Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20255 and IAI47_20260 are separated by 201 nucleotides IAI47_20260 and IAI47_20265 are separated by 16 nucleotides IAI47_20265 and IAI47_20270 overlap by 1 nucleotides
IAI47_20255: IAI47_20255 - alpha/beta fold hydrolase, at 284,758 to 285,618
_20255
IAI47_20260: IAI47_20260 - GFA family protein, at 285,820 to 286,230
_20260
IAI47_20265: IAI47_20265 - DUF1641 domain-containing protein, at 286,247 to 286,732
_20265
IAI47_20270: IAI47_20270 - formate dehydrogenase subunit alpha, at 286,732 to 289,704
_20270
Position (kb) 285
286
287 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 284.822 kb on + strand at 284.822 kb on + strand at 284.823 kb on - strand at 284.826 kb on + strand at 284.827 kb on - strand at 284.866 kb on - strand, within IAI47_20255 at 284.866 kb on - strand, within IAI47_20255 at 284.987 kb on + strand, within IAI47_20255 at 284.988 kb on - strand, within IAI47_20255 at 284.988 kb on - strand, within IAI47_20255 at 285.010 kb on + strand, within IAI47_20255 at 285.045 kb on + strand, within IAI47_20255 at 285.045 kb on + strand, within IAI47_20255 at 285.045 kb on + strand, within IAI47_20255 at 285.046 kb on - strand, within IAI47_20255 at 285.046 kb on - strand, within IAI47_20255 at 285.159 kb on + strand, within IAI47_20255 at 285.159 kb on + strand, within IAI47_20255 at 285.160 kb on - strand, within IAI47_20255 at 285.160 kb on - strand, within IAI47_20255 at 285.160 kb on - strand, within IAI47_20255 at 285.160 kb on - strand, within IAI47_20255 at 285.165 kb on + strand, within IAI47_20255 at 285.165 kb on + strand, within IAI47_20255 at 285.165 kb on + strand, within IAI47_20255 at 285.166 kb on - strand, within IAI47_20255 at 285.310 kb on + strand, within IAI47_20255 at 285.316 kb on + strand, within IAI47_20255 at 285.316 kb on + strand, within IAI47_20255 at 285.316 kb on + strand, within IAI47_20255 at 285.316 kb on + strand, within IAI47_20255 at 285.316 kb on + strand, within IAI47_20255 at 285.316 kb on + strand, within IAI47_20255 at 285.317 kb on - strand, within IAI47_20255 at 285.317 kb on - strand, within IAI47_20255 at 285.317 kb on - strand, within IAI47_20255 at 285.317 kb on - strand, within IAI47_20255 at 285.317 kb on - strand, within IAI47_20255 at 285.372 kb on + strand, within IAI47_20255 at 285.372 kb on + strand, within IAI47_20255 at 285.437 kb on + strand, within IAI47_20255 at 285.438 kb on - strand, within IAI47_20255 at 285.438 kb on - strand, within IAI47_20255 at 285.472 kb on - strand, within IAI47_20255 at 285.534 kb on + strand at 285.535 kb on - strand at 285.606 kb on + strand at 285.606 kb on + strand at 285.607 kb on - strand at 285.607 kb on - strand at 285.610 kb on + strand at 285.610 kb on + strand at 285.610 kb on + strand at 285.611 kb on - strand at 285.611 kb on - strand at 285.644 kb on + strand at 285.645 kb on - strand at 285.645 kb on - strand at 285.664 kb on + strand at 285.665 kb on - strand at 285.693 kb on - strand at 285.696 kb on + strand at 285.697 kb on - strand at 285.697 kb on - strand at 285.697 kb on - strand at 285.761 kb on + strand at 285.804 kb on + strand at 285.865 kb on + strand, within IAI47_20260 at 285.865 kb on + strand, within IAI47_20260 at 285.865 kb on + strand, within IAI47_20260 at 285.865 kb on + strand, within IAI47_20260 at 285.865 kb on + strand at 285.865 kb on + strand, within IAI47_20260 at 285.865 kb on + strand, within IAI47_20260 at 285.866 kb on - strand, within IAI47_20260 at 285.866 kb on - strand, within IAI47_20260 at 285.866 kb on - strand, within IAI47_20260 at 285.866 kb on - strand, within