Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_01850 and IAI47_01855 are separated by 391 nucleotides IAI47_01855 and IAI47_01860 are separated by 71 nucleotides
IAI47_01850: IAI47_01850 - intracellular growth attenuator family protein, at 405,136 to 407,271
_01850
IAI47_01855: IAI47_01855 - ADP compounds hydrolase NudE, at 407,663 to 408,226
_01855
IAI47_01860: IAI47_01860 - peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA, at 408,298 to 410,847
_01860
Position (kb) 407
408
409 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 407.336 kb on - strand at 407.338 kb on - strand at 407.338 kb on - strand at 407.338 kb on - strand at 407.360 kb on - strand at 407.360 kb on - strand at 407.361 kb on + strand at 407.361 kb on + strand at 407.362 kb on - strand at 407.362 kb on - strand at 407.362 kb on - strand at 407.362 kb on - strand at 407.362 kb on - strand at 407.363 kb on + strand at 407.363 kb on + strand at 407.363 kb on + strand at 407.364 kb on - strand at 407.364 kb on - strand at 407.404 kb on + strand at 407.404 kb on + strand at 407.405 kb on - strand at 407.405 kb on - strand at 407.405 kb on - strand at 407.405 kb on - strand at 407.405 kb on - strand at 407.405 kb on - strand at 407.405 kb on - strand at 407.405 kb on - strand at 407.405 kb on - strand at 407.405 kb on - strand at 407.405 kb on - strand at 407.405 kb on - strand at 407.405 kb on - strand at 407.405 kb on - strand at 407.405 kb on - strand at 407.407 kb on - strand at 407.407 kb on - strand at 407.407 kb on - strand at 407.425 kb on - strand at 407.466 kb on + strand at 407.467 kb on - strand at 407.467 kb on - strand at 407.467 kb on - strand at 407.470 kb on + strand at 407.471 kb on - strand at 407.479 kb on + strand at 407.480 kb on - strand at 407.490 kb on + strand at 407.500 kb on + strand at 407.500 kb on + strand at 407.508 kb on - strand at 407.531 kb on + strand at 407.535 kb on + strand at 407.536 kb on - strand at 407.545 kb on + strand at 407.545 kb on + strand at 407.545 kb on + strand at 407.546 kb on - strand at 407.546 kb on - strand at 407.611 kb on + strand at 407.615 kb on + strand at 407.615 kb on + strand at 407.615 kb on + strand at 407.615 kb on + strand at 407.615 kb on + strand at 407.615 kb on + strand at 407.615 kb on + strand at 407.615 kb on + strand at 407.615 kb on + strand at 407.615 kb on + strand at 407.615 kb on + strand at 407.616 kb on - strand at 407.616 kb on - strand at 407.616 kb on - strand at 407.616 kb on - strand at 407.616 kb on - strand at 407.620 kb on + strand at 407.620 kb on + strand at 407.620 kb on + strand at 407.620 kb on + strand at 407.620 kb on + strand at 407.620 kb on + strand at 407.620 kb on + strand at 407.620 kb on + strand at 407.620 kb on + strand at 407.621 kb on - strand at 407.621 kb on - strand at 407.621 kb on - strand at 407.634 kb on + strand at 407.634 kb on + strand at 407.634 kb