Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_04095 and IAI47_04100 are separated by 19 nucleotides IAI47_04100 and IAI47_04105 are separated by 74 nucleotides IAI47_04105 and IAI47_04110 are separated by 188 nucleotides
IAI47_04095: IAI47_04095 - 2-dehydro-3-deoxyglucarate aldolase, at 856,687 to 857,457
_04095
IAI47_04100: IAI47_04100 - 2-hydroxy-3-oxopropionate reductase, at 857,477 to 858,361
_04100
IAI47_04105: IAI47_04105 - glycerate kinase, at 858,436 to 859,575
_04105
IAI47_04110: IAI47_04110 - two-component sensor histidine kinase BarA, at 859,764 to 862,493
_04110
Position (kb) 858
859
860 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 857.449 kb on - strand at 857.449 kb on - strand at 857.449 kb on - strand at 857.463 kb on + strand at 857.464 kb on - strand at 857.491 kb on + strand at 857.556 kb on + strand at 857.557 kb on - strand at 857.557 kb on - strand at 857.596 kb on + strand, within IAI47_04100 at 857.597 kb on - strand, within IAI47_04100 at 857.659 kb on + strand, within IAI47_04100 at 857.659 kb on + strand, within IAI47_04100 at 857.659 kb on + strand, within IAI47_04100 at 857.659 kb on + strand, within IAI47_04100 at 857.660 kb on - strand, within IAI47_04100 at 857.660 kb on - strand, within IAI47_04100 at 857.660 kb on - strand, within IAI47_04100 at 857.660 kb on - strand, within IAI47_04100 at 857.717 kb on - strand, within IAI47_04100 at 857.787 kb on + strand, within IAI47_04100 at 857.788 kb on - strand, within IAI47_04100 at 857.893 kb on + strand, within IAI47_04100 at 857.894 kb on - strand, within IAI47_04100 at 857.915 kb on + strand, within IAI47_04100 at 857.915 kb on + strand, within IAI47_04100 at 857.915 kb on + strand, within IAI47_04100 at 857.915 kb on + strand, within IAI47_04100 at 857.915 kb on + strand, within IAI47_04100 at 857.916 kb on - strand, within IAI47_04100 at 857.916 kb on - strand, within IAI47_04100 at 857.954 kb on - strand, within IAI47_04100 at 857.983 kb on + strand, within IAI47_04100 at 857.983 kb on + strand, within IAI47_04100 at 857.984 kb on - strand, within IAI47_04100 at 857.984 kb on - strand, within IAI47_04100 at 858.081 kb on - strand, within IAI47_04100 at 858.211 kb on + strand, within IAI47_04100 at 858.211 kb on + strand, within IAI47_04100 at 858.212 kb on - strand, within IAI47_04100 at 858.212 kb on - strand, within IAI47_04100 at 858.323 kb on + strand at 858.323 kb on + strand at 858.323 kb on + strand at 858.324 kb on - strand at 858.324 kb on - strand at 858.324 kb on - strand at 858.326 kb on + strand at 858.326 kb on + strand at 858.326 kb on + strand at 858.326 kb on + strand at 858.327 kb on - strand at 858.327 kb on - strand at 858.337 kb on - strand at 858.337 kb on - strand at 858.359 kb on + strand at 858.463 kb on + strand at 858.463 kb on + strand at 858.463 kb on + strand at 858.463 kb on + strand at 858.463 kb on + strand at 858.464 kb on - strand at 858.464 kb on - strand at 858.465 kb on + strand at 858.465 kb on + strand at 858.466 kb on - strand at 858.466 kb on - strand at 858.466 kb on - strand at 858.466 kb on - strand at 858.476 kb on + strand at 858.541 kb on + strand at 858.541 kb on + strand at 858.541 kb on + strand at 858.541 kb on + strand at 858.542 kb on - strand at 858.542 kb on - strand at 858.542 kb on - strand at 858.542 kb on - strand at 858.581 kb on + strand, within IAI47_04105 at 858.581 kb on + strand, within IAI47_04105 at 858.581 kb on + strand, within IAI47_04105 at 858.581 kb on + strand, within IAI47_04105 at 858.582 kb on - strand, within IAI47_04105 at 858.582 kb on - strand, within IAI47_04105 at 858.664 kb on + strand, within IAI47_04105 at 858.664 kb on + strand, within IAI47_04105 at 858.665 kb on - strand, within IAI47_04105 at 858.665 kb on - strand, within IAI47_04105 at 858.694 kb on + strand, within IAI47_04105 at 858.694 kb on + strand, within IAI47_04105 at 858.694 kb on + strand, within IAI47_04105 at 858.694 kb on + strand, within IAI47_04105 at 858.694 kb on + strand, within IAI47_04105 at 858.694 kb on + strand, within IAI47_04105 at 858.694 kb on + strand, within IAI47_04105 at 858.695 kb on - strand, within IAI47_04105 at 858.695 kb on - strand, within IAI47_04105 at 858.695 kb on - strand, within IAI47_04105 at 858.696 kb on + strand, within IAI47_04105 at 858.758 kb on + strand, within IAI47_04105 at 858.819 kb on + strand, within IAI47_04105 at 858.820 kb on - strand, within IAI47_04105 at 858.820 kb on - strand, within IAI47_04105 at 858.832 kb on - strand, within IAI47_04105 at 858.960 kb on + strand, within IAI47_04105 at 858.961 kb on - strand, within IAI47_04105 at 858.961 kb on - strand, within IAI47_04105 at 859.021 kb on - strand, within IAI47_04105 at 859.121 kb on - strand, within IAI47_04105 at 859.121 kb on - strand, within IAI47_04105 at 859.121 kb on - strand, within IAI47_04105 at 859.121 kb on - strand, within IAI47_04105 at 859.131 kb on + strand, within IAI47_04105 at 859.132 kb on - strand, within IAI47_04105 at 859.149 kb on + strand, within IAI47_04105 at 859.150 kb on - strand, within IAI47_04105 at 859.303 kb on - strand, within IAI47_04105 at 859.332 kb on + strand, within IAI47_04105 at 859.332 kb on + strand, within IAI47_04105 at 859.332 kb on + strand, within IAI47_04105 at 859.332 kb on + strand, within IAI47_04105 at 859.333 kb on - strand, within IAI47_04105 at 859.333 kb on - strand, within IAI47_04105 at 859.333 kb on - strand, within IAI47_04105 at 859.333 kb on - strand, within IAI47_04105 at 859.333 kb on - strand, within IAI47_04105 at 859.335 kb on + strand, within IAI47_04105 at 859.335 kb on + strand, within IAI47_04105 at 859.336 kb on - strand, within IAI47_04105 at 859.381 kb on - strand, within IAI47_04105 at 859.381 kb on - strand, within IAI47_04105 at 859.398 kb on + strand, within IAI47_04105 at 859.399 kb on - strand, within IAI47_04105 at 859.399 kb on - strand, within IAI47_04105 at 859.399 kb on - strand, within IAI47_04105 at 859.471 kb on + strand at 859.471 kb on + strand at 859.471 kb on + strand at 859.471 kb on + strand at 859.471 kb on + strand at 859.471 kb on + strand at 859.472 kb on - strand at 859.472 kb on - strand at 859.472 kb on - strand at 859.536 kb on + strand at 859.536 kb on + strand at 859.537 kb on - strand at 859.537 kb on - strand at 859.537 kb on - strand at 859.537 kb on - strand