Experiment: Nitrite/ lactate media with Nitrite 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0815 and Psest_0816 are separated by 69 nucleotides Psest_0816 and Psest_0817 are separated by 49 nucleotides Psest_0817 and Psest_0818 are separated by 24 nucleotides Psest_0818 and Psest_0819 are separated by 146 nucleotides Psest_0819 and Psest_0820 are separated by 18 nucleotides Psest_0820 and Psest_0821 overlap by 4 nucleotides
Psest_0815: Psest_0815 - Cytochrome c, mono- and diheme variants, at 889,993 to 891,675
_0815
Psest_0816: Psest_0816 - periplasmic nitrate (or nitrite) reductase c-type cytochrome, NapC/NirT family, at 891,745 to 892,350
_0816
Psest_0817: Psest_0817 - Ethylbenzene dehydrogenase., at 892,400 to 893,275
_0817
Psest_0818: Psest_0818 - hypothetical protein, at 893,300 to 893,446
_0818
Psest_0819: Psest_0819 - Cytochrome c551/c552, at 893,593 to 893,907
_0819
Psest_0820: Psest_0820 - hypothetical protein, at 893,926 to 894,267
_0820
Psest_0821: Psest_0821 - Cytochrome D1 heme domain., at 894,264 to 895,439
_0821
Position (kb) 892
893
894 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 891.571 kb on + strand at 891.572 kb on - strand at 891.661 kb on + strand at 891.661 kb on + strand at 891.662 kb on - strand at 891.662 kb on - strand at 891.662 kb on - strand at 891.662 kb on - strand at 891.662 kb on - strand at 891.662 kb on - strand at 891.671 kb on - strand at 891.673 kb on + strand at 891.674 kb on - strand at 891.697 kb on - strand at 891.959 kb on - strand, within Psest_0816 at 891.959 kb on - strand, within Psest_0816 at 892.326 kb on + strand at 892.327 kb on - strand at 892.327 kb on - strand at 892.327 kb on - strand at 892.327 kb on - strand at 892.353 kb on + strand at 892.354 kb on - strand at 892.462 kb on + strand at 892.743 kb on - strand, within Psest_0817 at 892.743 kb on - strand, within Psest_0817 at 892.820 kb on + strand, within Psest_0817 at 892.820 kb on + strand, within Psest_0817 at 892.820 kb on + strand, within Psest_0817 at 892.820 kb on + strand, within Psest_0817 at 892.820 kb on + strand, within Psest_0817 at 892.820 kb on + strand, within Psest_0817 at 892.821 kb on - strand, within Psest_0817 at 892.821 kb on - strand, within Psest_0817 at 892.821 kb on - strand, within Psest_0817 at 892.931 kb on + strand, within Psest_0817 at 892.931 kb on + strand, within Psest_0817 at 892.931 kb on + strand, within Psest_0817 at 892.931 kb on + strand, within Psest_0817 at 892.931 kb on + strand, within Psest_0817 at 892.932 kb on - strand, within Psest_0817 at 892.932 kb on - strand, within Psest_0817 at 892.932 kb on - strand, within Psest_0817 at 892.932 kb on - strand, within Psest_0817 at 892.964 kb on + strand, within Psest_0817 at 892.964 kb on + strand, within Psest_0817 at 892.965 kb on - strand, within Psest_0817 at 892.965 kb on - strand, within Psest_0817 at 892.967 kb on + strand, within Psest_0817 at 892.967 kb on + strand, within Psest_0817 at 892.967 kb on + strand, within Psest_0817 at 892.968 kb on - strand, within Psest_0817 at 892.968 kb on - strand, within Psest_0817 at 893.086 kb on + strand, within Psest_0817 at 893.086 kb on + strand, within Psest_0817 at 893.087 kb on - strand, within Psest_0817 at 893.087 kb on - strand, within Psest_0817 at 893.159 kb on + strand, within Psest_0817 at 893.159 kb on + strand, within Psest_0817 at 893.159 kb on + strand, within Psest_0817 at 893.392 kb on - strand, within