Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_02625 and IAI47_02630 are separated by 68 nucleotides IAI47_02630 and IAI47_02635 are separated by 220 nucleotides
IAI47_02625: IAI47_02625 - ribonuclease R, at 542,865 to 545,354
_02625
IAI47_02630: IAI47_02630 - 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB, at 545,423 to 546,157
_02630
IAI47_02635: IAI47_02635 - isovaleryl-CoA dehydrogenase, at 546,378 to 548,006
_02635
Position (kb) 545
546
547 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 544.506 kb on + strand, within IAI47_02625 at 544.507 kb on - strand, within IAI47_02625 at 544.551 kb on + strand, within IAI47_02625 at 544.551 kb on + strand, within IAI47_02625 at 544.552 kb on - strand, within IAI47_02625 at 544.552 kb on - strand, within IAI47_02625 at 544.552 kb on - strand, within IAI47_02625 at 544.562 kb on + strand, within IAI47_02625 at 544.563 kb on - strand, within IAI47_02625 at 544.614 kb on + strand, within IAI47_02625 at 544.614 kb on + strand, within IAI47_02625 at 544.615 kb on - strand, within IAI47_02625 at 544.615 kb on - strand, within IAI47_02625 at 544.615 kb on - strand, within IAI47_02625 at 544.671 kb on + strand, within IAI47_02625 at 544.671 kb on + strand, within IAI47_02625 at 544.671 kb on + strand, within IAI47_02625 at 544.671 kb on + strand, within IAI47_02625 at 544.710 kb on - strand, within IAI47_02625 at 544.731 kb on + strand, within IAI47_02625 at 544.732 kb on - strand, within IAI47_02625 at 544.732 kb on - strand, within IAI47_02625 at 544.732 kb on - strand, within IAI47_02625 at 544.835 kb on + strand, within IAI47_02625 at 544.836 kb on - strand, within IAI47_02625 at 544.836 kb on - strand, within IAI47_02625 at 544.910 kb on - strand, within IAI47_02625 at 544.962 kb on + strand, within IAI47_02625 at 544.962 kb on + strand, within IAI47_02625 at 544.962 kb on + strand, within IAI47_02625 at 544.962 kb on + strand, within IAI47_02625 at 544.963 kb on - strand, within IAI47_02625 at 544.963 kb on - strand, within IAI47_02625 at 544.963 kb on - strand, within IAI47_02625 at 544.963 kb on - strand, within IAI47_02625 at 544.963 kb on - strand, within IAI47_02625 at 545.019 kb on + strand, within IAI47_02625 at 545.020 kb on - strand, within IAI47_02625 at 545.020 kb on - strand, within IAI47_02625 at 545.076 kb on - strand, within IAI47_02625 at 545.076 kb on - strand, within IAI47_02625 at 545.127 kb on - strand at 545.201 kb on + strand at 545.202 kb on - strand at 545.234 kb on + strand at 545.234 kb on + strand at 545.234 kb on + strand at 545.235 kb on - strand at 545.267 kb on + strand at 545.395 kb on + strand at 545.396 kb on - strand at 545.396 kb on - strand at 545.396 kb on - strand at 545.434 kb on + strand at 545.501 kb on - strand, within IAI47_02630 at 545.501 kb on - strand, within IAI47_02630 at 545.570 kb on - strand, within IAI47_02630 at 545.570 kb on - strand, within IAI47_02630 at 545.629 kb on + strand, within IAI47_02630 at 545.635 kb on - strand, within IAI47_02630 at 545.635 kb on - strand, within IAI47_02630 at 545.715 kb on + strand, within IAI47_02630 at 545.715 kb on + strand, within IAI47_02630 at 545.715 kb on + strand, within IAI47_02630 at 545.716 kb on - strand, within IAI47_02630 at 545.716 kb on - strand, within IAI47_02630 at 545.716 kb on - strand, within IAI47_02630 at 545.744 kb on - strand, within IAI47_02630 at 545.744 kb on - strand, within IAI47_02630 at 545.900 kb on - strand, within IAI47_02630 at 545.921 kb on + strand, within IAI47_02630 at 545.921 kb on + strand, within IAI47_02630 at 545.921 kb on + strand, within IAI47_02630 at 545.921 kb on + strand, within IAI47_02630 at 545.921 kb on + strand, within IAI47_02630 at 545.921 kb on + strand, within IAI47_02630 at 545.921 kb on + strand, within IAI47_02630 at 545.922 kb on - strand, within IAI47_02630 at 545.922 kb on - strand, within IAI47_02630 at 545.922 kb on - strand, within IAI47_02630 at 545.922 kb on - strand, within IAI47_02630 at 545.922 kb on - strand, within IAI47_02630 at 545.922 kb on - strand, within IAI47_02630 at 545.922 kb on - strand, within IAI47_02630 at 545.923 kb on + strand, within IAI47_02630 at 545.923 kb on + strand, within IAI47_02630 at 545.923 kb on + strand, within IAI47_02630 at 545.923 kb on + strand, within IAI47_02630 at 545.923 kb on + strand, within IAI47_02630 at 545.923 kb on + strand, within IAI47_02630 at 545.924 kb on - strand, within IAI47_02630 at 545.924 kb on - strand, within IAI47_02630 at 545.924 kb on - strand, within IAI47_02630 at 545.924 kb on - strand, within IAI47_02630 at 545.924 kb on - strand, within IAI47_02630 at 545.924 kb on - strand, within IAI47_02630 at 545.924 kb on - strand, within IAI47_02630 at 545.962 kb on + strand, within IAI47_02630 at 545.962 kb on + strand, within IAI47_02630 at 545.962 kb on + strand, within IAI47_02630 at 545.963 kb on - strand, within IAI47_02630 at 545.963 kb on - strand, within IAI47_02630 at 546.184 kb on + strand at 546.185 kb on - strand at 546.193 kb on + strand at 546.193 kb on + strand at 546.202 kb on + strand at 546.202 kb on + strand at 546.203 kb on - strand at 546.203 kb on - strand at 546.203 kb on - strand at 546.203 kb on - strand at 546.203 kb on - strand at 546.203 kb on - strand at 546.203 kb on - strand at 546.219 kb on + strand at 546.219 kb on + strand at 546.219 kb on + strand at 546.219 kb on + strand at 546.219 kb on + strand at 546.219 kb on + strand at 546.219 kb on + strand at 546.219 kb on + strand at 546.219 kb on + strand at 546.219 kb on + strand at 546.220 kb on - strand at 546.220 kb on - strand at 546.220 kb on - strand at 546.220 kb on - strand at 546.220 kb on - strand at 546.220 kb on - strand at 546.220 kb on - strand at 546.221 kb on + strand at 546.221 kb on + strand at 546.221 kb on + strand at 546.221 kb on + strand at 546.221 kb on + strand at 546.221 kb on + strand at 546.221 kb on + strand at 546.222 kb on - strand at 546.222 kb on - strand at 546.222 kb on - strand at 546.222 kb on - strand at 546.222 kb on - strand at 546.222 kb on - strand at 546.223 kb on + strand at 546.223 kb on + strand at 546.223 kb on + strand at 546.223 kb on + strand at 546.223 kb on + strand at 546.223 kb on + strand at 546.224 kb on - strand at 546.224 kb on - strand at 546.224 kb on - strand at 546.224 kb on - strand at 546.279 kb on + strand at 546.279 kb on + strand at 546.279 kb on + strand at 546.279 kb on + strand at 546.279 kb on + strand at 546.279 kb on + strand at 546.279 kb on + strand at 546.279 kb on + strand at 546.279 kb on + strand at 546.279 kb on + strand at 546.279 kb on + strand at 546.279 kb on + strand at 546.279 kb on + strand at 546.279 kb on + strand at 546.279 kb on + strand at 546.280 kb on - strand at 546.280 kb on - strand at 546.280 kb on - strand at 546.280 kb on - strand at 546.280 kb on - strand at 546.280 kb on - strand at 546.280 kb on - strand at 546.280 kb on - strand at 546.280 kb on - strand at 546.280 kb on - strand at 546.280 kb on - strand at 546.280 kb on - strand at 546.280 kb on - strand at 546.280 kb on - strand at 546.280 kb on - strand at 546.280 kb on - strand at 546.280 kb on - strand at 546.280 kb on - strand at 546.287 kb on + strand at 546.287 kb on + strand at 546.287 kb on + strand at 546.287 kb on + strand at 546.287 kb on + strand at 546.288 kb on - strand at 546.288 kb on - strand at 546.288 kb on - strand at 546.288 kb on - strand at 546.288 kb on - strand at 546.288 kb on - strand at 546.288 kb on - strand at 546.288 kb on - strand at 546.288 kb on - strand at 546.288 kb on - strand at 546.288 kb on - strand at 546.345 kb on - strand at 546.346 kb on + strand at 546.346 kb on + strand at 546.346 kb on + strand at 546.346 kb on + strand at 546.346 kb on + strand at 546.346 kb on + strand at 546.346 kb on + strand at 546.346 kb on + strand at 546.346 kb on + strand at 546.346 kb on + strand at 546.347 kb on - strand at 546.347 kb on - strand at 546.347 kb on - strand at 546.347 kb on - strand at 546.347 kb on - strand at 546.347 kb on - strand at 546.347 kb on - strand at 546.347 kb on - strand at 546.347 kb on - strand at 546.347 kb on - strand at 546.347 kb on - strand at 546.347 kb on - strand at 546.347 kb on - strand at 546.347 kb on - strand at 546.347 kb on - strand at 546.350 kb on + strand at 546.350 kb on + strand at 546.350 kb on + strand at 546.350 kb on + strand at 546.350 kb on + strand at 546.351 kb on - strand at 546.351 kb on - strand at 546.351 kb on - strand at 546.351 kb on - strand at 546.351 kb on - strand at 546.351 kb on - strand at 546.351 kb on - strand at 546.405 kb on - strand at 546.449 kb on + strand at 546.514 kb on + strand at 546.514 kb on + strand at 546.514 kb on + strand at 546.515 kb on - strand at 546.515 kb on - strand at 546.515 kb on - strand at 546.740 kb on + strand, within IAI47_02635 at 546.741 kb on - strand, within IAI47_02635 at 546.811 kb on + strand, within IAI47_02635 at 546.811 kb on + strand, within IAI47_02635 at 546.811 kb on + strand, within IAI47_02635 at 546.811 kb on + strand, within IAI47_02635 at 546.811 kb on + strand, within IAI47_02635 at 546.812 kb on - strand, within IAI47_02635 at 546.812 kb on - strand, within IAI47_02635 at 546.819 kb on + strand, within IAI47_02635 at 546.819 kb on + strand, within IAI47_02635 at 546.819 kb on + strand, within IAI47_02635 at 546.819 kb on + strand, within IAI47_02635 at 546.819 kb on + strand, within IAI47_02635 at 546.819 kb on + strand, within IAI47_02635 at 546.819 kb on + strand, within IAI47_02635 at 546.819 kb on + strand, within IAI47_02635 at 546.820 kb on - strand, within IAI47_02635 at 546.870 kb on + strand, within IAI47_02635 at 546.927 kb on - strand, within IAI47_02635 at 547.011 kb on + strand, within IAI47_02635 at 547.012 kb on - strand, within IAI47_02635 at 547.028 kb on + strand, within IAI47_02635 at 547.028 kb on + strand, within IAI47_02635 at 547.029 kb on - strand, within IAI47_02635 at 547.029 kb on - strand, within IAI47_02635 at 547.093 kb on + strand, within IAI47_02635 at 547.094 kb on - strand, within IAI47_02635 at 547.094 kb on - strand, within IAI47_02635 at 547.094 kb on - strand, within IAI47_02635 at 547.094 kb on - strand, within IAI47_02635
