Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_10820 and IAI47_10825 are separated by 152 nucleotides IAI47_10825 and IAI47_10830 are separated by 3 nucleotides IAI47_10830 and IAI47_10835 are separated by 11 nucleotides IAI47_10835 and IAI47_10840 are separated by 345 nucleotides
IAI47_10820: IAI47_10820 - MATE family efflux transporter, at 2,284,975 to 2,286,348
_10820
IAI47_10825: IAI47_10825 - tRNA-Val, at 2,286,501 to 2,286,577
_10825
IAI47_10830: IAI47_10830 - tRNA-Val, at 2,286,581 to 2,286,657
_10830
IAI47_10835: IAI47_10835 - tRNA-Val, at 2,286,669 to 2,286,745
_10835
IAI47_10840: IAI47_10840 - pyruvate kinase PykF, at 2,287,091 to 2,288,503
_10840
Position (kb)
2286
2287 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2286.004 kb on + strand, within IAI47_10820 at 2286.004 kb on + strand, within IAI47_10820 at 2286.004 kb on + strand, within IAI47_10820 at 2286.004 kb on + strand, within IAI47_10820 at 2286.004 kb on + strand, within IAI47_10820 at 2286.004 kb on + strand, within IAI47_10820 at 2286.005 kb on - strand, within IAI47_10820 at 2286.005 kb on - strand, within IAI47_10820 at 2286.005 kb on - strand, within IAI47_10820 at 2286.005 kb on - strand, within IAI47_10820 at 2286.006 kb on + strand, within IAI47_10820 at 2286.012 kb on + strand, within IAI47_10820 at 2286.013 kb on - strand, within IAI47_10820 at 2286.013 kb on - strand, within IAI47_10820 at 2286.013 kb on - strand, within IAI47_10820 at 2286.064 kb on + strand, within IAI47_10820 at 2286.083 kb on + strand, within IAI47_10820 at 2286.083 kb on + strand, within IAI47_10820 at 2286.115 kb on + strand, within IAI47_10820 at 2286.116 kb on - strand, within IAI47_10820 at 2286.117 kb on + strand, within IAI47_10820 at 2286.117 kb on + strand, within IAI47_10820 at 2286.117 kb on + strand, within IAI47_10820 at 2286.118 kb on - strand, within IAI47_10820 at 2286.123 kb on + strand, within IAI47_10820 at 2286.123 kb on + strand, within IAI47_10820 at 2286.123 kb on + strand, within IAI47_10820 at 2286.124 kb on - strand, within IAI47_10820 at 2286.124 kb on - strand, within IAI47_10820 at 2286.144 kb on + strand, within IAI47_10820 at 2286.144 kb on + strand, within IAI47_10820 at 2286.145 kb on - strand, within IAI47_10820 at 2286.145 kb on - strand, within IAI47_10820 at 2286.157 kb on + strand, within IAI47_10820 at 2286.157 kb on + strand, within IAI47_10820 at 2286.158 kb on - strand, within IAI47_10820 at 2286.185 kb on - strand, within IAI47_10820 at 2286.249 kb on + strand at 2286.252 kb on + strand at 2286.252 kb on + strand at 2286.292 kb on - strand at 2286.302 kb on + strand at 2286.303 kb on - strand at 2286.346 kb on + strand at 2286.347 kb on - strand at 2286.347 kb on - strand at 2286.347 kb