Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_18915 and IAI47_18920 are separated by 391 nucleotides IAI47_18920 and IAI47_18925 overlap by 47 nucleotides IAI47_18925 and IAI47_18930 are separated by 96 nucleotides
IAI47_18915: IAI47_18915 - GNAT family N-acetyltransferase, at 20,758 to 21,312
_18915
IAI47_18920: IAI47_18920 - TetR/AcrR family transcriptional regulator, at 21,704 to 22,291
_18920
IAI47_18925: IAI47_18925 - LysR family transcriptional regulator, at 22,245 to 23,138
_18925
IAI47_18930: IAI47_18930 - carboxymuconolactone decarboxylase family protein, at 23,235 to 23,540
_18930
Position (kb) 21
22
23 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 20.720 kb on + strand at 20.720 kb on + strand at 20.721 kb on - strand at 20.721 kb on - strand at 20.721 kb on - strand at 20.728 kb on + strand at 20.739 kb on + strand at 20.743 kb on + strand at 20.743 kb on + strand at 20.743 kb on + strand at 20.743 kb on + strand at 20.743 kb on + strand at 20.744 kb on - strand at 20.744 kb on - strand at 20.744 kb on - strand at 20.780 kb on + strand at 20.780 kb on + strand at 20.780 kb on + strand at 20.785 kb on + strand at 20.793 kb on + strand at 20.793 kb on + strand at 20.793 kb on + strand at 20.798 kb on + strand at 20.798 kb on + strand at 20.798 kb on + strand at 20.799 kb on - strand at 20.830 kb on + strand, within IAI47_18915 at 20.830 kb on + strand, within IAI47_18915 at 20.831 kb on - strand, within IAI47_18915 at 20.831 kb on - strand, within IAI47_18915 at 20.831 kb on - strand, within IAI47_18915 at 20.831 kb on - strand, within IAI47_18915 at 20.831 kb on - strand, within IAI47_18915 at 20.850 kb on + strand, within IAI47_18915 at 20.850 kb on + strand, within IAI47_18915 at 20.850 kb on + strand, within IAI47_18915 at 20.850 kb on + strand, within IAI47_18915 at 20.851 kb on - strand, within IAI47_18915 at 20.851 kb on - strand, within IAI47_18915 at 20.851 kb on - strand, within IAI47_18915 at 20.851 kb on - strand, within IAI47_18915 at 20.857 kb on - strand, within IAI47_18915 at 20.887 kb on + strand, within IAI47_18915 at 20.887 kb on + strand, within IAI47_18915 at 20.887 kb on + strand, within IAI47_18915 at 20.887 kb on + strand, within IAI47_18915 at 20.887 kb on + strand, within IAI47_18915 at 20.887 kb on + strand, within IAI47_18915 at 20.887 kb on + strand, within IAI47_18915 at 20.888 kb on - strand, within IAI47_18915 at 20.901 kb on + strand, within IAI47_18915 at 20.901 kb on + strand, within IAI47_18915 at 20.901 kb on + strand, within IAI47_18915 at 20.902 kb on - strand, within IAI47_18915 at 20.902 kb on - strand, within IAI47_18915 at 20.902 kb on - strand, within IAI47_18915 at 20.910 kb on + strand, within IAI47_18915 at 20.910 kb on + strand, within IAI47_18915 at 20.911 kb on - strand, within IAI47_18915 at 20.911 kb on - strand, within IAI47_18915 at 20.913 kb on - strand, within IAI47_18915 at 20.913 kb on - strand, within IAI47_18915 at 20.913 kb on - strand, within IAI47_18915 at 20.924 kb on - strand, within IAI47_18915 at 20.924 kb on - strand, within IAI47_18915 at 