Experiment: Nitrite/ lactate media with Nitrite 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1865 and Psest_1866 are separated by 49 nucleotides Psest_1866 and Psest_1867 are separated by 4 nucleotides Psest_1867 and Psest_1868 are separated by 82 nucleotides Psest_1868 and Psest_1869 are separated by 173 nucleotides
Psest_1865: Psest_1865 - Predicted periplasmic protein, at 1,976,150 to 1,976,866
_1865
Psest_1866: Psest_1866 - Uncharacterized protein conserved in bacteria, at 1,976,916 to 1,977,335
_1866
Psest_1867: Psest_1867 - hypothetical protein, at 1,977,340 to 1,977,438
_1867
Psest_1868: Psest_1868 - yecA family protein, at 1,977,521 to 1,978,108
_1868
Psest_1869: Psest_1869 - ATP-dependent DNA helicase RecQ, at 1,978,282 to 1,980,405
_1869
Position (kb)
1977
1978
1979 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1976.544 kb on + strand, within Psest_1865 at 1976.932 kb on - strand at 1976.932 kb on - strand at 1976.932 kb on - strand at 1976.932 kb on - strand at 1976.932 kb on - strand at 1976.932 kb on - strand at 1976.932 kb on - strand at 1976.968 kb on + strand, within Psest_1866 at 1976.968 kb on + strand, within Psest_1866 at 1976.968 kb on + strand, within Psest_1866 at 1976.968 kb on + strand, within Psest_1866 at 1976.969 kb on - strand, within Psest_1866 at 1976.969 kb on - strand, within Psest_1866 at 1976.969 kb on - strand, within Psest_1866 at 1976.969 kb on - strand, within Psest_1866 at 1976.969 kb on - strand, within Psest_1866 at 1976.969 kb on - strand, within Psest_1866 at 1976.969 kb on - strand, within Psest_1866 at 1977.073 kb on + strand, within Psest_1866 at 1977.073 kb on + strand, within Psest_1866 at 1977.073 kb on + strand, within Psest_1866 at 1977.073 kb on + strand, within Psest_1866 at 1977.073 kb on + strand, within Psest_1866 at 1977.074 kb on - strand, within Psest_1866 at 1977.074 kb on - strand, within Psest_1866 at 1977.096 kb on - strand, within Psest_1866 at 1977.176 kb on + strand, within Psest_1866 at 1977.237 kb on + strand, within Psest_1866 at 1977.237 kb on + strand, within Psest_1866 at 1977.579 kb on + strand at 1977.579 kb on + strand at 1977.580 kb on - strand, within Psest_1868 at 1977.884 kb on + strand, within Psest_1868 at 1977.884 kb on + strand, within Psest_1868 at 1977.885 kb on - strand, within Psest_1868 at 1977.885 kb on - strand, within Psest_1868 at 1977.885 kb on - strand, within Psest_1868 at 1977.933 kb on + strand, within Psest_1868 at 1977.933 kb on + strand, within Psest_1868 at 1977.933 kb on + strand, within Psest_1868 at 1977.933 kb on + strand, within Psest_1868 at 1978.032 kb on + strand, within Psest_1868 at 1978.033 kb on - strand, within Psest_1868 at 1978.033 kb on - strand, within Psest_1868 at 1978.116 kb on + strand at 1978.116 kb on + strand at 1978.183 kb on + strand at 1978.252 kb on + strand at 1978.252 kb on + strand at 1978.252 kb on + strand at 1978.252 kb on + strand at 1978.253 kb on - strand at 1978.253 kb on - strand at 1978.254 kb on + strand at 1978.254 kb on + strand at 1978.254 kb on + strand at 1978.254 kb on + strand at 1978.255 kb on - strand at 1978.255 kb on - strand at 1978.662 kb on - strand, within Psest_1869 at 1978.741 kb on + strand, within Psest_1869 at 1978.742 kb on - strand, within Psest_1869 at 1978.900 kb on + strand, within Psest_1869
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrite/ lactate media with Nitrite 20mM remove 1,976,544 + Psest_1865 0.55 -0.1 1,976,932 - +0.6 1,976,932 - +0.7 1,976,932 - +0.4 1,976,932 - -0.4 1,976,932 - +0.0 1,976,932 - +0.4 1,976,932 - -1.1 1,976,968 + Psest_1866 0.12 -0.1 1,976,968 + Psest_1866 0.12 -0.8 1,976,968 + Psest_1866 0.12 +0.0 1,976,968 + Psest_1866 0.12 -0.8 1,976,969 - Psest_1866 0.13 -0.4 1,976,969 - Psest_1866 0.13 -0.2 1,976,969 - Psest_1866 0.13 -0.9 1,976,969 - Psest_1866 0.13 +0.9 1,976,969 - Psest_1866 0.13 +0.7 1,976,969 - Psest_1866 0.13 +1.3 1,976,969 - Psest_1866 0.13 +0.2 1,977,073 + Psest_1866 0.37 -1.3 1,977,073 + Psest_1866 0.37 +0.7 1,977,073 + Psest_1866 0.37 -0.4 1,977,073 + Psest_1866 0.37 +0.9 1,977,073 + Psest_1866 0.37 -0.9 1,977,074 - Psest_1866 0.38 +0.5 1,977,074 - Psest_1866 0.38 -0.9 1,977,096 - Psest_1866 0.43 +0.4 1,977,176 + Psest_1866 0.62 +0.3 1,977,237 + Psest_1866 0.76 +0.1 1,977,237 + Psest_1866 0.76 +0.3 1,977,579 + -1.3 1,977,579 + +0.6 1,977,580 - Psest_1868 0.10 -0.7 1,977,884 + Psest_1868 0.62 -0.5 1,977,884 + Psest_1868 0.62 +0.9 1,977,885 - Psest_1868 0.62 -1.3 1,977,885 - Psest_1868 0.62 -0.5 1,977,885 - Psest_1868 0.62 -0.4 1,977,933 + Psest_1868 0.70 +0.5 1,977,933 + Psest_1868 0.70 -0.7 1,977,933 + Psest_1868 0.70 +1.9 1,977,933 + Psest_1868 0.70 +0.3 1,978,032 + Psest_1868 0.87 -0.7 1,978,033 - Psest_1868 0.87 -2.0 1,978,033 - Psest_1868 0.87 +0.3 1,978,116 + -0.7 1,978,116 + +0.3 1,978,183 + +0.3 1,978,252 + -0.4 1,978,252 + +0.9 1,978,252 + +0.6 1,978,252 + +0.9 1,978,253 - -0.1 1,978,253 - +2.9 1,978,254 + +1.2 1,978,254 + -0.6 1,978,254 + +0.9 1,978,254 + +0.2 1,978,255 - -1.1 1,978,255 - -1.2 1,978,662 - Psest_1869 0.18 +0.9 1,978,741 + Psest_1869 0.22 +1.3 1,978,742 - Psest_1869 0.22 +0.5 1,978,900 + Psest_1869 0.29 -0.3
Or see this region's nucleotide sequence