Experiment: Nitrite/ lactate media with Nitrite 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3069 and Psest_3070 are separated by 211 nucleotides Psest_3070 and Psest_3071 overlap by 4 nucleotides Psest_3071 and Psest_3072 overlap by 1 nucleotides
Psest_3069: Psest_3069 - 3-oxoadipate enol-lactonase, at 3,245,176 to 3,245,964
_3069
Psest_3070: Psest_3070 - 3-oxoadipyl-CoA thiolase, at 3,246,176 to 3,247,381
_3070
Psest_3071: Psest_3071 - Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit, at 3,247,378 to 3,248,175
_3071
Psest_3072: Psest_3072 - Acyl CoA:acetate/3-ketoacid CoA transferase, alpha subunit, at 3,248,175 to 3,249,032
_3072
Position (kb)
3246
3247
3248 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3245.208 kb on + strand at 3245.208 kb on + strand at 3245.209 kb on - strand at 3245.209 kb on - strand at 3245.237 kb on - strand at 3245.578 kb on + strand, within Psest_3069 at 3245.630 kb on - strand, within Psest_3069 at 3245.630 kb on - strand, within Psest_3069 at 3245.630 kb on - strand, within Psest_3069 at 3245.762 kb on + strand, within Psest_3069 at 3245.953 kb on + strand at 3245.953 kb on + strand at 3245.953 kb on + strand at 3245.984 kb on + strand at 3245.985 kb on - strand at 3246.056 kb on - strand at 3246.561 kb on + strand, within Psest_3070 at 3246.931 kb on + strand, within Psest_3070 at 3246.932 kb on - strand, within Psest_3070 at 3247.006 kb on - strand, within Psest_3070 at 3247.363 kb on + strand at 3247.363 kb on + strand at 3247.364 kb on - strand at 3247.364 kb on - strand at 3247.364 kb on - strand at 3247.367 kb on + strand at 3247.367 kb on + strand at 3247.367 kb on + strand at 3247.368 kb on - strand at 3247.368 kb on - strand at 3247.368 kb on - strand at 3247.368 kb on - strand at 3247.368 kb on - strand at 3247.368 kb on - strand at 3247.368 kb on - strand at 3247.841 kb on + strand, within Psest_3071 at 3247.841 kb on + strand, within Psest_3071 at 3247.842 kb on - strand, within Psest_3071 at 3247.842 kb on - strand, within Psest_3071 at 3247.842 kb on - strand, within Psest_3071 at 3247.842 kb on - strand, within Psest_3071 at 3248.012 kb on + strand, within Psest_3071 at 3248.012 kb on + strand, within Psest_3071 at 3248.013 kb on - strand, within Psest_3071 at 3248.013 kb on - strand, within Psest_3071 at 3248.150 kb on + strand at 3248.150 kb on + strand at 3248.151 kb on - strand at 3248.151 kb on - strand at 3248.151 kb on - strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.246 kb on + strand at 3248.247 kb on - strand at 3248.324 kb on - strand, within Psest_3072 at 3248.327 kb on - strand, within Psest_3072 at 3248.327 kb on - strand, within Psest_3072 at 3248.338 kb on + strand, within Psest_3072 at 3248.339 kb on - strand, within Psest_3072
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrite/ lactate media with Nitrite 20mM remove 3,245,208 + +0.1 3,245,208 + +0.0 3,245,209 - -1.1 3,245,209 - +0.0 3,245,237 - +0.1 3,245,578 + Psest_3069 0.51 -0.8 3,245,630 - Psest_3069 0.58 +0.9 3,245,630 - Psest_3069 0.58 +0.3 3,245,630 - Psest_3069 0.58 +1.6 3,245,762 + Psest_3069 0.74 -1.5 3,245,953 + +0.4 3,245,953 + -1.0 3,245,953 + -0.0 3,245,984 + -1.1 3,245,985 - +0.7 3,246,056 - -1.1 3,246,561 + Psest_3070 0.32 -0.1 3,246,931 + Psest_3070 0.63 -1.2 3,246,932 - Psest_3070 0.63 -0.7 3,247,006 - Psest_3070 0.69 +0.3 3,247,363 + +2.9 3,247,363 + -0.2 3,247,364 - +0.7 3,247,364 - -0.1 3,247,364 - +0.5 3,247,367 + -1.9 3,247,367 + -0.3 3,247,367 + +0.1 3,247,368 - +0.1 3,247,368 - +0.9 3,247,368 - -1.0 3,247,368 - -0.5 3,247,368 - -0.1 3,247,368 - +0.2 3,247,368 - -2.9 3,247,841 + Psest_3071 0.58 +0.9 3,247,841 + Psest_3071 0.58 +0.9 3,247,842 - Psest_3071 0.58 -0.7 3,247,842 - Psest_3071 0.58 +0.8 3,247,842 - Psest_3071 0.58 -0.3 3,247,842 - Psest_3071 0.58 +0.3 3,248,012 + Psest_3071 0.79 -0.7 3,248,012 + Psest_3071 0.79 -1.1 3,248,013 - Psest_3071 0.80 -0.6 3,248,013 - Psest_3071 0.80 -0.2 3,248,150 + -0.5 3,248,150 + -0.1 3,248,151 - -1.2 3,248,151 - +0.0 3,248,151 - +0.1 3,248,242 + +0.3 3,248,242 + +0.2 3,248,242 + +0.6 3,248,242 + -1.2 3,248,242 + -1.1 3,248,242 + +0.3 3,248,242 + +0.2 3,248,242 + +1.5 3,248,242 + -0.3 3,248,242 + +0.2 3,248,242 + +2.9 3,248,242 + +0.3 3,248,242 + +1.2 3,248,242 + -0.4 3,248,242 + +0.3 3,248,242 + -0.2 3,248,242 + -0.1 3,248,242 + -1.1 3,248,242 + -0.0 3,248,242 + -0.1 3,248,242 + +1.3 3,248,243 - -0.4 3,248,243 - -0.2 3,248,243 - +0.6 3,248,243 - +0.1 3,248,243 - +0.1 3,248,243 - +1.5 3,248,243 - -0.1 3,248,243 - -1.7 3,248,243 - +0.3 3,248,243 - -0.7 3,248,243 - -0.9 3,248,243 - -0.4 3,248,243 - -1.2 3,248,243 - +1.9 3,248,243 - +0.7 3,248,243 - -0.3 3,248,243 - +1.3 3,248,243 - -1.3 3,248,243 - -1.1 3,248,243 - -0.4 3,248,243 - +0.4 3,248,243 - +1.1 3,248,246 + -0.4 3,248,247 - +0.5 3,248,324 - Psest_3072 0.17 -0.9 3,248,327 - Psest_3072 0.18 -1.3 3,248,327 - Psest_3072 0.18 -0.4 3,248,338 + Psest_3072 0.19 -1.2 3,248,339 - Psest_3072 0.19 +0.9
Or see this region's nucleotide sequence