Experiment: Nitrate/ lactate media with Nitrate 250mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2512 and Psest_2513 are separated by 120 nucleotides Psest_2513 and Psest_2514 are separated by 102 nucleotides Psest_2514 and Psest_2515 are separated by 147 nucleotides
Psest_2512: Psest_2512 - Kef-type K+ transport systems, predicted NAD-binding component, at 2,690,670 to 2,691,521
_2512
Psest_2513: Psest_2513 - Predicted membrane protein, at 2,691,642 to 2,692,055
_2513
Psest_2514: Psest_2514 - Predicted membrane protein, at 2,692,158 to 2,692,637
_2514
Psest_2515: Psest_2515 - cytochrome c oxidase, subunit II, at 2,692,785 to 2,693,675
_2515
Position (kb)
2691
2692
2693 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2690.714 kb on - strand at 2690.715 kb on + strand at 2690.715 kb on + strand at 2690.715 kb on + strand at 2690.715 kb on + strand at 2690.715 kb on + strand at 2690.715 kb on + strand at 2690.715 kb on + strand at 2690.716 kb on - strand at 2690.716 kb on - strand at 2690.716 kb on - strand at 2690.716 kb on - strand at 2690.716 kb on - strand at 2690.716 kb on - strand at 2690.716 kb on - strand at 2690.716 kb on - strand at 2690.716 kb on - strand at 2690.716 kb on - strand at 2690.716 kb on - strand at 2690.716 kb on - strand at 2690.895 kb on + strand, within Psest_2512 at 2690.896 kb on - strand, within Psest_2512 at 2690.910 kb on + strand, within Psest_2512 at 2690.910 kb on + strand, within Psest_2512 at 2690.910 kb on + strand, within Psest_2512 at 2690.910 kb on + strand, within Psest_2512 at 2690.911 kb on - strand, within Psest_2512 at 2690.911 kb on - strand, within Psest_2512 at 2690.911 kb on - strand, within Psest_2512 at 2690.934 kb on + strand, within Psest_2512 at 2690.935 kb on - strand, within Psest_2512 at 2690.935 kb on - strand, within Psest_2512 at 2690.935 kb on - strand, within Psest_2512 at 2690.935 kb on - strand, within Psest_2512 at 2690.935 kb on - strand, within Psest_2512 at 2690.949 kb on + strand, within Psest_2512 at 2690.950 kb on - strand, within Psest_2512 at 2691.015 kb on + strand, within Psest_2512 at 2691.015 kb on + strand, within Psest_2512 at 2691.047 kb on - strand, within Psest_2512 at 2691.052 kb on + strand, within Psest_2512 at 2691.062 kb on - strand, within Psest_2512 at 2691.175 kb on - strand, within Psest_2512 at 2691.175 kb on - strand, within Psest_2512 at 2691.189 kb on + strand, within Psest_2512 at 2691.190 kb on - strand, within Psest_2512 at 2691.190 kb on - strand, within Psest_2512 at 2691.190 kb on - strand, within Psest_2512 at 2691.190 kb on - strand, within Psest_2512 at 2691.240 kb on + strand, within Psest_2512 at 2691.240 kb on + strand, within Psest_2512 at 2691.241 kb on - strand, within Psest_2512 at 2691.241 kb on - strand, within Psest_2512 at 2691.241 kb on - strand, within Psest_2512 at 2691.241 kb on - strand, within Psest_2512 at 2691.264 kb on - strand, within Psest_2512 at 2691.516 kb on - strand at 2691.524 kb on + strand at 2691.524 kb on + strand at 2691.525 kb on - strand at 2691.703 kb on - strand, within Psest_2513 at 2691.703 kb on - strand, within Psest_2513 at 2691.975 kb on + strand, within Psest_2513 at 2691.976 kb on - strand, within Psest_2513 at 2691.976 kb on - strand, within Psest_2513 at 2691.976 kb on - strand, within Psest_2513 at 2692.517 kb on - strand, within Psest_2514 at 2692.607 kb on + strand at 2692.607 kb on + strand at 2692.657 kb on + strand at 2692.657 kb on + strand at 2692.657 kb on + strand at 2692.657 kb on + strand at 2692.658 kb on - strand at 2692.840 