Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_07600 and IAI47_07605 are separated by 40 nucleotides IAI47_07605 and IAI47_07610 overlap by 4 nucleotides IAI47_07610 and IAI47_07615 overlap by 4 nucleotides
IAI47_07600: IAI47_07600 - ABC transporter substrate-binding protein, at 1,608,320 to 1,609,927
_07600
IAI47_07605: IAI47_07605 - ABC transporter permease, at 1,609,968 to 1,610,924
_07605
IAI47_07610: IAI47_07610 - ABC transporter permease, at 1,610,921 to 1,611,772
_07610
IAI47_07615: IAI47_07615 - ABC transporter ATP-binding protein, at 1,611,769 to 1,613,433
_07615
Position (kb)
1610
1611
1612 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1609.954 kb on + strand at 1609.955 kb on - strand at 1609.961 kb on + strand at 1609.961 kb on + strand at 1609.961 kb on + strand at 1609.961 kb on + strand at 1609.977 kb on + strand at 1609.977 kb on + strand at 1609.978 kb on - strand at 1609.978 kb on - strand at 1610.030 kb on + strand at 1610.172 kb on + strand, within IAI47_07605 at 1610.172 kb on + strand, within IAI47_07605 at 1610.172 kb on + strand, within IAI47_07605 at 1610.173 kb on - strand, within IAI47_07605 at 1610.173 kb on - strand, within IAI47_07605 at 1610.174 kb on + strand, within IAI47_07605 at 1610.174 kb on + strand, within IAI47_07605 at 1610.174 kb on + strand, within IAI47_07605 at 1610.175 kb on - strand, within IAI47_07605 at 1610.175 kb on - strand, within IAI47_07605 at 1610.262 kb on - strand, within IAI47_07605 at 1610.508 kb on + strand, within IAI47_07605 at 1610.508 kb on + strand, within IAI47_07605 at 1610.509 kb on - strand, within IAI47_07605 at 1610.520 kb on + strand, within IAI47_07605 at 1610.520 kb on + strand, within IAI47_07605 at 1610.521 kb on - strand, within IAI47_07605 at 1610.521 kb on - strand, within IAI47_07605 at 1610.591 kb on + strand, within IAI47_07605 at 1610.592 kb on - strand, within IAI47_07605 at 1610.613 kb on + strand, within IAI47_07605 at 1610.614 kb on - strand, within IAI47_07605 at 1610.614 kb on - strand, within IAI47_07605 at 1610.614 kb on - strand, within IAI47_07605 at 1610.614 kb on - strand, within IAI47_07605 at 1610.614 kb on - strand, within IAI47_07605 at 1610.724 kb on + strand, within IAI47_07605 at 1610.724 kb on + strand, within IAI47_07605 at 1610.724 kb on + strand, within IAI47_07605 at 1610.724 kb on + strand, within IAI47_07605 at 1610.725 kb on - strand, within IAI47_07605 at 1610.725 kb on - strand, within IAI47_07605 at 1610.725 kb on - strand, within IAI47_07605 at 1610.766 kb on + strand, within IAI47_07605 at 1610.767 kb on - strand, within IAI47_07605 at 1610.856 kb on + strand at 1610.856 kb on + strand at 1610.856 kb on + strand at 1610.856 kb on + strand at 1610.857 kb on - strand at 1610.857 kb on - strand at 1610.857 kb on - strand at 1610.900 kb on + strand at 1610.900 kb on + strand at 1610.926 kb on + strand at 1610.926 kb on + strand at 1610.927 kb on - strand at 1610.927 kb on - strand at 1611.019 kb on - strand, within IAI47_07610 at 1611.113 kb on + strand, within IAI47_07610 at 1611.113 kb on + strand, within IAI47_07610 at 1611.114 kb on - strand, within IAI47_07610 at 1611.140 kb on - strand, within IAI47_07610 at 1611.258 kb on + strand, within IAI47_07610 at 1611.375 kb on - strand, within IAI47_07610 at 1611.375 kb on - strand, within IAI47_07610 at 1611.424 kb on + strand, within IAI47_07610 at 1611.424 kb on + strand, within IAI47_07610 at 1611.424 kb on + strand, within IAI47_07610 at 1611.425 kb on - strand, within IAI47_07610 at 1611.425 kb on - strand, within IAI47_07610 at 1611.434 kb on + strand, within IAI47_07610 at 1611.434 kb on + strand, within IAI47_07610 at 1611.434 kb on + strand, within IAI47_07610 at 1611.435 kb on - strand, within IAI47_07610 at 1611.435 kb on - strand, within IAI47_07610 at 1611.435 kb on - strand, within IAI47_07610 at 1611.435 kb on - strand, within IAI47_07610 at 1611.473 kb on + strand, within IAI47_07610 at 1611.473 kb on + strand, within IAI47_07610 at 1611.473 kb on + strand, within IAI47_07610 at 1611.473 kb on + strand, within IAI47_07610 at 1611.473 kb on + strand, within IAI47_07610 at 1611.473 kb on + strand, within IAI47_07610 at 1611.474 kb on - strand, within IAI47_07610 at 1611.474 kb on - strand, within IAI47_07610 at 1611.474 kb on - strand, within IAI47_07610 at 1611.474 kb on - strand, within IAI47_07610 at 1611.475 kb on + strand, within IAI47_07610 at 1611.475 kb on + strand, within IAI47_07610 at 1611.475 kb on + strand, within IAI47_07610 at 1611.476 kb on - strand, within IAI47_07610 at 1611.500 kb on - strand, within IAI47_07610 at 1611.500 kb on - strand, within IAI47_07610 at 1611.545 kb on + strand, within IAI47_07610 at 1611.545 kb on + strand, within IAI47_07610 at 1611.545 kb on + strand, within IAI47_07610 at 1611.546 kb on - strand, within IAI47_07610 at 1611.546 kb on - strand, within IAI47_07610 at 1611.546 kb on - strand, within IAI47_07610 at 1611.546 kb on - strand, within IAI47_07610 at 1611.547 kb on + strand, within IAI47_07610 at 1611.602 kb on + strand, within IAI47_07610 at 1611.603 kb on - strand, within IAI47_07610 at 1611.603 kb on - strand, within IAI47_07610 at 1611.634 kb on + strand, within IAI47_07610 at 1611.634 kb on + strand, within IAI47_07610 at 1611.635 kb on - strand, within IAI47_07610 at 1611.645 kb on + strand, within IAI47_07610 at 1611.645 kb on + strand, within IAI47_07610 at 1611.786 kb on + strand at 1611.786 kb on + strand at 1611.787 kb on - strand at 1611.787 kb on - strand at 1611.787 kb on - strand at 1611.787 kb on - strand at 1611.787 kb on - strand at 1611.804 kb on - strand at 1611.804 kb on - strand at 1611.880 kb on - strand at 1611.911 kb