Experiment: Nitrate/ lactate media with Nitrate 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3076 and Psest_3077 are separated by 228 nucleotides Psest_3077 and Psest_3078 are separated by 113 nucleotides
Psest_3076: Psest_3076 - Outer membrane receptor proteins, mostly Fe transport, at 3,252,934 to 3,254,949
_3076
Psest_3077: Psest_3077 - Protein of unknown function (DUF3203)., at 3,255,178 to 3,255,402
_3077
Psest_3078: Psest_3078 - L-proline:Na+ symporter (from data) , at 3,255,516 to 3,257,000
_3078
Position (kb)
3255
3256 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3254.225 kb on + strand, within Psest_3076 at 3254.226 kb on - strand, within Psest_3076 at 3254.226 kb on - strand, within Psest_3076 at 3254.240 kb on + strand, within Psest_3076 at 3254.240 kb on + strand, within Psest_3076 at 3254.294 kb on + strand, within Psest_3076 at 3254.294 kb on + strand, within Psest_3076 at 3254.295 kb on - strand, within Psest_3076 at 3254.460 kb on - strand, within Psest_3076 at 3254.534 kb on + strand, within Psest_3076 at 3255.082 kb on - strand at 3255.086 kb on + strand at 3255.086 kb on + strand at 3255.087 kb on - strand at 3255.087 kb on - strand at 3255.087 kb on - strand at 3255.186 kb on - strand at 3255.186 kb on - strand at 3255.234 kb on + strand, within Psest_3077 at 3255.234 kb on + strand, within Psest_3077 at 3255.234 kb on + strand, within Psest_3077 at 3255.235 kb on - strand, within Psest_3077 at 3255.235 kb on - strand, within Psest_3077 at 3255.606 kb on - strand at 3255.929 kb on - strand, within Psest_3078 at 3255.929 kb on - strand, within Psest_3078 at 3255.929 kb on - strand, within Psest_3078 at 3255.929 kb on - strand, within Psest_3078 at 3255.929 kb on - strand, within Psest_3078 at 3255.981 kb on - strand, within Psest_3078 at 3256.117 kb on + strand, within Psest_3078 at 3256.117 kb on + strand, within Psest_3078
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate/ lactate media with Nitrate 20mM remove 3,254,225 + Psest_3076 0.64 +2.5 3,254,226 - Psest_3076 0.64 -0.3 3,254,226 - Psest_3076 0.64 +1.0 3,254,240 + Psest_3076 0.65 +0.1 3,254,240 + Psest_3076 0.65 -1.2 3,254,294 + Psest_3076 0.67 +0.0 3,254,294 + Psest_3076 0.67 +0.2 3,254,295 - Psest_3076 0.68 -0.4 3,254,460 - Psest_3076 0.76 -0.5 3,254,534 + Psest_3076 0.79 +0.5 3,255,082 - +0.3 3,255,086 + +0.2 3,255,086 + -0.2 3,255,087 - -0.9 3,255,087 - -1.2 3,255,087 - -2.6 3,255,186 - +0.9 3,255,186 - +0.6 3,255,234 + Psest_3077 0.25 +0.0 3,255,234 + Psest_3077 0.25 -2.1 3,255,234 + Psest_3077 0.25 -0.6 3,255,235 - Psest_3077 0.25 -0.9 3,255,235 - Psest_3077 0.25 -0.1 3,255,606 - +1.0 3,255,929 - Psest_3078 0.28 +0.8 3,255,929 - Psest_3078 0.28 +0.1 3,255,929 - Psest_3078 0.28 -0.3 3,255,929 - Psest_3078 0.28 +1.5 3,255,929 - Psest_3078 0.28 +1.5 3,255,981 - Psest_3078 0.31 -0.4 3,256,117 + Psest_3078 0.40 -0.3 3,256,117 + Psest_3078 0.40 -0.0
Or see this region's nucleotide sequence