kb on + strand at 2692.869 kb on + strand at 2692.869 kb on + strand at 2692.869 kb on + strand at 2692.870 kb on - strand at 2692.896 kb on + strand, within Psest_2515 at 2692.896 kb on + strand, within Psest_2515 at 2692.896 kb on + strand, within Psest_2515 at 2692.896 kb on + strand, within Psest_2515 at 2692.896 kb on + strand, within Psest_2515 at 2692.896 kb on + strand, within Psest_2515 at 2692.896 kb on + strand, within Psest_2515 at 2692.896 kb on + strand, within Psest_2515 at 2692.896 kb on + strand, within Psest_2515 at 2692.897 kb on - strand, within Psest_2515 at 2692.897 kb on - strand, within Psest_2515 at 2692.897 kb on - strand, within Psest_2515 at 2692.897 kb on - strand, within Psest_2515 at 2692.897 kb on - strand, within Psest_2515 at 2692.897 kb on - strand, within Psest_2515 at 2693.047 kb on + strand, within Psest_2515
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate/ lactate media with Nitrate 250mM remove 2,690,714 - +1.0 2,690,715 + -0.0 2,690,715 + -0.9 2,690,715 + -0.4 2,690,715 + +0.2 2,690,715 + +1.3 2,690,715 + -0.4 2,690,715 + -0.4 2,690,716 - -0.0 2,690,716 - +0.2 2,690,716 - -0.4 2,690,716 - +0.5 2,690,716 - -0.4 2,690,716 - -0.9 2,690,716 - +0.5 2,690,716 - -1.9 2,690,716 - +0.0 2,690,716 - -0.6 2,690,716 - +1.6 2,690,716 - +0.9 2,690,895 + Psest_2512 0.26 -0.1 2,690,896 - Psest_2512 0.27 +0.9 2,690,910 + Psest_2512 0.28 -0.1 2,690,910 + Psest_2512 0.28 +0.2 2,690,910 + Psest_2512 0.28 +1.2 2,690,910 + Psest_2512 0.28 -0.1 2,690,911 - Psest_2512 0.28 -2.0 2,690,911 - Psest_2512 0.28 -0.1 2,690,911 - Psest_2512 0.28 -0.6 2,690,934 + Psest_2512 0.31 +1.3 2,690,935 - Psest_2512 0.31 +0.9 2,690,935 - Psest_2512 0.31 -0.1 2,690,935 - Psest_2512 0.31 +0.3 2,690,935 - Psest_2512 0.31 -0.6 2,690,935 - Psest_2512 0.31 +1.3 2,690,949 + Psest_2512 0.33 +0.6 2,690,950 - Psest_2512 0.33 -0.6 2,691,015 + Psest_2512 0.40 +1.4 2,691,015 + Psest_2512 0.40 +1.9 2,691,047 - Psest_2512 0.44 +0.1 2,691,052 + Psest_2512 0.45 -0.1 2,691,062 - Psest_2512 0.46 -0.4 2,691,175 - Psest_2512 0.59 -1.3 2,691,175 - Psest_2512 0.59 -0.7 2,691,189 + Psest_2512 0.61 +0.6 2,691,190 - Psest_2512 0.61 +0.4 2,691,190 - Psest_2512 0.61 +0.9 2,691,190 - Psest_2512 0.61 -1.3 2,691,190 - Psest_2512 0.61 +0.1 2,691,240 + Psest_2512 0.67 -0.7 2,691,240 + Psest_2512 0.67 +0.3 2,691,241 - Psest_2512 0.67 -1.6 2,691,241 - Psest_2512 0.67 -1.2 2,691,241 - Psest_2512 0.67 +1.2 2,691,241 - Psest_2512 0.67 +1.9 2,691,264 - Psest_2512 0.70 +0.3 2,691,516 - -1.1 2,691,524 + -1.3 2,691,524 + +0.3 2,691,525 - -1.2 2,691,703 - Psest_2513 0.15 -1.1 2,691,703 - Psest_2513 0.15 -1.3 2,691,975 + Psest_2513 0.80 -0.6 2,691,976 - Psest_2513 0.81 -1.3 2,691,976 - Psest_2513 0.81 -0.9 2,691,976 - Psest_2513 0.81 +0.8 2,692,517 - Psest_2514 0.75 -0.6 2,692,607 + +1.5 2,692,607 + +0.6 2,692,657 + -0.7 2,692,657 + +0.7 2,692,657 + -0.0 2,692,657 + +1.2 2,692,658 - +0.6 2,692,840 + +1.9 2,692,869 + -0.3 2,692,869 + -0.1 2,692,869 + -0.1 2,692,870 - -1.1 2,692,896 + Psest_2515 0.12 -0.8 2,692,896 + Psest_2515 0.12 +2.9 2,692,896 + Psest_2515 0.12 -1.0 2,692,896 + Psest_2515 0.12 +0.6 2,692,896 + Psest_2515 0.12 +0.9 2,692,896 + Psest_2515 0.12 -0.4 2,692,896 + Psest_2515 0.12 -0.8 2,692,896 + Psest_2515 0.12 -0.1 2,692,896 + Psest_2515 0.12 +0.3 2,692,897 - Psest_2515 0.13 +1.6 2,692,897 - Psest_2515 0.13 -1.6 2,692,897 - Psest_2515 0.13 -0.2 2,692,897 - Psest_2515 0.13 +0.9 2,692,897 - Psest_2515 0.13 -0.2 2,692,897 - Psest_2515 0.13 -1.9 2,693,047 + Psest_2515 0.29 +1.1
Or see this region's nucleotide sequence