on - strand at 2286.433 kb on - strand at 2286.433 kb on - strand at 2286.508 kb on + strand at 2286.508 kb on + strand at 2286.508 kb on + strand at 2286.508 kb on + strand at 2286.508 kb on + strand at 2286.521 kb on + strand, within IAI47_10825 at 2286.521 kb on + strand, within IAI47_10825 at 2286.566 kb on + strand, within IAI47_10825 at 2286.659 kb on + strand at 2286.659 kb on + strand at 2286.659 kb on + strand at 2286.659 kb on + strand at 2286.659 kb on + strand at 2286.659 kb on + strand at 2286.663 kb on + strand at 2286.663 kb on + strand at 2286.663 kb on + strand at 2286.664 kb on - strand at 2286.666 kb on + strand at 2286.666 kb on + strand at 2286.666 kb on + strand at 2286.667 kb on - strand at 2286.677 kb on - strand, within IAI47_10835 at 2286.832 kb on - strand at 2286.835 kb on + strand at 2286.835 kb on + strand at 2286.836 kb on - strand at 2286.844 kb on + strand at 2286.844 kb on + strand at 2286.844 kb on + strand at 2286.844 kb on + strand at 2286.844 kb on + strand at 2286.844 kb on + strand at 2286.861 kb on + strand at 2286.861 kb on + strand at 2286.861 kb on + strand at 2286.862 kb on - strand at 2286.890 kb on + strand at 2286.890 kb on + strand at 2286.890 kb on + strand at 2286.890 kb on + strand at 2286.891 kb on - strand at 2286.891 kb on - strand at 2286.891 kb on - strand at 2286.909 kb on - strand at 2286.982 kb on + strand at 2286.982 kb on + strand at 2286.982 kb on + strand at 2286.982 kb on + strand at 2286.982 kb on + strand at 2286.983 kb on - strand at 2286.989 kb on - strand at 2286.996 kb on - strand at 2287.189 kb on + strand at 2287.189 kb on + strand at 2287.237 kb on + strand, within IAI47_10840 at 2287.237 kb on + strand, within IAI47_10840 at 2287.237 kb on + strand, within IAI47_10840 at 2287.238 kb on - strand, within IAI47_10840 at 2287.288 kb on + strand, within IAI47_10840 at 2287.364 kb on - strand, within IAI47_10840 at 2287.364 kb on - strand, within IAI47_10840 at 2287.370 kb on - strand, within IAI47_10840 at 2287.370 kb on - strand, within IAI47_10840 at 2287.414 kb on + strand, within IAI47_10840 at 2287.414 kb on + strand, within IAI47_10840 at 2287.418 kb on + strand, within IAI47_10840 at 2287.419 kb on - strand, within IAI47_10840 at 2287.510 kb on + strand, within IAI47_10840 at 2287.523 kb on - strand, within IAI47_10840 at 2287.675 kb on + strand, within IAI47_10840