at 859.537 kb on - strand at 859.537 kb on - strand at 859.594 kb on + strand at 859.594 kb on + strand at 859.595 kb on - strand at 859.595 kb on - strand at 859.595 kb on - strand at 859.595 kb on - strand at 859.595 kb on - strand at 859.597 kb on + strand at 859.597 kb on + strand at 859.598 kb on - strand at 859.598 kb on - strand at 859.634 kb on - strand at 859.647 kb on + strand at 859.664 kb on + strand at 859.664 kb on + strand at 859.697 kb on + strand at 859.697 kb on + strand at 859.697 kb on + strand at 859.697 kb on + strand at 859.697 kb on + strand at 859.697 kb on + strand at 859.697 kb on + strand at 859.697 kb on + strand at 859.698 kb on - strand at 859.698 kb on - strand at 859.698 kb on - strand at 859.702 kb on + strand at 859.703 kb on - strand at 859.776 kb on + strand at 859.776 kb on + strand at 859.921 kb on + strand at 859.922 kb on - strand at 859.949 kb on + strand at 859.949 kb on + strand at 860.016 kb on + strand at 860.063 kb on + strand, within IAI47_04110 at 860.063 kb on + strand, within IAI47_04110 at 860.106 kb on + strand, within IAI47_04110 at 860.109 kb on + strand, within IAI47_04110 at 860.109 kb on + strand, within IAI47_04110 at 860.109 kb on + strand, within IAI47_04110 at 860.109 kb on + strand, within IAI47_04110 at 860.109 kb on + strand, within IAI47_04110 at 860.109 kb on + strand, within IAI47_04110 at 860.109 kb on + strand, within IAI47_04110 at 860.110 kb on - strand, within IAI47_04110 at 860.110 kb on - strand, within IAI47_04110 at 860.110 kb on - strand, within IAI47_04110 at 860.110 kb on - strand, within IAI47_04110 at 860.110 kb on - strand, within IAI47_04110 at 860.110 kb on - strand, within IAI47_04110 at 860.110 kb on - strand, within IAI47_04110 at 860.150 kb on + strand, within IAI47_04110 at 860.150 kb on + strand, within IAI47_04110 at 860.150 kb on + strand, within IAI47_04110 at 860.151 kb on - strand, within IAI47_04110 at 860.152 kb on + strand, within IAI47_04110 at 860.152 kb on + strand, within IAI47_04110 at 860.152 kb on + strand, within IAI47_04110 at 860.152 kb on + strand, within IAI47_04110 at 860.153 kb on - strand, within IAI47_04110 at 860.173 kb on - strand, within IAI47_04110 at 860.173 kb on - strand, within IAI47_04110 at 860.203 kb on + strand, within IAI47_04110 at 860.203 kb on + strand, within IAI47_04110 at 860.203 kb on + strand, within IAI47_04110 at 860.203 kb on + strand, within IAI47_04110 at 860.204 kb on - strand, within IAI47_04110 at 860.204 kb on - strand, within IAI47_04110 at 860.204 kb on - strand, within IAI47_04110 at 860.306 kb on + strand, within IAI47_04110 at 860.348 kb on + strand, within IAI47_04110 at 860.348 kb on + strand, within IAI47_04110 at 860.348 kb on + strand, within IAI47_04110 at 860.348 kb on + strand, within IAI47_04110 at 860.393 kb on + strand, within IAI47_04110 at 860.394 kb on - strand, within IAI47_04110 at 860.462 kb on + strand, within IAI47_04110 at 860.463 kb on - strand, within IAI47_04110 at 860.463 kb on - strand, within IAI47_04110 at 860.463 kb on - strand, within IAI47_04110 at 860.463 kb on - strand, within IAI47_04110 at 860.477 kb on + strand, within IAI47_04110 at 860.478 kb on - strand, within IAI47_04110 at 860.478 kb on - strand, within IAI47_04110 at 860.478 kb on - strand, within IAI47_04110 at 860.478 kb on - strand, within IAI47_04110 at 860.557 kb on - strand, within IAI47_04110
