Experiment: Nitrite/ lactate media with Nitrite 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0987 and Psest_0988 are separated by 69 nucleotides Psest_0988 and Psest_0989 are separated by 17 nucleotides Psest_0989 and Psest_0990 overlap by 1 nucleotides Psest_0990 and Psest_0991 are separated by 85 nucleotides
Psest_0987: Psest_0987 - Predicted periplasmic or secreted lipoprotein, at 1,069,527 to 1,069,895
_0987
Psest_0988: Psest_0988 - Protein of unknown function (DUF2845)., at 1,069,965 to 1,070,273
_0988
Psest_0989: Psest_0989 - Protein of unknown function (DUF2845)., at 1,070,291 to 1,070,587
_0989
Psest_0990: Psest_0990 - Outer membrane protein and related peptidoglycan-associated (lipo)proteins, at 1,070,587 to 1,073,478
_0990
Psest_0991: Psest_0991 - Predicted SAM-dependent methyltransferases, at 1,073,564 to 1,074,580
_0991
Position (kb)
1070
1071
1072
1073
1074 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1069.759 kb on + strand, within Psest_0987 at 1069.759 kb on + strand, within Psest_0987 at 1069.760 kb on - strand, within Psest_0987 at 1069.760 kb on - strand, within Psest_0987 at 1069.915 kb on - strand at 1069.915 kb on - strand at 1069.966 kb on + strand at 1069.966 kb on + strand at 1070.048 kb on - strand, within Psest_0988 at 1070.086 kb on + strand, within Psest_0988 at 1070.107 kb on + strand, within Psest_0988 at 1070.205 kb on - strand, within Psest_0988 at 1070.424 kb on + strand, within Psest_0989 at 1070.425 kb on - strand, within Psest_0989 at 1070.571 kb on + strand at 1070.572 kb on - strand at 1070.572 kb on - strand at 1070.617 kb on + strand at 1070.617 kb on + strand at 1070.617 kb on + strand at 1070.617 kb on + strand at 1070.617 kb on + strand at 1070.618 kb on - strand at 1070.618 kb on - strand at 1070.618 kb on - strand at 1070.659 kb on + strand at 1070.659 kb on + strand at 1070.659 kb on + strand at 1070.659 kb on + strand at 1070.660 kb on - strand at 1070.660 kb on - strand at 1070.660 kb on - strand at 1070.663 kb on + strand at 1070.663 kb on + strand at 1070.664 kb on - strand at 1070.664 kb on - strand at 1070.664 kb on - strand at 1070.700 kb on - strand at 1070.743 kb on + strand at 1070.859 kb on - strand at 1070.859 kb on - strand at 1070.902 kb on + strand, within Psest_0990 at 1070.903 kb on - strand, within Psest_0990 at 1070.903 kb on - strand, within Psest_0990 at 1071.347 kb on - strand, within Psest_0990 at 1071.630 kb on + strand, within Psest_0990 at 1072.014 kb on + strand, within Psest_0990 at 1072.015 kb on - strand, within Psest_0990 at 1072.075 kb on + strand, within Psest_0990 at 1072.075 kb on + strand, within Psest_0990 at 1072.076 kb on - strand, within Psest_0990 at 1072.159 kb on + strand, within Psest_0990 at 1072.159 kb on + strand, within Psest_0990 at 1072.159 kb on + strand, within Psest_0990 at 1072.159 kb on + strand, within Psest_0990 at 1072.159 kb on + strand, within Psest_0990 at 1072.159 kb on + strand, within Psest_0990 at 1072.159 kb on + strand, within Psest_0990 at 1072.402 kb on + strand, within Psest_0990 at 1072.402 kb on + strand, within Psest_0990 at 1072.790 kb on + strand, within Psest_0990 at 1072.791 kb on - strand, within Psest_0990 at 1072.791 kb on - strand, within Psest_0990 at 1072.951 kb on - strand, within Psest_0990 at 1072.951 kb on - strand, within Psest_0990 at 1072.977 kb on - strand, within Psest_0990 at 1072.989 kb on + strand, within Psest_0990 at 1072.989 kb on + strand, within Psest_0990 at 1072.990 kb on - strand, within Psest_0990 at 1072.990 kb on - strand, within Psest_0990 at 1072.990 kb on - strand, within Psest_0990 at 1072.990 kb on - strand, within Psest_0990 at 1073.162 kb on + strand, within Psest_0990 at 1073.162 kb on + strand, within Psest_0990 at 1073.162 kb on + strand, within Psest_0990 at 1073.162 kb on + strand, within Psest_0990 at 1073.162 kb on + strand, within Psest_0990 at 1073.163 kb on - strand, within Psest_0990 at 1073.163 kb on - strand, within Psest_0990 at 1073.163 kb on - strand, within