Experiment: Nitrate/ lactate media with Nitrate 20mM and .05mM Tungsten
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2386 and Psest_2387 overlap by 1 nucleotides Psest_2387 and Psest_2388 are separated by 8 nucleotides Psest_2388 and Psest_2389 overlap by 4 nucleotides Psest_2389 and Psest_2390 overlap by 4 nucleotides Psest_2390 and Psest_2391 are separated by 85 nucleotides
Psest_2386: Psest_2386 - GTP cyclohydrolase I, at 2,539,131 to 2,539,688
_2386
Psest_2387: Psest_2387 - FolB domain, at 2,539,688 to 2,540,059
_2387
Psest_2388: Psest_2388 - hypothetical protein, at 2,540,068 to 2,540,589
_2388
Psest_2389: Psest_2389 - Na+/melibiose symporter and related transporters, at 2,540,586 to 2,541,824
_2389
Psest_2390: Psest_2390 - hypothetical protein, at 2,541,821 to 2,542,348
_2390
Psest_2391: Psest_2391 - PAS domain S-box, at 2,542,434 to 2,542,880
_2391
Position (kb)
2540
2541
2542 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2539.748 kb on + strand, within Psest_2387 at 2539.748 kb on + strand, within Psest_2387 at 2539.748 kb on + strand, within Psest_2387 at 2539.748 kb on + strand, within Psest_2387 at 2539.748 kb on + strand, within Psest_2387 at 2539.748 kb on + strand, within Psest_2387 at 2539.748 kb on + strand, within Psest_2387 at 2539.748 kb on + strand, within Psest_2387 at 2539.748 kb on + strand, within Psest_2387 at 2539.748 kb on + strand, within Psest_2387 at 2539.748 kb on + strand, within Psest_2387 at 2539.748 kb on + strand, within Psest_2387 at 2539.748 kb on + strand, within Psest_2387 at 2539.748 kb on + strand, within Psest_2387 at 2539.748 kb on + strand, within Psest_2387 at 2539.748 kb on + strand, within Psest_2387 at 2539.748 kb on + strand, within Psest_2387 at 2539.748 kb on + strand, within Psest_2387 at 2539.748 kb on + strand, within Psest_2387 at 2539.748 kb on + strand, within Psest_2387 at 2539.749 kb on - strand, within Psest_2387 at 2539.749 kb on - strand, within Psest_2387 at 2539.749 kb on - strand, within Psest_2387 at 2539.749 kb on - strand, within Psest_2387 at 2539.749 kb on - strand, within Psest_2387 at 2539.749 kb on - strand, within Psest_2387 at 2539.749 kb on - strand, within Psest_2387 at 2539.749 kb on - strand, within Psest_2387 at 2539.749 kb on - strand, within Psest_2387 at 2539.749 kb on - strand, within Psest_2387 at 2539.749 kb on - strand, within Psest_2387 at 2539.749 kb on - strand, within Psest_2387 at 2539.749 kb on - strand, within Psest_2387 at 2539.749 kb on - strand, within Psest_2387 at 2539.749 kb on - strand, within Psest_2387 at 2539.749 kb on - strand, within Psest_2387 at 2539.749 kb on - strand, within Psest_2387 at 2539.749 kb on - strand, within Psest_2387 at 2539.749 kb on - strand, within Psest_2387 at 2539.749 kb on - strand, within Psest_2387 at 2539.749 kb on - strand, within Psest_2387 at 2539.750 kb on + strand, within Psest_2387 at 2539.751 kb on - strand, within Psest_2387 at 2539.751 kb on - strand, within Psest_2387 at 2539.751 kb on - strand, within Psest_2387 at 2539.752 kb on + strand, within Psest_2387 at 2539.752 kb on + strand, within Psest_2387 at 2539.752 kb on + strand, within Psest_2387 at 2539.752 kb on + strand, within Psest_2387 at 2539.752 kb on + strand, within