on + strand at 1611.930 kb on + strand at 1611.930 kb on + strand at 1611.931 kb on - strand at 1611.961 kb on + strand, within IAI47_07615 at 1611.961 kb on + strand, within IAI47_07615 at 1611.962 kb on - strand, within IAI47_07615 at 1611.962 kb on - strand, within IAI47_07615 at 1611.962 kb on - strand, within IAI47_07615 at 1612.020 kb on + strand, within IAI47_07615 at 1612.021 kb on - strand, within IAI47_07615 at 1612.021 kb on - strand, within IAI47_07615 at 1612.043 kb on + strand, within IAI47_07615 at 1612.043 kb on + strand, within IAI47_07615 at 1612.043 kb on + strand, within IAI47_07615 at 1612.044 kb on - strand, within IAI47_07615 at 1612.072 kb on + strand, within IAI47_07615 at 1612.072 kb on + strand, within IAI47_07615 at 1612.072 kb on + strand, within IAI47_07615 at 1612.073 kb on - strand, within IAI47_07615 at 1612.075 kb on - strand at 1612.102 kb on - strand, within IAI47_07615 at 1612.142 kb on + strand, within IAI47_07615 at 1612.142 kb on + strand, within IAI47_07615 at 1612.146 kb on + strand, within IAI47_07615 at 1612.146 kb on + strand, within IAI47_07615 at 1612.147 kb on - strand, within IAI47_07615 at 1612.147 kb on - strand, within IAI47_07615 at 1612.190 kb on + strand, within IAI47_07615 at 1612.190 kb on + strand, within IAI47_07615 at 1612.190 kb on + strand, within IAI47_07615 at 1612.191 kb on - strand, within IAI47_07615 at 1612.191 kb on - strand, within IAI47_07615 at 1612.232 kb on + strand, within IAI47_07615 at 1612.256 kb on + strand, within IAI47_07615 at 1612.271 kb on + strand, within IAI47_07615 at 1612.271 kb on + strand, within IAI47_07615 at 1612.272 kb on - strand, within IAI47_07615 at 1612.272 kb on - strand, within IAI47_07615 at 1612.272 kb on - strand, within IAI47_07615 at 1612.272 kb on - strand, within IAI47_07615 at 1612.272 kb on - strand, within IAI47_07615 at 1612.272 kb on - strand, within IAI47_07615 at 1612.272 kb on - strand, within IAI47_07615 at 1612.272 kb on - strand, within IAI47_07615 at 1612.272 kb on - strand, within IAI47_07615 at 1612.272 kb on - strand, within IAI47_07615 at 1612.420 kb on + strand, within IAI47_07615 at 1612.420 kb on + strand, within IAI47_07615 at 1612.420 kb on + strand, within IAI47_07615 at 1612.420 kb on + strand, within IAI47_07615 at 1612.421 kb on - strand, within IAI47_07615 at 1612.421 kb on - strand, within IAI47_07615 at 1612.498 kb on + strand, within IAI47_07615 at 1612.498 kb on + strand, within IAI47_07615 at 1612.499 kb on - strand, within IAI47_07615 at 1612.499 kb on - strand, within IAI47_07615 at 1612.540 kb on + strand, within IAI47_07615 at 1612.541 kb on - strand, within IAI47_07615 at 1612.550 kb on + strand, within IAI47_07615 at 1612.550 kb on + strand, within IAI47_07615 at 1612.551 kb on - strand, within IAI47_07615 at 1612.740 kb on + strand, within IAI47_07615