on + strand at 407.635 kb on - strand at 407.635 kb on - strand at 407.635 kb on - strand at 407.635 kb on - strand at 407.648 kb on + strand at 407.662 kb on + strand at 407.663 kb on - strand at 407.695 kb on + strand at 407.695 kb on + strand at 407.695 kb on + strand at 407.695 kb on + strand at 407.696 kb on - strand at 407.774 kb on + strand, within IAI47_01855 at 407.774 kb on + strand, within IAI47_01855 at 407.774 kb on + strand, within IAI47_01855 at 407.774 kb on + strand, within IAI47_01855 at 407.774 kb on + strand, within IAI47_01855 at 407.775 kb on - strand, within IAI47_01855 at 407.775 kb on - strand, within IAI47_01855 at 407.775 kb on - strand, within IAI47_01855 at 407.824 kb on + strand, within IAI47_01855 at 407.824 kb on + strand, within IAI47_01855 at 407.855 kb on + strand, within IAI47_01855 at 407.856 kb on - strand, within IAI47_01855 at 407.856 kb on - strand, within IAI47_01855 at 407.856 kb on - strand, within IAI47_01855 at 407.876 kb on + strand, within IAI47_01855 at 407.876 kb on + strand, within IAI47_01855 at 407.877 kb on - strand, within IAI47_01855 at 407.877 kb on - strand, within IAI47_01855 at 408.014 kb on + strand, within IAI47_01855 at 408.014 kb on + strand, within IAI47_01855 at 408.015 kb on - strand, within IAI47_01855 at 408.015 kb on - strand, within IAI47_01855 at 408.015 kb on - strand, within IAI47_01855 at 408.034 kb on + strand, within IAI47_01855 at 408.035 kb on - strand, within IAI47_01855 at 408.035 kb on - strand, within IAI47_01855 at 408.056 kb on + strand, within IAI47_01855 at 408.056 kb on + strand, within IAI47_01855 at 408.056 kb on + strand, within IAI47_01855 at 408.057 kb on - strand, within IAI47_01855 at 408.057 kb on - strand, within IAI47_01855 at 408.057 kb on - strand, within IAI47_01855 at 408.057 kb on - strand, within IAI47_01855 at 408.057 kb on - strand, within IAI47_01855 at 408.098 kb on - strand, within IAI47_01855 at 408.098 kb on - strand, within IAI47_01855 at 408.163 kb on + strand, within IAI47_01855 at 408.163 kb on + strand, within IAI47_01855 at 408.163 kb on + strand, within IAI47_01855 at 408.164 kb on - strand, within IAI47_01855 at 408.173 kb on - strand at 408.182 kb on - strand at 408.238 kb on + strand at 408.238 kb on + strand at 408.238 kb on + strand at 408.238 kb on + strand at 408.238 kb on + strand at 408.239 kb on - strand at 408.239 kb on - strand at 408.239 kb on - strand at 408.274 kb on + strand at 408.285 kb on - strand at 408.285 kb on - strand at 408.330 kb on + strand at 408.331 kb on - strand at 408.331 kb on - strand at 408.333 kb on + strand at 408.333 kb on + strand at 408.333 kb on + strand at 408.333 kb on + strand at 408.333 kb on + strand at 408.334 kb on - strand at 408.334 kb on - strand at 408.334 kb on - strand at 408.334 kb on - strand at 408.334 kb on - strand at 408.334 kb on - strand at 408.334 kb on - strand at 408.340 kb