Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_04410 and IAI47_04415 are separated by 235 nucleotides IAI47_04415 and IAI47_04420 are separated by 280 nucleotides
IAI47_04410: IAI47_04410 - bifunctional chorismate mutase/prephenate dehydratase, at 924,076 to 925,251
_04410
IAI47_04415: IAI47_04415 - ribosome-associated translation inhibitor RaiA, at 925,487 to 925,822
_04415
IAI47_04420: IAI47_04420 - outer membrane protein assembly factor BamD, at 926,103 to 926,834
_04420
Position (kb) 925
926 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 924.496 kb on + strand, within IAI47_04410 at 924.496 kb on + strand, within IAI47_04410 at 924.497 kb on - strand, within IAI47_04410 at 924.497 kb on - strand, within IAI47_04410 at 924.497 kb on - strand, within IAI47_04410 at 924.497 kb on - strand, within IAI47_04410 at 924.497 kb on - strand, within IAI47_04410 at 924.497 kb on - strand, within IAI47_04410 at 924.497 kb on - strand, within IAI47_04410 at 924.497 kb on - strand, within IAI47_04410 at 924.510 kb on + strand, within IAI47_04410 at 924.510 kb on + strand, within IAI47_04410 at 924.510 kb on + strand, within IAI47_04410 at 924.510 kb on + strand, within IAI47_04410 at 924.510 kb on + strand, within IAI47_04410 at 924.510 kb on + strand, within IAI47_04410 at 924.510 kb on + strand, within IAI47_04410 at 924.510 kb on + strand, within IAI47_04410 at 924.510 kb on + strand, within IAI47_04410 at 924.510 kb on + strand, within IAI47_04410 at 924.511 kb on - strand, within IAI47_04410 at 924.511 kb on - strand, within IAI47_04410 at 924.511 kb on - strand, within IAI47_04410 at 924.511 kb on - strand, within IAI47_04410 at 924.511 kb on - strand, within IAI47_04410 at 924.511 kb on - strand, within IAI47_04410 at 924.511 kb on - strand, within IAI47_04410 at 924.552 kb on + strand, within IAI47_04410 at 924.552 kb on + strand, within IAI47_04410 at 924.552 kb on + strand, within IAI47_04410 at 924.553 kb on - strand, within IAI47_04410 at 924.553 kb on - strand, within IAI47_04410 at 924.561 kb on + strand, within IAI47_04410 at 924.562 kb on - strand, within IAI47_04410 at 924.562 kb on - strand, within IAI47_04410 at 924.563 kb on + strand, within IAI47_04410 at 924.563 kb on + strand, within IAI47_04410 at 924.564 kb on - strand, within IAI47_04410 at 924.564 kb on - strand, within IAI47_04410 at 924.591 kb on + strand, within IAI47_04410 at 924.592 kb on - strand, within IAI47_04410 at 924.594 kb on + strand, within IAI47_04410 at 924.594 kb on + strand, within IAI47_04410 at 924.594 kb on + strand, within IAI47_04410 at 924.594 kb on + strand, within IAI47_04410 at 924.595 kb on - strand, within IAI47_04410 at 924.595 kb on - strand, within IAI47_04410 at 924.595 kb on - strand, within IAI47_04410 at 924.632 kb on + strand, within IAI47_04410 at 924.632 kb on + strand, within IAI47_04410 at 924.632 kb on + strand, within IAI47_04410 at 924.632 kb on + strand, within IAI47_04410 at 924.632 kb on + strand, within IAI47_04410 at 924.633 kb on - strand, within IAI47_04410 at 924.633 kb on - strand, within IAI47_04410 at 924.633 kb on - strand, within IAI47_04410 at 924.633 kb on - strand, within IAI47_04410 at 924.633 kb on - strand, within IAI47_04410 at 924.690 kb on + strand, within IAI47_04410 at 924.690 kb on + strand, within IAI47_04410 at 924.690 kb on + strand, within IAI47_04410 at 924.690 kb on + strand, within IAI47_04410 at 924.690 kb on + strand, within IAI47_04410 at 924.690 kb on + strand, within IAI47_04410 at 924.691 kb on - strand, within IAI47_04410 at 924.694 kb on + strand, within IAI47_04410 at 924.694 kb on + strand, within IAI47_04410 at 924.694 kb on + strand, within IAI47_04410 at 924.694 kb on + strand, within IAI47_04410 at 924.694 kb on + strand, within IAI47_04410 at 924.694 kb on + strand, within IAI47_04410 at 924.694 kb on + strand, within IAI47_04410 at 924.694 kb on + strand, within IAI47_04410 at 924.694 kb on + strand, within IAI47_04410 at 924.694 kb on + strand, within IAI47_04410 at 924.694 kb on + strand, within IAI47_04410 at 924.695 kb on - strand, within IAI47_04410 at 924.695 kb on - strand, within IAI47_04410 at 924.695 kb on - strand, within IAI47_04410 at 924.695 kb on - strand, within IAI47_04410 at 924.695 kb on - strand, within IAI47_04410 at 924.695 kb on - strand, within IAI47_04410 at 924.695 kb on - strand, within IAI47_04410 at 924.695 kb on - strand, within IAI47_04410 at 924.695 kb on - strand, within IAI47_04410 at 924.695 kb on - strand, within IAI47_04410 at 924.695 kb on - strand, within IAI47_04410 at 924.695 kb on - strand, within IAI47_04410 at 924.714 kb on + strand, within IAI47_04410 at 924.714 kb on + strand, within IAI47_04410 at 924.714 kb on + strand, within IAI47_04410 at 924.714 kb on + strand, within IAI47_04410 at 924.715 kb on - strand, within IAI47_04410 at 924.715 kb on - strand, within IAI47_04410 at 924.715 kb on - strand, within IAI47_04410 at 924.715 kb on - strand, within IAI47_04410 at 924.715 kb on - strand, within IAI47_04410 at 924.743 kb on + strand, within IAI47_04410 at 924.743 kb on + strand, within IAI47_04410 at 924.743 kb on + strand, within IAI47_04410 at 924.744 kb on - strand, within IAI47_04410 at 924.744 kb on - strand, within IAI47_04410 at 924.794 kb on + strand, within IAI47_04410 at 924.884 kb on + strand, within IAI47_04410 at 924.884 kb on + strand, within IAI47_04410 at 924.884 kb on + strand, within IAI47_04410 at 924.884 kb on + strand, within IAI47_04410 at 924.884 kb on + strand, within IAI47_04410 at 924.885 kb on - strand, within IAI47_04410 at 