Psest_2387 at 2539.752 kb on + strand, within Psest_2387 at 2539.752 kb on + strand, within Psest_2387 at 2539.752 kb on + strand, within Psest_2387 at 2539.752 kb on + strand, within Psest_2387 at 2539.752 kb on + strand, within Psest_2387 at 2539.752 kb on + strand, within Psest_2387 at 2539.752 kb on + strand, within Psest_2387 at 2539.752 kb on + strand, within Psest_2387 at 2539.752 kb on + strand, within Psest_2387 at 2539.752 kb on + strand, within Psest_2387 at 2539.752 kb on + strand, within Psest_2387 at 2539.752 kb on + strand, within Psest_2387 at 2539.752 kb on + strand, within Psest_2387 at 2539.752 kb on + strand, within Psest_2387 at 2539.752 kb on + strand, within Psest_2387 at 2539.753 kb on - strand, within Psest_2387 at 2539.753 kb on - strand, within Psest_2387 at 2539.753 kb on - strand, within Psest_2387 at 2539.753 kb on - strand, within Psest_2387 at 2539.753 kb on - strand, within Psest_2387 at 2539.753 kb on - strand, within Psest_2387 at 2539.753 kb on - strand, within Psest_2387 at 2539.753 kb on - strand, within Psest_2387 at 2539.753 kb on - strand, within Psest_2387 at 2539.753 kb on - strand, within Psest_2387 at 2539.791 kb on + strand, within Psest_2387 at 2539.791 kb on + strand, within Psest_2387 at 2539.791 kb on + strand, within Psest_2387 at 2539.791 kb on + strand, within Psest_2387 at 2539.791 kb on + strand, within Psest_2387 at 2539.791 kb on + strand, within Psest_2387 at 2539.791 kb on + strand, within Psest_2387 at 2539.792 kb on - strand, within Psest_2387 at 2539.792 kb on - strand, within Psest_2387 at 2539.792 kb on - strand, within Psest_2387 at 2539.792 kb on - strand, within Psest_2387 at 2539.792 kb on - strand, within Psest_2387 at 2539.792 kb on - strand, within Psest_2387 at 2539.956 kb on + strand, within Psest_2387 at 2539.980 kb on - strand, within Psest_2387 at 2540.106 kb on - strand at 2540.106 kb on - strand at 2540.206 kb on + strand, within Psest_2388 at 2540.206 kb on + strand, within Psest_2388 at 2540.206 kb on + strand, within Psest_2388 at 2540.206 kb on + strand, within Psest_2388 at 2540.206 kb on + strand, within Psest_2388 at 2540.206 kb on + strand, within Psest_2388 at 2540.206 kb on + strand, within Psest_2388 at 2540.206 kb on + strand, within Psest_2388 at 2540.206 kb on + strand, within Psest_2388 at 2540.206 kb on + strand, within Psest_2388 at 2540.206 kb on + strand, within Psest_2388 at 2540.206 kb on + strand, within Psest_2388 at 2540.207 kb on - strand, within Psest_2388 at 2540.207 kb on - strand, within Psest_2388 at 2540.207 kb on - strand, within Psest_2388 at 2540.207 kb on - strand, within Psest_2388 at 2540.207 kb on - strand, within Psest_2388 at 2540.207 kb on - strand, within Psest_2388 at 2540.207 kb on - strand, within Psest_2388 at 2540.207 kb on - strand, within Psest_2388 at 2540.275 kb on + strand, within Psest_2388 at 2540.276 kb on - strand, within Psest_2388 at 2540.464 kb on + strand, within Psest_2388 at 2540.465 kb on - strand, within Psest_2388 at 2540.465 kb on - strand, within Psest_2388 at 2540.465 kb on - strand, within Psest_2388 at 2540.493 kb on - strand, within Psest_2388 at 2540.493 kb on - strand, within Psest_2388 at 2540.568 kb on + strand at 2540.568 kb on + strand at 2540.568 kb on + strand at 2540.568 kb on + strand at 2540.569 kb on - strand at 2540.569 kb on - strand at 2540.569 kb on - strand at 2540.569 kb on - strand at 2540.655 kb on + strand at 2540.655 kb on + strand at 2540.655 kb on + strand at 2540.655 kb on + strand at 2540.925 kb on + strand, within Psest_2389 at 2540.925 kb on + strand, within Psest_2389 at 2540.925 kb on + strand, within Psest_2389 at 2540.925 kb on + strand, within Psest_2389 at 2540.925 kb on + strand, within Psest_2389 at 2540.925 kb on + strand, within Psest_2389 at 2540.926 kb on - strand, within Psest_2389 at 2540.926 kb on - strand, within Psest_2389 at 2540.926 kb on - strand, within Psest_2389 at 2540.955 kb on + strand, within Psest_2389 at 2540.956 kb on - strand, within Psest_2389 at 2540.956 kb on - strand, within Psest_2389 at 2541.085 kb on - strand, within Psest_2389 at 2541.258 kb on + strand, within Psest_2389 at 2541.258 kb on + strand, within Psest_2389 at 2541.363 kb on + strand, within Psest_2389 at 2541.363 kb on + strand, within Psest_2389 at 2541.363 kb on + strand, within Psest_2389 at 2541.363 kb on + strand, within Psest_2389 at 2541.363 kb on + strand, within Psest_2389 at 2541.364 kb on - strand, within Psest_2389 at 2541.364 kb on - strand, within Psest_2389 at 2541.364 kb on - strand, within Psest_2389 at 2541.364 kb on - strand, within Psest_2389 at 2541.364 kb on - strand, within Psest_2389 at 2541.364 kb on - strand, within Psest_2389 at 2541.453 kb on - strand, within Psest_2389 at 2541.621 kb on + strand, within Psest_2389 at 2541.622 kb on - strand, within Psest_2389 at 2541.744 kb on + strand at 2541.838 kb on - strand at 2541.838 kb on - strand at 2541.922 kb on + strand, within Psest_2390 at 2541.922 kb on + strand, within Psest_2390 at 2541.926 kb on + strand, within Psest_2390 at 2541.927 kb on - strand, within Psest_2390 at 2541.927 kb on - strand, within Psest_2390 at 2541.927 kb on - strand, within Psest_2390 at 2541.927 kb on - strand, within Psest_2390 at 2541.927 kb on - strand, within Psest_2390 at 2541.927 kb on - strand, within Psest_2390 at 2541.927 kb on - strand, within Psest_2390 at 2541.927 kb on - strand, within Psest_2390 at 2541.927 kb on - strand, within Psest_2390 at 2542.220 kb on + strand, within Psest_2390 at 2542.221 kb on - strand, within Psest_2390 at 2542.247 kb on + strand, within Psest_2390 at 2542.247 kb on + strand, within Psest_2390 at 2542.247 kb on + strand, within Psest_2390 at 2542.248 kb on - strand, within Psest_2390 at 2542.248 kb on - strand, within Psest_2390 at 2542.248 kb on - strand, within Psest_2390 at 2542.248 kb on - strand, within Psest_2390 at 2542.248 kb on - strand, within Psest_2390 at 2542.248 kb on - strand, within Psest_2390 at 2542.398 kb on + strand at 2542.527 kb on + strand, within Psest_2391 at 2542.528 kb on - strand, within Psest_2391 at 2542.689 kb on + strand, within Psest_2391 at 2542.689 kb on + strand, within Psest_2391 at 2542.689 kb on + strand, within Psest_2391 at 2542.690 kb on - strand, within Psest_2391 at 2542.764 kb on + strand, within Psest_2391 at 2542.764 kb on + strand, within Psest_2391 at 2542.764 kb on + strand, within Psest_2391 at 2542.765 kb on - strand, within Psest_2391 at 2542.765 kb on - strand, within Psest_2391