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 2,286,004 + IAI47_10820 0.75 -0.5 2,286,004 + IAI47_10820 0.75 -0.1 2,286,004 + IAI47_10820 0.75 -1.1 2,286,004 + IAI47_10820 0.75 +1.3 2,286,004 + IAI47_10820 0.75 +2.6 2,286,004 + IAI47_10820 0.75 +1.9 2,286,005 - IAI47_10820 0.75 -1.2 2,286,005 - IAI47_10820 0.75 +1.0 2,286,005 - IAI47_10820 0.75 -0.7 2,286,005 - IAI47_10820 0.75 -0.8 2,286,006 + IAI47_10820 0.75 +2.5 2,286,012 + IAI47_10820 0.75 -2.3 2,286,013 - IAI47_10820 0.76 -0.2 2,286,013 - IAI47_10820 0.76 +1.7 2,286,013 - IAI47_10820 0.76 -0.7 2,286,064 + IAI47_10820 0.79 -0.8 2,286,083 + IAI47_10820 0.81 -0.0 2,286,083 + IAI47_10820 0.81 +0.8 2,286,115 + IAI47_10820 0.83 -2.9 2,286,116 - IAI47_10820 0.83 +0.0 2,286,117 + IAI47_10820 0.83 -0.3 2,286,117 + IAI47_10820 0.83 -0.3 2,286,117 + IAI47_10820 0.83 +1.1 2,286,118 - IAI47_10820 0.83 +0.8 2,286,123 + IAI47_10820 0.84 -0.2 2,286,123 + IAI47_10820 0.84 +0.7 2,286,123 + IAI47_10820 0.84 -1.7 2,286,124 - IAI47_10820 0.84 +1.0 2,286,124 - IAI47_10820 0.84 -0.9 2,286,144 + IAI47_10820 0.85 -1.6 2,286,144 + IAI47_10820 0.85 -0.5 2,286,145 - IAI47_10820 0.85 +2.3 2,286,145 - IAI47_10820 0.85 +1.2 2,286,157 + IAI47_10820 0.86 +1.9 2,286,157 + IAI47_10820 0.86 -1.1 2,286,158 - IAI47_10820 0.86 +1.6 2,286,185 - IAI47_10820 0.88 +0.4 2,286,249 + +0.4 2,286,252 + -2.3 2,286,252 + +0.2 2,286,292 - +2.1 2,286,302 + +0.8 2,286,303 - +1.2 2,286,346 + -0.6 2,286,347 - -1.3 2,286,347 - +0.8 2,286,347 - -0.3 2,286,433 - -0.7 2,286,433 - -1.0 2,286,508 + +0.6 2,286,508 + -1.1 2,286,508 + +0.2 2,286,508 + +1.6 2,286,508 + -1.3 2,286,521 + IAI47_10825 0.26 -0.7 2,286,521 + IAI47_10825 0.26 +0.0 2,286,566 + IAI47_10825 0.84 -0.3 2,286,659 + -1.3 2,286,659 + -2.9 2,286,659 + -0.1 2,286,659 + -1.0 2,286,659 + -0.4 2,286,659 + -1.8 2,286,663 + +1.0 2,286,663 + +1.2 2,286,663 + -3.1 2,286,664 - -0.2 2,286,666 + +1.7 2,286,666 + +1.0 2,286,666 + -0.8 2,286,667 - +0.5 2,286,677 - IAI47_10835 0.10 -1.2 2,286,832 - +0.0 2,286,835 + +1.9 2,286,835 + +2.1 2,286,836 - +1.3 2,286,844 + +1.4 2,286,844 + -0.8 2,286,844 + -0.2 2,286,844 + +1.2 2,286,844 + +0.6 2,286,844 + -0.8 2,286,861 + -0.2 2,286,861 + +0.2 2,286,861 + -2.0 2,286,862 - +0.0 2,286,890 + -0.6 2,286,890 + -2.6 2,286,890 + -2.1 2,286,890 + -1.8 2,286,891 - +0.7 2,286,891 - -0.7 2,286,891 - -0.6 2,286,909 - -0.7 2,286,982 + -0.7 2,286,982 + +0.1 2,286,982 + -2.5 2,286,982 + -1.2 2,286,982 + -0.7 2,286,983 - -1.1 2,286,989 - +1.4 2,286,996 - +0.2 2,287,189 + -0.4 2,287,189 + +3.6 2,287,237 + IAI47_10840 0.10 +1.2 2,287,237 + IAI47_10840 0.10 -0.7 2,287,237 + IAI47_10840 0.10 -0.2 2,287,238 - IAI47_10840 0.10 -0.2 2,287,288 + IAI47_10840 0.14 +1.2 2,287,364 - IAI47_10840 0.19 +1.0 2,287,364 - IAI47_10840 0.19 -0.4 2,287,370 - IAI47_10840 0.20 +0.7 2,287,370 - IAI47_10840 0.20 +0.3 2,287,414 + IAI47_10840 0.23 +2.2 2,287,414 + IAI47_10840 0.23 +1.8 2,287,418 + IAI47_10840 0.23 +0.7 2,287,419 - IAI47_10840 0.23 +1.6 2,287,510 + IAI47_10840 0.30 +1.2 2,287,523 - IAI47_10840 0.31 +0.8 2,287,675 + IAI47_10840 0.41 +2.6
Or see this region's nucleotide sequence