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 1,609,954 + -0.7 1,609,955 - +1.9 1,609,961 + -0.3 1,609,961 + -1.3 1,609,961 + +0.1 1,609,961 + -3.4 1,609,977 + +0.1 1,609,977 + -0.3 1,609,978 - +0.3 1,609,978 - -1.6 1,610,030 + +1.4 1,610,172 + IAI47_07605 0.21 +1.0 1,610,172 + IAI47_07605 0.21 +0.5 1,610,172 + IAI47_07605 0.21 +0.5 1,610,173 - IAI47_07605 0.21 -1.1 1,610,173 - IAI47_07605 0.21 +0.6 1,610,174 + IAI47_07605 0.22 -0.0 1,610,174 + IAI47_07605 0.22 +1.4 1,610,174 + IAI47_07605 0.22 +0.1 1,610,175 - IAI47_07605 0.22 -1.7 1,610,175 - IAI47_07605 0.22 -0.2 1,610,262 - IAI47_07605 0.31 +1.2 1,610,508 + IAI47_07605 0.56 +0.0 1,610,508 + IAI47_07605 0.56 +2.4 1,610,509 - IAI47_07605 0.57 +1.7 1,610,520 + IAI47_07605 0.58 -1.0 1,610,520 + IAI47_07605 0.58 +0.6 1,610,521 - IAI47_07605 0.58 +2.2 1,610,521 - IAI47_07605 0.58 -1.4 1,610,591 + IAI47_07605 0.65 +0.4 1,610,592 - IAI47_07605 0.65 +1.2 1,610,613 + IAI47_07605 0.67 -0.0 1,610,614 - IAI47_07605 0.68 +0.5 1,610,614 - IAI47_07605 0.68 +1.8 1,610,614 - IAI47_07605 0.68 +0.4 1,610,614 - IAI47_07605 0.68 +2.2 1,610,614 - IAI47_07605 0.68 +0.7 1,610,724 + IAI47_07605 0.79 +0.6 1,610,724 + IAI47_07605 0.79 +0.1 1,610,724 + IAI47_07605 0.79 +0.1 1,610,724 + IAI47_07605 0.79 +0.6 1,610,725 - IAI47_07605 0.79 +1.8 1,610,725 - IAI47_07605 0.79 +1.9 1,610,725 - IAI47_07605 0.79 +1.8 1,610,766 + IAI47_07605 0.83 +2.5 1,610,767 - IAI47_07605 0.83 -0.8 1,610,856 + +0.1 1,610,856 + +1.7 1,610,856 + +0.6 1,610,856 + -1.3 1,610,857 - -1.1 1,610,857 - -2.2 1,610,857 - -1.9 1,610,900 + -0.1 1,610,900 + +0.0 1,610,926 + +1.1 1,610,926 + -1.4 1,610,927 - +0.3 1,610,927 - +1.6 1,611,019 - IAI47_07610 0.12 -0.7 1,611,113 + IAI47_07610 0.23 -0.9 1,611,113 + IAI47_07610 0.23 -1.6 1,611,114 - IAI47_07610 0.23 +0.5 1,611,140 - IAI47_07610 0.26 +0.5 1,611,258 + IAI47_07610 0.40 +2.2 1,611,375 - IAI47_07610 0.53 -0.5 1,611,375 - IAI47_07610 0.53 +0.3 1,611,424 + IAI47_07610 0.59 -1.1 1,611,424 + IAI47_07610 0.59 -2.0 1,611,424 + IAI47_07610 0.59 -0.7 1,611,425 - IAI47_07610 0.59 -0.4 1,611,425 - IAI47_07610 0.59 -0.3 1,611,434 + IAI47_07610 0.60 +1.3 1,611,434 + IAI47_07610 0.60 -0.7 1,611,434 + IAI47_07610 0.60 +0.3 1,611,435 - IAI47_07610 0.60 +0.7 1,611,435 - IAI47_07610 0.60 -0.3 1,611,435 - IAI47_07610 0.60 -0.5 1,611,435 - IAI47_07610 0.60 +1.5 1,611,473 + IAI47_07610 0.65 +2.2 1,611,473 + IAI47_07610 0.65 +0.7 1,611,473 + IAI47_07610 0.65 -1.9 1,611,473 + IAI47_07610 0.65 -0.1 1,611,473 + IAI47_07610 0.65 -2.6 1,611,473 + IAI47_07610 0.65 +0.8 1,611,474 - IAI47_07610 0.65 -0.7 1,611,474 - IAI47_07610 0.65 -0.3 1,611,474 - IAI47_07610 0.65 -1.2 1,611,474 - IAI47_07610 0.65 +0.5 1,611,475 + IAI47_07610 0.65 +0.0 1,611,475 + IAI47_07610 0.65 +0.4 1,611,475 + IAI47_07610 0.65 -0.1 1,611,476 - IAI47_07610 0.65 -2.2 1,611,500 - IAI47_07610 0.68 -1.5 