IAI47_20260 at 285.866 kb on - strand, within IAI47_20260 at 285.866 kb on - strand, within IAI47_20260 at 285.866 kb on - strand, within IAI47_20260 at 285.867 kb on + strand, within IAI47_20260 at 285.868 kb on - strand, within IAI47_20260 at 285.878 kb on + strand, within IAI47_20260 at 285.891 kb on + strand, within IAI47_20260 at 285.942 kb on - strand, within IAI47_20260 at 285.998 kb on - strand, within IAI47_20260 at 285.998 kb on - strand, within IAI47_20260 at 286.009 kb on - strand, within IAI47_20260 at 286.009 kb on - strand, within IAI47_20260 at 286.027 kb on + strand, within IAI47_20260 at 286.028 kb on - strand, within IAI47_20260 at 286.028 kb on - strand, within IAI47_20260 at 286.028 kb on - strand, within IAI47_20260 at 286.028 kb on - strand, within IAI47_20260 at 286.028 kb on - strand, within IAI47_20260 at 286.028 kb on - strand, within IAI47_20260 at 286.052 kb on - strand, within IAI47_20260 at 286.052 kb on - strand, within IAI47_20260 at 286.054 kb on - strand, within IAI47_20260 at 286.096 kb on - strand, within IAI47_20260 at 286.096 kb on - strand, within IAI47_20260 at 286.158 kb on + strand, within IAI47_20260 at 286.211 kb on - strand at 286.211 kb on - strand at 286.211 kb on - strand at 286.211 kb on - strand at 286.211 kb on - strand at 286.211 kb on - strand at 286.211 kb on - strand at 286.211 kb on - strand at 286.212 kb on + strand at 286.213 kb on - strand at 286.245 kb on + strand at 286.245 kb on + strand at 286.245 kb on + strand at 286.245 kb on + strand at 286.245 kb on + strand at 286.245 kb on + strand at 286.245 kb on + strand at 286.245 kb on + strand at 286.245 kb on + strand at 286.248 kb on + strand at 286.248 kb on + strand at 286.248 kb on + strand at 286.249 kb on - strand at 286.249 kb on - strand at 286.249 kb on - strand at 286.372 kb on + strand, within IAI47_20265 at 286.372 kb on + strand, within IAI47_20265 at 286.372 kb on + strand, within IAI47_20265 at 286.373 kb on - strand, within IAI47_20265 at 286.373 kb on - strand, within IAI47_20265 at 286.373 kb on - strand, within IAI47_20265 at 286.373 kb on - strand, within IAI47_20265 at 286.376 kb on + strand, within IAI47_20265 at 286.597 kb on + strand, within IAI47_20265 at 286.597 kb on + strand, within IAI47_20265 at 286.598 kb on - strand, within IAI47_20265 at 286.714 kb on - strand at 286.714 kb on - strand at 286.864 kb on - strand at 286.913 kb on + strand at 286.913 kb on + strand at 286.914 kb on - strand at 286.914 kb on - strand at 286.914 kb on - strand at 286.914 kb on - strand at 286.914 kb on - strand at 286.914 kb on - strand at 286.963 kb on - strand at 286.974 kb on + strand at 286.974 kb on + strand at 286.975 kb on - strand at 287.022 kb on + strand at 287.022 kb on + strand at 287.025 kb on + strand at 287.025 kb on + strand at 287.026 kb on - strand at 287.026 kb on - strand at 287.026 kb on - strand at 287.026 kb on - strand at 287.062 kb on - strand, within IAI47_20270 at 287.133 kb on + strand, within IAI47_20270 at 287.134 kb on - strand, within IAI47_20270 at 287.225 kb on + strand, within IAI47_20270 at 287.225 kb on + strand, within IAI47_20270