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate/ lactate media with Nitrate 20mM and .05mM Tungsten remove 2,539,748 + Psest_2387 0.16 -0.5 2,539,748 + Psest_2387 0.16 -0.9 2,539,748 + Psest_2387 0.16 -0.0 2,539,748 + Psest_2387 0.16 +0.4 2,539,748 + Psest_2387 0.16 +0.2 2,539,748 + Psest_2387 0.16 +1.0 2,539,748 + Psest_2387 0.16 +2.6 2,539,748 + Psest_2387 0.16 +2.0 2,539,748 + Psest_2387 0.16 -0.4 2,539,748 + Psest_2387 0.16 +0.3 2,539,748 + Psest_2387 0.16 -0.2 2,539,748 + Psest_2387 0.16 -0.0 2,539,748 + Psest_2387 0.16 -0.2 2,539,748 + Psest_2387 0.16 +2.6 2,539,748 + Psest_2387 0.16 +1.0 2,539,748 + Psest_2387 0.16 -0.5 2,539,748 + Psest_2387 0.16 -0.1 2,539,748 + Psest_2387 0.16 -0.3 2,539,748 + Psest_2387 0.16 +1.6 2,539,748 + Psest_2387 0.16 +0.3 2,539,749 - Psest_2387 0.16 -1.6 2,539,749 - Psest_2387 0.16 +0.2 2,539,749 - Psest_2387 0.16 +0.2 2,539,749 - Psest_2387 0.16 -0.2 2,539,749 - Psest_2387 0.16 +1.4 2,539,749 - Psest_2387 0.16 -0.7 2,539,749 - Psest_2387 0.16 -0.1 2,539,749 - Psest_2387 0.16 -0.1 2,539,749 - Psest_2387 0.16 +1.4 2,539,749 - Psest_2387 0.16 +0.2 2,539,749 - Psest_2387 0.16 -2.4 2,539,749 - Psest_2387 0.16 +0.4 2,539,749 - Psest_2387 0.16 -1.6 2,539,749 - Psest_2387 0.16 -1.0 2,539,749 - Psest_2387 0.16 -0.9 2,539,749 - Psest_2387 0.16 +0.4 2,539,749 - Psest_2387 0.16 -0.6 2,539,749 - Psest_2387 0.16 -1.2 2,539,749 - Psest_2387 0.16 -0.6 2,539,749 - Psest_2387 0.16 -0.8 2,539,749 - Psest_2387 0.16 -0.2 2,539,750 + Psest_2387 0.17 +2.4 2,539,751 - Psest_2387 0.17 -0.8 2,539,751 - Psest_2387 0.17 +0.3 2,539,751 - Psest_2387 0.17 +0.8 2,539,752 + Psest_2387 0.17 -0.3 2,539,752 + Psest_2387 0.17 -0.3 2,539,752 + Psest_2387 0.17 -0.2 2,539,752 + Psest_2387 0.17 -0.0 2,539,752 + Psest_2387 0.17 +0.2 2,539,752 + Psest_2387 0.17 +0.3 2,539,752 + Psest_2387 0.17 +2.3 2,539,752 + Psest_2387 0.17 +0.1 2,539,752 + Psest_2387 0.17 +0.2 2,539,752 + Psest_2387 0.17 -0.5 2,539,752 + Psest_2387 0.17 +0.5 2,539,752 + Psest_2387 0.17 +1.0 2,539,752 + Psest_2387 0.17 +2.6 2,539,752 + Psest_2387 0.17 -0.4 2,539,752 + Psest_2387 0.17 -0.3 2,539,752 + Psest_2387 0.17 +0.7 2,539,752 + Psest_2387 0.17 -0.3 2,539,752 + Psest_2387 0.17 +1.0 2,539,752 + Psest_2387 0.17 -1.2 2,539,752 + Psest_2387 0.17 +0.6 2,539,753 - Psest_2387 0.17 -0.3 2,539,753 - Psest_2387 0.17 +0.6 2,539,753 - Psest_2387 0.17 +0.1 2,539,753 - Psest_2387 0.17 -2.1 2,539,753 - Psest_2387 0.17 -0.3 2,539,753 - Psest_2387 0.17 +1.2 2,539,753 - Psest_2387 0.17 +0.2 2,539,753 - Psest_2387 0.17 -0.8 2,539,753 - Psest_2387 0.17 +0.0 2,539,753 - Psest_2387 0.17 +0.3 2,539,791 + Psest_2387 0.28 +1.5 2,539,791 + Psest_2387 0.28 -0.0 2,539,791 + Psest_2387 0.28 -0.8 2,539,791 + Psest_2387 0.28 +0.2 2,539,791 + Psest_2387 0.28 +1.4 2,539,791 + Psest_2387 0.28 -0.2 2,539,791 + Psest_2387 0.28 -0.3 2,539,792 - Psest_2387 0.28 -0.5 2,539,792 - Psest_2387 0.28 +1.0 2,539,792 - Psest_2387 0.28 -0.7 2,539,792 - Psest_2387 0.28 -2.9 2,539,792 - Psest_2387 0.28 -0.8 2,539,792 - Psest_2387 0.28 -1.3 2,539,956 + Psest_2387 0.72 -0.8 2,539,980 - Psest_2387 0.78 -1.6 2,540,106 - -0.3 2,540,106 - +0.2 2,540,206 + Psest_2388 0.26 -0.3 2,540,206 + Psest_2388 0.26 -1.1 2,540,206 + Psest_2388 0.26 -1.9 2,540,206 + Psest_2388 0.26 +0.1 2,540,206 + Psest_2388 0.26 -0.4 2,540,206 + Psest_2388 0.26 -1.2 2,540,206 + Psest_2388 0.26 -1.0 2,540,206 + Psest_2388 0.26 -0.5 2,540,206 + Psest_2388 0.26 -0.6 2,540,206 + Psest_2388 0.26 -0.1 2,540,206 + Psest_2388 0.26 +0.0 2,540,206 + Psest_2388 0.26 +0.3 2,540,207 - Psest_2388 0.27 +0.7 2,540,207 - Psest_2388 0.27 +0.4 2,540,207 - Psest_2388 0.27 +0.2 2,540,207 - Psest_2388 0.27 -0.0 2,540,207 - Psest_2388 0.27 +1.0 2,540,207 - Psest_2388 0.27 +1.0 2,540,207 - Psest_2388 0.27 -1.6 2,540,207 - Psest_2388 0.27 -0.6 2,540,275 + Psest_2388 0.40 -0.2 2,540,276 - Psest_2388 0.40 -0.6 2,540,464 + Psest_2388 0.76 +0.2 2,540,465 - Psest_2388 0.76 +0.4 2,540,465 - Psest_2388 0.76 +0.4 2,540,465 - Psest_2388 0.76 -0.3 2,540,493 - Psest_2388 0.81 -0.3 2,540,493 - Psest_2388 0.81 +0.1 2,540,568 + +0.3 2,540,568 + -0.7 2,540,568 + -0.3 2,540,568 + -0.4 2,540,569 - +1.6 2,540,569 - +0.4 2,540,569 - -0.6 2,540,569 - -0.1 2,540,655 + -2.0 2,540,655 + +1.5 2,540,655 + +1.4 2,540,655 + -0.4 2,540,925 + Psest_2389 0.27 -1.2 2,540,925 + Psest_2389 0.27 -0.7 2,540,925 + Psest_2389 0.27 +0.4 2,540,925 + Psest_2389 0.27 -0.6 2,540,925 + Psest_2389 0.27 -0.0 2,540,925 + Psest_2389 0.27 -0.6 2,540,926 - Psest_2389 0.27 -2.8 2,540,926 - Psest_2389 0.27 -0.7 2,540,926 - Psest_2389 0.27 -0.0 2,540,955 + Psest_2389 0.30 +1.0 2,540,956 - Psest_2389 0.30 -2.1 2,540,956 - Psest_2389 0.30 -0.8 2,541,085 - Psest_2389 0.40 -1.0 2,541,258 + Psest_2389 0.54 -0.8 2,541,258 + Psest_2389 0.54 +0.3 2,541,363 + Psest_2389 0.63 +1.0 2,541,363 + Psest_2389 0.63 +0.2 2,541,363 + Psest_2389 0.63 -0.0 2,541,363 + Psest_2389 0.63 -0.3 2,541,363 + Psest_2389 0.63 -1.1 2,541,364 - Psest_2389 0.63 +0.5 2,541,364 - Psest_2389 0.63 -1.2 2,541,364 - Psest_2389 0.63 -0.8 2,541,364 - Psest_2389 0.63 -0.1 2,541,364 - Psest_2389 0.63 -1.5 2,541,364 - Psest_2389 0.63 +1.0 2,541,453 - Psest_2389 0.70 -3.0 2,541,621 + Psest_2389 0.84 -0.3 2,541,622 - Psest_2389 0.84 -0.0 2,541,744 + -1.2 2,541,838 - -1.7 2,541,838 - -0.5 2,541,922 + Psest_2390 0.19 +1.3 2,541,922 + Psest_2390 0.19 -1.2 2,541,926 + Psest_2390 0.20 +1.7 2,541,927 - Psest_2390 0.20 -0.5 2,541,927 - Psest_2390 0.20 +2.0 2,541,927 - Psest_2390 0.20 +0.2 2,541,927 - Psest_2390 0.20 +1.0 2,541,927 - Psest_2390 0.20 -0.9 2,541,927 - Psest_2390 0.20 +0.1 2,541,927 - Psest_2390 0.20 -0.5 2,541,927 - Psest_2390 0.20 +0.2 2,541,927 - Psest_2390 0.20 +0.2 2,542,220 + Psest_2390 0.76 -2.3 2,542,221 - Psest_2390 0.76 -0.5 2,542,247 + Psest_2390 0.81 +0.3 2,542,247 + Psest_2390 0.81 +0.7 2,542,247 + Psest_2390 0.81 -0.2 2,542,248 - Psest_2390 0.81 -0.8 2,542,248 - Psest_2390 0.81 -0.6 2,542,248 - Psest_2390 0.81 +1.3 2,542,248 - Psest_2390 0.81 -0.8 2,542,248 - Psest_2390 0.81 -0.4 2,542,248 - Psest_2390 0.81 -0.1 2,542,398 + -0.6 2,542,527 + Psest_2391 0.21 -0.2 2,542,528 - Psest_2391 0.21 -0.2 2,542,689 + Psest_2391 0.57 -0.5 2,542,689 + Psest_2391 0.57 +2.0 2,542,689 + Psest_2391 0.57 -0.0 2,542,690 - Psest_2391 0.57 -0.2 2,542,764 + Psest_2391 0.74 +0.6 2,542,764 + Psest_2391 0.74 -0.2 2,542,764 + Psest_2391 0.74 +0.4 2,542,765 - Psest_2391 0.74 -1.7 2,542,765 - Psest_2391 0.74 -1.8
Or see this region's nucleotide sequence