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 544,506 + IAI47_02625 0.66 +1.1 544,507 - IAI47_02625 0.66 +0.7 544,551 + IAI47_02625 0.68 -0.3 544,551 + IAI47_02625 0.68 +0.2 544,552 - IAI47_02625 0.68 -0.3 544,552 - IAI47_02625 0.68 +1.5 544,552 - IAI47_02625 0.68 -1.6 544,562 + IAI47_02625 0.68 +0.1 544,563 - IAI47_02625 0.68 -0.5 544,614 + IAI47_02625 0.70 -0.7 544,614 + IAI47_02625 0.70 +1.4 544,615 - IAI47_02625 0.70 -2.7 544,615 - IAI47_02625 0.70 -0.0 544,615 - IAI47_02625 0.70 +0.8 544,671 + IAI47_02625 0.73 -0.5 544,671 + IAI47_02625 0.73 +0.9 544,671 + IAI47_02625 0.73 +1.2 544,671 + IAI47_02625 0.73 +0.2 544,710 - IAI47_02625 0.74 +0.4 544,731 + IAI47_02625 0.75 -0.2 544,732 - IAI47_02625 0.75 -1.9 544,732 - IAI47_02625 0.75 -0.0 544,732 - IAI47_02625 0.75 -0.3 544,835 + IAI47_02625 0.79 +0.0 544,836 - IAI47_02625 0.79 +2.2 544,836 - IAI47_02625 0.79 -1.0 544,910 - IAI47_02625 0.82 -1.1 544,962 + IAI47_02625 0.84 -0.8 544,962 + IAI47_02625 0.84 +0.6 544,962 + IAI47_02625 0.84 -0.2 544,962 + IAI47_02625 0.84 +0.9 544,963 - IAI47_02625 0.84 +0.0 544,963 - IAI47_02625 0.84 +0.0 544,963 - IAI47_02625 0.84 +1.7 544,963 - IAI47_02625 0.84 -1.7 544,963 - IAI47_02625 0.84 -0.2 545,019 + IAI47_02625 0.87 -0.6 545,020 - IAI47_02625 0.87 +2.5 545,020 - IAI47_02625 0.87 +0.8 545,076 - IAI47_02625 0.89 +0.4 545,076 - IAI47_02625 0.89 -1.5 545,127 - +1.1 545,201 + -0.1 545,202 - -1.2 545,234 + -0.6 545,234 + -0.6 545,234 + +1.9 545,235 - +2.4 545,267 + +1.0 545,395 + -0.5 545,396 - -1.3 545,396 - +2.0 545,396 - +0.2 545,434 + -0.9 545,501 - IAI47_02630 0.11 +1.2 545,501 - IAI47_02630 0.11 -2.5 545,570 - IAI47_02630 0.20 +1.0 545,570 - IAI47_02630 0.20 +1.3 545,629 + IAI47_02630 0.28 -0.3 545,635 - IAI47_02630 0.29 +0.9 545,635 - IAI47_02630 0.29 +1.8 545,715 + IAI47_02630 0.40 +1.3 545,715 + IAI47_02630 0.40 +0.4 545,715 + IAI47_02630 0.40 +1.0 545,716 - IAI47_02630 0.40 -0.1 545,716 - IAI47_02630 0.40 +1.1 545,716 - IAI47_02630 0.40 +0.4 545,744 - IAI47_02630 0.44 +1.5 545,744 - IAI47_02630 0.44 +0.2 545,900 - IAI47_02630 0.65 +1.5 545,921 + IAI47_02630 0.68 -1.3 545,921 + IAI47_02630 0.68 +1.4 545,921 + IAI47_02630 0.68 -0.5 545,921 + IAI47_02630 0.68 +0.2 545,921 + IAI47_02630 0.68 +0.1 545,921 + IAI47_02630 0.68 +0.1 545,921 + IAI47_02630 0.68 +0.0 545,922 - IAI47_02630 0.68 +0.1 545,922 - IAI47_02630 0.68 +0.7 545,922 - IAI47_02630 0.68 -1.8 545,922 - IAI47_02630 0.68 +3.5 545,922 - IAI47_02630 0.68 +1.9 545,922 - IAI47_02630 0.68 +0.9 545,922 - IAI47_02630 0.68 -0.2 545,923 + IAI47_02630 0.68 +1.4 545,923 + IAI47_02630 0.68 -0.3 545,923 + IAI47_02630 0.68 -0.3 545,923 + IAI47_02630 0.68 +0.1 545,923 + IAI47_02630 0.68 +1.4 545,923 + IAI47_02630 0.68 +1.3 545,924 - IAI47_02630 0.68 +3.3 545,924 - IAI47_02630 0.68 -0.5 545,924 - IAI47_02630 0.68 +0.5 545,924 - IAI47_02630 0.68 -0.5 545,924 - IAI47_02630 0.68 +2.2 545,924 - IAI47_02630 0.68 +1.0 545,924 - IAI47_02630 0.68 -0.5 545,962 + IAI47_02630 0.73 +1.1 545,962 + IAI47_02630 0.73 -2.5 545,962 + IAI47_02630 0.73 +2.4 