Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_11780 and IAI47_11785 are separated by 20 nucleotides IAI47_11785 and IAI47_11790 are separated by 2 nucleotides IAI47_11790 and IAI47_11795 overlap by 8 nucleotides
IAI47_11780: IAI47_11780 - fucose isomerase, at 2,498,469 to 2,499,887
_11780
IAI47_11785: IAI47_11785 - FGGY-family carbohydrate kinase, at 2,499,908 to 2,501,395
_11785
IAI47_11790: IAI47_11790 - transketolase family protein, at 2,501,398 to 2,502,342
_11790
IAI47_11795: IAI47_11795 - transketolase, at 2,502,335 to 2,503,165
_11795
Position (kb)
2499
2500
2501
2502 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2498.944 kb on + strand, within IAI47_11780 at 2498.945 kb on - strand, within IAI47_11780 at 2498.945 kb on - strand, within IAI47_11780 at 2499.077 kb on + strand, within IAI47_11780 at 2499.078 kb on - strand, within IAI47_11780 at 2499.083 kb on + strand, within IAI47_11780 at 2499.084 kb on - strand, within IAI47_11780 at 2499.120 kb on + strand, within IAI47_11780 at 2499.120 kb on + strand, within IAI47_11780 at 2499.120 kb on + strand, within IAI47_11780 at 2499.120 kb on + strand, within IAI47_11780 at 2499.121 kb on - strand, within IAI47_11780 at 2499.121 kb on - strand, within IAI47_11780 at 2499.121 kb on - strand, within IAI47_11780 at 2499.121 kb on - strand, within IAI47_11780 at 2499.121 kb on - strand, within IAI47_11780 at 2499.210 kb on - strand, within IAI47_11780 at 2499.302 kb on + strand, within IAI47_11780 at 2499.302 kb on + strand, within IAI47_11780 at 2499.303 kb on - strand, within IAI47_11780 at 2499.304 kb on + strand, within IAI47_11780 at 2499.304 kb on + strand, within IAI47_11780 at 2499.305 kb on - strand, within IAI47_11780 at 2499.305 kb on - strand, within IAI47_11780 at 2499.305 kb on - strand, within IAI47_11780 at 2499.305 kb on - strand, within IAI47_11780 at 2499.305 kb on - strand, within IAI47_11780 at 2499.305 kb on - strand, within IAI47_11780 at 2499.317 kb on + strand, within IAI47_11780 at 2499.317 kb on + strand, within IAI47_11780 at 2499.318 kb on - strand, within IAI47_11780 at 2499.393 kb on - strand, within IAI47_11780 at 2499.467 kb on + strand, within IAI47_11780 at 2499.467 kb on + strand, within IAI47_11780 at 2499.467 kb on + strand, within IAI47_11780 at 2499.467 kb on + strand, within IAI47_11780 at 2499.468 kb on - strand, within IAI47_11780 at 2499.468 kb on - strand, within IAI47_11780 at 2499.472 kb on + strand, within IAI47_11780 at 2499.472 kb on + strand, within IAI47_11780 at 2499.472 kb on + strand, within IAI47_11780 at 2499.473 kb on - strand, within IAI47_11780 at 2499.473 kb on - strand, within IAI47_11780 at 2499.473 kb on - strand, within IAI47_11780 at 2499.542 kb on + strand, within