20.954 kb on + strand, within IAI47_18915 at 20.954 kb on + strand, within IAI47_18915 at 20.955 kb on - strand, within IAI47_18915 at 21.000 kb on - strand, within IAI47_18915 at 21.000 kb on - strand, within IAI47_18915 at 21.034 kb on - strand, within IAI47_18915 at 21.076 kb on + strand, within IAI47_18915 at 21.102 kb on + strand, within IAI47_18915 at 21.103 kb on - strand, within IAI47_18915 at 21.103 kb on - strand, within IAI47_18915 at 21.103 kb on - strand, within IAI47_18915 at 21.138 kb on + strand, within IAI47_18915 at 21.139 kb on - strand, within IAI47_18915 at 21.139 kb on - strand, within IAI47_18915 at 21.150 kb on - strand, within IAI47_18915 at 21.201 kb on - strand, within IAI47_18915 at 21.253 kb on + strand, within IAI47_18915 at 21.253 kb on + strand, within IAI47_18915 at 21.253 kb on + strand, within IAI47_18915 at 21.253 kb on + strand, within IAI47_18915 at 21.253 kb on + strand, within IAI47_18915 at 21.253 kb on + strand, within IAI47_18915 at 21.253 kb on + strand, within IAI47_18915 at 21.253 kb on + strand, within IAI47_18915 at 21.253 kb on + strand, within IAI47_18915 at 21.253 kb on + strand, within IAI47_18915 at 21.253 kb on + strand, within IAI47_18915 at 21.254 kb on - strand, within IAI47_18915 at 21.254 kb on - strand, within IAI47_18915 at 21.254 kb on - strand, within IAI47_18915 at 21.254 kb on - strand, within IAI47_18915 at 21.254 kb on - strand, within IAI47_18915 at 21.254 kb on - strand, within IAI47_18915 at 21.255 kb on + strand, within IAI47_18915 at 21.256 kb on - strand, within IAI47_18915 at 21.321 kb on + strand at 21.321 kb on + strand at 21.321 kb on + strand at 21.321 kb on + strand at 21.321 kb on + strand at 21.321 kb on + strand at 21.321 kb on + strand at 21.321 kb on + strand at 21.321 kb on + strand at 21.322 kb on - strand at 21.322 kb on - strand at 21.322 kb on - strand at 21.322 kb on - strand at 21.323 kb on + strand at 21.323 kb on + strand at 21.323 kb on + strand at 21.323 kb on + strand at 21.324 kb on - strand at 21.324 kb on - strand at 21.416 kb on + strand at 21.417 kb on - strand at 21.421 kb on + strand at 21.430 kb on + strand at 21.431 kb on - strand at 21.446 kb on + strand at 21.446 kb on + strand at 21.446 kb on + strand at 21.446 kb on + strand at 21.447 kb on - strand at 21.447 kb on - strand at 21.447 kb on - strand at 21.453 kb on - strand at 21.496 kb on + strand at 21.497 kb on - strand at 21.499 kb on - strand at 21.536 kb on + strand at 21.536 kb on + strand at 21.536 kb on + strand at 21.536 kb on + strand at 21.537 kb on - strand at 21.564 kb on - strand at 21.579 kb on + strand at 21.580 kb on - strand at 21.594 kb on + strand at 21.594 kb on + strand at 21.595 kb on - strand at 21.630 kb on + strand at 21.667 kb on + strand at 21.745 kb on + strand at 21.745 kb on + strand at 21.746 kb on - strand at 21.746 kb on - strand at 21.746 kb on - strand at 21.746 kb on - strand at 21.763 kb on + strand, within IAI47_18920 at 21.763 kb on + strand, within IAI47_18920 at 21.763 kb on + strand, within IAI47_18920 at 21.764 kb on - strand, within