Psest_0818 at 893.585 kb on + strand at 893.585 kb on + strand at 893.585 kb on + strand at 893.585 kb on + strand at 893.585 kb on + strand at 893.586 kb on - strand at 893.586 kb on - strand at 893.591 kb on + strand at 893.591 kb on + strand at 893.591 kb on + strand at 893.591 kb on + strand at 893.591 kb on + strand at 893.592 kb on - strand at 893.592 kb on - strand at 893.592 kb on - strand at 893.905 kb on + strand at 893.905 kb on + strand at 893.905 kb on + strand at 893.905 kb on + strand at 893.905 kb on + strand at 893.905 kb on + strand at 893.906 kb on - strand at 893.906 kb on - strand at 893.906 kb on - strand at 893.906 kb on - strand at 893.906 kb on - strand at 893.906 kb on - strand at 893.906 kb on - strand at 894.047 kb on + strand, within Psest_0820 at 894.047 kb on + strand, within Psest_0820 at 894.047 kb on + strand, within Psest_0820 at 894.048 kb on - strand, within Psest_0820 at 894.048 kb on - strand, within Psest_0820 at 894.048 kb on - strand, within Psest_0820 at 894.048 kb on - strand, within Psest_0820 at 894.048 kb on - strand, within Psest_0820
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrite/ lactate media with Nitrite 20mM remove 891,571 + +1.9 891,572 - -3.1 891,661 + -0.9 891,661 + -1.0 891,662 - -0.6 891,662 - -0.4 891,662 - -0.2 891,662 - -1.3 891,662 - +1.9 891,662 - +0.4 891,671 - -2.7 891,673 + -0.5 891,674 - -2.1 891,697 - -2.1 891,959 - Psest_0816 0.35 +0.4 891,959 - Psest_0816 0.35 +0.9 892,326 + -2.9 892,327 - -1.5 892,327 - -1.4 892,327 - +1.4 892,327 - -1.3 892,353 + -0.6 892,354 - -0.1 892,462 + -3.3 892,743 - Psest_0817 0.39 -0.5 892,743 - Psest_0817 0.39 -0.2 892,820 + Psest_0817 0.48 +0.4 892,820 + Psest_0817 0.48 -0.1 892,820 + Psest_0817 0.48 +1.4 892,820 + Psest_0817 0.48 -0.4 892,820 + Psest_0817 0.48 -1.1 892,820 + Psest_0817 0.48 +1.4 892,821 - Psest_0817 0.48 -1.5 892,821 - Psest_0817 0.48 -1.0 892,821 - Psest_0817 0.48 -0.9 892,931 + Psest_0817 0.61 -0.4 892,931 + Psest_0817 0.61 -2.1 892,931 + Psest_0817 0.61 -1.5 892,931 + Psest_0817 0.61 -2.1 892,931 + Psest_0817 0.61 +0.4 892,932 - Psest_0817 0.61 -0.2 892,932 - Psest_0817 0.61 -0.4 892,932 - Psest_0817 0.61 -1.6 892,932 - Psest_0817 0.61 -0.5 892,964 + Psest_0817 0.64 -1.2 892,964 + Psest_0817 0.64 -0.1 892,965 - Psest_0817 0.64 -1.1 892,965 - Psest_0817 0.64 -3.1 892,967 + Psest_0817 0.65 +0.9 892,967 + Psest_0817 0.65 -1.0 892,967 + Psest_0817 0.65 -1.4 892,968 - Psest_0817 0.65 -2.0 892,968 - Psest_0817 0.65 -2.4 893,086 + Psest_0817 0.78 -0.9 893,086 + Psest_0817 0.78 -0.4 893,087 - Psest_0817 0.78 -0.9 893,087 - Psest_0817 0.78 -2.3 893,159 + Psest_0817 0.87 +0.6 893,159 + Psest_0817 0.87 -2.6 893,159 + Psest_0817 0.87 +0.4 893,392 - Psest_0818 0.63 +0.9 893,585 + -2.5 893,585 + +1.9 893,585 + -0.9 893,585 + +0.9 893,585 + -1.9 893,586 - -3.1 893,586 - -3.1 893,591 + -2.6 893,591 + -1.4 893,591 + -0.9 893,591 + -2.3 893,591 + -2.9 893,592 - -1.5 893,592 - -0.9 893,592 - -1.2 893,905 + -1.3 893,905 + -1.4 893,905 + -0.1 893,905 + -1.6 893,905 + -1.1 893,905 + +0.4 893,906 - -0.2 893,906 - +0.4 893,906 - -3.2 893,906 - -1.4 893,906 - -0.9 893,906 - -2.8 893,906 - -2.3 894,047 + Psest_0820 0.35 -1.1 894,047 + Psest_0820 0.35 -1.4 894,047 + Psest_0820 0.35 +0.9 894,048 - Psest_0820 0.36 -2.3 894,048 - Psest_0820 0.36 -1.2 894,048 - Psest_0820 0.36 -0.1 894,048 - Psest_0820 0.36 -1.6 894,048 - Psest_0820 0.36 -3.0
Or see this region's nucleotide sequence