IAI47_11780 at 2499.542 kb on + strand, within IAI47_11780 at 2499.543 kb on - strand, within IAI47_11780 at 2499.543 kb on - strand, within IAI47_11780 at 2499.551 kb on + strand, within IAI47_11780 at 2499.551 kb on + strand, within IAI47_11780 at 2499.552 kb on - strand, within IAI47_11780 at 2499.552 kb on - strand, within IAI47_11780 at 2499.552 kb on - strand, within IAI47_11780 at 2499.635 kb on + strand, within IAI47_11780 at 2499.635 kb on + strand, within IAI47_11780 at 2499.636 kb on - strand, within IAI47_11780 at 2499.721 kb on - strand, within IAI47_11780 at 2499.761 kb on + strand at 2499.820 kb on + strand at 2499.820 kb on + strand at 2499.820 kb on + strand at 2499.821 kb on - strand at 2499.821 kb on - strand at 2499.864 kb on + strand at 2499.865 kb on - strand at 2499.909 kb on + strand at 2499.909 kb on + strand at 2499.910 kb on - strand at 2499.910 kb on - strand at 2499.910 kb on - strand at 2499.955 kb on + strand at 2499.956 kb on - strand at 2499.956 kb on - strand at 2499.957 kb on + strand at 2499.957 kb on + strand at 2499.970 kb on + strand at 2499.971 kb on - strand at 2499.971 kb on - strand at 2500.016 kb on + strand at 2500.027 kb on + strand at 2500.027 kb on + strand at 2500.027 kb on + strand at 2500.028 kb on - strand at 2500.028 kb on - strand at 2500.028 kb on - strand at 2500.142 kb on + strand, within IAI47_11785 at 2500.142 kb on + strand, within IAI47_11785 at 2500.159 kb on + strand, within IAI47_11785 at 2500.159 kb on + strand, within IAI47_11785 at 2500.377 kb on + strand, within IAI47_11785 at 2500.377 kb on + strand, within IAI47_11785 at 2500.378 kb on - strand, within IAI47_11785 at 2500.378 kb on - strand, within IAI47_11785 at 2500.378 kb on - strand, within IAI47_11785 at 2500.450 kb on + strand, within IAI47_11785 at 2500.450 kb on + strand, within IAI47_11785 at 2500.504 kb on - strand, within IAI47_11785 at 2500.602 kb on + strand, within IAI47_11785 at 2500.602 kb on + strand, within IAI47_11785 at 2500.602 kb on + strand, within IAI47_11785 at 2500.602 kb on + strand, within IAI47_11785 at 2500.602 kb on + strand, within IAI47_11785 at 2500.603 kb on - strand, within IAI47_11785 at 2500.603 kb on - strand, within IAI47_11785 at 2500.603 kb on - strand, within IAI47_11785 at 2500.603 kb on - strand, within IAI47_11785 at 2500.603 kb on - strand, within IAI47_11785 at 2500.603 kb on - strand, within IAI47_11785 at 2500.603 kb on - strand, within IAI47_11785 at 2500.603 kb on - strand, within IAI47_11785 at 2500.603 kb on - strand, within IAI47_11785 at 2500.603 kb on - strand, within IAI47_11785 at 2500.603 kb on - strand, within IAI47_11785 at 2500.603 kb on - strand, within IAI47_11785 at 2500.670 kb on - strand, within