IAI47_18920 at 21.780 kb on + strand, within IAI47_18920 at 21.903 kb on + strand, within IAI47_18920 at 21.937 kb on + strand, within IAI47_18920 at 21.937 kb on + strand, within IAI47_18920 at 21.938 kb on - strand, within IAI47_18920 at 21.961 kb on + strand, within IAI47_18920 at 21.961 kb on + strand, within IAI47_18920 at 21.962 kb on - strand, within IAI47_18920 at 21.962 kb on - strand, within IAI47_18920 at 21.962 kb on - strand, within IAI47_18920 at 22.000 kb on + strand, within IAI47_18920 at 22.000 kb on + strand, within IAI47_18920 at 22.001 kb on - strand, within IAI47_18920 at 22.079 kb on + strand, within IAI47_18920 at 22.079 kb on + strand, within IAI47_18920 at 22.079 kb on + strand, within IAI47_18920 at 22.080 kb on - strand, within IAI47_18920 at 22.080 kb on - strand, within IAI47_18920 at 22.080 kb on - strand, within IAI47_18920 at 22.080 kb on - strand, within IAI47_18920 at 22.080 kb on - strand, within IAI47_18920 at 22.080 kb on - strand, within IAI47_18920 at 22.174 kb on + strand, within IAI47_18920 at 22.174 kb on + strand, within IAI47_18920 at 22.175 kb on - strand, within IAI47_18920 at 22.175 kb on - strand, within IAI47_18920 at 22.204 kb on + strand, within IAI47_18920 at 22.246 kb on + strand at 22.247 kb on - strand at 22.310 kb on + strand at 22.310 kb on + strand at 22.360 kb on + strand, within IAI47_18925 at 22.360 kb on + strand, within IAI47_18925 at 22.409 kb on + strand, within IAI47_18925 at 22.409 kb on + strand, within IAI47_18925 at 22.409 kb on + strand, within IAI47_18925 at 22.410 kb on - strand, within IAI47_18925 at 22.410 kb on - strand, within IAI47_18925 at 22.410 kb on - strand, within IAI47_18925 at 22.410 kb on - strand, within IAI47_18925 at 22.410 kb on - strand, within IAI47_18925 at 22.431 kb on + strand, within IAI47_18925 at 22.431 kb on + strand, within IAI47_18925 at 22.432 kb on - strand, within IAI47_18925 at 22.432 kb on - strand, within IAI47_18925 at 22.432 kb on - strand, within IAI47_18925 at 22.432 kb on - strand, within IAI47_18925 at 22.434 kb on - strand, within IAI47_18925 at 22.434 kb on - strand, within IAI47_18925 at 22.729 kb on - strand, within IAI47_18925 at 22.729 kb on - strand, within IAI47_18925 at 22.841 kb on + strand, within IAI47_18925 at 22.841 kb on + strand, within IAI47_18925 at 22.842 kb on - strand, within IAI47_18925 at 22.842 kb on - strand, within IAI47_18925 at 22.842 kb on - strand, within IAI47_18925 at 22.845 kb on + strand, within IAI47_18925 at 22.845 kb on + strand, within IAI47_18925 at 22.845 kb on + strand, within IAI47_18925 at 22.845 kb on + strand, within IAI47_18925 at 22.846 kb on - strand, within IAI47_18925 at 22.846 kb on - strand, within IAI47_18925 at 22.918 kb on + strand, within IAI47_18925 at 22.919 kb on - strand, within IAI47_18925 at 22.925 kb on + strand, within IAI47_18925 at 22.926 kb on - strand, within IAI47_18925 at 22.926 kb on - strand, within IAI47_18925 at 22.926 kb on - strand, within IAI47_18925 at 23.132 kb on + strand at 23.132 kb on + strand at 23.132 kb on + strand at 23.149 kb on + strand