924.885 kb on - strand, within IAI47_04410 at 924.885 kb on - strand, within IAI47_04410 at 925.037 kb on + strand, within IAI47_04410 at 925.037 kb on + strand, within IAI47_04410 at 925.037 kb on + strand, within IAI47_04410 at 925.037 kb on + strand, within IAI47_04410 at 925.037 kb on + strand, within IAI47_04410 at 925.037 kb on + strand, within IAI47_04410 at 925.037 kb on + strand, within IAI47_04410 at 925.037 kb on + strand, within IAI47_04410 at 925.038 kb on - strand, within IAI47_04410 at 925.038 kb on - strand, within IAI47_04410 at 925.038 kb on - strand, within IAI47_04410 at 925.062 kb on + strand, within IAI47_04410 at 925.069 kb on + strand, within IAI47_04410 at 925.069 kb on + strand, within IAI47_04410 at 925.069 kb on + strand, within IAI47_04410 at 925.070 kb on - strand, within IAI47_04410 at 925.070 kb on - strand, within IAI47_04410 at 925.215 kb on + strand at 925.216 kb on - strand at 925.216 kb on - strand at 925.251 kb on + strand at 925.264 kb on + strand at 925.264 kb on + strand at 925.264 kb on + strand at 925.264 kb on + strand at 925.264 kb on + strand at 925.264 kb on + strand at 925.264 kb on + strand at 925.264 kb on + strand at 925.264 kb on + strand at 925.265 kb on - strand at 925.265 kb on - strand at 925.265 kb on - strand at 925.265 kb on - strand at 925.265 kb on - strand at 925.265 kb on - strand at 925.265 kb on - strand at 925.265 kb on - strand at 925.265 kb on - strand at 925.265 kb on - strand at 925.265 kb on - strand at 925.269 kb on + strand at 925.269 kb on + strand at 925.269 kb on + strand at 925.270 kb on - strand at 925.270 kb on - strand at 925.270 kb on - strand at 925.270 kb on - strand at 925.270 kb on - strand at 925.270 kb on - strand at 925.339 kb on + strand at 925.339 kb on + strand at 925.361 kb on + strand at 925.362 kb on - strand at 925.400 kb on + strand at 925.400 kb on + strand at 925.400 kb on + strand at 925.400 kb on + strand at 925.429 kb on + strand at 925.429 kb on + strand at 925.430 kb on - strand at 925.435 kb on + strand at 925.435 kb on + strand at 925.435 kb on + strand at 925.435 kb on + strand at 925.442 kb on + strand at 925.442 kb on + strand at 925.443 kb on - strand at 925.455 kb on + strand at 925.456 kb on - strand at 925.456 kb on - strand at 925.511 kb on - strand at 925.552 kb on + strand, within IAI47_04415 at 925.552 kb on + strand, within IAI47_04415 at 925.552 kb on + strand, within IAI47_04415 at 925.553 kb on - strand, within IAI47_04415 at 925.553 kb on - strand, within IAI47_04415 at 925.553 kb on - strand, within IAI47_04415 at 925.553 kb on - strand, within IAI47_04415 at 925.553 kb on - strand, within IAI47_04415 at 925.609 kb on + strand, within IAI47_04415 at 925.611 kb on + strand, within IAI47_04415 at 925.611 kb on + strand, within