 50mM remove 1,201,863 - -0.7 1,203,003 - -0.5 1,203,003 - +0.7 1,203,003 - +1.3 1,203,003 - -0.7 1,203,003 - +0.3 1,203,028 - +1.3 1,203,028 - +1.3
Or see this region's nucleotide sequence