at 23.197 kb on + strand at 23.203 kb on + strand at 23.203 kb on + strand at 23.203 kb on + strand at 23.204 kb on - strand at 23.204 kb on - strand at 23.204 kb on - strand at 23.204 kb on - strand at 23.204 kb on - strand at 23.204 kb on - strand at 23.204 kb on - strand at 23.204 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 20,720 + -1.9 20,720 + +0.1 20,721 - +1.8 20,721 - +0.0 20,721 - -2.1 20,728 + -2.3 20,739 + -0.2 20,743 + +0.2 20,743 + -1.8 20,743 + +0.2 20,743 + -1.1 20,743 + +2.5 20,744 - +2.1 20,744 - -0.8 20,744 - -1.2 20,780 + +1.2 20,780 + -1.6 20,780 + +0.4 20,785 + -1.5 20,793 + +0.5 20,793 + +1.3 20,793 + -1.1 20,798 + +0.6 20,798 + -3.7 20,798 + +0.5 20,799 - +1.7 20,830 + IAI47_18915 0.13 -0.6 20,830 + IAI47_18915 0.13 -1.2 20,831 - IAI47_18915 0.13 -1.0 20,831 - IAI47_18915 0.13 -0.4 20,831 - IAI47_18915 0.13 +0.7 20,831 - IAI47_18915 0.13 +1.6 20,831 - IAI47_18915 0.13 -1.2 20,850 + IAI47_18915 0.17 +0.5 20,850 + IAI47_18915 0.17 +1.6 20,850 + IAI47_18915 0.17 -0.4 20,850 + IAI47_18915 0.17 -2.5 20,851 - IAI47_18915 0.17 +1.5 20,851 - IAI47_18915 0.17 -0.8 20,851 - IAI47_18915 0.17 -0.3 20,851 - IAI47_18915 0.17 +0.4 20,857 - IAI47_18915 0.18 -0.4 20,887 + IAI47_18915 0.23 +4.3 20,887 + IAI47_18915 0.23 -0.3 20,887 + IAI47_18915 0.23 +1.3 20,887 + IAI47_18915 0.23 -0.1 20,887 + IAI47_18915 0.23 +1.1 20,887 + IAI47_18915 0.23 -1.0 20,887 + IAI47_18915 0.23 +0.9 20,888 - IAI47_18915 0.23 -1.3 20,901 + IAI47_18915 0.26 -1.4 20,901 + IAI47_18915 0.26 -0.1 20,901 + IAI47_18915 0.26 -0.3 20,902 - IAI47_18915 0.26 +1.2 20,902 - IAI47_18915 0.26 +0.4 20,902 - IAI47_18915 0.26 +3.1 20,910 + IAI47_18915 0.27 +0.3 20,910 + IAI47_18915 0.27 -0.2 20,911 - IAI47_18915 0.28 -0.2 20,911 - IAI47_18915 0.28 +3.0 20,913 - IAI47_18915 0.28 +2.4 20,913 - IAI47_18915 0.28 +2.1 20,913 - IAI47_18915 0.28 -0.7 20,924 - IAI47_18915 0.30 -0.3 20,924 - IAI47_18915 0.30 +1.1 20,954 + IAI47_18915 0.35 +0.3 20,954 + IAI47_18915 0.35 -1.6 20,955 - IAI47_18915 0.35 -0.0 21,000 - IAI47_18915 0.44 +0.7 21,000 - IAI47_18915 0.44 -1.3 21,034 - IAI47_18915 0.50 -0.5 21,076 + IAI47_18915 0.57 -0.9 21,102 + IAI47_18915 0.62 +0.5 21,103 - IAI47_18915 0.62 +1.1 21,103 - IAI47_18915 0.62 -0.9 21,103 - IAI47_18915 0.62 +0.4 21,138 + IAI47_18915 0.68 -1.8 21,139 - IAI47_18915 0.69 +1.3 21,139 - IAI47_18915 0.69 +0.1 21,150 - IAI47_18915 0.71 +0.7 21,201 - IAI47_18915 0.80 +2.6 21,253 + IAI47_18915 0.89 +1.0 21,253 + IAI47_18915 0.89 -0.4 21,253 + IAI47_18915 0.89 +0.5 21,253 + IAI47_18915 0.89 -1.9 21,253 + IAI47_18915 0.89 +0.0 21,253 + IAI47_18915 0.89 -0.1 21,253 + IAI47_18915 0.89 +0.7 21,253 + IAI47_18915 0.89 -2.1 21,253 + IAI47_18915 0.89 +2.2 21,253 + IAI47_18915 0.89 +0.6 21,253 + IAI47_18915 0.89 +1.0 21,254 - IAI47_18915 0.89 +1.4 21,254 - IAI47_18915 0.89 -1.5 21,254 - IAI47_18915 0.89 -2.5 21,254 - IAI47_18915 0.89 +0.3 21,254 - IAI47_18915 0.89 +0.1 21,254 - IAI47_18915 0.89 +2.7 21,255 + IAI47_18915 0.90 +1.8 21,256 - IAI47_18915 0.90 -2.8 21,321 + -2.5 21,321 + -0.8 21,321 + +0.7 21,321 + -0.8 21,321 + -1.3 21,321 + +0.5 21,321 + -0.2 21,321 + -0.4 21,321 + -0.4 21,322 - +1.6 21,322 - -0.6 21,322 - -1.8 21,322 - +2.2 21,323 + +1.7 21,323 + +1.4 21,323 + +0.3 21,323 + -1.3 21,324 - +0.8 21,324 - -0.3 21,416 + +0.0 21,417 - +1.2 21,421 + +0.7 21,430 + -1.4 21,431 - -0.5 21,446 + +3.3 21,446 + +0.7 21,446 + +0.7 21,446 + +0.4 21,447 - +0.3 21,447 - +0.5 21,447 - +0.4 21,453 - +1.4 21,496 + +0.3 21,497 - +0.7 21,499 - -0.4 21,536 + +1.6 21,536 + -0.9 21,536 + +0.3 21,536 + +1.9 21,537 - +0.6 21,564 - +2.1 21,579 + +0.4 21,580 - +1.9 21,594 + -0.5 21,594 + +0.2 21,595 - -2.9 21,630 + +0.7 21,667 + -0.7 21,745 + -0.7 21,745 + -0.1 21,746 - -2.3 21,746 - -3.1 21,746 - -1.0 21,746 - +3.9 21,763 + IAI47_18920 0.10 -0.1 21,763 + IAI47_18920 0.10 +1.0 21,763 + IAI47_18920 0.10 +0.3 21,764 - IAI47_18920 0.10 -1.9 21,780 + IAI47_18920 0.13 -1.0 21,903 + IAI47_18920 0.34 +0.2 21,937 + IAI47_18920 0.40 -0.1 21,937 + IAI47_18920 0.40 -1.4 21,938 - IAI47_18920 0.40 -1.6 21,961 + IAI47_18920 0.44 +0.4 21,961 + IAI47_18920 0.44 -2.4 21,962 - IAI47_18920 0.44 -1.0 21,962 - IAI47_18920 0.44 -0.3 21,962 - IAI47_18920 0.44 +0.7 22,000 + IAI47_18920 0.50 -0.8 22,000 + IAI47_18920 0.50 -1.3 22,001 - IAI47_18920 0.51 +0.0 22,079 + IAI47_18920 0.64 -2.0 22,079 + IAI47_18920 0.64 -0.4 22,079 + IAI47_18920 0.64 -2.3 22,080 - IAI47_18920 0.64 -0.4 22,080 - IAI47_18920 0.64 +0.1 22,080 - IAI47_18920 0.64 -0.3 22,080 - IAI47_18920 0.64 -0.8 22,080 - IAI47_18920 0.64 -1.4 22,080 - IAI47_18920 0.64 -1.0 22,174 + IAI47_18920 0.80 -1.7 22,174 + IAI47_18920 0.80 +3.5 22,175 - IAI47_18920 0.80 +1.7 22,175 - IAI47_18920 0.80 -1.1 22,204 + IAI47_18920 0.85 -1.6 22,246 + +1.3 22,247 - +1.9 22,310 + +0.2 22,310 + -0.3 22,360 + IAI47_18925 0.13 -0.1 22,360 + IAI47_18925 0.13 +0.0 22,409 + IAI47_18925 0.18 +0.6 22,409 + IAI47_18925 0.18 -0.3 22,409 + IAI47_18925 0.18 +0.8 22,410 - IAI47_18925 0.18 +0.3 22,410 - IAI47_18925 0.18 +1.6 22,410 - IAI47_18925 0.18 -1.8 22,410 - IAI47_18925 0.18 -1.5 22,410 - IAI47_18925 0.18 +1.0 22,431 + IAI47_18925 0.21 -0.7 22,431 + IAI47_18925 0.21 +0.7 22,432 - IAI47_18925 0.21 -0.3 22,432 - IAI47_18925 0.21 -1.1 22,432 - IAI47_18925 0.21 -1.0 22,432 - IAI47_18925 0.21 -1.0 22,434 - IAI47_18925 0.21 +0.0 22,434 - IAI47_18925 0.21 -0.9 22,729 - IAI47_18925 0.54 +0.1 22,729 - IAI47_18925 0.54 -0.3 22,841 + IAI47_18925 0.67 -0.5 22,841 + IAI47_18925 0.67 -1.1 22,842 - IAI47_18925 0.67 -0.8 22,842 - IAI47_18925 0.67 +1.5 22,842 - IAI47_18925 0.67 -2.1 22,845 + IAI47_18925 0.67 -1.2 22,845 + IAI47_18925 0.67 -1.2 22,845 + IAI47_18925 0.67 -1.0 22,845 + IAI47_18925 0.67 +1.9 22,846 - IAI47_18925 0.67 -2.0 22,846 - IAI47_18925 0.67 +1.1 22,918 + IAI47_18925 0.75 -0.7 22,919 - IAI47_18925 0.75 +0.7 22,925 + IAI47_18925 0.76 -0.5 22,926 - IAI47_18925 0.76 +0.7 22,926 - IAI47_18925 0.76 -1.3 22,926 - IAI47_18925 0.76 -1.0 23,132 + -1.3 23,132 + +0.6 23,132 + +0.7 23,149 + +0.9 23,197 + +0.5 23,203 + +1.2 23,203 + +0.4 23,203 + +0.2 23,204 - +0.8 23,204 - -1.8 23,204 - -0.2 23,204 - -0.2 23,204 - +0.5 23,204 - +1.1 23,204 - +0.3 23,204 - +0.5
Or see this region's nucleotide sequence