IAI47_04415 at 925.611 kb on + strand, within IAI47_04415 at 925.611 kb on + strand, within IAI47_04415 at 925.611 kb on + strand, within IAI47_04415 at 925.611 kb on + strand, within IAI47_04415 at 925.611 kb on + strand, within IAI47_04415 at 925.611 kb on + strand, within IAI47_04415 at 925.612 kb on - strand, within IAI47_04415 at 925.612 kb on - strand, within IAI47_04415 at 925.612 kb on - strand, within IAI47_04415 at 925.612 kb on - strand, within IAI47_04415 at 925.612 kb on - strand, within IAI47_04415 at 925.615 kb on + strand, within IAI47_04415 at 925.615 kb on + strand, within IAI47_04415 at 925.615 kb on + strand, within IAI47_04415 at 925.615 kb on + strand, within IAI47_04415 at 925.615 kb on + strand, within IAI47_04415 at 925.616 kb on - strand, within IAI47_04415 at 925.616 kb on - strand at 925.616 kb on - strand, within IAI47_04415 at 925.616 kb on - strand, within IAI47_04415 at 925.616 kb on - strand, within IAI47_04415 at 925.660 kb on + strand, within IAI47_04415 at 925.661 kb on - strand, within IAI47_04415 at 925.663 kb on + strand, within IAI47_04415 at 925.663 kb on + strand, within IAI47_04415 at 925.663 kb on + strand, within IAI47_04415 at 925.663 kb on + strand, within IAI47_04415 at 925.663 kb on + strand, within IAI47_04415 at 925.664 kb on - strand, within IAI47_04415 at 925.708 kb on + strand, within IAI47_04415 at 925.709 kb on - strand, within IAI47_04415 at 925.717 kb on + strand, within IAI47_04415 at 925.802 kb on - strand at 925.808 kb on - strand at 925.823 kb on + strand at 925.823 kb on + strand at 925.824 kb on - strand at 925.824 kb on - strand at 925.824 kb on - strand at 925.829 kb on + strand at 925.865 kb on + strand at 925.866 kb on - strand at 925.882 kb on - strand at 925.926 kb on + strand at 925.926 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 924,496 + IAI47_04410 0.36 +0.4 924,496 + IAI47_04410 0.36 +0.1 924,497 - IAI47_04410 0.36 -0.7 924,497 - IAI47_04410 0.36 -0.7 924,497 - IAI47_04410 0.36 -0.0 924,497 - IAI47_04410 0.36 +0.8 924,497 - IAI47_04410 0.36 -0.8 924,497 - IAI47_04410 0.36 +1.3 924,497 - IAI47_04410 0.36 +0.5 924,497 - IAI47_04410 0.36 -1.6 924,510 + IAI47_04410 0.37 -2.0 924,510 + IAI47_04410 0.37 +0.4 924,510 + IAI47_04410 0.37 +0.7 924,510 + IAI47_04410 0.37 +0.1 924,510 + IAI47_04410 0.37 +1.4 924,510 + IAI47_04410 0.37 -2.1 924,510 + IAI47_04410 0.37 +2.5 924,510 + IAI47_04410 0.37 -0.9 924,510 + IAI47_04410 0.37 +0.3 924,510 + IAI47_04410 0.37 -0.7 924,511 - IAI47_04410 0.37 -0.8 924,511 - IAI47_04410 0.37 -0.6 924,511 - IAI47_04410 0.37 -0.1 924,511 - IAI47_04410 0.37 +0.0 924,511 - IAI47_04410 0.37 +0.1 924,511 - IAI47_04410 0.37 -1.2 924,511 - IAI47_04410 0.37 +0.8 924,552 + IAI47_04410 0.40 -0.6 924,552 + IAI47_04410 0.40 +1.4 924,552 + IAI47_04410 0.40 +0.2 924,553 - IAI47_04410 0.41 -0.9 924,553 - IAI47_04410 0.41 +0.4 924,561 + IAI47_04410 0.41 +0.2 924,562 - IAI47_04410 0.41 -0.6 924,562 - IAI47_04410 0.41 +0.5 924,563 + IAI47_04410 0.41 -0.2 924,563 + IAI47_04410 0.41 +1.2 924,564 - IAI47_04410 0.41 -1.4 924,564 - IAI47_04410 0.41 -0.9 924,591 + IAI47_04410 0.44 -0.6 924,592 - IAI47_04410 0.44 -0.3 924,594 + IAI47_04410 0.44 -0.4 924,594 + IAI47_04410 0.44 -1.0 924,594 + IAI47_04410 0.44 +0.3 924,594 + IAI47_04410 0.44 +2.0 924,595 - IAI47_04410 0.44 +1.9 924,595 - IAI47_04410 0.44 +0.4 924,595 - IAI47_04410 0.44 +0.2 924,632 + IAI47_04410 0.47 +0.4 924,632 + IAI47_04410 0.47 +1.2 924,632 + IAI47_04410 0.47 +0.9 924,632 + IAI47_04410 0.47 -0.7 924,632 + IAI47_04410 0.47 -0.2 924,633 - IAI47_04410 0.47 -0.6 924,633 - IAI47_04410 0.47 +1.2 924,633 - IAI47_04410 0.47 +0.7 924,633 - IAI47_04410 0.47 +1.7 924,633 - IAI47_04410 0.47 -0.8 924,690 + IAI47_04410 0.52 -0.8 924,690 + IAI47_04410 0.52 +0.2 924,690 + IAI47_04410 0.52 -0.7 924,690 + IAI47_04410 0.52 -0.9 924,690 + IAI47_04410 0.52 +0.2 924,690 + IAI47_04410 0.52 +1.8 924,691 - IAI47_04410 0.52 -0.6 924,694 + IAI47_04410 0.53 +0.9 924,694 + IAI47_04410 0.53 +1.2 924,694 + IAI47_04410 0.53 -0.8 924,694 + IAI47_04410 0.53 -0.1 924,694 + IAI47_04410 0.53 -1.3 924,694 + IAI47_04410 0.53 -0.0 924,694 + IAI47_04410 0.53 +0.3 924,694 + IAI47_04410 0.53 -1.0 924,694 + IAI47_04410 0.53 +0.1 924,694 + IAI47_04410 0.53 -1.6 924,694 + IAI47_04410 0.53 +0.2 924,695 - IAI47_04410 0.53 +0.7 924,695 - IAI47_04410 0.53 +0.8 924,695 - IAI47_04410 0.53 -0.6 924,695 - IAI47_04410 0.53 -0.2 924,695 - IAI47_04410 0.53 -0.6 924,695 - IAI47_04410 0.53 -1.1 924,695 - IAI47_04410 0.53 +1.2 924,695 - IAI47_04410 0.53 +0.7 924,695 - IAI47_04410 0.53 +0.5 924,695 - IAI47_04410 0.53 +1.6 924,695 - IAI47_04410 0.53 -0.1 924,695 - IAI47_04410 0.53 -0.6 924,714 + IAI47_04410 0.54 -1.2 924,714 + IAI47_04410 0.54 +1.5 924,714 + IAI47_04410 0.54 -0.5 924,714 + IAI47_04410 0.54 -0.1 924,715 - IAI47_04410 0.54 -1.9 924,715 - IAI47_04410 0.54 -1.3 924,715 - IAI47_04410 0.54 -0.2 924,715 - IAI47_04410 0.54 +2.8 924,715 - IAI47_04410 0.54 -0.2 924,743 + IAI47_04410 0.57 +0.3 924,743 + IAI47_04410 0.57 +1.3 924,743 + IAI47_04410 0.57 -0.2 924,744 - IAI47_04410 0.57 +0.7 924,744 - IAI47_04410 0.57 -0.7 924,794 + IAI47_04410 0.61 +0.4 924,884 + IAI47_04410 0.69 -0.8 924,884 + IAI47_04410 0.69 -0.4 924,884 + IAI47_04410 0.69 +2.8 924,884 + IAI47_04410 0.69 -1.5 924,884 + IAI47_04410 0.69 +0.8 924,885 - IAI47_04410 0.69 -0.8 924,885 - IAI47_04410 0.69 -1.1 924,885 - IAI47_04410 0.69 +1.2 925,037 + IAI47_04410 0.82 -2.7 925,037 + IAI47_04410 0.82 -0.4 925,037 + IAI47_04410 0.82 +0.4 925,037 + IAI47_04410 0.82 +0.4 925,037 + IAI47_04410 0.82 -0.4 925,037 + IAI47_04410 