on - strand at 408.359 kb on + strand at 408.389 kb on + strand at 408.389 kb on + strand at 408.389 kb on + strand at 408.390 kb on - strand at 408.390 kb on - strand at 408.390 kb on - strand at 408.390 kb on - strand at 408.390 kb on - strand at 408.490 kb on + strand at 408.490 kb on + strand at 408.490 kb on + strand at 408.490 kb on + strand at 408.557 kb on + strand, within IAI47_01860 at 408.558 kb on - strand, within IAI47_01860 at 408.565 kb on + strand, within IAI47_01860 at 408.565 kb on + strand, within IAI47_01860 at 408.566 kb on - strand, within IAI47_01860 at 408.595 kb on - strand, within IAI47_01860 at 408.595 kb on - strand, within IAI47_01860 at 408.609 kb on + strand, within IAI47_01860 at 408.609 kb on + strand, within IAI47_01860 at 408.649 kb on + strand, within IAI47_01860 at 408.649 kb on + strand, within IAI47_01860 at 408.649 kb on + strand, within IAI47_01860 at 408.650 kb on - strand, within IAI47_01860 at 408.650 kb on - strand, within IAI47_01860 at 408.650 kb on - strand, within IAI47_01860 at 408.650 kb on - strand, within IAI47_01860 at 408.650 kb on - strand, within IAI47_01860 at 408.718 kb on - strand, within IAI47_01860 at 408.723 kb on + strand, within IAI47_01860 at 408.723 kb on + strand, within IAI47_01860 at 408.723 kb on + strand, within IAI47_01860 at 408.723 kb on + strand, within IAI47_01860 at 408.724 kb on - strand, within IAI47_01860 at 408.724 kb on - strand, within IAI47_01860 at 408.729 kb on + strand, within IAI47_01860 at 408.729 kb on + strand, within IAI47_01860 at 408.783 kb on + strand, within IAI47_01860 at 408.783 kb on + strand, within IAI47_01860 at 408.783 kb on + strand, within IAI47_01860 at 408.783 kb on + strand, within IAI47_01860 at 408.784 kb on - strand, within IAI47_01860 at 408.784 kb on - strand, within IAI47_01860 at 408.784 kb on - strand, within IAI47_01860 at 408.784 kb on - strand, within IAI47_01860 at 408.784 kb on - strand, within IAI47_01860 at 408.784 kb on - strand, within IAI47_01860 at 408.784 kb on - strand, within IAI47_01860 at 408.784 kb on - strand, within IAI47_01860 at 408.784 kb on - strand, within IAI47_01860 at 408.784 kb on - strand, within IAI47_01860 at 408.784 kb on - strand, within IAI47_01860 at 408.784 kb on - strand, within IAI47_01860 at 408.786 kb on + strand, within IAI47_01860 at 408.787 kb on - strand, within IAI47_01860 at 408.787 kb on - strand, within IAI47_01860 at 408.787 kb on - strand, within IAI47_01860 at 408.787 kb on - strand, within IAI47_01860 at 408.787 kb on - strand, within IAI47_01860 at 408.825 kb on + strand, within IAI47_01860 at 408.825 kb on + strand, within IAI47_01860 at 408.826 kb on - strand, within IAI47_01860 at 408.826 kb on - strand, within IAI47_01860 at 408.826 kb on - strand, within IAI47_01860 at 408.845 kb on + strand, within IAI47_01860 at 408.845 kb on + strand, within IAI47_01860 at 408.845 kb on + strand, within IAI47_01860 at 408.845 kb on + strand, within IAI47_01860 at 408.845 kb on + strand, within IAI47_01860 at 408.845 kb on + strand, within IAI47_01860 at 408.846 kb on - strand, within IAI47_01860 at 408.846 kb on - strand, within IAI47_01860 at 408.846 kb on - strand, within IAI47_01860 at 408.846 kb on - strand, within IAI47_01860 at 408.846 kb on - strand, within IAI47_01860 at 408.846 kb on - strand, within IAI47_01860 at 408.921 kb on + strand, within IAI47_01860 at 408.921 kb on + strand, within IAI47_01860 at 408.921 kb on + strand, within IAI47_01860 at 408.922 kb on - strand, within IAI47_01860 at 408.922 kb on - strand, within IAI47_01860 at 408.922 kb on - strand, within IAI47_01860 at 408.922 kb on - strand, within IAI47_01860 at 408.922 kb on - strand, within IAI47_01860 at 408.922 kb on - strand, within IAI47_01860 at 408.922 kb on - strand, within IAI47_01860 at 408.986 kb on + strand, within IAI47_01860 at 408.986 kb on + strand, within IAI47_01860 at 408.986 kb on + strand, within IAI47_01860 at 408.986 kb on + strand, within IAI47_01860 at 408.987 kb on - strand, within IAI47_01860 at 408.987 kb on - strand, within IAI47_01860 at 408.987 kb on - strand, within IAI47_01860 at 409.072 kb on - strand, within IAI47_01860 at 409.079 kb on + strand, within IAI47_01860 at 409.079 kb on + strand, within IAI47_01860 at 409.079 kb on + strand, within IAI47_01860 at 409.080 kb on - strand, within IAI47_01860 at 409.080 kb on - strand, within IAI47_01860 at 409.080 kb on - strand, within IAI47_01860 at 409.080 kb on - strand, within IAI47_01860 at 409.080 kb on - strand, within IAI47_01860 at 409.080 kb on - strand, within IAI47_01860 at 409.119 kb on - strand, within IAI47_01860 at 409.119 kb on - strand, within IAI47_01860 at 409.135 kb on + strand, within IAI47_01860 at 409.136 kb on - strand, within IAI47_01860 at 409.136 kb on - strand, within IAI47_01860 at 409.218 kb on - strand, within IAI47_01860 at 409.218 kb on - strand, within IAI47_01860
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 407,336 - +0.4 407,338 - +0.3 407,338 - +2.3 407,338 - +2.0 407,360 - -0.6 407,360 - +2.4 407,361 + -3.7 407,361 + -2.3 407,362 - -1.4 407,362 - -0.1 407,362 - -0.4 407,362 - +0.1 407,362 - +2.2 407,363 + -0.7 407,363 + +1.2 407,363 + +0.1 407,364 - +0.7 407,364 - +1.2 407,404 + -2.5 407,404 + -0.7 407,405 - +0.7 407,405 - +2.3 407,405 - +0.7 407,405 - -0.4 407,405 - +0.6 407,405 - +0.9 407,405 - +0.3 407,405 - -0.1 407,405 - +2.8 407,405 - -0.3 407,405 - +0.6 407,405 - -0.0 407,405 - -0.5 407,405 - +0.4 407,405 - -0.6 407,407 - -0.0 407,407 - +3.2 407,407 - +2.0 407,425 - +0.9 407,466 + -2.0 407,467 - +0.1 407,467 - +1.7 407,467 - +1.4 407,470 + -2.1 407,471 - +0.8 407,479 + -3.7 407,480 - +0.0 407,490 + -1.2 407,500 + +0.1 407,500 + -0.8 407,508 - +0.7 407,531 + +0.0 407,535 + +1.4 407,536 - -0.4 407,545 + -0.8 407,545 + -0.1 407,545 + +0.2 