Experiment: Nitrate/ lactate media with Nitrate 250mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0307 and Psest_0308 are separated by 25 nucleotides Psest_0308 and Psest_0309 are separated by 262 nucleotides Psest_0309 and Psest_0310 are separated by 14 nucleotides
Psest_0307: Psest_0307 - Cytochrome B561, at 326,922 to 327,476
_0307
Psest_0308: Psest_0308 - Uncharacterized conserved protein, at 327,502 to 328,077
_0308
Psest_0309: Psest_0309 - RND family efflux transporter, MFP subunit, at 328,340 to 329,482
_0309
Psest_0310: Psest_0310 - The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family, at 329,497 to 332,634
_0310
Position (kb) 328
329
330 Strain fitness (log2 ratio) -1
0
1
2
3 at 327.360 kb on + strand, within Psest_0307 at 327.361 kb on - strand, within Psest_0307 at 327.394 kb on + strand, within Psest_0307 at 327.394 kb on + strand, within Psest_0307 at 327.394 kb on + strand, within Psest_0307 at 327.394 kb on + strand, within Psest_0307 at 327.394 kb on + strand, within Psest_0307 at 327.394 kb on + strand, within Psest_0307 at 327.394 kb on + strand, within Psest_0307 at 327.394 kb on + strand, within Psest_0307 at 327.395 kb on - strand, within Psest_0307 at 327.395 kb on - strand, within Psest_0307 at 327.395 kb on - strand, within Psest_0307 at 327.395 kb on - strand, within Psest_0307 at 327.395 kb on - strand, within Psest_0307 at 327.395 kb on - strand, within Psest_0307 at 327.395 kb on - strand, within Psest_0307 at 327.634 kb on + strand, within Psest_0308 at 327.634 kb on + strand, within Psest_0308 at 328.071 kb on + strand at 328.071 kb on + strand at 328.072 kb on - strand at 328.075 kb on + strand at 328.075 kb on + strand at 328.075 kb on + strand at 328.075 kb on + strand at 328.075 kb on + strand at 328.075 kb on + strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.076 kb on - strand at 328.087 kb on - strand at 328.171 kb on + strand at 328.171 kb on + strand at 328.171 kb on + strand at 328.172 kb on - strand at 328.172 kb on - strand at 328.207 kb on + strand at 328.207 kb on + strand at 328.207 kb on + strand at 328.207 kb on + strand at 328.207 kb on + strand at 328.207 kb on + strand at 328.207 kb on + strand at 328.208 kb on - strand at 328.208 kb on - strand at 328.254 kb on + strand at 328.254 kb on + strand at 328.254 kb on + strand at 328.254 kb on + strand at 328.254 kb on + strand at 328.254 kb on + strand at 328.254 kb on + strand at 328.254 kb on + strand at 328.255 kb on - strand at 328.258 kb on + strand at 328.258 kb on + strand at 328.260 kb on + strand at 328.260 kb on + strand at 328.260 kb on + strand at 328.261 kb on - strand at 328.736 kb on + strand, within Psest_0309 at 328.926 kb on - strand, within Psest_0309 at 329.162 kb on - strand, within Psest_0309 at 329.491 kb on + strand at 329.491 kb on + strand at 329.491 kb on + strand at 329.965 kb on + strand, within Psest_0310 at 330.104 kb on + strand, within Psest_0310 at 330.105 kb on - strand, within Psest_0310 at 330.105 kb on - strand, within Psest_0310 at 330.105 kb on - strand, within Psest_0310 at 330.105 kb on - strand, within Psest_0310 at 330.105 kb on - strand, within Psest_0310 at 330.413 kb on + strand, within Psest_0310 at 330.414 kb on - strand, within Psest_0310 at 330.475 kb on + strand, within Psest_0310
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate/ lactate media with Nitrate 250mM remove 327,360 + Psest_0307 0.79 +0.2 327,361 - Psest_0307 0.79 -0.2 327,394 + Psest_0307 0.85 +0.9 327,394 + Psest_0307 0.85 +0.0 327,394 + Psest_0307 0.85 +1.0 327,394 + Psest_0307 0.85 +1.7 327,394 + Psest_0307 0.85 -0.8 327,394 + Psest_0307 0.85 -0.3 327,394 + Psest_0307 0.85 -0.2 327,394 + Psest_0307 0.85 +1.0 327,395 - Psest_0307 0.85 +2.0 327,395 - Psest_0307 0.85 -1.4 327,395 - Psest_0307 0.85 -0.8 327,395 - Psest_0307 0.85 -0.4 327,395 - Psest_0307 0.85 +0.6 327,395 - Psest_0307 0.85 -0.2 327,395 - Psest_0307 0.85 -0.4 327,634 + Psest_0308 0.23 +2.0 327,634 + Psest_0308 0.23 +1.0 328,071 + +1.0 328,071 + -0.4 328,072 - -0.3 328,075 + +1.6 328,075 + +0.2 328,075 + +0.0 328,075 + +0.9 328,075 + +1.0 328,075 + -0.6 328,076 - +0.0 328,076 - +0.3 328,076 - +1.0 328,076 - +1.0 328,076 - -0.4 328,076 - +1.0 328,076 - -0.2 328,076 - +0.2 328,076 - -0.8 328,076 - +0.9 328,076 - +0.9 328,076 - -0.5 328,087 - -0.8 328,171 + +0.4 328,171 + +1.3 328,171 + +0.5 328,172 - +0.0 328,172 - -1.3 328,207 + -0.4 328,207 + +0.4 328,207 + +0.5 328,207 + -0.2 328,207 + -0.5 328,207 + +0.2 328,207 + +0.6 328,208 - -0.9 328,208 - -1.0 328,254 + +3.0 328,254 + +0.2 328,254 + -0.8 328,254 + -0.4 328,254 + +1.3 328,254 + +0.3 328,254 + +0.4 328,254 + -0.4 328,255 - -0.8 328,258 + -0.3 328,258 + -0.1 328,260 + +1.0 328,260 + -0.4 328,260 + +0.4 328,261 - +1.0 328,736 + Psest_0309 0.35 +2.0 328,926 - Psest_0309 0.51 +0.0 329,162 - Psest_0309 0.72 +0.4 329,491 + +1.0 329,491 + +1.0 329,491 + +1.0 329,965 + Psest_0310 0.15 -0.3 330,104 + Psest_0310 0.19 +0.0 330,105 - Psest_0310 0.19 +1.0 330,105 - Psest_0310 0.19 +1.4 330,105 - Psest_0310 0.19 +0.4 330,105 - Psest_0310 0.19 +1.0 330,105 - Psest_0310 0.19 +1.0 330,413 + Psest_0310 0.29 +0.2 330,414 - Psest_0310 0.29 +0.4 330,475 + Psest_0310 0.31 -0.3
Or see this region's nucleotide sequence