1,611,500 - IAI47_07610 0.68 +0.6 1,611,545 + IAI47_07610 0.73 -1.1 1,611,545 + IAI47_07610 0.73 -0.9 1,611,545 + IAI47_07610 0.73 -2.2 1,611,546 - IAI47_07610 0.73 -1.1 1,611,546 - IAI47_07610 0.73 -0.6 1,611,546 - IAI47_07610 0.73 +0.3 1,611,546 - IAI47_07610 0.73 +1.4 1,611,547 + IAI47_07610 0.73 -0.0 1,611,602 + IAI47_07610 0.80 -0.2 1,611,603 - IAI47_07610 0.80 +0.1 1,611,603 - IAI47_07610 0.80 -0.8 1,611,634 + IAI47_07610 0.84 -3.2 1,611,634 + IAI47_07610 0.84 -1.7 1,611,635 - IAI47_07610 0.84 -0.4 1,611,645 + IAI47_07610 0.85 +0.3 1,611,645 + IAI47_07610 0.85 +0.7 1,611,786 + -1.2 1,611,786 + +0.6 1,611,787 - -0.7 1,611,787 - -1.7 1,611,787 - +0.4 1,611,787 - -0.6 1,611,787 - -2.2 1,611,804 - +0.2 1,611,804 - +0.0 1,611,880 - -0.1 1,611,911 + +0.3 1,611,930 + -2.1 1,611,930 + +0.6 1,611,931 - +0.5 1,611,961 + IAI47_07615 0.12 +0.5 1,611,961 + IAI47_07615 0.12 +0.3 1,611,962 - IAI47_07615 0.12 +0.7 1,611,962 - IAI47_07615 0.12 +0.3 1,611,962 - IAI47_07615 0.12 +1.2 1,612,020 + IAI47_07615 0.15 -0.1 1,612,021 - IAI47_07615 0.15 +0.4 1,612,021 - IAI47_07615 0.15 -2.0 1,612,043 + IAI47_07615 0.16 +1.2 1,612,043 + IAI47_07615 0.16 -1.8 1,612,043 + IAI47_07615 0.16 +1.1 1,612,044 - IAI47_07615 0.17 -0.8 1,612,072 + IAI47_07615 0.18 +0.4 1,612,072 + IAI47_07615 0.18 +0.5 1,612,072 + IAI47_07615 0.18 +1.2 1,612,073 - IAI47_07615 0.18 +0.5 1,612,075 - -1.3 1,612,102 - IAI47_07615 0.20 +1.4 1,612,142 + IAI47_07615 0.22 +2.7 1,612,142 + IAI47_07615 0.22 +1.0 1,612,146 + IAI47_07615 0.23 +1.0 1,612,146 + IAI47_07615 0.23 -0.4 1,612,147 - IAI47_07615 0.23 +0.2 1,612,147 - IAI47_07615 0.23 -0.2 1,612,190 + IAI47_07615 0.25 +0.2 1,612,190 + IAI47_07615 0.25 +0.3 1,612,190 + IAI47_07615 0.25 +0.6 1,612,191 - IAI47_07615 0.25 +1.8 1,612,191 - IAI47_07615 0.25 -1.3 1,612,232 + IAI47_07615 0.28 -1.0 1,612,256 + IAI47_07615 0.29 +1.3 1,612,271 + IAI47_07615 0.30 -0.7 1,612,271 + IAI47_07615 0.30 +0.7 1,612,272 - IAI47_07615 0.30 -0.1 1,612,272 - IAI47_07615 0.30 +0.8 1,612,272 - IAI47_07615 0.30 -1.7 1,612,272 - IAI47_07615 0.30 +0.6 1,612,272 - IAI47_07615 0.30 -0.5 1,612,272 - IAI47_07615 0.30 -1.8 1,612,272 - IAI47_07615 0.30 +1.5 1,612,272 - IAI47_07615 0.30 +1.3 1,612,272 - IAI47_07615 0.30 +1.3 1,612,272 - IAI47_07615 0.30 +2.1 1,612,420 + IAI47_07615 0.39 -1.5 1,612,420 + IAI47_07615 0.39 -0.8 1,612,420 + IAI47_07615 0.39 +0.7 1,612,420 + IAI47_07615 0.39 +1.7 1,612,421 - IAI47_07615 0.39 +0.0 1,612,421 - IAI47_07615 0.39 +0.0 1,612,498 + IAI47_07615 0.44 -2.7 1,612,498 + IAI47_07615 0.44 +0.8 1,612,499 - IAI47_07615 0.44 +0.0 1,612,499 - IAI47_07615 0.44 -0.3 1,612,540 + IAI47_07615 0.46 -1.6 1,612,541 - IAI47_07615 0.46 -0.7 1,612,550 + IAI47_07615 0.47 +0.0 1,612,550 + IAI47_07615 0.47 +0.8 1,612,551 - IAI47_07615 0.47 +0.1 1,612,740 + IAI47_07615 0.58 +1.7
Or see this region's nucleotide sequence