0.82 +0.1 925,037 + IAI47_04410 0.82 +0.1 925,037 + IAI47_04410 0.82 -0.6 925,038 - IAI47_04410 0.82 +0.8 925,038 - IAI47_04410 0.82 -1.3 925,038 - IAI47_04410 0.82 -1.4 925,062 + IAI47_04410 0.84 -0.2 925,069 + IAI47_04410 0.84 -2.1 925,069 + IAI47_04410 0.84 -0.8 925,069 + IAI47_04410 0.84 -1.5 925,070 - IAI47_04410 0.85 -0.7 925,070 - IAI47_04410 0.85 +0.4 925,215 + -0.9 925,216 - +1.4 925,216 - +0.8 925,251 + -1.2 925,264 + +1.0 925,264 + +0.1 925,264 + +1.1 925,264 + -0.4 925,264 + -1.4 925,264 + -2.3 925,264 + -0.3 925,264 + +1.0 925,264 + +0.8 925,265 - +1.5 925,265 - +2.9 925,265 - +1.2 925,265 - -0.5 925,265 - +3.5 925,265 - +1.3 925,265 - -0.1 925,265 - +1.2 925,265 - +1.1 925,265 - +1.6 925,265 - +0.5 925,269 + +0.2 925,269 + -0.6 925,269 + -1.3 925,270 - -0.0 925,270 - -0.8 925,270 - +0.4 925,270 - +1.8 925,270 - +0.2 925,270 - -0.9 925,339 + +1.0 925,339 + +0.2 925,361 + +1.8 925,362 - +1.8 925,400 + -1.6 925,400 + +0.5 925,400 + +0.1 925,400 + -1.4 925,429 + +1.5 925,429 + +2.1 925,430 - -0.6 925,435 + +0.3 925,435 + +0.3 925,435 + -1.8 925,435 + +0.2 925,442 + +1.9 925,442 + +0.2 925,443 - +2.6 925,455 + +2.8 925,456 - -0.8 925,456 - -0.2 925,511 - +0.1 925,552 + IAI47_04415 0.19 +0.8 925,552 + IAI47_04415 0.19 +2.3 925,552 + IAI47_04415 0.19 -0.8 925,553 - IAI47_04415 0.20 +1.4 925,553 - IAI47_04415 0.20 +0.5 925,553 - IAI47_04415 0.20 +0.7 925,553 - IAI47_04415 0.20 +0.6 925,553 - IAI47_04415 0.20 +0.8 925,609 + IAI47_04415 0.36 -0.1 925,611 + IAI47_04415 0.37 +0.6 925,611 + IAI47_04415 0.37 +0.1 925,611 + IAI47_04415 0.37 +1.4 925,611 + IAI47_04415 0.37 +1.3 925,611 + IAI47_04415 0.37 +1.7 925,611 + IAI47_04415 0.37 -0.5 925,611 + IAI47_04415 0.37 +2.3 925,611 + IAI47_04415 0.37 +1.4 925,612 - IAI47_04415 0.37 +0.0 925,612 - IAI47_04415 0.37 +3.0 925,612 - IAI47_04415 0.37 +0.3 925,612 - IAI47_04415 0.37 +0.8 925,612 - IAI47_04415 0.37 +1.2 925,615 + IAI47_04415 0.38 +1.8 925,615 + IAI47_04415 0.38 +0.2 925,615 + IAI47_04415 0.38 +0.7 925,615 + IAI47_04415 0.38 +3.5 925,615 + IAI47_04415 0.38 +1.6 925,616 - IAI47_04415 0.38 +0.2 925,616 - +0.4 925,616 - IAI47_04415 0.38 +1.0 925,616 - IAI47_04415 0.38 -0.2 925,616 - IAI47_04415 0.38 -1.4 925,660 + IAI47_04415 0.51 -0.0 925,661 - IAI47_04415 0.52 +1.0 925,663 + IAI47_04415 0.52 +0.7 925,663 + IAI47_04415 0.52 +0.2 925,663 + IAI47_04415 0.52 +1.3 925,663 + IAI47_04415 0.52 -0.6 925,663 + IAI47_04415 0.52 +1.2 925,664 - IAI47_04415 0.53 +2.6 925,708 + IAI47_04415 0.66 -0.5 925,709 - IAI47_04415 0.66 +0.3 925,717 + IAI47_04415 0.68 +0.6 925,802 - -0.7 925,808 - +0.7 925,823 + -1.9 925,823 + +2.5 925,824 - -0.0 925,824 - -0.2 925,824 - -0.5 925,829 + -1.0 925,865 + -0.9 925,866 - -0.8 925,882 - -0.2 925,926 + +1.5 925,926 + +1.2
Or see this region's nucleotide sequence