IAI47_11785 at 2500.687 kb on + strand, within IAI47_11785 at 2500.687 kb on + strand, within IAI47_11785 at 2500.687 kb on + strand, within IAI47_11785 at 2500.688 kb on - strand, within IAI47_11785 at 2500.688 kb on - strand, within IAI47_11785 at 2500.715 kb on + strand, within IAI47_11785 at 2500.715 kb on + strand, within IAI47_11785 at 2500.716 kb on - strand, within IAI47_11785 at 2500.827 kb on + strand, within IAI47_11785 at 2500.827 kb on + strand, within IAI47_11785 at 2500.827 kb on + strand, within IAI47_11785 at 2500.827 kb on + strand, within IAI47_11785 at 2500.827 kb on + strand, within IAI47_11785 at 2500.827 kb on + strand, within IAI47_11785 at 2500.828 kb on - strand, within IAI47_11785 at 2500.828 kb on - strand, within IAI47_11785 at 2500.978 kb on + strand, within IAI47_11785 at 2501.161 kb on + strand, within IAI47_11785 at 2501.161 kb on + strand, within IAI47_11785 at 2501.161 kb on + strand, within IAI47_11785 at 2501.161 kb on + strand, within IAI47_11785 at 2501.162 kb on - strand, within IAI47_11785 at 2501.162 kb on - strand, within IAI47_11785 at 2501.162 kb on - strand, within IAI47_11785 at 2501.228 kb on + strand, within IAI47_11785 at 2501.228 kb on + strand, within IAI47_11785 at 2501.229 kb on - strand, within IAI47_11785 at 2501.292 kb on - strand at 2501.399 kb on + strand at 2501.399 kb on + strand at 2501.399 kb on + strand at 2501.400 kb on - strand at 2501.400 kb on - strand at 2501.400 kb on - strand at 2501.496 kb on - strand, within IAI47_11790 at 2501.496 kb on - strand, within IAI47_11790 at 2501.607 kb on + strand, within IAI47_11790 at 2501.652 kb on + strand, within IAI47_11790 at 2501.816 kb on + strand, within IAI47_11790 at 2501.862 kb on + strand, within IAI47_11790 at 2501.864 kb on + strand, within IAI47_11790 at 2501.864 kb on + strand, within IAI47_11790 at 2501.864 kb on + strand, within IAI47_11790 at 2501.864 kb on + strand, within IAI47_11790 at 2501.864 kb on + strand, within IAI47_11790 at 2501.864 kb on + strand, within IAI47_11790 at 2501.864 kb on + strand, within IAI47_11790 at 2501.865 kb on - strand, within IAI47_11790 at 2501.865 kb on - strand, within IAI47_11790 at 2501.865 kb on - strand, within IAI47_11790 at 2501.865 kb on - strand, within IAI47_11790 at 2501.865 kb on - strand, within IAI47_11790 at 2501.891 kb on + strand, within IAI47_11790 at 2501.892 kb on - strand, within IAI47_11790 at 2501.892 kb on - strand, within IAI47_11790 at 2501.892 kb on - strand, within IAI47_11790 at 2501.933 kb on + strand, within IAI47_11790 at 2501.933 kb on + strand, within IAI47_11790 at 2501.933 kb on + strand, within IAI47_11790 at 2501.933 kb on + strand, within IAI47_11790 at 2501.933 kb on + strand, within