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 284,822 + +1.6 284,822 + -0.2 284,823 - +2.1 284,826 + -0.5 284,827 - +1.2 284,866 - IAI47_20255 0.13 +2.3 284,866 - IAI47_20255 0.13 -0.2 284,987 + IAI47_20255 0.27 -0.2 284,988 - IAI47_20255 0.27 -2.2 284,988 - IAI47_20255 0.27 +0.7 285,010 + IAI47_20255 0.29 +3.0 285,045 + IAI47_20255 0.33 +0.4 285,045 + IAI47_20255 0.33 +0.9 285,045 + IAI47_20255 0.33 +1.3 285,046 - IAI47_20255 0.33 -0.7 285,046 - IAI47_20255 0.33 +0.1 285,159 + IAI47_20255 0.47 +1.3 285,159 + IAI47_20255 0.47 +0.3 285,160 - IAI47_20255 0.47 +0.7 285,160 - IAI47_20255 0.47 +0.3 285,160 - IAI47_20255 0.47 +1.0 285,160 - IAI47_20255 0.47 +1.4 285,165 + IAI47_20255 0.47 -0.3 285,165 + IAI47_20255 0.47 +0.3 285,165 + IAI47_20255 0.47 +0.9 285,166 - IAI47_20255 0.47 +1.1 285,310 + IAI47_20255 0.64 -0.3 285,316 + IAI47_20255 0.65 +0.1 285,316 + IAI47_20255 0.65 +0.9 285,316 + IAI47_20255 0.65 +0.2 285,316 + IAI47_20255 0.65 -0.8 285,316 + IAI47_20255 0.65 -1.7 285,316 + IAI47_20255 0.65 +1.7 285,317 - IAI47_20255 0.65 -0.1 285,317 - IAI47_20255 0.65 +0.3 285,317 - IAI47_20255 0.65 +0.3 285,317 - IAI47_20255 0.65 -1.0 285,317 - IAI47_20255 0.65 +0.4 285,372 + IAI47_20255 0.71 +0.3 285,372 + IAI47_20255 0.71 +0.2 285,437 + IAI47_20255 0.79 +1.3 285,438 - IAI47_20255 0.79 -1.0 285,438 - IAI47_20255 0.79 +0.0 285,472 - IAI47_20255 0.83 -0.1 285,534 + +0.2 285,535 - -0.4 285,606 + +3.3 285,606 + -1.0 285,607 - -1.6 285,607 - +1.0 285,610 + -0.9 285,610 + +0.5 285,610 + +0.0 285,611 - +1.1 285,611 - +0.7 285,644 + -0.5 285,645 - +1.3 285,645 - -0.2 285,664 + +0.1 285,665 - +0.5 285,693 - +0.0 285,696 + -1.2 285,697 - +0.3 285,697 - -0.6 285,697 - -0.0 285,761 + +1.0 285,804 + -0.7 285,865 + IAI47_20260 0.11 +0.2 285,865 + IAI47_20260 0.11 -0.4 285,865 + IAI47_20260 0.11 -0.2 285,865 + IAI47_20260 0.11 -2.1 285,865 + +0.8 285,865 + IAI47_20260 0.11 +0.9 285,865 + IAI47_20260 0.11 -0.7 285,866 - IAI47_20260 0.11 +1.0 285,866 - IAI47_20260 0.11 -0.6 285,866 - IAI47_20260 0.11 -1.6 285,866 - IAI47_20260 0.11 -0.9 285,866 - IAI47_20260 0.11 +0.6 285,866 - IAI47_20260 0.11 -1.1 285,866 - IAI47_20260 0.11 -0.7 285,867 + IAI47_20260 0.11 -0.8 285,868 - IAI47_20260 0.12 -0.3 285,878 + IAI47_20260 0.14 +2.0 285,891 + IAI47_20260 0.17 +1.1 285,942 - IAI47_20260 0.30 -0.8 285,998 - IAI47_20260 0.43 -0.2 285,998 - IAI47_20260 0.43 -0.5 286,009 - IAI47_20260 0.46 -1.2 286,009 - IAI47_20260 0.46 -2.0 286,027 + IAI47_20260 0.50 +0.9 286,028 - IAI47_20260 0.51 +2.0 286,028 - IAI47_20260 0.51 +0.2 286,028 - IAI47_20260 0.51 +0.7 286,028 - IAI47_20260 0.51 +0.3 286,028 - IAI47_20260 0.51 +0.0 286,028 - IAI47_20260 0.51 -1.1 286,052 - IAI47_20260 0.56 -0.5 286,052 - IAI47_20260 0.56 -2.0 286,054 - IAI47_20260 0.57 +0.5 286,096 - IAI47_20260 0.67 +0.9 286,096 - IAI47_20260 0.67 -1.5 286,158 + IAI47_20260 0.82 +1.8 286,211 - +0.8 286,211 - +0.1 286,211 - +0.3 286,211 - +0.4 286,211 - +0.5 286,211 - -2.2 286,211 - +0.2 286,211 - -0.2 286,212 + -0.2 286,213 - -1.5 286,245 + -0.4 286,245 + +2.3 286,245 + +0.8 286,245 + -1.5 286,245 + -1.7 286,245 + +0.0 286,245 + -0.4 286,245 + -0.2 286,245 + +0.4 286,248 + +1.7 286,248 + +0.2 286,248 + +0.6 286,249 - +0.1 286,249 - +1.7 286,249 - +0.8 286,372 + IAI47_20265 0.26 +1.2 286,372 + IAI47_20265 0.26 +0.6 286,372 + IAI47_20265 0.26 +0.1 286,373 - IAI47_20265 0.26 -0.1 286,373 - IAI47_20265 0.26 +0.6 286,373 - IAI47_20265 0.26 +0.9 286,373 - IAI47_20265 0.26 -0.4 286,376 + IAI47_20265 0.27 -0.2 286,597 + IAI47_20265 0.72 -1.3 286,597 + IAI47_20265 0.72 +0.3 286,598 - IAI47_20265 0.72 +2.4 286,714 - +1.3 286,714 - +0.1 286,864 - +1.4 286,913 + +1.0 286,913 + -1.5 286,914 - +1.4 286,914 - -0.5 286,914 - -0.3 286,914 - -0.7 286,914 - +0.9 286,914 - -1.8 286,963 - +0.7 286,974 + -0.3 286,974 + +0.0 286,975 - +1.7 287,022 + +2.3 287,022 + +0.0 287,025 + +0.7 287,025 + -0.9 287,026 - -1.3 287,026 - +1.2 287,026 - +0.9 287,026 - +1.3 287,062 - IAI47_20270 0.11 +0.2 287,133 + IAI47_20270 0.13 +1.0 287,134 - IAI47_20270 0.14 +1.7 287,225 + IAI47_20270 0.17 -0.8 287,225 + IAI47_20270 0.17 +1.2
Or see this region's nucleotide sequence