Psest_0990 at 1073.163 kb on - strand, within Psest_0990 at 1073.163 kb on - strand, within Psest_0990 at 1073.163 kb on - strand, within Psest_0990 at 1073.163 kb on - strand, within Psest_0990 at 1073.235 kb on - strand at 1073.325 kb on + strand at 1073.326 kb on - strand at 1073.354 kb on + strand at 1073.355 kb on - strand at 1073.488 kb on + strand at 1073.489 kb on - strand at 1073.515 kb on + strand at 1073.516 kb on - strand at 1073.791 kb on - strand, within Psest_0991 at 1073.852 kb on + strand, within Psest_0991 at 1073.853 kb on - strand, within Psest_0991 at 1073.956 kb on - strand, within Psest_0991 at 1073.956 kb on - strand, within Psest_0991 at 1074.101 kb on - strand, within Psest_0991 at 1074.101 kb on - strand, within Psest_0991 at 1074.102 kb on + strand, within Psest_0991 at 1074.102 kb on + strand, within Psest_0991 at 1074.102 kb on + strand, within Psest_0991 at 1074.127 kb on + strand, within Psest_0991 at 1074.212 kb on + strand, within Psest_0991 at 1074.327 kb on + strand, within Psest_0991
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrite/ lactate media with Nitrite 20mM remove 1,069,759 + Psest_0987 0.63 -0.4 1,069,759 + Psest_0987 0.63 -2.0 1,069,760 - Psest_0987 0.63 -0.4 1,069,760 - Psest_0987 0.63 -0.1 1,069,915 - +0.0 1,069,915 - -0.1 1,069,966 + +0.6 1,069,966 + +0.2 1,070,048 - Psest_0988 0.27 +1.9 1,070,086 + Psest_0988 0.39 -0.7 1,070,107 + Psest_0988 0.46 +0.2 1,070,205 - Psest_0988 0.78 -0.8 1,070,424 + Psest_0989 0.45 +0.6 1,070,425 - Psest_0989 0.45 +1.3 1,070,571 + -1.4 1,070,572 - -0.2 1,070,572 - -0.4 1,070,617 + -0.7 1,070,617 + -0.4 1,070,617 + +0.4 1,070,617 + +0.4 1,070,617 + +1.3 1,070,618 - -1.3 1,070,618 - -0.9 1,070,618 - -0.1 1,070,659 + -0.1 1,070,659 + +1.9 1,070,659 + +1.9 1,070,659 + +0.5 1,070,660 - -0.8 1,070,660 - -1.4 1,070,660 - -0.1 1,070,663 + -0.7 1,070,663 + -2.4 1,070,664 - -0.4 1,070,664 - +0.9 1,070,664 - -0.9 1,070,700 - +1.5 1,070,743 + +0.3 1,070,859 - -0.7 1,070,859 - +0.3 1,070,902 + Psest_0990 0.11 -1.3 1,070,903 - Psest_0990 0.11 +0.3 1,070,903 - Psest_0990 0.11 -0.8 1,071,347 - Psest_0990 0.26 +0.5 1,071,630 + Psest_0990 0.36 +1.9 1,072,014 + Psest_0990 0.49 +0.7 1,072,015 - Psest_0990 0.49 -0.4 1,072,075 + Psest_0990 0.51 -0.4 1,072,075 + Psest_0990 0.51 -0.5 1,072,076 - Psest_0990 0.51 +0.4 1,072,159 + Psest_0990 0.54 +0.9 1,072,159 + Psest_0990 0.54 -1.6 1,072,159 + Psest_0990 0.54 +1.6 1,072,159 + Psest_0990 0.54 -0.1 1,072,159 + Psest_0990 0.54 -0.5 1,072,159 + Psest_0990 0.54 -1.1 1,072,159 + Psest_0990 0.54 -0.5 1,072,402 + Psest_0990 0.63 -0.8 1,072,402 + Psest_0990 0.63 +2.9 1,072,790 + Psest_0990 0.76 -1.4 1,072,791 - Psest_0990 0.76 +0.1 1,072,791 - Psest_0990 0.76 -0.7 1,072,951 - Psest_0990 0.82 -0.4 1,072,951 - Psest_0990 0.82 -0.7 1,072,977 - Psest_0990 0.83 +0.5 1,072,989 + Psest_0990 0.83 +0.7 1,072,989 + Psest_0990 0.83 -0.8 1,072,990 - Psest_0990 0.83 +0.9 1,072,990 - Psest_0990 0.83 -0.1 1,072,990 - Psest_0990 0.83 -0.9 1,072,990 - Psest_0990 0.83 +1.1 1,073,162 + Psest_0990 0.89 -2.0 1,073,162 + Psest_0990 0.89 +0.6 1,073,162 + Psest_0990 0.89 -0.7 1,073,162 + Psest_0990 0.89 +0.4 1,073,162 + Psest_0990 0.89 -0.4 1,073,163 - Psest_0990 0.89 -0.4 1,073,163 - Psest_0990 0.89 -0.1 1,073,163 - Psest_0990 0.89 +0.3 1,073,163 - Psest_0990 0.89 -1.9 1,073,163 - Psest_0990 0.89 -1.6 1,073,163 - Psest_0990 0.89 -0.5 1,073,163 - Psest_0990 0.89 -1.1 1,073,235 - -0.4 1,073,325 + -0.3 1,073,326 - -1.0 1,073,354 + +0.8 1,073,355 - +0.1 1,073,488 + -0.8 1,073,489 - -0.5 1,073,515 + +0.5 1,073,516 - +0.6 1,073,791 - Psest_0991 0.22 -0.3 1,073,852 + Psest_0991 0.28 +1.4 1,073,853 - Psest_0991 0.28 -0.1 1,073,956 - Psest_0991 0.39 -1.7 1,073,956 - Psest_0991 0.39 +1.9 1,074,101 - Psest_0991 0.53 +1.9 1,074,101 - Psest_0991 0.53 +1.8 1,074,102 + Psest_0991 0.53 +0.4 1,074,102 + Psest_0991 0.53 +0.5 1,074,102 + Psest_0991 0.53 -0.4 1,074,127 + Psest_0991 0.55 -0.9 1,074,212 + Psest_0991 0.64 -0.4 1,074,327 + Psest_0991 0.75 +0.2
Or see this region's nucleotide sequence