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 857,449 - +0.4 857,449 - -0.5 857,449 - +3.4 857,463 + -1.9 857,464 - -1.2 857,491 + +0.4 857,556 + +0.4 857,557 - +0.5 857,557 - -0.4 857,596 + IAI47_04100 0.13 +0.6 857,597 - IAI47_04100 0.14 -0.5 857,659 + IAI47_04100 0.21 -0.1 857,659 + IAI47_04100 0.21 +0.9 857,659 + IAI47_04100 0.21 -0.7 857,659 + IAI47_04100 0.21 -0.3 857,660 - IAI47_04100 0.21 +0.9 857,660 - IAI47_04100 0.21 +3.3 857,660 - IAI47_04100 0.21 +1.0 857,660 - IAI47_04100 0.21 -0.8 857,717 - IAI47_04100 0.27 +0.6 857,787 + IAI47_04100 0.35 +0.8 857,788 - IAI47_04100 0.35 +1.1 857,893 + IAI47_04100 0.47 -2.8 857,894 - IAI47_04100 0.47 +0.9 857,915 + IAI47_04100 0.49 +0.9 857,915 + IAI47_04100 0.49 +1.8 857,915 + IAI47_04100 0.49 +1.3 857,915 + IAI47_04100 0.49 -0.7 857,915 + IAI47_04100 0.49 +1.4 857,916 - IAI47_04100 0.50 +1.8 857,916 - IAI47_04100 0.50 +0.5 857,954 - IAI47_04100 0.54 -0.1 857,983 + IAI47_04100 0.57 -0.7 857,983 + IAI47_04100 0.57 +0.8 857,984 - IAI47_04100 0.57 +1.0 857,984 - IAI47_04100 0.57 -0.8 858,081 - IAI47_04100 0.68 +0.1 858,211 + IAI47_04100 0.83 -0.5 858,211 + IAI47_04100 0.83 -1.0 858,212 - IAI47_04100 0.83 +0.5 858,212 - IAI47_04100 0.83 +3.2 858,323 + -0.7 858,323 + +0.6 858,323 + -1.5 858,324 - +1.0 858,324 - -1.4 858,324 - -0.9 858,326 + +0.1 858,326 + +0.6 858,326 + +1.2 858,326 + -0.3 858,327 - -1.5 858,327 - -1.6 858,337 - -0.1 858,337 - +2.1 858,359 + -0.3 858,463 + -0.5 858,463 + -2.7 858,463 + -1.9 858,463 + -0.2 858,463 + -1.2 858,464 - +1.1 858,464 - -1.9 858,465 + -1.7 858,465 + -0.4 858,466 - -0.3 858,466 - -1.4 858,466 - -1.4 858,466 - -2.0 858,476 + -1.3 858,541 + -0.2 858,541 + -1.6 858,541 + -1.3 858,541 + -1.0 858,542 - -3.0 858,542 - -0.9 858,542 - -0.2 858,542 - +0.3 858,581 + IAI47_04105 0.13 -0.7 858,581 + IAI47_04105 0.13 -1.9 858,581 + IAI47_04105 0.13 -2.5 858,581 + IAI47_04105 0.13 -3.0 858,582 - IAI47_04105 0.13 +0.2 858,582 - IAI47_04105 0.13 -1.2 858,664 + IAI47_04105 0.20 -1.6 858,664 + IAI47_04105 0.20 -0.6 858,665 - IAI47_04105 0.20 -1.1 858,665 - IAI47_04105 0.20 -0.7 858,694 + IAI47_04105 0.23 -0.6 858,694 + IAI47_04105 0.23 -0.8 858,694 + IAI47_04105 0.23 -1.9 858,694 + IAI47_04105 0.23 -1.4 858,694 + IAI47_04105 0.23 -0.4 858,694 + IAI47_04105 0.23 -1.3 858,694 + IAI47_04105 0.23 -1.7 858,695 - IAI47_04105 0.23 -2.3 858,695 - IAI47_04105 0.23 -2.8 858,695 - IAI47_04105 0.23 -1.1 858,696 + IAI47_04105 0.23 -1.4 858,758 + IAI47_04105 0.28 -0.1 858,819 + IAI47_04105 0.34 -1.7 858,820 - IAI47_04105 0.34 -1.7 858,820 - IAI47_04105 0.34 -2.1 858,832 - IAI47_04105 0.35 -1.2 858,960 + IAI47_04105 0.46 -2.3 858,961 - IAI47_04105 0.46 -2.5 858,961 - IAI47_04105 0.46 -1.8 859,021 - IAI47_04105 0.51 -0.5 859,121 - IAI47_04105 0.60 -2.8 859,121 - IAI47_04105 0.60 -1.9 859,121 - IAI47_04105 0.60 -0.7 859,121 - IAI47_04105 0.60 -1.2 859,131 + IAI47_04105 0.61 +1.1 859,132 - IAI47_04105 0.61 +0.5 859,149 + IAI47_04105 