407,546 - -0.2 407,546 - +0.8 407,611 + -0.0 407,615 + +1.6 407,615 + +0.2 407,615 + -0.5 407,615 + -1.1 407,615 + -0.1 407,615 + -0.2 407,615 + +0.3 407,615 + +0.8 407,615 + +2.2 407,615 + -0.2 407,615 + -0.2 407,616 - +2.9 407,616 - +0.3 407,616 - -0.0 407,616 - +2.0 407,616 - -0.6 407,620 + -0.3 407,620 + +1.2 407,620 + +1.1 407,620 + +1.7 407,620 + -0.3 407,620 + +0.7 407,620 + -0.0 407,620 + +1.2 407,620 + +2.0 407,621 - +1.7 407,621 - +0.9 407,621 - -0.4 407,634 + -0.2 407,634 + +0.2 407,634 + -2.8 407,635 - -0.1 407,635 - -0.1 407,635 - -1.8 407,635 - +2.0 407,648 + +0.2 407,662 + -0.9 407,663 - -0.7 407,695 + -0.3 407,695 + +0.1 407,695 + +0.2 407,695 + +0.0 407,696 - -1.6 407,774 + IAI47_01855 0.20 -0.2 407,774 + IAI47_01855 0.20 -1.2 407,774 + IAI47_01855 0.20 +2.2 407,774 + IAI47_01855 0.20 +0.3 407,774 + IAI47_01855 0.20 -2.4 407,775 - IAI47_01855 0.20 -1.3 407,775 - IAI47_01855 0.20 +1.5 407,775 - IAI47_01855 0.20 +0.6 407,824 + IAI47_01855 0.29 -1.4 407,824 + IAI47_01855 0.29 -0.0 407,855 + IAI47_01855 0.34 -1.3 407,856 - IAI47_01855 0.34 -1.2 407,856 - IAI47_01855 0.34 -0.6 407,856 - IAI47_01855 0.34 +1.6 407,876 + IAI47_01855 0.38 -1.8 407,876 + IAI47_01855 0.38 -0.5 407,877 - IAI47_01855 0.38 -1.0 407,877 - IAI47_01855 0.38 -0.6 408,014 + IAI47_01855 0.62 -0.1 408,014 + IAI47_01855 0.62 -0.2 408,015 - IAI47_01855 0.62 -1.6 408,015 - IAI47_01855 0.62 +0.2 408,015 - IAI47_01855 0.62 +1.8 408,034 + IAI47_01855 0.66 +0.2 408,035 - IAI47_01855 0.66 -1.8 408,035 - IAI47_01855 0.66 -0.2 408,056 + IAI47_01855 0.70 -0.3 408,056 + IAI47_01855 0.70 -2.7 408,056 + IAI47_01855 0.70 -0.6 408,057 - IAI47_01855 0.70 -1.9 408,057 - IAI47_01855 0.70 -0.9 408,057 - IAI47_01855 0.70 -0.3 408,057 - IAI47_01855 0.70 -1.2 408,057 - IAI47_01855 0.70 +0.9 408,098 - IAI47_01855 0.77 +0.6 408,098 - IAI47_01855 0.77 -1.4 408,163 + IAI47_01855 0.89 +0.7 408,163 + IAI47_01855 0.89 -0.3 408,163 + IAI47_01855 0.89 -0.1 408,164 - IAI47_01855 0.89 -0.2 408,173 - -1.4 408,182 - -0.7 408,238 + -0.6 408,238 + -0.3 408,238 + -0.2 408,238 + +0.4 408,238 + -1.2 408,239 - -0.1 408,239 - +0.2 408,239 - +0.3 408,274 + +0.7 408,285 - -0.7 408,285 - -0.7 408,330 + -0.2 408,331 - +0.3 408,331 - -0.3 408,333 + -0.4 408,333 + -0.3 408,333 + +0.5 408,333 + +0.3 408,333 + -0.3 408,334 - -0.5 408,334 - -2.4 408,334 - -0.5 408,334 - -2.0 408,334 - +0.2 408,334 - -2.2 408,334 - +0.4 408,340 - +0.8 408,359 + -2.2 408,389 + -0.9 408,389 + -1.7 408,389 + -3.2 408,390 - -3.1 408,390 - +0.5 408,390 - -0.3 408,390 - +1.1 408,390 - -1.9 408,490 + -1.0 408,490 + -1.0 408,490 + -1.2 408,490 + +1.4 408,557 + IAI47_01860 0.10 -0.7 408,558 - IAI47_01860 0.10 -0.3 408,565 + IAI47_01860 0.10 -2.0 408,565 + IAI47_01860 0.10 +0.7 408,566 - IAI47_01860 0.11 -0.4 408,595 - IAI47_01860 0.12 +0.0 408,595 - IAI47_01860 0.12 +0.9 408,609 + IAI47_01860 0.12 -1.7 408,609 + IAI47_01860 0.12 -1.1 408,649 + IAI47_01860 0.14 -0.9 408,649 + IAI47_01860 0.14 -0.2 408,649 + IAI47_01860 0.14 -0.3 408,650 - IAI47_01860 0.14 -1.7 408,650 - IAI47_01860 0.14 -1.3 408,650 - IAI47_01860 0.14 -0.6 408,650 - IAI47_01860 0.14 -0.7 408,650 - IAI47_01860 0.14 -0.1 408,718 - IAI47_01860 0.16 -1.7 408,723 + IAI47_01860 0.17 +1.2 408,723 + IAI47_01860 0.17 +1.2 408,723 + IAI47_01860 0.17 +1.2 408,723 + IAI47_01860 0.17 +0.9 408,724 - IAI47_01860 0.17 -1.2 408,724 - IAI47_01860 0.17 -1.0 408,729 + IAI47_01860 0.17 -1.6 408,729 + IAI47_01860 0.17 -0.3 408,783 + IAI47_01860 0.19 +0.4 408,783 + IAI47_01860 0.19 -0.9 408,783 + IAI47_01860 0.19 +0.8 408,783 + IAI47_01860 0.19 -0.0 408,784 - IAI47_01860 0.19 -1.6 408,784 - IAI47_01860 0.19 -1.3 408,784 - IAI47_01860 0.19 +0.6 408,784 - IAI47_01860 0.19 -1.8 408,784 - IAI47_01860 0.19 -1.5 408,784 - IAI47_01860 0.19 -2.4 408,784 - IAI47_01860 0.19 +0.0 408,784 - IAI47_01860 0.19 -2.9 408,784 - IAI47_01860 0.19 -1.4 408,784 - IAI47_01860 0.19 -1.3 408,784 - IAI47_01860 0.19 -0.9 408,784 - IAI47_01860 0.19 +0.5 408,786 + IAI47_01860 0.19 -1.1 408,787 - IAI47_01860 0.19 +1.0 408,787 - IAI47_01860 0.19 -0.5 408,787 - IAI47_01860 0.19 -1.0 408,787 - IAI47_01860 0.19 -0.9 408,787 - IAI47_01860 0.19 -1.0 408,825 + IAI47_01860 0.21 -1.6 408,825 + IAI47_01860 0.21 -0.1 408,826 - IAI47_01860 0.21 +0.1 408,826 - IAI47_01860 0.21 -1.3 408,826 - IAI47_01860 0.21 -1.1 408,845 + IAI47_01860 0.21 -1.7 408,845 + IAI47_01860 0.21 +0.3 408,845 + IAI47_01860 0.21 -0.7 408,845 + IAI47_01860 0.21 -2.8 408,845 + IAI47_01860 0.21 -0.2 408,845 + IAI47_01860 0.21 -1.0 408,846 - IAI47_01860 0.21 -2.2 408,846 - IAI47_01860 0.21 -1.5 408,846 - IAI47_01860 0.21 -0.5 408,846 - IAI47_01860 0.21 -0.9 408,846 - IAI47_01860 0.21 -1.0 408,846 - IAI47_01860 0.21 -0.5 408,921 + IAI47_01860 0.24 +1.4 408,921 + IAI47_01860 0.24 +0.6 408,921 + IAI47_01860 0.24 +0.3 408,922 - IAI47_01860 0.24 -1.0 408,922 - IAI47_01860 0.24 +1.0 408,922 - IAI47_01860 0.24 -2.7 408,922 - IAI47_01860 0.24 +0.1 408,922 - IAI47_01860 0.24 +1.0 408,922 - IAI47_01860 0.24 +0.1 408,922 - IAI47_01860 0.24 -0.7 408,986 + IAI47_01860 0.27 -2.3 408,986 + IAI47_01860 0.27 -1.1 408,986 + IAI47_01860 0.27 +1.0 408,986 + IAI47_01860 0.27 +1.0 408,987 - IAI47_01860 0.27 +0.3 408,987 - IAI47_01860 0.27 +0.3 408,987 - IAI47_01860 0.27 -1.5 409,072 - IAI47_01860 0.30 -1.8 409,079 + IAI47_01860 0.31 -0.3 409,079 + IAI47_01860 0.31 -0.8 409,079 + IAI47_01860 0.31 -0.5 409,080 - IAI47_01860 0.31 -1.6 409,080 - IAI47_01860 0.31 +0.6 409,080 - IAI47_01860 0.31 -0.8 409,080 - IAI47_01860 0.31 -0.7 409,080 - IAI47_01860 0.31 -1.9 409,080 - IAI47_01860 0.31 +0.3 409,119 - IAI47_01860 0.32 -0.7 409,119 - IAI47_01860 0.32 -3.3 409,135 + IAI47_01860 0.33 -2.5 409,136 - IAI47_01860 0.33 -2.3 409,136 - IAI47_01860 0.33 +0.7 409,218 - IAI47_01860 0.36 +0.3 409,218 - IAI47_01860 0.36 -1.6
Or see this region's nucleotide sequence