IAI47_11790 at 2501.933 kb on + strand, within IAI47_11790 at 2501.933 kb on + strand, within IAI47_11790 at 2501.933 kb on + strand, within IAI47_11790 at 2501.933 kb on + strand, within IAI47_11790 at 2501.934 kb on - strand, within IAI47_11790 at 2501.934 kb on - strand, within IAI47_11790 at 2501.934 kb on - strand, within IAI47_11790 at 2501.934 kb on - strand, within IAI47_11790 at 2502.008 kb on + strand, within IAI47_11790 at 2502.009 kb on - strand, within IAI47_11790 at 2502.056 kb on + strand, within IAI47_11790 at 2502.056 kb on + strand, within IAI47_11790 at 2502.057 kb on - strand, within IAI47_11790 at 2502.057 kb on - strand, within IAI47_11790 at 2502.115 kb on - strand, within IAI47_11790 at 2502.192 kb on + strand, within IAI47_11790 at 2502.192 kb on + strand, within IAI47_11790 at 2502.192 kb on + strand, within IAI47_11790 at 2502.193 kb on - strand, within IAI47_11790 at 2502.193 kb on - strand, within IAI47_11790 at 2502.193 kb on - strand, within IAI47_11790 at 2502.216 kb on + strand, within IAI47_11790 at 2502.216 kb on + strand, within IAI47_11790 at 2502.219 kb on + strand, within IAI47_11790 at 2502.219 kb on + strand, within IAI47_11790 at 2502.220 kb on - strand, within IAI47_11790 at 2502.220 kb on - strand, within IAI47_11790 at 2502.220 kb on - strand, within IAI47_11790 at 2502.220 kb on - strand, within IAI47_11790 at 2502.220 kb on - strand, within IAI47_11790 at 2502.220 kb on - strand, within IAI47_11790 at 2502.336 kb on + strand at 2502.336 kb on + strand at 2502.337 kb on - strand at 2502.337 kb on - strand at 2502.337 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 2,498,944 + IAI47_11780 0.33 -1.2 2,498,945 - IAI47_11780 0.34 +0.8 2,498,945 - IAI47_11780 0.34 +0.6 2,499,077 + IAI47_11780 0.43 -1.2 2,499,078 - IAI47_11780 0.43 -0.4 2,499,083 + IAI47_11780 0.43 +0.9 2,499,084 - IAI47_11780 0.43 +0.4 2,499,120 + IAI47_11780 0.46 -0.8 2,499,120 + IAI47_11780 0.46 +1.1 2,499,120 + IAI47_11780 0.46 +1.3 2,499,120 + IAI47_11780 0.46 +0.1 2,499,121 - IAI47_11780 0.46 -1.6 2,499,121 - IAI47_11780 0.46 -0.1 2,499,121 - IAI47_11780 0.46 +0.1 2,499,121 - IAI47_11780 0.46 +0.4 2,499,121 - IAI47_11780 0.46 -0.9 2,499,210 - IAI47_11780 0.52 -0.4 2,499,302 + IAI47_11780 0.59 -0.3 2,499,302 + IAI47_11780 0.59 +0.7 2,499,303 - IAI47_11780 0.59 -1.4 2,499,304 + IAI47_11780 0.59 -1.2 2,499,304 + IAI47_11780 0.59 -1.3 2,499,305 - IAI47_11780 0.59 +1.5 2,499,305 - IAI47_11780 0.59 -2.2 2,499,305 - IAI47_11780 0.59 +1.7 2,499,305 - IAI47_11780 0.59 +1.6 2,499,305 - IAI47_11780 0.59 +0.5 2,499,305 - IAI47_11780 0.59 -0.8 2,499,317 + IAI47_11780 0.60 -1.3 2,499,317 + IAI47_11780 0.60 +1.6 2,499,318 - IAI47_11780 0.60 -1.6 2,499,393 - IAI47_11780 0.65 +0.4 2,499,467 + IAI47_11780 0.70 -0.3 2,499,467 + IAI47_11780 0.70 +0.7 2,499,467 + IAI47_11780 0.70 +0.9 2,499,467 + IAI47_11780 0.70 +1.0 2,499,468 - IAI47_11780 0.70 -0.8 2,499,468 - IAI47_11780 0.70 +0.7 2,499,472 + IAI47_11780 0.71 +0.5 2,499,472 + IAI47_11780 0.71 +1.0 2,499,472 + IAI47_11780 0.71 +1.1 2,499,473 - IAI47_11780 0.71 +1.4 2,499,473 - IAI47_11780 0.71 +1.8 2,499,473 - IAI47_11780 0.71 -0.1 2,499,542 + IAI47_11780 0.76 -0.6 2,499,542 + IAI47_11780 0.76 -1.2 2,499,543 - IAI47_11780 0.76 +1.2 2,499,543 - IAI47_11780 0.76 -0.3 2,499,551 + IAI47_11780 0.76 -0.8 2,499,551 + IAI47_11780 0.76 +0.2 2,499,552 - IAI47_11780 0.76 +2.1 2,499,552 - IAI47_11780 0.76 -0.7 2,499,552 - IAI47_11780 0.76 +0.6 2,499,635 + IAI47_11780 0.82 -0.0 2,499,635 + IAI47_11780 0.82 +0.2 2,499,636 - IAI47_11780 0.82 -0.4 2,499,721 - IAI47_11780 0.88 -1.0 2,499,761 + +0.9 2,499,820 + +0.8 2,499,820 + -1.0 2,499,820 + +0.5 2,499,821 - +0.5 2,499,821 - +0.9 2,499,864 + +0.1 2,499,865 - -0.9 2,499,909 + -0.5 2,499,909 + +0.4 2,499,910 - -0.8 2,499,910 - -0.9 2,499,910 - +0.1 2,499,955 + -2.2 2,499,956 - -0.3 2,499,956 - +1.0 2,499,957 + -0.4 2,499,957 + -0.4 2,499,970 + +1.0 2,499,971 - +1.1 2,499,971 - -0.7 2,500,016 + +3.0 2,500,027 + +0.5 2,500,027 + -1.2 2,500,027 + -0.2 2,500,028 - +0.2 2,500,028 - -1.5 2,500,028 - -0.6 2,500,142 + IAI47_11785 0.16 +0.3 2,500,142 + IAI47_11785 0.16 +1.0 2,500,159 + IAI47_11785 0.17 +0.3 2,500,159 + IAI47_11785 0.17 +1.3 2,500,377 + IAI47_11785 0.32 -1.5 2,500,377 + IAI47_11785 0.32 -0.3 2,500,378 - IAI47_11785 0.32 -0.8 2,500,378 - IAI47_11785 0.32 -1.9 2,500,378 - IAI47_11785 0.32 +1.3 2,500,450 + IAI47_11785 0.36 +1.2 2,500,450 + IAI47_11785 0.36 -0.7 2,500,504 - IAI47_11785 0.40 +0.2 2,500,602 + IAI47_11785 0.47 +2.1 2,500,602 + IAI47_11785 0.47 -0.9 2,500,602 + IAI47_11785 0.47 -2.2 2,500,602 + IAI47_11785 0.47 -0.3 2,500,602 + IAI47_11785 0.47 +0.5 2,500,603 - IAI47_11785 0.47 +0.8 2,500,603 - IAI47_11785 0.47 -0.6 2,500,603 - IAI47_11785 0.47 -2.7 2,500,603 - IAI47_11785 0.47 +0.6 2,500,603 - IAI47_11785 0.47 -2.3 2,500,603 - IAI47_11785 0.47 -0.4 2,500,603 - IAI47_11785 0.47 -1.0 2,500,603 - IAI47_11785 0.47 -1.4 2,500,603 - IAI47_11785 0.47 -2.3 2,500,603 - IAI47_11785 0.47 +1.1 2,500,603 - IAI47_11785 0.47 +0.9 2,500,603 - IAI47_11785 0.47 -1.2 2,500,670 - IAI47_11785 0.51 -1.1 2,500,687 + IAI47_11785 0.52 +1.0 2,500,687 + IAI47_11785 0.52 +1.5 2,500,687 + IAI47_11785 0.52 -0.9 2,500,688 - IAI47_11785 0.52 +0.8 2,500,688 - IAI47_11785 0.52 -1.2 2,500,715 + IAI47_11785 0.54 +0.1 2,500,715 + IAI47_11785 0.54 +1.1 2,500,716 - IAI47_11785 0.54 -1.4 2,500,827 + IAI47_11785 0.62 -0.5 2,500,827 + IAI47_11785 0.62 -0.1 2,500,827 + IAI47_11785 0.62 -0.8 2,500,827 + IAI47_11785 