0.63 -2.8 859,150 - IAI47_04105 0.63 -1.2 859,303 - IAI47_04105 0.76 -1.4 859,332 + IAI47_04105 0.79 -1.7 859,332 + IAI47_04105 0.79 -1.4 859,332 + IAI47_04105 0.79 +0.1 859,332 + IAI47_04105 0.79 -2.4 859,333 - IAI47_04105 0.79 -0.5 859,333 - IAI47_04105 0.79 -1.3 859,333 - IAI47_04105 0.79 -2.0 859,333 - IAI47_04105 0.79 -3.1 859,333 - IAI47_04105 0.79 +0.1 859,335 + IAI47_04105 0.79 -0.8 859,335 + IAI47_04105 0.79 +1.8 859,336 - IAI47_04105 0.79 -0.2 859,381 - IAI47_04105 0.83 +0.2 859,381 - IAI47_04105 0.83 -2.1 859,398 + IAI47_04105 0.84 -1.9 859,399 - IAI47_04105 0.84 -1.8 859,399 - IAI47_04105 0.84 -0.0 859,399 - IAI47_04105 0.84 -1.7 859,471 + -1.9 859,471 + -1.7 859,471 + -1.6 859,471 + +0.6 859,471 + +0.3 859,471 + +0.3 859,472 - -1.2 859,472 - -3.4 859,472 - +2.0 859,536 + -1.5 859,536 + -2.6 859,537 - -0.7 859,537 - -1.2 859,537 - -3.0 859,537 - -2.0 859,537 - -1.0 859,537 - +0.6 859,594 + -0.6 859,594 + +1.2 859,595 - +0.3 859,595 - +0.1 859,595 - -1.1 859,595 - +1.2 859,595 - -1.4 859,597 + -1.6 859,597 + -0.4 859,598 - +1.4 859,598 - +0.4 859,634 - +0.1 859,647 + -0.6 859,664 + +1.1 859,664 + -2.3 859,697 + +0.6 859,697 + -3.0 859,697 + -0.4 859,697 + -1.0 859,697 + +0.7 859,697 + -2.1 859,697 + +0.2 859,697 + +2.6 859,698 - +0.5 859,698 - +0.8 859,698 - -1.3 859,702 + -0.4 859,703 - -0.9 859,776 + +0.7 859,776 + -0.2 859,921 + -2.7 859,922 - +1.2 859,949 + -0.7 859,949 + -0.2 860,016 + +1.1 860,063 + IAI47_04110 0.11 -1.5 860,063 + IAI47_04110 0.11 -2.1 860,106 + IAI47_04110 0.13 +1.2 860,109 + IAI47_04110 0.13 -1.1 860,109 + IAI47_04110 0.13 -0.5 860,109 + IAI47_04110 0.13 -0.9 860,109 + IAI47_04110 0.13 +0.1 860,109 + IAI47_04110 0.13 -0.3 860,109 + IAI47_04110 0.13 -1.2 860,109 + IAI47_04110 0.13 -1.4 860,110 - IAI47_04110 0.13 +0.4 860,110 - IAI47_04110 0.13 +1.0 860,110 - IAI47_04110 0.13 -0.0 860,110 - IAI47_04110 0.13 -1.6 860,110 - IAI47_04110 0.13 -1.5 860,110 - IAI47_04110 0.13 -1.6 860,110 - IAI47_04110 0.13 -0.8 860,150 + IAI47_04110 0.14 -0.4 860,150 + IAI47_04110 0.14 +1.0 860,150 + IAI47_04110 0.14 -1.0 860,151 - IAI47_04110 0.14 +0.2 860,152 + IAI47_04110 0.14 -1.5 860,152 + IAI47_04110 0.14 -0.4 860,152 + IAI47_04110 0.14 -0.0 860,152 + IAI47_04110 0.14 +0.3 860,153 - IAI47_04110 0.14 +0.8 860,173 - IAI47_04110 0.15 -2.2 860,173 - IAI47_04110 0.15 -0.3 860,203 + IAI47_04110 0.16 +0.5 860,203 + IAI47_04110 0.16 -1.8 860,203 + IAI47_04110 0.16 -2.1 860,203 + IAI47_04110 0.16 -0.6 860,204 - IAI47_04110 0.16 -1.9 860,204 - IAI47_04110 0.16 -2.7 860,204 - IAI47_04110 0.16 -2.6 860,306 + IAI47_04110 0.20 -1.7 860,348 + IAI47_04110 0.21 +0.7 860,348 + IAI47_04110 0.21 -1.9 860,348 + IAI47_04110 0.21 -0.1 860,348 + IAI47_04110 0.21 -1.3 860,393 + IAI47_04110 0.23 -1.3 860,394 - IAI47_04110 0.23 -0.4 860,462 + IAI47_04110 0.26 -0.6 860,463 - IAI47_04110 0.26 -1.4 860,463 - IAI47_04110 0.26 -0.6 860,463 - IAI47_04110 0.26 -0.6 860,463 - IAI47_04110 0.26 +1.1 860,477 + IAI47_04110 0.26 -1.4 860,478 - IAI47_04110 0.26 -0.6 860,478 - IAI47_04110 0.26 +0.3 860,478 - IAI47_04110 0.26 -1.3 860,478 - IAI47_04110 0.26 -1.3 860,557 - IAI47_04110 0.29 +0.4
Or see this region's nucleotide sequence