545,963 - IAI47_02630 0.73 +0.2 545,963 - IAI47_02630 0.73 +1.9 546,184 + -1.5 546,185 - +0.4 546,193 + -1.7 546,193 + +0.4 546,202 + +0.2 546,202 + -0.8 546,203 - +0.3 546,203 - -2.3 546,203 - +0.4 546,203 - -1.6 546,203 - -0.5 546,203 - +0.5 546,203 - +0.9 546,219 + -0.8 546,219 + +1.5 546,219 + +0.8 546,219 + -1.0 546,219 + +1.0 546,219 + -0.6 546,219 + -0.8 546,219 + -0.5 546,219 + -1.2 546,219 + -1.2 546,220 - -1.4 546,220 - -0.2 546,220 - +1.3 546,220 - -1.3 546,220 - +0.0 546,220 - +1.4 546,220 - -0.3 546,221 + -0.0 546,221 + +1.4 546,221 + +0.1 546,221 + +1.1 546,221 + +0.6 546,221 + -0.8 546,221 + +1.1 546,222 - +1.5 546,222 - +0.8 546,222 - +2.1 546,222 - -1.9 546,222 - -1.5 546,222 - +0.3 546,223 + -0.4 546,223 + -0.3 546,223 + -2.1 546,223 + +0.8 546,223 + -1.1 546,223 + +0.8 546,224 - +1.3 546,224 - +1.4 546,224 - +1.0 546,224 - +0.2 546,279 + -1.4 546,279 + +0.1 546,279 + -1.0 546,279 + -1.4 546,279 + -1.3 546,279 + -0.9 546,279 + +1.1 546,279 + -1.1 546,279 + +0.5 546,279 + +2.6 546,279 + +0.9 546,279 + +2.8 546,279 + +0.6 546,279 + +0.2 546,279 + -1.2 546,280 - -1.2 546,280 - +1.2 546,280 - -1.1 546,280 - -2.2 546,280 - +1.5 546,280 - -0.8 546,280 - +0.7 546,280 - +2.3 546,280 - -1.0 546,280 - +0.4 546,280 - -1.6 546,280 - +1.2 546,280 - +0.2 546,280 - +2.0 546,280 - -2.4 546,280 - -0.5 546,280 - -1.8 546,280 - -0.1 546,287 + +0.3 546,287 + -0.3 546,287 + -0.2 546,287 + -2.3 546,287 + -0.4 546,288 - -1.6 546,288 - -2.1 546,288 - -1.5 546,288 - -0.3 546,288 - +0.5 546,288 - -2.3 546,288 - +0.5 546,288 - -0.4 546,288 - +0.3 546,288 - +0.7 546,288 - -0.2 546,345 - -0.1 546,346 + -1.7 546,346 + +0.1 546,346 + -0.2 546,346 + -0.5 546,346 + +0.5 546,346 + +1.3 546,346 + -0.6 546,346 + +0.2 546,346 + +1.1 546,346 + +1.7 546,347 - +0.6 546,347 - -0.6 546,347 - -0.2 546,347 - +1.8 546,347 - +0.3 546,347 - -1.2 546,347 - -1.4 546,347 - -1.0 546,347 - +2.1 546,347 - -2.3 546,347 - -0.9 546,347 - -1.6 546,347 - +0.1 546,347 - +0.6 546,347 - -0.3 546,350 + +2.0 546,350 + -0.6 546,350 + -0.5 546,350 + -0.3 546,350 + +0.6 546,351 - -1.0 546,351 - -0.1 546,351 - -0.0 546,351 - -2.7 546,351 - +0.5 546,351 - -1.4 546,351 - -1.1 546,405 - +1.7 546,449 + +1.1 546,514 + +0.4 546,514 + -0.6 546,514 + +1.2 546,515 - -0.9 546,515 - -2.1 546,515 - -0.8 546,740 + IAI47_02635 0.22 +1.0 546,741 - IAI47_02635 0.22 +0.8 546,811 + IAI47_02635 0.27 +0.8 546,811 + IAI47_02635 0.27 +1.2 546,811 + IAI47_02635 0.27 +1.0 546,811 + IAI47_02635 0.27 -1.2 546,811 + IAI47_02635 0.27 +0.5 546,812 - IAI47_02635 0.27 +1.5 546,812 - IAI47_02635 0.27 +0.9 546,819 + IAI47_02635 0.27 -1.5 546,819 + IAI47_02635 0.27 +0.8 546,819 + IAI47_02635 0.27 +0.6 546,819 + IAI47_02635 0.27 -2.1 546,819 + IAI47_02635 0.27 +0.2 546,819 + IAI47_02635 0.27 +1.8 546,819 + IAI47_02635 0.27 -1.2 546,819 + IAI47_02635 0.27 +0.5 546,820 - IAI47_02635 0.27 +0.7 546,870 + IAI47_02635 0.30 -0.3 546,927 - IAI47_02635 0.34 -0.5 547,011 + IAI47_02635 0.39 +1.2 547,012 - IAI47_02635 0.39 +0.8 547,028 + IAI47_02635 0.40 -1.8 547,028 + IAI47_02635 0.40 -1.1 547,029 - IAI47_02635 0.40 +0.2 547,029 - IAI47_02635 0.40 +0.0 547,093 + IAI47_02635 0.44 -1.3 547,094 - IAI47_02635 0.44 +0.2 547,094 - IAI47_02635 0.44 +0.0 547,094 - IAI47_02635 0.44 -1.1 547,094 - IAI47_02635 0.44 -0.3
Or see this region's nucleotide sequence