0.62 -0.2 2,500,827 + IAI47_11785 0.62 +1.1 2,500,827 + IAI47_11785 0.62 +1.4 2,500,828 - IAI47_11785 0.62 +0.8 2,500,828 - IAI47_11785 0.62 -1.4 2,500,978 + IAI47_11785 0.72 -0.8 2,501,161 + IAI47_11785 0.84 -1.0 2,501,161 + IAI47_11785 0.84 -2.4 2,501,161 + IAI47_11785 0.84 -0.4 2,501,161 + IAI47_11785 0.84 -1.2 2,501,162 - IAI47_11785 0.84 -0.1 2,501,162 - IAI47_11785 0.84 -0.0 2,501,162 - IAI47_11785 0.84 +0.6 2,501,228 + IAI47_11785 0.89 -0.8 2,501,228 + IAI47_11785 0.89 -1.1 2,501,229 - IAI47_11785 0.89 -0.4 2,501,292 - -1.6 2,501,399 + -0.3 2,501,399 + -0.6 2,501,399 + +0.4 2,501,400 - +0.8 2,501,400 - +0.7 2,501,400 - -0.3 2,501,496 - IAI47_11790 0.10 -0.0 2,501,496 - IAI47_11790 0.10 +0.1 2,501,607 + IAI47_11790 0.22 +1.2 2,501,652 + IAI47_11790 0.27 -2.2 2,501,816 + IAI47_11790 0.44 -1.1 2,501,862 + IAI47_11790 0.49 -1.1 2,501,864 + IAI47_11790 0.49 -0.0 2,501,864 + IAI47_11790 0.49 +0.2 2,501,864 + IAI47_11790 0.49 -0.1 2,501,864 + IAI47_11790 0.49 +1.3 2,501,864 + IAI47_11790 0.49 -0.3 2,501,864 + IAI47_11790 0.49 +0.1 2,501,864 + IAI47_11790 0.49 +0.8 2,501,865 - IAI47_11790 0.49 +0.1 2,501,865 - IAI47_11790 0.49 +0.6 2,501,865 - IAI47_11790 0.49 +0.4 2,501,865 - IAI47_11790 0.49 +1.9 2,501,865 - IAI47_11790 0.49 -1.2 2,501,891 + IAI47_11790 0.52 -1.7 2,501,892 - IAI47_11790 0.52 +0.7 2,501,892 - IAI47_11790 0.52 -0.5 2,501,892 - IAI47_11790 0.52 +1.9 2,501,933 + IAI47_11790 0.57 -1.6 2,501,933 + IAI47_11790 0.57 +0.3 2,501,933 + IAI47_11790 0.57 -1.0 2,501,933 + IAI47_11790 0.57 +1.5 2,501,933 + IAI47_11790 0.57 +0.5 2,501,933 + IAI47_11790 0.57 -2.6 2,501,933 + IAI47_11790 0.57 -1.2 2,501,933 + IAI47_11790 0.57 +0.2 2,501,933 + IAI47_11790 0.57 -1.4 2,501,934 - IAI47_11790 0.57 -1.9 2,501,934 - IAI47_11790 0.57 +1.3 2,501,934 - IAI47_11790 0.57 -0.7 2,501,934 - IAI47_11790 0.57 +0.0 2,502,008 + IAI47_11790 0.65 -0.1 2,502,009 - IAI47_11790 0.65 +0.8 2,502,056 + IAI47_11790 0.70 +0.0 2,502,056 + IAI47_11790 0.70 +1.3 2,502,057 - IAI47_11790 0.70 +1.2 2,502,057 - IAI47_11790 0.70 +0.0 2,502,115 - IAI47_11790 0.76 +0.5 2,502,192 + IAI47_11790 0.84 -0.4 2,502,192 + IAI47_11790 0.84 -0.1 2,502,192 + IAI47_11790 0.84 +0.6 2,502,193 - IAI47_11790 0.84 +2.2 2,502,193 - IAI47_11790 0.84 -1.4 2,502,193 - IAI47_11790 0.84 -1.0 2,502,216 + IAI47_11790 0.87 -0.4 2,502,216 + IAI47_11790 0.87 -1.0 2,502,219 + IAI47_11790 0.87 -2.2 2,502,219 + IAI47_11790 0.87 +2.0 2,502,220 - IAI47_11790 0.87 -1.4 2,502,220 - IAI47_11790 0.87 +0.8 2,502,220 - IAI47_11790 0.87 +1.1 2,502,220 - IAI47_11790 0.87 -1.2 2,502,220 - IAI47_11790 0.87 +0.1 2,502,220 - IAI47_11790 0.87 +0.5 2,502,336 + -0.1 2,502,336 + +0.6 2,502,337 - +1.8 2,502,337 - -0.2 2,502,337 - -1.2
Or see this region's nucleotide sequence